1. Magnetic-stirring-enhanced mechanical amorphous dispersion extraction for the hydrophobic phytochemical constituents using an aqueous solution from a medicinal plant.
- Author
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Jin, Huang-Fei, Shen, Qian-Xue, Shi, Ying, Liu, Fang-Ming, Wang, Bin, Cao, Jun, and Ye, Li-Hong
- Subjects
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AQUEOUS solutions , *RESPONSE surfaces (Statistics) , *MEDICINAL plants , *DISPERSION (Chemistry) , *ANTHRAQUINONES - Abstract
A method involving chitosan-assisted magnetic-stirring-enhanced mechanical amorphous dispersion extraction was developed and utilized to extract hydrophobic anthraquinones from Rhei Radix et Rhizoma prior to ultrahigh performance liquid chromatography analysis. Incorporating natural chitosan as a dispersant facilitated the extraction of hydrophobic anthraquinones using purified water, considerably enhancing the eco-friendliness of the extraction methodology. To optimize extraction efficiency, an extensive evaluation of the crucial parameters influencing rhubarb yield was conducted. Furthermore, a response surface methodology was applied to optimize the extraction conditions. Under these optimized conditions, the method exhibited linearity ranges of 0.1–100 µg/mL, with correlation coefficients between 0.9990 and 0.9998. The method's intraday (n = 6) and interday (n = 6) precision levels were maintained at ≤3.58%, which was considered to be within acceptable limits. The computed detection and quantification limits were 16.54–24.60 and 54.91–82.04 ng/mL, respectively. Consequently, this optimized method was effectively employed to extract five specific compounds (aloe-emodin, emodin, rhein, chrysophanol, and physcion) from Rhei Radix et Rhizoma, achieving recoveries ranging from 86.43% to 102.75%. [Display omitted] • Chitosan-assisted mechanical amorphous dispersion extraction method was developed. • The method shows good practicability for anthraquinone extraction. • Response surface methodology was employed to investigate the extraction conditions. • Water was used as extraction solvent with no environmental pollution. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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