43 results on '"Shane, Michael"'
Search Results
2. Adult Renal Transplantation in a Patient 28 Years after Heart Transplantation as a Neonate for Hypoplastic Left Heart Syndrome.
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Licata, Joseph, Roberts, Shane Michael, Janicki, Piotr, and Bezinover, Dmitri
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HYPOPLASTIC left heart syndrome , *HEART transplantation , *KIDNEY transplantation , *NEWBORN infants - Abstract
We present a case of kidney transplantation in a 28-year-old patient who received a heart transplant at 7 weeks of age due to hypoplastic left heart syndrome. The patient's renal insufficiency was the result of chronic immunosuppression and hypertension. The almost 28-year-old graft demonstrated very good function. This patient represents as one of the longest pediatric cardiac graft recipients living without any significant functional limitations. [ABSTRACT FROM AUTHOR]
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- 2022
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3. Contrasting phosphorus sensitivity of two Australian native monocots adapted to different habitats.
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Albornoz, Felipe E., Shane, Michael W., and Lambers, Hans
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CONTRAST sensitivity (Vision) , *SPECIES distribution , *MONOCOTYLEDONS , *PLANT species , *PHOSPHORUS in soils - Abstract
Aims: Contrasting nutrient-acquisition strategies would explain why species differ in their distribution in relation to soil phosphorus (P) availability, promoting diversity. However, what drives the differential distribution of plant species with the same P-acquisition strategy remains poorly understood. Methods: We selected two Haemodoraceae species, Anigozanthos flavidus and Macropidia fuliginosa, to investigate physiological responses in non-mycorrhizal monocots adapted to different edaphic habitats that vary in P availability. Plants were grown in nutrient solution in large tanks at a range of P concentrations (0, 0.1, 1, 10 µM P). After seven months, we measured growth, photosynthetic rate, net P-uptake capacity, and leaf [P]. Results: Fresh weights of A. flavidus plants were highest at 1 µM P and lowest at 0 µM P. Fresh weights of M. fuliginosa plants were lowest at 10 µM P compared with those at other P levels. Rates of P uptake by A. flavidus showed a steady decline with increasing P level during growth from 0 to 1 µM P, and then a sharp decline from 1 to 10 µM P. Rates of P uptake in M. fuliginosa did not differ among growth P levels, except between 0 and 1 µM P. Both species showed a drastic increase in the concentration of both total P and inorganic P at 10 µM P. Conclusions: The results support our hypothesis that A. flavidus is efficient in down-regulating its P-uptake capacity, while M. fuliginosa is not. Thus, partly explaining the narrower and wider distribution of these species. [ABSTRACT FROM AUTHOR]
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- 2021
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4. Dupuytren's contracture treated with botulinum toxin A injection.
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Davis, Shane Michael and Yao Chang, Eric
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BOTULINUM toxin , *BOTULINUM A toxins , *INJECTIONS , *MYONEURAL junction , *ANALGESIA - Abstract
Dupuytren's contracture is a condition in which fibromatosis of the palmar and digital fascia can lead to painful contractures of the hand. Common conservative treatments are of limited efficacy, while invasive options have risk of adverse effects. A 57-year-old female presented with chronic, bilateral hand pain secondary to Dupuytren's contractures. Traditional conservative treatments did not provide relief. She received a series of three botulinum toxin A injections into the palmar fascia of both hands, resulting in four months of improved hand function and pain relief. This case presents a novel treatment for Dupuytren's contracture using botulinum toxin injection. Botulinum toxin has inhibitory effects on the neuromuscular junction and pain signaling pathways to relax muscles and reduce pain. We believe its action on this patient's intrinsic hand muscles resulted in her improved hand function. Botulinum toxin injection can be considered as a treatment option for Dupuytren's contracture. [ABSTRACT FROM AUTHOR]
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- 2020
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5. Light-dependent activation of phosphoenolpyruvate carboxylase by reversible phosphorylation in cluster roots of white lupin plants: diurnal control in response to photosynthate supply.
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Shane, Michael W., Feil, Regina, Lunn, John E., and Plaxton, William C.
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ROOT formation , *LUPINUS albus , *PHOSPHATE fertilizers , *PLANT nutrients , *NUTRIENT uptake - Abstract
* Background and Aims Phosphoenolpyruvate carboxylase (PEPC) is a tightly regulated enzyme that controls carbohydrate partitioning to organic acid anions (malate, citrate) excreted in copious amounts by cluster roots of inorganic phosphate (Pi)-deprived white lupin plants. Excreted malate and citrate solubilize otherwise inaccessible sources of mineralized soil Pi for plant uptake. The aim of this study was to test the hypotheses that (1) PEPC is post-translationally activated by reversible phosphorylation in cluster roots of illuminated white lupin plants, and (2) light-dependent phosphorylation of cluster root PEPC is associated with elevated intracellular levels of sucrose and its signalling metabolite, trehalose-6-phosphate. * Methods White lupin plants were cultivated hydroponically at low Pi levels (≤1 µM) and subjected to various light/ dark pretreatments. Cluster root PEPC activity and in vivo phosphorylation status were analysed to assess the enzyme's diurnal, post-translational control in response to light and dark. Levels of various metabolites, including sucrose and trehalose-6-phosphate, were also quantified in cluster root extracts using enzymatic and spectrometric methods. * Key Results During the daytime the cluster root PEPC was activated by phosphorylation at its conserved N-terminal seryl residue. Darkness triggered a progressive reduction in PEPC phosphorylation to undetectable levels, and this was correlated with 75-80 % decreases in concentrations of sucrose and trehalose-6-phosphate. * Conclusions Reversible, light-dependent regulatory PEPC phosphorylation occurs in cluster roots of Pi-deprived white lupin plants. This likely facilitates the well-documented light- and sucrose-dependent exudation of Pi-solubilizing organic acid anions by the cluster roots. PEPC's in vivo phosphorylation status appears to be modulated by sucrose translocated from CO2-fixing leaves into the non-photosynthetic cluster roots. [ABSTRACT FROM AUTHOR]
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- 2016
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6. Hydrocarbon-Water Adsorption and Simulation of Catalyzed Hydrocarbon Traps.
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Sharma, Manish and Shane, Michael
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OXIDATION of hydrocarbons , *ADSORPTION (Chemistry) , *ZEOLITE catalysts , *SIMULATION methods & models , *MONOLITHIC reactors , *LANGMUIR isotherms - Abstract
Catalyzed hydrocarbon traps (HC traps) are being developed to provide HC control during the cold start. HC traps are made up of an adsorbent material, such as a zeolite, and a three way catalyst (precious metal based) present on the same monolith to provide trapping and oxidation functions. Traps are typically formulated with a multi-layer washcoat structure with the catalyst layer on top, and the trapping material placed under. The HC traps operate by storing (trapping) the unburned hydrocarbon molecules in the adsorbent material at a lower temperature when adsorption is favored and oxidation does not occur, and subsequently releasing these trapped HCs when the catalyst has heated sufficiently to convert the HCs to carbon dioxide and water. This paper presents the results of a modeling, laboratory and vehicle study on the performance of HC traps. First we present procedure to evaluate trap performance in the laboratory that provides the kinetic parameters for HC adsorption. A mathematical model is presented that can be used to simulate the HC trap. The feedgas to the zeolite trap on vehicle contains both HCs and water, present in the vehicle exhaust. The various HC species and water compete for adsorption on the zeolites. Lab experiments conducted on zeolites to study the competitive adsorption of various HCs and water is presented. Experiments have been conducted where different HCs and water are sequentially adsorbed on the zeolite, and TPD conducted. For modeling, Langmuir isotherm is assumed to represent the adsorption phenomena on the zeolite sites. Exothermic effects of water adsorption on acidic zeolites have been presented. Details on the experiments done to estimate the heat of adsorption of water on zeolites, and the modeling results of water adsorption on zeolites have also been provided. [ABSTRACT FROM AUTHOR]
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- 2016
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7. A Bus Tracking and Planning System for CTfastrak.
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Shuju Wu, Carroll, Shane Michael, Boyd-Carter, Keith, and Krostoski, James
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MOBILE apps , *AUTOMOTIVE transportation , *DATABASES , *TERMINALS (Transportation) , *MOBILE computing , *TELECOMMUNICATION systems - Abstract
This paper presents the application design and development of a bus tracking and planning system that involves mobile application development, database and network communications. This system is specifically designed for a public transportation bus system similar to the CTfastrak. The developed system includes three modules, namely the bus-side application, server-side database and client-side mobile application. Network communication enables the interactions between the bus-side, server-side and client-side modules. The objective is two-fold: to provide the bus clients more convenience with bus schedules, real-time bus location and delay information so they can plan trips accordingly, and to provide useful information to the transportation center for management purpose. The developed system has been tested and demonstrated successfully. [ABSTRACT FROM AUTHOR]
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- 2014
8. Senescence-inducible cell wall and intracellular purple acid phosphatases: implications for phosphorus remobilization in Hakea prostrata (Proteaceae) and Arabidopsis thaliana (Brassicaceae).
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Shane, Michael W., Stigter, Kyla, Fedosejevs, Eric T., and Plaxton, William C.
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CELLULAR aging , *PLANT cell walls , *PURPLE acid phosphatases , *ARABIDOPSIS thaliana genetics , *RIBONUCLEASE regulation , *EFFECT of phosphorus on plants - Abstract
Targeting of senescence-inducible acid phosphatases and RNases to the cell wall and vacuolar compartments appears to make a crucial contribution to efficient P remobilization networks of senescing tissues of Hakea prostrata and Arabidopsis.Despite its agronomic importance, the metabolic networks mediating phosphorus (P) remobilization during plant senescence are poorly understood. Highly efficient P remobilization (~85%) from senescing leaves and proteoid roots of harsh hakea (Hakea prostrata), a native ‘extremophile’ plant of south-western Australia, was linked with striking up-regulation of cell wall-localized and intracellular acid phosphatase (APase) and RNase activities. Non-denaturing PAGE followed by in-gel APase activity staining revealed senescence-inducible 120kDa and 60kDa intracellular APase isoforms, whereas only the 120kDa isoform was detected in corresponding cell wall fractions. Kinetic and immunological properties of the 120kDa and 60kDa APases partially purified from senescing leaves indicated that they are purple acid phosphatases (PAPs). Results obtained with cell wall-targeted hydrolases of harsh hakea were corroborated using Arabidopsis thaliana in which an ~200% increase in cell wall APase activity during leaf senescence was paralleled by accumulation of immunoreactive 55kDa AtPAP26 polypeptides. Senescing leaves of an atpap26 T-DNA insertion mutant displayed a >90% decrease in cell wall APase activity. Previous research established that senescing leaves of atpap26 plants exhibited a similar reduction in intracellular (vacuolar) APase activity, while displaying markedly impaired P remobilization efficiency and delayed senescence. It is hypothesized that up-regulation and dual targeting of PAPs and RNases to the cell wall and vacuolar compartments make a crucial contribution to highly efficient P remobilization that dominates the P metabolism of senescing tissues of harsh hakea and Arabidopsis. To the best of the authors’ knowledge, the apparent contribution of cell wall-targeted hydrolases to remobilizing key macronutrients such as P during senescence has not been previously suggested. [ABSTRACT FROM AUTHOR]
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- 2014
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9. Nematic Liquid Crystals Embedded in Cubic Microlattices: Memory Effects and Bistable Pixels.
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Serra, Francesca, Eaton, Shane Michael, Cerbino, Roberto, Buscaglia, Marco, Cerullo, Giulio, Osellame, Roberto, and Bellini, Tommaso
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NEMATIC liquid crystals , *LIQUID crystals , *POLYMERIZATION , *POROUS materials , *COMPUTER simulation , *BISTABLE devices - Abstract
The confinement of liquid crystals in geometries with frustrating boundary conditions gives rise to nontrivial effects such as bistability and memory. It is shown that large memory effects arise when nematic liquid crystals are embedded in cubic micrometer-sized scaffolds made by two-photon polymerization. The electric field alignment of the liquid crystals inside the porous medium is maintained when the applied field is above a threshold (approximately 2 V per micrometer of cell thickness). The onset of the memory is an on/off type process for each individual pore of the scaffold, and the memory typically starts emerging in one region of the structure and then propagates. The global memory effects in porous structures with controlled geometry are enhanced with respect to the case of random porous structures. This work is a proof of the 'memory from topology' principle, which was previously suggested by computer simulations. These new materials can pave the way to new types of bistable displays. [ABSTRACT FROM AUTHOR]
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- 2013
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10. Reciprocal Control of Anaplerotic Phosphoenolpyruvate Carboxylase by in Vivo Monoubiquitination and Phosphorylation in Developing Proteoid Roots of Phosphate-Deficient Harsh Hakea.
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Shane, Michael W., Fedosejevs, Eric T., and Plaxton, William C.
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PHOSPHOENOLPYRUVIC carboxylase , *CHEMICAL composition of plants , *PHOSPHORUS , *ANIONS , *SHRUBS , *BIOAVAILABILITY , *RHIZOSPHERE , *POLYPEPTIDES , *PHOSPHORYLATION - Abstract
Accumulating evidence indicates important functions for phosphoenolpyruvate (PEP) carboxylase (PEPC) in inorganic phosphate (Pi)-starved plants. This includes controlling the production of organic acid anions (malate, citrate) that are excreted in copious amounts by proteoid roots of normycorrhizal species such as harsh hakea (Hakea prostrata). This, in turn, enhances the bioavailability of mineral-bound Pi by solubilizing Al3+, Fe3+ and Ca2+ phosphates in the rhizosphere. Harsh hake thrives in the nutrient-impoverished, ancient soils of southwestern Australia. Proteoid roots from Pi-starved harsh hakea were analyzed over 20 d of development to correlate changes in malate and citrate exudation with PEPC activity, posttranslational modifications (inhibitory monoubiquitination versus activatory phosphorylation), and kinetic/allosteric properties. Immature proteoid roots contained an equivalent ratio of monoubiquitinated 110-kD and phosphorylated 107-kD PEPC polypeptides (p110 and p107, respectively). PEPC purification, immunoblotting, and mass spectrometry indicated that p110 and p107 are subunits of a 430-kD heterotetramer and that they both originate from the same plant-type PEPC gene. Incubation with a deubiquitinating enzyme converted the p110:p107 PEPC heterotetramer of immature proteoid roots into a p107 homotetramer while significantly increasing the enzyme's activity under suboptimal but physiologically relevant assay conditions. Proteoid root maturation was paralleled by PEPC activation (e.g. reduced Km [PEP] coupled with elevated I50 [malate and Asp] values) via in vivo deubiquitination of p110 to p107, and subsequent phosphorylation of the deubiquitinated subunits. This novel mechanism of posttranslational control is hypothesized to contribute to the massive synthesis and excretion of organic acid anions that dominates the carbon metabolism of the mature proteoid roots. [ABSTRACT FROM AUTHOR]
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- 2013
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11. Cutting, Driving, Digging, and Harvesting: Re-masculinizing the Working-Class Heroic.
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Fleras, Augie and Dixon, Shane Michael
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MASCULINITY , *BLUE collar workers , *WORKING class on television , *SELF-control , *HEROES , *BLUE collar workers on television - Abstract
Television portrayals of working-class males in blue-collar jobs have in the past proven unflattering at best; demeaning at worst But a new breed of unscripted TV shows articulates a fundamentally different narrative about the unsung heroism of working-class men. This article explores the narratives and images associated with the re-masculinization of blue-collared working-class males as real men in contrast to conventional working-class misrepresentations as persons lacking self controt; motivation and commitment This genre of "macho" male programs constitutes a key ideological tool by which "hegemonic" narratives of conventional masculinity are internalized through the "pleasures of the media." The authors conclude that; despite the recent valorization of blue-collar values, contributions, and identities, representational distortions and content omissions persist in portraying workingclass realities. [ABSTRACT FROM AUTHOR]
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- 2011
12. Development and persistence of sandsheaths of Lyginia barbata (Restionaceae): relation to root structural development and longevity.
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Shane, Michael W., McCully, Margaret E., Canny, Martin J., Pate, John S., and Lambers, Hans
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RESTIONACEAE , *ROOT development , *PLANT cells & tissues , *DROUGHT-tolerant plants , *PLANT histochemistry , *ROOT hairs (Botany) - Abstract
Background and Aims Strongly coherent sandsheaths that envelop perennial roots of many monocotyledonous species of arid environments have been described for over a century. This study, for the first time, details the roles played by the structural development of the subtending roots in the formation and persistence of the sheaths. Methods The structural development of root tissues associated with persistent sandsheaths was studied in Lyginia barbata, native to the Western Australian sand plains. Cryo-scanning electron microscopy CSEM, optical microscopy and specific staining methods were applied to fresh, field material. The role of root hairs was clarified by monitoring sheath development in roots separated from the sand profile by fine mesh. Key Results and Conclusions The formation of the sheaths depends entirely on the numerous living root hairs which extend into the sand and track closely around individual grains enmeshing, by approx. 12 cm from the root tip, a volume of sand more than 14 times that of the subtending root. The longevity of the perennial sheaths depends on the subsequent development of the root hairs and of the epidermis and cortex. Before dying, the root hairs develop cellulosic walls approx. 3 µm thick, incrusted with ferulic acid and lignin, which persist for the life of the sheath. The dead hairs remain in place fused to a persistent platform of sclerified epidermis and outer cortex. The mature cortex comprises this platform, a wide, sclerified inner rim and a lysigenous central region – all dead tissue. We propose that the sandsheath/root hair/epidermis/cortex complex is a structural unit facilitating water and nutrient uptake while the tissues are alive, recycling scarce phosphorus during senescence, and forming, when dead, a persistent essential structure for maintenance of a functional stele in the perennial Lyginia roots. [ABSTRACT FROM AUTHOR]
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- 2011
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13. Seasonal water relations of Lyginia barbata (Southern rush) in relation to root xylem development and summer dormancy of root apices.
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Shane, Michael W., McCully, Margaret E., Canny, Martin J., Pate, John S., Huang, Cheng, Ngo, Hai, and Lambers, Hans
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PLANT-water relationships , *XYLEM , *ROOT formation , *SHOOT apexes , *DORMANCY in plants , *RESTIONACEAE , *PHOTOBIOLOGY , *PLANT cells & tissues , *DROUGHTS - Abstract
•Periods of dormancy in shallow roots allow perennial monocotyledons to establish deep root systems, but we know little about patterns of xylem maturation, water-transport capacities and associated economies in water use of growing and dormant roots. •Xylem development, anatomy, conductance and in situ cellular [K] and [Cl] were investigated in roots of field-grown Lyginia barbata (Restionaceae) in Mediterranean southwestern Australia. Parallel studies of gas exchange, culm relative water loss and soil water content were conducted. •Stomatal conductance and photosynthesis decreased during summer drought as soil profiles dried, but rates recovered when dormant roots became active with the onset of wetter conditions. Anatomical studies identified sites of close juxtaposition of phloem and xylem in dormant and growing roots. Ion data and dye tracing showed mature late metaxylem of growing roots was located ≥ 100 mm from the tip, but at only ≤ 10 mm for dormant roots. Dormant roots remained hydrated in dry soils (0.001–0.005 g g−1). •Effective regulation of growth and water-conserving/obtaining properties permits the survival of shallow roots of L. barbata during summer drought and may represent important strategies for establishing deeper perennial root systems in other monocotyledonous plants adapted to seasonally dry habitats. [ABSTRACT FROM AUTHOR]
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- 2010
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14. Summer dormancy and winter growth: root survival strategy in a perennial monocotyledon.
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Shane, Michael W., McCully, Margaret E., Canny, Martin J., Pate, John S., Ngo, Hai, Mathesius, Ulrike, Cawthray, Gregory R., and Lambers, Hans
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DORMANCY in plants , *PLANT root ecology , *PLANT physiology , *PLANT development , *MONOCOTYLEDONS , *PERENNIALS , *RESTIONACEAE , *COMPARATIVE studies , *WINTER - Abstract
• Here, we tested the alternation of root summer dormancy and winter growth as a critical survival strategy for a long-lived monocotyledon (Restionaceae) adapted to harsh seasonal extremes of Mediterranean southwest Western Australia. • Measurements of growth and the results of comparative studies of the physiology, water content, metabolites, osmotic adjustments, and proteomics of the dormant and growing perennial roots of Lyginia barbata (Restionaceae) were assessed in field-grown plants. • Formation of dormant roots occurred before the onset of summer extremes. They resumed growth (average 2.3 mm d−1) the following winter to eventually reach depths of 2–4 m. Compared with winter-growing roots, summer dormant roots had decreased respiration and protein concentration and c. 70% water content, sustained by sand-sheaths, osmotic adjustment and presumably hydraulic redistribution. Concentrations of compatible solutes (e.g. sucrose and proline) were significantly greater during dormancy, presumably mitigating the effects of heat and drought. Fifteen root proteins showed differential abundance and were correlated with either winter growth or summer dormancy. None matched currently available libraries. • The specific features of the root dormancy strategy of L. barbata revealed in this study are likely to be important to understanding similar behaviour in roots of many long-lived monocotyledons, including overwintering and oversummering crop species. [ABSTRACT FROM AUTHOR]
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- 2009
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15. Root of edaphically controlled Proteaceae turnover on the Agulhas Plain, South Africa: phosphate uptake regulation and growth.
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SHANE, MICHAEL W., CRAMER, MICHAEL D., and LAMBERS, HANS
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PLANT roots , *PROTEACEAE , *PHOSPHATES , *PLANT growth , *PLANT physiology , *BOTANY - Abstract
The influence of phosphorus (P) availability on growth and P uptake was investigated in South African Proteaceae: (1) Protea compacta R.Br., endemic on severely nutrient-impoverished colluvial sands; (2) Protea obtusifolia Bueck ex Meissner; and (3) Leucadendron meridianum I. J. Williams, the latter both endemic on comparatively fertile limestone-derived soils. Plants were grown hydroponically in 1000 L tanks at 0.01, 0.1 or 1.0 µm P for 14 weeks. Biomass accumulation was influenced by P availability, doubling as [P] increased from 0.1 to 1.0 µm. Total biomass was greatest for P. compacta, but L. meridianum and P. obtusifolia had two to four times greater relative biomass accumulation at 0.1 and 1.0 µm[P]. Proteoid root clusters developed at both 0.01 and 0.1 µm[P], but were suppressed at 1.0 µm[P]; this was a 10-fold lower [P] than previously reported to inhibit cluster root formation. Rates of net P uptake at 5 µm P decreased in response to increased P availability from 0.01 to 1.0 µm P. Significant between-species differences in rates of P uptake and capacity to down-regulate P uptake were observed: P. compacta < P. obtusifolia < L. meridianum. The species responses are discussed in terms of adaptation to mosaics in soil P availability and the high beta diversity in the natural habitat. [ABSTRACT FROM AUTHOR]
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- 2008
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16. Specialized ‘dauciform’ roots of Cyperaceae are structurally distinct, but functionally analogous with ‘cluster’ roots.
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Shane, Michael W., Cawthray, Gregory R., Cramer, Michael D., Kuo, John, and Lambers, Hans
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PLANT root anatomy , *CITRATES , *PHOSPHORUS , *PLANT exudates , *CYPERACEAE , *PLANT cytochemistry - Abstract
When grown in nutrient solutions of extremely low [P] (≤1.0 µm), the sedge Schoenus unispiculatus Benth. (Cyperaceae) develops dauciform roots, which are short and carrot shaped, and produce dense numbers of long root hairs. It has been suggested that dauciform roots of monocotyledonous sedges function to acquire P from nutrient-poor, P-fixing soils in a manner similar to that of cluster (proteoid) roots developed by some dicotyledonous species, but without evidence to substantiate this claim. To elucidate the ecophysiological role of dauciform roots, we assessed carboxylate exudation, internal carboxylate and P concentrations and O2 uptake rates during dauciform root development. We showed that O2 consumption was fastest [9 nmol O2 g−1 fresh mass (FM) s−1] and root [P] greatest (0.4 mg P g−1 FM) when dauciform roots were young and rapidly developing. Citrate was the most abundant carboxylate in root tissues at all developmental stages, and was most concentrated (22.2 µmol citrate g−1 FM) in young dauciform roots, decreasing by more than half in mature dauciform roots. Peak citrate-exudation rates (1.7 nmol citrate g−1 FM s−1) occurred from mature dauciform roots, and were approximately an order of magnitude faster than those from roots of species without root clusters, and similar to those of mature proteoid (cluster) roots of Proteaceae. Both developing and mature dauciform roots had the capacity to acidify (but not alkalinize) the rhizosphere. Anatomical studies showed that epidermal cells in dauciform roots were greatly elongated in the transverse plane; epidermal cells of parent roots were unmodified. Although structurally distinct, the physiology of dauciform roots in sedges appears to be analogous to that of proteoid roots of Proteaceae and Fabaceae, and hence, dauciform roots would facilitate access to sorbed P and micronutrients from soils of low fertility. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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17. Hormonal regulation of the epithelial Na+ channel: From amphibians to mammals
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Shane, Michael Anne, Nofziger, Charity, and Blazer-Yost, Bonnie L.
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SODIUM channels , *EPITHELIUM , *CELL lines , *MAMMALS - Abstract
Abstract: High-resistance epithelia derived from amphibian sources such as frog skin, toad urinary bladder, and the A6 Xenopus laevis kidney cell line have been widely used to elucidate the underlying mechanisms involved in the regulation of vectorial ion transport. More recently, the isolation of high-resistance mammalian cell lines has provided model systems in which to study differences and similarities between the regulation of ion transporter function in amphibian and mammalian renal epithelia. In the present study, we have compared the natriferic (Na+ retaining) responses to aldosterone, insulin, and vasotocin/vasopressin in the A6 and mpkCCDcl4 (mouse principal cells of the kidney cortical collecting duct) cell lines. The functional responses of the epithelial Na+ channel (ENaC) to hormonal stimulation were remarkably similar in both the amphibian and mammalian lines. In addition, insulin- and aldosterone-stimulated, reabsorptive Na+ transport in both cell lines requires the presence of functional PI3-kinase. [Copyright &y& Elsevier]
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- 2006
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18. Systemic suppression of cluster-root formation and net P-uptake rates in Grevillea crithmifolia at elevated P supply: a proteacean with resistance for developing symptoms of ‘P toxicity’.
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Shane, Michael W. and Lambers, Hans
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GREVILLEA , *PLANT physiology , *PLANT development , *PLANT growth-promoting rhizobacteria , *REJUVENESCENCE (Botany) - Abstract
Grevillea crithmifolia R. Br. is a species of Proteaceae that is resistant to developing P-toxicity symptoms at phosphorus supplies in the root environment that induce P-toxicity symptoms in the closely related Hakea prostrata (Proteaceae). It was discovered previously that development of P-toxicity symptoms in H. prostrata is related to its low capacity to down-regulate net P-uptake rates (i.e. its low plasticity). The plasticity of net P-uptake rates and whole-plant growth responses in G. crithmifolia has now been assessed in two separate experiments: (i) a range of P, from 0 to 200 μmol P d−1, was supplied to whole root systems; (ii) using a split-root design, one root half was supplied with 0, 3, 75, or 225 μmol P d−1, while the other root half invariably received 3 μmol P d−1. Fresh mass was significantly greater in G. crithmifolia plants that had received a greater daily P supply during the pretreatments, but symptoms of P toxicity were never observed. Cluster-root growth decreased from about half the total root fresh mass when the leaf [P] was lowest (c. 0.1 mg P g−1 DM) to complete suppression of cluster-root growth when leaf [P] was 1–2 mg P g−1 DM. Split-root studies revealed that cluster-root initiation and growth, and net P-uptake rates by roots were regulated systemically, possibly by shoot P concentration. It is concluded that, in response to higher P supply, G. crithmifolia does not develop symptoms of P toxicity because of (i) greater plasticity of its net P-uptake capacity, and (ii) its greater plasticity for allocating P to growth and P storage in roots. This ecologically important difference in plasticity is most probably related to a slightly higher nutrient availability in the natural habitat of G. crithmifolia when compared with that of H. prostrata. [ABSTRACT FROM PUBLISHER]
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- 2006
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19. Manganese accumulation in leaves of Hakea prostrata (Proteaceae) and the significance of cluster roots for micronutrient uptake as dependent on phosphorus supply.
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Shane, Michael W. and Lambers, Hans
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PROTEACEAE , *PROTEALES , *MANGANESE , *PLANT roots , *PLANT nutrition , *PHOSPHORUS - Abstract
When grown at a low P supply, Hakea prostrata R.Br. (Proteaceae) develops dense clusters of determinate branch roots, termed ‘proteoid’ or ‘cluster’ roots and accumulates Mn in its leaves. The aim of this study was to vary the production of cluster roots and assess the relationship between Mn uptake and cluster-root mass. We collected native soil from a location inhabited by H. prostrata and amended this with ‘high’ and ‘low’ amounts of insoluble or soluble P. After 14 months, we measured the impact of the treatments on cluster-root development and the [P], [Mn], [Fe], [Zn] and [Cu] in young (expanding) and mature leaves. Dry mass and leaf area increased with increasing P availability in the soil, but growth decreased at the highest soluble [P], which caused symptoms of P toxicity. The [P] in young leaves (1.3–2.7 mg g−1 DM) exceeded that in older leaves (0.28–0.85 mg g−1 DM), except when plants were grown with soluble P (3.2–21 mg g−1 DM). Cluster-root formation was inhibited when leaf [P] increased; [P] in young leaves, rather than that in old leaves, appeared to be the factor that determined the proportion of the root mass invested in cluster roots. Old leaves of all treatments had [Mn] from 90 to 120 µg g−1 DM, except for plants grown at high levels of soluble P, when [Mn] decreased below 30 µg g−1 DM. The [Mn] and [Zn] in old leaves and the [Cu] in young leaves were positively correlated with the fraction of roots invested in cluster roots. These findings support our hypothesis that cluster roots play a significant role in micronutrient acquisition, and also provide an explanation for Mn accumulation in leaves of H. prostrata, and presumably Proteaceae in general. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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20. Cluster Roots: A Curiosity in Context.
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Shane, Michael W. and Lambers, Hans
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PLANT-soil relationships , *PLANT root physiology , *ROOT formation , *PLANT ecology - Abstract
Cluster roots are an adaptation for nutrient acquisition from nutrient-poor soils. They develop on root systems of a range of species belonging to a number of different families (e.g., Proteaceae, Casuarinaceae, Fabaceae and Myricaceae) and are also found on root systems of some crop species (e.g., albus, Macadamia integrifoliaand Cucurbita pepo). Their morphology is variable but typically, large numbers of determinate branch roots develop over very short distances of main root axes. Root clusters are ephemeral, and continually replaced by extension of the main root axes. Carboxylates are released from cluster roots at very fast rates for only a few days during a brief developmental window termed an ‘exudative burst’. Most of the studies of cluster-root metabolism have been carried out using the crop plant L. albus, but results on native plants have provided important additional information on carbon metabolism and exudate composition. Cluster-root forming species are generally non-mycorrhizal, and rely upon their specialised roots for the acquisition of phosphorus and other scarcely available nutrients. Phosphorus is a key plant nutrient for altering cluster-root formation, but their formation is also influenced by N and Fe. The initiation and growth of cluster roots is enhanced when plants are grown at a very low phosphate supply (viz. ≤1 μM P), and cluster-root suppression occurs at relatively higher P supplies. An important feature of some Proteaceae is storage of phosphorus in stem tissues which is associated with the seasonality of cluster-root development and P uptake (winter) and shoot growth (summer), and also maintains low leaf [P]. Some species of Proteaceae develop symptoms of P toxicity at relatively low external P supply. Our findings with Hakea prostrata (Proteaceae) indicate that P-toxicity symptoms result after the capacity of tissues to store P is exceeded. P accumulation in H. prostrata is due to its strongly decreased capacity to down-regulate P uptake when the external P supply is supra-optimal. The present review investigates cluster-root functioning in (1) L. albus (white lupin), the model crop plant for cluster-root studies, and (2) native Proteaceae that have evolved in phosphate-impoverished environments. [ABSTRACT FROM AUTHOR]
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- 2005
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21. The occurrence of dauciform roots amongst Western Australian reeds, rushes and sedges, and the impact of phosphorus supply on dauciform-root development in Schoenus unispiculatus (Cyperaceae).
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Shane, Michael W., Dixon, Kingsley W., and Lambers, Hans
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PLANT roots , *PHOSPHORUS , *CYPERACEAE , *LEAVES , *LUPINES - Abstract
• The incidence of species that develop specialised‘dauciform’ lateral roots, which are hypothesised to be important for phosphorus (P) acquisition, is uncertain. We investigated their occurrence in Australian reed, rush and sedge species, grown at low P concentration in nutrient solution, and studied the response ofSchoenus unispiculatus(Cyperaceae) to a range of P concentrations.• We assessed the fraction of root biomass invested in dauciform roots, their respiration and net P-uptake rate, and the P status of roots and leaves.• Dauciform-root development occurred only in particular genera of Cyperaceae when grown at low P supply. Increased P supply was associated with increased growth ofS. unispiculatusand increased leaf[P]. Dauciform-root growth was reduced by increased P supply, and reduced P uptake co-occurred with the complete suppression of dauciform roots.• The P-induced suppression of dauciform roots in Cyperaceae is similar to that observed for proteoid roots in members of Proteaceae andLupinus albus. The response of dauciform roots to altered P supply and their absence from root systems of some sedge species are discussed in terms of managed and natural systems.New Phytologist(2005)doi: 10.1111/j.1469-8137.2004.01283.x© New Phytologist(2005) [ABSTRACT FROM AUTHOR]
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- 2005
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22. Tissue and cellular phosphorus storage during development of phosphorus toxicity in Hakea prostrata (Proteaceae).
- Author
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Shane, Michael W., McCully, Margaret E., and Lambers, Hans
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PLANT development , *PLANT growth-promoting rhizobacteria , *PLANT growth , *PLANT shoots , *PROTEACEAE - Abstract
Storage of phosphorus (P) in stem tissue is important in Mediterranean Proteaceae, because proteoid root growth and P uptake is greatest during winter, whereas shoot growth occurs mostly in summer. This has prompted the present investigation of the P distribution amongst roots, stems, and leaves of Hakea prostrata R.Br. (Proteaceae) when grown in nutrient solutions at ten P‐supply rates. Glasshouse experiments were carried out during both winter and summer months. For plants grown in the low‐P range (0, 0.3, 1.2, 3.0, or 6.0 µmol d–1) the root [P] was > stem and leaf [P]. In contrast, leaf [P] > stem and root [P] for plants grown in the high‐P range (6.0, 30, 60, 150, or 300 µmol P d–1). At the highest P‐supply rates, the capacity for P storage in stems and roots appears to have been exceeded, and leaf [P] thereafter increased dramatically to approximately 10 mg P g–1 dry mass. This high leaf [P] was coincident with foliar symptoms of P toxicity which were similar to those described for many other species, including non‐Proteaceae. The published values (tissue [P]) at which P toxicity occurs in a range of species are summarized. X‐ray microanalysis of frozen, full‐hydrated leaves revealed that the [P] in vacuoles of epidermal, palisade and bundle‐sheath cells were in the mM range when plants were grown at low P‐supply, even though very low leaf [P] was measured in bulk leaf samples. At higher P‐supply rates, P accumulated in vacuoles of palisade cells which were associated with decreased photosynthetic rates. [ABSTRACT FROM PUBLISHER]
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- 2004
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23. Developmental Physiology of Cluster-Root Carboxylate Synthesis and Exudation in Harsh Hakea. Expression of Phosphoenolpyruvate Carboxylase and the Alternative Oxidase.
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Shane, Michael W., Cramer, Michael D., Funayama-Noguchi, Sachiko, Cawthray, Gregory R., Millar, A. Harvey, Day, David A., and Lambers, Hans
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OXIDASES , *PLANT growth , *PLANT physiology , *PROTEACEAE , *PLANT roots - Abstract
Harsh hakea (Hakea prostrata R.Br.) is a member of the Proteaceae family, which is highly represented on the extremely nutrientimpoverished soils in southwest Australia. When phosphorus is limiting, harsh hakea develops proteoid or cluster roots that release carboxylates that mobilize sparingly soluble phosphate in the rhizosphere. To investigate the physiology underlying the synthesis and exudation of carboxylates from cluster roots in Proteaceae, we measured O2 consumption, CO2 release, internal carboxylate concentrations and carboxylate exudation, and the abundance of the enzymes phosphoenolpyruvate carboxylase and alternative oxidase (AOX) over a 3-week time course of cluster-root development. Peak rates of citrate and malate exudation were observed from 12- to 13-d-old cluster roots, preceded by a reduction in cluster-root total protein levels and a reduced rate of O2 consumption. In harsh hakea, phosphoenolpyruvate carboxylase expression was relatively constant in cluster roots, regardless of developmental stage. During cluster-root maturation, however, the expression of AOX protein increased prior to the time when citrate and malate exudation peaked. This increase in AOX protein levels is presumably needed to allow a greater flow of electrons through the mitochondrial electron transport chain in the absence of rapid ATP turnover. Citrate and isocitrate synthesis and accumulation contributed in a major way to the subsequent burst of citrate and malate exudation. Phosphorus accumulated by harsh hakea cluster roots was remobilized during senescence as part of their efficient P cycling strategy for growth on nutrient impoverished soils. [ABSTRACT FROM AUTHOR]
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- 2004
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24. Effects of external phosphorus supply on internal phosphorus concentration and the initiation, growth and exudation of cluster roots in Hakea prostrata R.Br.
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Shane, Michael W., de Vos, Martin, de Roock, Sytze, Cawthray, Gregory R., and Lambers, Hans
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ROOT formation , *ROOT growth , *PLANT roots , *PHOSPHORUS , *EXUDATION (Botany) , *PLANT-soil relationships - Abstract
The response of internal phosphorus concentration, cluster-root initiation, and growth and carboxylate exudation to different external P supplies was investigated in Hakea prostrata R.Br. using a split-root design. After removal of most of the taproot, equal amounts of laterals were allowed to grow in two separate pots fastened together at the top, so that the separate root halves could be exposed to different conditions. Plants were grown for 10 weeks in this system; one root half was supplied with 1 μM P while the other halves were supplied with 0, 1, 25 or 75 μM P. Higher concentrations of P supplied to one root half significantly increased the P concentration of those roots and in the shoots. The P concentrations in root halves supplied with 1 μM P were invariably low, regardless of the P concentration supplied to the other root half. Cluster root initiation was completely suppressed on root halves supplied with 25 or 75 μM P, whereas it continued on the other halves supplied with 1 μM P indicating that cluster-root initiation was regulated by local root P concentration. Cluster-root growth (dry mass increment) on root halves supplied with 1 μM P was significantly reduced when the other half was either deprived of P or supplied with 25 or 75 μM P. Cluster-root growth was favoured by a low shoot P status at a root P supply that was adequate for increased growth of roots and shoots without increased tissue P concentrations. The differences in cluster-root growth on root halves with the same P supply suggest that decreased cluster-root growth was systemically regulated. Carboxylate-exudation rates from cluster roots on root halves supplied with 1 μM P were the same, whether the other root half was supplied with 1, 25 or 75 μM P, but were approximately 30 times faster when the other half was deprived of P. Estimates of root P-uptake rates suggest a rather limited capacity for down-regulating P uptake when phosphate was readily available. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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25. The zeta potential of phospors.
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Shane, Michael J.
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PHOSPHORS , *ZETA potential - Abstract
Presents a summary of the results of an investigation of the charging of phosphor particles in isopropyl alcohol (IPA). Measurement of particle velocity; Effect of nitrate salt concentration in IPA on zeta potential; Effect of pH on zeta potential of P-1 phosphor in IPA and IPA-containing magnesium nitrate; Effect of mechanical rolling chosen phospors on zeta potential.
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- 1993
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26. Liquid Crystals: Nematic Liquid Crystals Embedded in Cubic Microlattices: Memory Effects and Bistable Pixels (Adv. Funct. Mater. 32/2013).
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Serra, Francesca, Eaton, Shane Michael, Cerbino, Roberto, Buscaglia, Marco, Cerullo, Giulio, Osellame, Roberto, and Bellini, Tommaso
- Abstract
S. M. Eaton and co‐workers report the first experimental proof of the bistability of nematic liquid crystals incorporated in a porous network with a controlled geometry. This configuration, described in detail on page 3990, is specifically designed to enhance memory effects by inducing a regular arrangement of topological defects. The cover image shows this three dimensional porous network, fabricated by femtosecond laser lithography, with the incorporated liquid crystals. This composite material could be exploited for future ultralow‐power bistable displays. [ABSTRACT FROM AUTHOR]
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- 2013
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27. Optimizing marine stock enhancement through modeling: A sex-specific application with California halibut Paralichthys californicus.
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MacNamara, Ruairi, Camp, Edward, Shane, Michael, Lorenzen, Kai, and Drawbridge, Mark
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PARALICHTHYS , *JUVENILE offenders , *SEX ratio , *RESOURCE allocation , *COST estimates , *POPULATION dynamics - Abstract
There is interest in stock enhancement to support fisheries for California halibut Paralichthys californicus , a regionally important yet depleted species in the Southern California Bight (SCB), U.S. A quantitative model was developed to assess the cost of increasing harvestable California halibut in the SCB via releases of cultured juveniles. Reduced post-release survival of cultured juveniles was incorporated into the model, as was sex-specific lifetime survival, growth, and maturity. Simulations examined how adult California halibut population metrics were influenced by key release-strategy variables (i.e., size, number, survival, and sex ratio of released juveniles). A target increase in abundance of harvestable (≥ 559 mm total length; TL) California halibut by 5% in the entire SCB could likely be achieved by releasing large numbers (0.1–1.6 million per year) of larger-sized juveniles (40–200 mm TL). However, this was strongly mediated by their survival and sex ratio; fewer and smaller juveniles were required when releases comprised better surviving and / or more female individuals. Depending on the survival and sex ratio of released juveniles, increasing harvestable abundance in the SCB to achieve this 5% target was estimated to cost US$ 0.2–2.9 million annually, or US$ 14–219 per additional harvestable California halibut in the wild. This exploratory modeling study demonstrates that releasing juvenile California halibut could be a feasible management tool in the SCB, and provides a theoretical basis for field experiments. More broadly, it suggests that the allocation of resources to the production of larger and / or better surviving juveniles, in addition to optimizing release sex ratios, should be explored in stock enhancement programs. • Stock enhancement of California halibut was simulated by quantitative modeling. • Pre-defined target was achieved by releasing 0.1–1.6 million larger-sized (40–200 mm) juveniles. • Outcomes were strongly mediated by survival and sex ratio of released fish. • Annual costs to achieve the target were US$ 0.2–2.9 million. • Provides guidance for the allocation of resources in stock enhancement programs. [ABSTRACT FROM AUTHOR]
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- 2022
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28. Mechanism of microhomology-mediated end-joining promoted by human DNA polymerase θ.
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Kent, Tatiana, Chandramouly, Gurushankar, McDevitt, Shane Michael, Ozdemir, Ahmet Y, and Pomerantz, Richard T
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BASE pairs , *DNA polymerases , *POLYMERASE chain reaction , *HUMAN DNA , *NUCLEIC acids - Abstract
Microhomology-mediated end-joining (MMEJ) is an error-prone alternative double-strand break-repair pathway that uses sequence microhomology to recombine broken DNA. Although MMEJ has been implicated in cancer development, the mechanism of this pathway is unknown. We demonstrate that purified human DNA polymerase θ (Polθ) performs MMEJ of DNA containing 3′ single-strand DNA overhangs with ≥2 bp of homology, including DNA modeled after telomeres, and show that MMEJ is dependent on Polθ in human cells. Our data support a mechanism whereby Polθ facilitates end-joining and microhomology annealing, then uses the opposing overhang as a template in trans to stabilize the DNA synapse. Polθ exhibits a preference for DNA containing a 5′-terminal phosphate, similarly to polymerases involved in nonhomologous end-joining. Finally, we identify a conserved loop domain that is essential for MMEJ and higher-order structures of Polθ that probably promote DNA synapse formation. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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29. Sand-binding roots in Haemodoraceae: global survey and morphology in a phylogenetic context.
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Smith, Rhian, Hopper, Stephen, and Shane, Michael
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HAEMODORACEAE , *PLANT roots , *PLANT morphology , *PHYLOGENY , *PLANT health , *SOILS & nutrition - Abstract
Aims: To illustrate the morphology of sand-binding roots of Haemodoraceae, to conduct a comprehensive survey of the trait, spanning different climates across four continents, and to explore evolutionary hypotheses within a molecular phylogenetic framework. Methods: Sand-binding roots in Haemodoraceae were examined, measured and photographed in the field and on herbarium specimens. Photomicrographs were taken of southwest Australian species. The presence and absence of the sand-binding trait was mapped onto previously published phylogenies and an ancestral state reconstruction was performed. Results: Sand grains were very tightly bound to the root surface by persistent root hairs in Haemodoraceae. The majority of genera and species were found to possess sand-binding roots and only 2 of the 14 genera, Conostylis and Tribonanthes, had sister taxa with and without the trait. The trait was recorded in tropical, sub-tropical and wet temperate species, but mainly in semi-arid species. Sand-binding roots were likely to have been present in the ancestor of the family and both sub-families. Conclusions: The presence of sand-binding roots is the probable ancestral condition for Haemodoraceae, associated with a high degree of phylogenetic conservatism and some secondary loss, notably in Conostylis. Experimental studies are needed to understand the ecological and evolutionary forces at work. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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30. Acoustic characteristics of ultrasonic coded transmitters for fishery applications: Could marine mammals hear them?
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Bowles, Ann E., Denes, Samuel L., and Shane, Michael A.
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TRANSMITTERS (Communication) , *MARINE mammals , *UNDERWATER acoustic telemetry , *ACOUSTIC models , *ULTRASONICS - Abstract
Ultrasonic coded transmitters (UCTs) producing frequencies of 69-83 kHz are used increasingly to track fish and invertebrates in coastal and estuarine waters. To address concerns that they might be audible to marine mammals, acoustic properties of UCTs were measured off Mission Beach, San Diego, and at the U.S. Navy TRANSDEC facility. A regression model fitted to VEMCO UCT data yielded an estimated source level of 147 dB re 1 μPa SPL @ 1 m and spreading constant of 14.0. Based on TRANSDEC measurements, five VEMCO 69 kHz UCTs had source levels ranging from 146 to 149 dB re 1 μPa SPL @ 1 m. Five Sonotronics UCTs (69 kHz and 83 kHz) had source levels ranging from 129 to 137 dB re 1 μPa SPL @ 1 m. Transmitter directionality ranged from 3.9 to 18.2 dB. Based on propagation models and published data on marine mammal auditory psychophysics, harbor seals potentially could detect the VEMCO 69 kHz UCTs at ranges between 19 and >200 m, while odontocetes potentially could detect them at much greater ranges. California sea lions were not expected to detect any of the tested UCTs at useful ranges. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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31. Quantitative assessment of a white seabass (Atractoscion nobilis) stock enhancement program in California: Post-release dispersal, growth and survival
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Hervas, Susana, Lorenzen, Kai, Shane, Michael A., and Drawbridge, Mark A.
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QUANTITATIVE research , *WHITE seabass , *FISH populations , *ANIMAL dispersal , *FISH growth , *HATCHERY fishes , *FISH mortality - Abstract
Abstract: We evaluate post-release dispersal, growth and survival of hatchery-reared white seabass (Atractoscion nobilis) released as part of an experimental stock enhancement program in California. Releases of hatchery white seabass have been carried out since 1986, year round either directly from the hatchery or after acclimation in net pens. All released fish are implanted with coded wire tags. Post-release monitoring is carried out through a research gillnet fishery for juveniles, and tag returns from the commercial and recreational fisheries. Fish dispersed from the release sites at a diffusion rate D 795km2 year−1, such that 50% of fish remained within 47km and 95% within 135km of the release site at the end their third year at large. The von Bertalanffy growth parameters for released hatchery fish were estimated at L ∞ =975mm SL and K =0.21year−1. A set of alternative survival models accounting for dispersal and size- and time-dependent natural and fishing mortality patterns was confronted with the mark-recapture data using model selection based on the Akaike Information Criterion (AIC). The model that fit the data best accounted for short-term post-release mortality dependent on season and release method in addition to a long-term size-dependent mortality. Survival of released hatchery fish was highest in Spring, moderately lower in Summer and Autumn, but much lower in Winter releases. Acclimatisation in net pens had a substantial, positive effect on survival relative to direct releases. Survival of hatchery fish to legal minimum length (600mm SL) in the fishery was estimated at 1.5% for a release size of 200mm, rising to 13.8% for a release size of 400mm, under optimal conditions (Spring releases with net pen acclimatisation). Mortality rates of hatchery white seabass under optimal release conditions were substantially below average for other hatchery fish released into the wild, but remained above those expected for wild fish in both, the short-term and long-term components. [Copyright &y& Elsevier]
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- 2010
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32. Fisheries independent assessment of a returning fishery: Abundance of juvenile white seabass (Atractoscion nobilis) in the shallow nearshore waters of the Southern California Bight, 1995–2005
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Allen, Larry G., Pondella, Daniel J., and Shane, Michael A.
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FISHERIES , *WHITE seabass fisheries , *GILLNETTING - Abstract
Abstract: Nearshore, coastal and embayment areas off southern California were sampled to determine the spatial and temporal patterns abundance and size distributions of young white seabass in the shallow (5–14m) waters from Santa Barbara south to Imperial Beach off San Diego. A total of 19 stations, 13 in nearshore coastal waters and 6 in embayments, dispersed along the Southern California Bight were surveyed in each sampling month using 45.7m variable mesh, monofilament gill nets. In the 11-year period of sampling (April 1995–June 2005), a total of 8075 juvenile white seabass was captured in 42 sampling months. The mean catch-per-unit-effort (CPUE: 2.0fish/net±0.2) for juvenile white seabass varied significantly among stations during the 10-year period (1996–2005) of the full station sampling. Stations located near large rocky headlands, such as Palos Verdes, Santa Barbara, Newport, and La Jolla yielded the highest catches. Although CPUE peaked in August 1999 as a result of strong year classes in 1996–1998, overall, catches tripled over the 11-year sampling period at seven coastal sites increasing significantly at a rate equivalent to 0.22fish/(netyear). These relatively high catches of wild, juvenile fish over the last decade, along with significant increases in commercial CPUE and increased recreational catches overall, indicate that the natural population of white seabass is in the process of recovery. Commercial catches are again comparable to levels attained prior to the fishery collapse in the 1970s and 1980s. Therefore, we propose that the white seabass now represents one of the first documented cases of a recovering, demersal species of commercial importance. The ban of nearshore commercial gill net fishing by Proposition 132 probably contributed greatly to the increase in the population size that led to this recovery. In addition, the succession of warm water years that occurred from 1983 to the strong El Niño event of 1997–1998 also played an important role in the successful recruitment of white seabass. [Copyright &y& Elsevier]
- Published
- 2007
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33. PPARγ agonists do not directly enhance basal or insulin-stimulated Na+ transport via the epithelial Na+ channel.
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Nofziger, Charity, Chen, Lihong, Shane, Michael Anne, Smith, Chari D., Brown, Kathleen K., and Blazer-Yost, Bonnie L.
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PEROXISOMES , *INSULIN receptors , *DIABETES , *HORMONE receptors , *DRUG receptors , *VASOPRESSIN , *INSULIN , *ALDOSTERONE antagonists - Abstract
Selective agonists of peroxisome proliferator-activated receptor gamma (PPARγ) are anti-diabetic drugs that enhance cellular responsiveness to insulin. However, in some patients, fluid retention, plasma volume expansion, and edema have been observed. It is well established that insulin regulates Na+ reabsorption via the epithelial sodium channel (ENaC) located in the distal tubule. Therefore, we hypothesized that these agonists may positively modulate insulin-stimulated ENaC activity leading to increased Na+ reabsorption and fluid retention. Using electrophysiological techniques, dose–response curves for insulin-mediated Na+ transport in the A6, M-1, and mpkCCDcl4 cell lines were performed. Each line demonstrated hormone efficacy within physiological concentration ranges and, therefore, can be used to monitor clinically relevant effects of pharmacological agents which may affect electrolyte transport. Immunodetection and quantitative PCR analyses showed that each cell line expresses viable and functional PPARγ receptors. Despite this finding, two PPARγ agonists, pioglitazone and GW7845 did not directly enhance basal or insulin-stimulated Na+ flux via ENaC, as shown by electrophysiological methodologies. These studies provide important results, which eliminate insulin-mediated ENaC activation as a candidate mechanism underlying the fluid retention observed with PPARγ agonist use. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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34. Introduction.
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Lambers, Hans, Cramer, Michael D., Shane, Michael W., Wouterlood, Madeleine, Poot, Pieter, and Veneklaas, Erik J.
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PLANT-soil relationships , *PERIODICALS - Abstract
Presents an introductory statement to the January 2003 issue of the journal, "Plant and Soil."
- Published
- 2003
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35. No evidence of regulation in root-mediated iron reduction in two Strategy I cluster-rooted Banksia species (Proteaceae).
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Cawthray, Gregory R., Denton, Matthew D., Grusak, Michael A., Shane, Michael W., Veneklaas, Erik J., and Lambers, Hans
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PROTEACEAE , *CULTURE media (Biology) , *SPECIES , *IRON , *PHOSPHORUS , *PLANT roots - Abstract
Aims: Non-mycorrhizal species such as Banksia (Proteaceae) that depend on root exudates to acquire phosphorus (P) are prominent in south-western Australia, a biodiversity hotspot on severely P-impoverished soils. We investigated the consequences of an exudate-releasing P-mobilising strategy related to control of iron (Fe) acquisition in two Banksia species, B. attenuata R.Br. and B. laricina C. Gardner, that differ greatly in their geographical distribution and rarity. Methods: We undertook solution culture experiments to measure root-mediated Fe reduction (FeR) in non-cluster and cluster roots at four stages of cluster-root development, and whole root systems for plants grown at 2 to 300 μM Fe (as Fe-EDTA). As a positive control, we used Pisum sativum (cv. Dunn) to validate the FeR assay. Results: Unlike typical Strategy I species, both Banksia species showed no significant variation in FeR, for either cluster or non-cluster roots, when grown at a wide range of Fe supply. For roots of different developmental stages, we measured a range for B. attenuata cluster roots of 0.13 ± 0.03 to 1.29 ± 0.14 μmol Fe3+ reduced g−1 FW h−1 and 0.56 ± 0.11 to 1.10 ± 0.24 μmol Fe3+ reduced g−1 FW h−1 in non-cluster roots. Similarly, for B. laricina cluster-roots, FeR ranged from 0.22 ± 0.07 to 1.21 ± 0.37 μmol Fe3+ reduced g−1 FW h−1, and in non-cluster roots from 0.56 ± 0.11 to 0.71 ± 0.08 μmol Fe3+ reduced g−1 FW h−1. We also observed only minor differences for whole-root system FeR, and even though B. attenuata showed signs of leaf Fe deficiency in the 2 μM Fe treatment, its FeR was the lowest of both species across all treatments at 0.079 ± 0.009 μmol Fe3+ reduced g−1 FW h−1, compared with the fastest rate of 0.20 ± 0.014 μmol Fe3+ reduced g−1 FW h−1 for B. laricina in the 28 μM Fe treatment. Taking plants through a pulse from low to high Fe, then back to low Fe supply did not elucidate any significant response in FeR. Conclusions: Although Fe acquisition is tightly controlled in the investigated Banksia species, such control is not based on regulation of FeR, which challenges the model that is commonly accepted for Strategy I species. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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36. Leaf manganese concentrations as a tool to assess belowground plant functioning in phosphorus-impoverished environments.
- Author
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Lambers, Hans, Wright, Ian J., Guilherme Pereira, Caio, Bellingham, Peter J., Bentley, Lisa Patrick, Boonman, Alex, Cernusak, Lucas A., Foulds, William, Gleason, Sean M., Gray, Emma F., Hayes, Patrick E., Kooyman, Robert M., Malhi, Yadvinder, Richardson, Sarah J., Shane, Michael W., Staudinger, Christiana, Stock, William D., Swarts, Nigel D., Turner, Benjamin L., and Turner, John
- Subjects
- *
PLANT capacity , *FOLIAR diagnosis , *MANGANESE , *SOIL acidity , *WATER supply , *CARBOXYLATES , *RHIZOSPHERE - Abstract
Background and aims: Root-released carboxylates enhance the availability of manganese (Mn), which enters roots through transporters with low substrate specificity. Leaf Mn concentration ([Mn]) has been proposed as a signature for phosphorus (P)-mobilising carboxylates in the rhizosphere. Here we test whether leaf [Mn] provides a signature for root functional types related to P acquisition. Methods: Across 727 species at 66 sites in Australia and New Zealand, we measured leaf [Mn] as related to root functional type, while also considering soil and climate variables. To further assess the specific situations under which leaf [Mn] is a suitable proxy for rhizosphere carboxylate concentration, we studied leaf [Mn] along a strong gradient in water availability on one representative site. In addition, we focused on two systems where a species produced unexpected results. Results: Controlling for background site-specific variation in leaf [Mn] with soil pH and mean annual precipitation, we established that mycorrhizal species have significantly lower leaf [Mn] than non-mycorrhizal species with carboxylate-releasing root structures, e.g., cluster roots. In exception to the general tendency, leaf [Mn] did not provide information about root functional types under seasonally waterlogged conditions, which increase iron availability and thereby interfere with Mn-uptake capacity. Two further exceptions were scrutinised, leading to the conclusion that they were 'anomalous' in not functioning like typical species in their families, as expected according to the literature. Conclusions: Leaf [Mn] variation provides considerable insights on differences in belowground functioning among co-occurring species. Using this approach, we concluded that, within typical mycorrhizal families, some species actually depend on a carboxylate-releasing P-mobilising strategy. Likewise, within families that are known to produce carboxylate-releasing cluster roots, some do not produce functional cluster roots when mature. An analysis of leaf [Mn] can alert us to such 'anomalous' species. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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37. Femtosecond laser written diamond waveguide excitation of the whispering gallery modes in a silicon microsphere.
- Author
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Çirkinoğlu, Hüseyin Ozan, Bayer, Mustafa Mert, Serpengüzel, Ali, Rampini, Stefano, Sotillo, Belén, Bharadwaj, Vibhav, Ramponi, Roberta, and Eaton, Shane Michael
- Subjects
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WHISPERING gallery modes , *FEMTOSECOND lasers , *DIAMONDS , *QUALITY factor , *SILICON , *OPTICAL fibers - Abstract
We demonstrate light coupling from femtosecond laser written diamond waveguide operating at the telecommunication wavelengths to a silicon microsphere resonator. Type II waveguides are written on a diamond platform and Fabry-Pérot resonances are excited in the diamond waveguides by bare fiber coupling in and out of the waveguide. The Fabry-Pérot resonances have a free spectral range of 87 pm, and a maximum Q -factor of 3.9 × 104. The diamond waveguide is used to excite TM-polarized whispering gallery modes of the 1 mm silicon microsphere, since the type II waveguides support TM modes better as compared to TE modes. The whispering gallery mode spacing is measured as 0.257 nm and the highest Q -factor is 6.2 × 104. This evanescent coupling method to high Q -factor silicon microsphere resonances by femtosecond laser written diamond waveguides can be further used for filtering and sensing applications in various photonic lightwave circuits. • Shallow femtosecond laser-written Type IIa diamond waveguides can be used to couple light to spherical microresonators. • A 1 mm silicon microsphere coupled with the diamond waveguide exhibits high quality factor whispering-gallery-modes. • Fabry-Pérot resonances are triggered inside the diamond waveguide during end facet coupling from an optical fiber. • Microsphere resonator TM-modes exhibit better resonance performances when compared with the TE-modes. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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38. Opportunities for improving phosphorus-use efficiency in crop plants.
- Author
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Veneklaas, Erik J., Lambers, Hans, Bragg, Jason, Finnegan, Patrick M., Lovelock, Catherine E., Plaxton, William C., Price, Charles A., Scheible, Wolf-Rüdiger, Shane, Michael W., White, Philip J., and Raven, John A.
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CROPS , *CHEMICAL composition of plants , *PHOSPHORUS - Abstract
Contents [ABSTRACT FROM AUTHOR]
- Published
- 2012
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39. Hemodynamic Effects of Once-daily Tadalafil in Men With Signs and Symptoms of Benign Prostatic Hyperplasia on Concomitant α1-Adrenergic Antagonist Therapy: Results of a Multicenter Randomized, Double-Blind, Placebo-controlled Trial
- Author
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Goldfischer, Evan, Kowalczyk, John J., Clark, William R., Brady, Erin, Shane, Michael Anne, Dgetluck, Nancy, and Klise, Suzanne R.
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TADALAFIL , *HEMODYNAMICS , *BENIGN prostatic hyperplasia , *PLACEBOS , *CLINICAL pharmacology , *DOXAZOSIN , *ADRENERGIC beta agonists - Abstract
Objective: To investigate the safety of daily coadministration of α-blockers with tadalafil 5 mg in men with lower urinary tract symptoms secondary to benign prostatic hyperplasia. The standard-of-care medical therapy for moderate to severe symptoms of benign prostatic hyperplasia is α1-adrenergic antagonist (α-blocker) therapy. Methods: Men aged ≥ 45 years receiving stable α-blocker therapy were evaluated for eligibility before a 2-week single-blind, placebo lead-in period. Subsequently, 318 men were randomized to tadalafil 5 mg or placebo once daily for 12 weeks. Enrollment was monitored to ensure inclusion of men ≥75 years old and men taking nonuroselective α-blockers. The primary objective was to compare the proportion of men reporting treatment-emergent dizziness between the 2 treatment groups. Orthostatic vital signs, general safety, and the International Prostate Symptom Score were also assessed. Results: The proportion of patients who reported treatment-emergent dizziness was not significantly different between the 2 treatment groups (tadalafil 7.0%; placebo 5.7%; P = .403). No difference between treatment groups was observed with respect to patients meeting the criteria for a positive orthostatic test (30 per treatment group, P = 1.00). The incidence of discontinuations was low among both treatment groups. Conclusion: Recognizing the limitations of the present study, the changes in the hemodynamic signs and symptoms were similar for the tadalafil and placebo groups in men with benign prostatic hyperplasia receiving concomitant α-blocker therapy. However, consistent with the results of previous clinical pharmacology studies of healthy subjects, a trend was seen for increased hemodynamic signs and symptoms in men taking nonuroselective α-blockers, most notably those taking doxazosin. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
40. A Randomized, Double-Blind, Placebo-Controlled, Parallel Study to Assess the Efficacy and Safety of Once-A-Day Tadalafil in Men with Erectile Dysfunction Who Are Naïve to PDE5 Inhibitors.
- Author
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Montorsi, Francesco, Aversa, Antonio, Moncada, Ignacio, Perimenis, Petros, Porst, Hartmut, Barker, Clare, Shane, Michael A., and Sorsaburu, Sebastian
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CLINICAL trials , *IMPOTENCE , *TREATMENT of sexual dysfunction , *CLINICAL drug trials , *PHOSPHODIESTERASE inhibitors , *DISEASES in men - Abstract
ABSTRACT Introduction. The majority of subjects included in previous tadalafil once-a-day clinical trials were non-naïve to previous phosphodiesterase 5 (PDE5) inhibitors on demand. A study on PDE5 inhibitor naïve subjects was therefore warranted. Aim. To evaluate the efficacy and safety of once-a-day tadalafil in PDE5 inhibitor-naïve men with erectile dysfunction (ED). Main Outcomes Measures. Primary efficacy end points were changes from baseline to end point in the International Index of Erectile Function (IIEF) Erectile Function (EF) domain score and the per-subject proportion of 'yes' responses to sexual encounter profile (SEP) question 2 (SEP2) and question 3 (SEP3). Methods. PDE5 inhibitor-naïve men with ED (N = 217) were randomized in a 1:2 ratio to receive placebo or tadalafil 5 mg once a day for 12 weeks. Enrollment began in January 2009 and the last subject completed in January 2010. Results. At end point, least square mean change from baseline IIEF-EF domain score (7.3 vs. 3.4), SEP2 (23.8% vs. 12.2%) and SEP3 (39.5% vs. 21.5%), was significantly larger for tadalafil vs. placebo (all P < 0.001). The most common adverse events (AEs) in tadalafil-treated subjects were back pain, nasopharyngitis, dyspepsia, headache, and myalgia. Four subjects (2.7%) in the tadalafil group and one subject (1.4%) in the placebo group discontinued because of AEs. Conclusions. In PDE5 inhibitor-naïve men, tadalafil once a day significantly improved EF compared with placebo. Safety results were consistent with previous tadalafil once-a-day clinical trials. Montorsi F, Aversa A, Moncada I, Perimenis P, Porst H, Barker C, Shane MA, and Sorsaburu S. A randomized, double-blind, placebo-controlled, parallel study to assess the efficacy and safety of once-a-day tadalafil in men with erectile dysfunction who are naïve to PDE5 inhibitors. J Sex Med 2011;8:2617-2624. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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41. Phosphorus Nutrition of Proteaceae in Severely Phosphorus-Impoverished Soils: Are There Lessons To Be Learned for Future Crops?
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Lambers, Hans, Finnegan, Patrick M., Lalibert, Etienne, Pearse, Stuart J., Ryan, Megan H., Shane, Michael W., and Veneklaas, Erik J.
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PLANT-soil relationships , *CROPS & soils , *SOILS , *SOIL management , *ARABLE land - Abstract
The article discusses the traits in Proteaceae from soils with extremely low phosphorous (P) availability and evaluates whether the traits are desirable for crops. It points out that the capacity to form root clusters and mycorrhizal associations are adaptive traits that improve the ability of plants to acquire soil. It cites available strategies to improve P efficiency in cropping systems such as making greater use of crop species with cluster roots, exploring the potential of native species and understanding the molecular basis of P-efficient traits.
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- 2011
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42. METTL13 Methylation of eEF1A Increases Translational Output to Promote Tumorigenesis.
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Liu, Shuo, Hausmann, Simone, Carlson, Scott Moore, Fuentes, Mary Esmeralda, Francis, Joel William, Pillai, Renjitha, Lofgren, Shane Michael, Hulea, Laura, Tandoc, Kristofferson, Lu, Jiuwei, Li, Ami, Nguyen, Nicholas Dang, Caporicci, Marcello, Kim, Michael Paul, Maitra, Anirban, Wang, Huamin, Wistuba, Ignacio Ivan, Porco, John Anthony, Bassik, Michael Cory, and Elias, Joshua Eric
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NEOPLASTIC cell transformation , *METHYLATION , *LUNG cancer , *XENOGRAFTS , *HOMOGRAFTS - Abstract
Summary Increased protein synthesis plays an etiologic role in diverse cancers. Here, we demonstrate that METTL13 (methyltransferase-like 13) dimethylation of eEF1A (eukaryotic elongation factor 1A) lysine 55 (eEF1AK55me2) is utilized by Ras-driven cancers to increase translational output and promote tumorigenesis in vivo. METTL13-catalyzed eEF1A methylation increases eEF1A's intrinsic GTPase activity in vitro and protein production in cells. METTL13 and eEF1AK55me2 levels are upregulated in cancer and negatively correlate with pancreatic and lung cancer patient survival. METTL13 deletion and eEF1AK55me2 loss dramatically reduce Ras-driven neoplastic growth in mouse models and in patient-derived xenografts (PDXs) from primary pancreatic and lung tumors. Finally, METTL13 depletion renders PDX tumors hypersensitive to drugs that target growth-signaling pathways. Together, our work uncovers a mechanism by which lethal cancers become dependent on the METTL13-eEF1AK55me2 axis to meet their elevated protein synthesis requirement and suggests that METTL13 inhibition may constitute a targetable vulnerability of tumors driven by aberrant Ras signaling. Graphical Abstract Highlights • METTL13 is the physiologic eEF1A lysine 55 dimethyltransferase • METTL13 dimethylation of eEF1A stimulates protein synthesis in cancer cells • The METTL13-eEF1A methylation axis fuels Ras-driven tumorigenesis in vivo • METTL13 depletion sensitizes cancer cells to PI3K and mTOR pathway inhibitors Ras-driven cancers ramp up protein synthesis by increasing the GTPase activity of a translation elongation factor through a mechanism that involves METTL13-catalyzed eEF1A dimethylation [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
43. Diamond photonics platform enabled by femtosecond laser writing.
- Author
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Sotillo, Belén, Bharadwaj, Vibhav, Hadden, J. P., Sakakura, Masaaki, Chiappini, Andrea, Fernandez, Toney Teddy, Longhi, Stefano, Jedrkiewicz, Ottavia, Shimotsuma, Yasuhiko, Criante, Luigino, Osellame, Roberto, Galzerano, Gianluca, Ferrari, Maurizio, Miura, Kiyotaka, Ramponi, Roberta, Barclay, Paul E., and Eaton, Shane Michael
- Published
- 2016
- Full Text
- View/download PDF
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