1. Structure Characterization and Anti-tumor Activity of Polysaccharides from Rohdea chinensis.
- Author
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Shihao Zhang, Minghua Ma, Sicheng Liu, Guoce Jin, Wencong Li, Yu Zhao, Yanyu Li, Xueyan Li, Zixuan Zhu, Chengxiong Liu, Zhaoxia Liu, Xiaocong Li, and Kun Zou
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INULIN , *POLYSACCHARIDES , *ANTINEOPLASTIC agents , *FRUCTANS , *HOT water , *BODY weight , *MOLECULAR weights - Abstract
Three crude polysaccharides of RCP-A, B, and C were derived from the rhizome of Rohdea chinensis by means of hot water extraction, gradient ethanol precipitation and dialysis. Three different polysaccharides of RCP-C1-1, RCP-C1-2, and RCP-C1-3 were isolated using cellulose DEAE-52 and Sephadex G-200 chromatography from RCP-C. The average molecular weights of RCP-C1-1, RCP-C1-2, and RCP-C1-3 were measured as 1.51×104, 1.06×104, and 4.86×10³ by means of MALDI-TOF MS and UHPGC, respectively. All three polysaccharides were found to consist of D-fructose and D-glucose following hydrolysis and comparison with literature data. Based on FT-IR and NMR analysis, the polysaccharides were identified as inulin-type fructans, with their backbone composed of α-D-glucopyranosyl-(1→2)-(β-D-fructofuranosyl)n-(1→2)-β-Dfructofuranoside (nRCP-C1-1=79, nRCP-C1-2=58, nRCP-C1-3=27). The anti-tumor activity of the polysaccharides (RCPA, B, and C) was evaluated in H22 tumor-bearing mice. The results suggested that the polysaccharides (RCP-A, RCP-B, and RCP-C) inhibited the growth of H22 hepatocellular. Further, the treated groups of RCP-A, RCP-B, and RCP-C exhibited improvements in body weight as well as spleen/thymus indexes in H22 tumor-bearing mice. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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