Dikmen, Aycan, di Villa Bianca, Roberta d’Emmanuele, Mitidieri, Emma, Donnarumma, Erminia, Sevin, Gülnur, Cirino, Giuseppe, Sorrentino, Raffaella, and Yetik-Anacak, Gunay
It has been extensively demonstrated that hydrogen sulfyde (H 2 S) is implicated is several physiological and pathological conditions (J Mol Med, 2012 Mar;90(3):255–63). In particular, it has been shown that H 2 S causes relaxation in human penile tissues (Proc Natl Acad Sci USA, 2009 Mar 17;106(11):4513–8) and inhibits phosphodiesterase (PDE) activity in vessels (ATVB 2010, Oct;30(10):1998–2004). Beside sildenafil increases H 2 S generation in human bladder (Eur Urol. 2012 Dec;62(6):1174–80) and tadalafil in myocardial tissues (Circulation 2009, 120:S31-S36). Therefore, our aim was to demonstrate the link between H 2 S and PDE-5 in mice corpus cavernosum tissues. Thus, we investigated the effects of sildenafil (10 μM, 0.5 h); PDE-5 inhibitor, on H 2 S production as well as the H 2 S -induced relaxations in mice penile tissues. Penile tissues from CD1 mouse corpus cavernosum (MCC) were used. Functional studies were performed by myograph in Krebs solution. Western blot analysis was performed in order to evaluate CBS and CSE expression and methylene blue assay for measurement of H 2 S levels. In order to investigate functional significance of H 2 S on sildenafil-induced augmentation of endothelial relaxation in MCC the sildenafil effect was evaluated on acetylcholine (ACh; 10–9-10–4 M), L-cysteine (10–6-10–3 M) and NaHS-induced relaxations in presence or not of CSE enzyme inhibitor PPG (10 μM, 0.5 h). In order to achieve this issue the H 2 S production in MCC tissues was also evaluated by incubating the penile tissue with sildenafil in presence or absence of the CSE inhibitor PPG (10 μM, 0.5 h) Both CBS and CSE were expressed in MCC and the enzymes efficiently converted L-cysteine into H 2 S. Further we showed that sildenafil caused a significant increase in H 2 S production and this augmentation was reversed by CSE inhibition. We found that sildenafil induced an increase in both ACh and L-cysteine–induced relaxations and these augmentations reversed by CSE inhibitor PPG in MCC pre-contracted with phenylephrine (3.10–5 M). Beside sildenafil did not significantly increase the NaHS –induced relaxations. Therefore we suggest that both gaseous transmitters NO and H 2 S affect sildenafil action. In particular our results demonstrate that sildenafil effect is partially mediated by H 2 S pathway. Thus, H 2 S signaling may represent a new mechanism involved in the effect of sildenafil on erectile dysfunction. [ABSTRACT FROM AUTHOR]