Search

At present, epigenetic markers have been extensively studied in various fields and have a high value in forensic medicine due to their unique mode of inheritance, which does not involve DNA sequence alterations. As an epigenetic phenomenon that plays an important role in gene expression, non-coding RNAs (ncRNAs) act as key factors mediating gene silencing, participating in cell division, and regulating immune response and other important biological processes. With the development of molecular biology, genetics, bioinformatics, and next-generation sequencing (NGS) technology, ncRNAs such as microRNA (miRNA), circular RNA (circRNA), long non-coding RNA (lncRNA), and P-element induced wimpy testis (PIWI)-interacting RNA (piRNA) are increasingly been shown to have potential in the practice of forensic medicine. NcRNAs, mainly miRNA, may provide new strategies and methods for the identification of tissues and body fluids, cause-of-death analysis, time-related estimation, age estimation, and the identification of monozygotic twins. In this review, we describe the research progress and application status of ncRNAs, mainly miRNA, and other ncRNAs such as circRNA, lncRNA, and piRNA, in forensic practice, including the identification of tissues and body fluids, cause-of-death analysis, time-related estimation, age estimation, and the identification of monozygotic twins. The close links between ncRNAs and forensic medicine are presented, and their research values and application prospects in forensic medicine are also discussed. [ABSTRACT FROM AUTHOR]

Han is the largest of China's 56 ethnic groups and the most populous ethnic group in the world. The Luzhou region is located in southwest China, at the junction of three provinces. The unique historical factors contribute to the genetic polymorphism information. Short tandem repeats (STRs) are highly polymorphic, but the polymorphism of the Y chromosomal STRs (Y-STRs) loci in the Luzhou region is still unclear. It is of great significance to provide Y-STRs genetic data for the Han population from the Luzhou areas of southwest China. A total of 910 unrelated male individuals of the Han population from the Luzhou area were recruited, and 24 Y-STRs were analyzed. The population structure and phylogenetic relationships were compared with those of another 11 related Han populations. A total of 893 different haplotypes were achieved from 910 samples, of which 877 (98.21%) haplotypes were unique. Haplotype diversity and discrimination were 0.999956 and 0.981319, respectively. The lowest genetic diversity of DYS437 is 0.4321, and the highest genetic diversity of DYS385a/b is 0.9642. Pair-to-pair genetic distance and relative probability values indicate that Luzhou Han people are close to Sichuan Han people, Guangdong Han people, and Hunan Han people, which is consistent with geographical distribution, historical influence, and economic development. The 24 Y-STR markers of the southwest Luzhou Han population were highly polymorphic, which provided us with genetic polymorphism information and enriched the population genetic database. Therefore, it is of great value to our forensic applications and population genetics research. [ABSTRACT FROM AUTHOR]

The Han nationality is an ancient and populous people, and different places in China may have their distinct group relationships. Luzhou area, as a crossroads of several provinces in Southwest China, lacks autosomal short tandem repeat (STR) research and population genetics research. In this study, 21 autosomal STR loci were evaluated in 1959 Han-Chinese individuals from Luzhou area. There was no substantial linkage disequilibrium (LD) among the 21 autosomal STR markers, and all markers were in Hardy–Weinberg equilibrium (HWE). The total discrimination power (TDP) and cumulative probability of exclusion (CPE) of the 21 autosomal STR loci were calculated to be 1–9.8505 × 10−16 and 1–1.9406 × 10−9, respectively. There were 333 alleles for 21 STRs with allelic frequencies ranging from 0.00026 to 0.51302, and the number of alleles ranged from 7 in locus TPOX to 29 in locus Penta E. According to the results of population comparison and population differentiation, historical influences, geographical distribution, cultural integration, and economic development may have an impact on the Luzhou Han population and other Chinese populations. These 21 STR loci were found to enrich autosomal STR information in forensic databases and provide highly informative polymorphisms for our forensic practice in China, including personal identification and parentage testing. [ABSTRACT FROM AUTHOR]

The Yi is one of fifty-six ethnic populations and one of the most ancient ethnic groups in China. The Liangshan Yi Autonomous Prefecture (LYAP) in Sichuan Province has the single largest Yi community in China. To establish a Yi population database in the LYAP of Sichuan in China, a Goldeneye™ DNA Identification System 20A Kit with 19 autosomal STRs (short tandem repeats) was used. As a result, the total discrimination power (TDP) and the cumulative probability of exclusion (CPE) for these STRs in 1016 unrelated individuals were 0.999999999999999999999897 and 0.9999999597, respectively. Totals of 273 alleles for 19 STRs and 8–22 alleles for each locus were found. The allelic frequencies ranged from 0.0005 to 0.5084. The forensic parameter averages of these STRs were as follows: observed heterozygosity (Hobs) of 78.44%, expected heterozygosity (Hexp) of 79.89%, discrimination power (DP) of 92.66%, and probability of exclusion (PE) of 57.68%. Penta E presented the highest levels of Hobs and DP, whereas TPOX showed the lowest Hobs and DP values. Nei's standard genetic distance matrix among 31 populations found that the nearest genetic distance to the Yi population was the Sichuan Han (0.0056). Altogether, we first reported the forensic parameters and allele frequencies of 19 autosomal STRs of the Yi group in Liangshan. These 19 STR makers could provide highly informative polymorphisms for individual identification, paternity testing and genetic population analyses. [ABSTRACT FROM AUTHOR]

To clarify the sliding mechanism of a landslide, the shear behavior characteristics of slip zone soil are always vital. In the Northwest of China, thousands of landslides generally develop in three main types of slip zone soil, i.e., Quaternary loess, weathered Mesozoic-Cenozoic mudstone, and weathered Mesozoic-Paleozoic phyllite. In the present study, the shear behavior characteristics of these three kinds of slip zone soil were investigated through direct shear tests and their implications for the sliding mechanisms of landslide were also discussed. It was found that, in general, an abrupt failure of loess landslide could be related to the strong strain-softening behavior of loess in the direct shear test, while the ductile shear behavior of weathered mudstone and phyllite coincided with the creep deformation of the corresponding landslides. The residual friction angle of slip zone soil varied with the soil type and its mineral composition, and the lowest residual friction angle about 11.5° could be found in weathered phyllite (SET) with graphite. Compared with the previous research, it is interesting to find that the residual friction angles of mudstone and phyllite in the present study accorded reasonably with the empirical correlations proposed by Wen et al. (2007) for weak rocks in the Three Gorges area, China. [ABSTRACT FROM AUTHOR]

The point load strength test index (Is(50)) rapidly determines the tensile strength and the uniaxial compressive strength of rocks. It is necessary to analyze the power index (m) of Is(50) when conducting a point load test (PLT) on irregular rock blocks. In this study, sandy dolomites from the Sinian Dengying Formation (Zbdn) and Doushantuo Formation (Zbd) in the Yuxi section of the Central Yunnan Water Diversion Project were analyzed for their physical and mechanical properties. PLTs were performed on dolomites with different geological origins and weathering degrees, and the m-values were determined through statistical methods and regression analysis. The results showed that the logP–logDe2 curve exhibits a negative slope, and the m-value and its correlation coefficient (R2) increased with the decreasing weathering degree. For similar weathering conditions, the R2 of the Zbdn dolomites was higher than that of the Zbd dolomites. The error analysis indicated that the obtained m-values were highly accurate for the dolomite with different geological origins and weathering degrees. Casting slice identification tests further confirmed that the dolomite pore structure and density, connectedness, and specific mineral composition were the primary factors influencing the m-value. Highlights: This is the first study on the power index (m) of sandy dolomites. The logP–logDe2 curve exhibits a negative slope, and m-value and its R2 increase with decreasing weathering degree. Pore structure and density, connectedness, and specific mineral composition primarily influence the m-value. For a similar weathering degree, the R2 of Zbdn dolomites was higher than that of Zbd dolomites. [ABSTRACT FROM AUTHOR]

This study examines the small-strain stress-strain properties during cyclic loading of Lanzhou loess deposits, characterized by a very high void ratio (>0.9). Torsional resonant column (RC) tests are performed in order to investigate the effect of water content, confining pressure, consolidation time and soil structure strength on the small-strain stiffness ( G 0 ) and damping (D min ) of loess. It is proved that shear modulus G 0 at low strain levels is very sensitive to water content while the effect of water content on small-strain damping D min is relatively small. As water content W approaches the plastic limit PL, there is a dramatic decrease of G 0 compared to drier loess specimen; G 0 has the tendency to reach a stable or constant value with further increase of water content which is more than the PL of loess. The small strain damping D min decreases continuously with water content for unsaturated loess and then tends to increase slightly for almost fully saturated loess. A linear relationship between G 0 and confining pressure σ′ m in log-log coordinates for different water contents is observed for Lanzhou loess. Similarly, D min decreases linearly with σ′ m . A power function is proposed to describe the relationship between G 0 and confining pressure σ′ m where the exponent m varies with the water content of loess. G 0 is also affected by the consolidation duration and a power function is proposed between normalized G 0 and normalized consolidation time. G 0 is finally strongly affected by the structure of loess and the type of specimen. For undisturbed loess samples, G 0 is always higher than reconstituted ones and the difference decreases with the degree of saturation; for fully saturated samples the influence of the structure strength between undisturbed and reconstituted samples becomes insignificant. Likewise, D min of undisturbed loess is higher than that for remolded loess under the same confining pressure and the difference between them reduces with the degree of saturation as well. [ABSTRACT FROM AUTHOR]

Loess is a kind of world-recognized problematic soil and, as a consequence, it is a major hazard in geotechnical engineering. This study examines the small to medium strain dynamic properties of Lanzhou intact and recompacted loess through a set of resonant column (RC) tests. The influence of soil structure on the small-strain shear modulus G 0 , the variations of G / G 0, and damping ratio DT(%) with shear strain γ were analyzed and discussed. The test results show that the structure has an important influence on the dynamic properties of dry loess at small and medium strain, whereas, with the increase of moisture, its effect weakens. It is proved that a normalized correlation between G / G 0 and γ/γ 0.7 is practically identical for undisturbed and recompacted loess in Lanzhou, irrespective of soil structure, which may be useful in practical applications. • The small to medium strain dynamic properties of Lanzhou loess with metastable structure were examined. • Structure effect reduces the ductility in dynamic response for Lanzhou loess. • The correlations between G / G 0 and γ/γ 0.70 could be normalized well for Lanzhou loess, irrespective of structure effect. [ABSTRACT FROM AUTHOR]

To investigate the impact of permeability and heterogeneity on oil displacement efficiency and remaining oil distribution of chemical flooding, three groups of high and ultrahigh permeability core samples from an ultrahigh water-cut oilfield in western China were selected as the research objects in this study. High-pressure mercury injection, scanning electron microscopy, wettability test, and other methods were used to characterize the reservoir properties of core samples. Six groups of experiments were performed using the nuclear magnetic resonance (NMR) displacement imaging technology to simulate the oilfield development process considering the economic benefits. The displacement stage with the best oil displacement effect in the process of waterflooding, chemical flooding and then waterflooding was defined, and the control effect of permeability and heterogeneity on the improvement of oil displacement efficiency by polymer-surfactant binary flooding was discussed. The distribution position of remaining oil in different displacement stages was quantitatively and visually displayed, and its control factors were revealed. The research shows that during the simulation process of first waterflooding followed by chemical flooding and then waterflooding in the oilfield, the T2 spectrum signal amplitude increases the most in the two stages, one is from saturated oil flooding to 50% water cut and the other one is from 95% water cut to the end of 1 PV polymer flooding. The oil displacement efficiency increases the most, and the oil is primarily discharged from pore throats larger than 90 ms (or with pore throat radius of 8.37 μm). Compared with heterogeneity, permeability plays a more obvious controlling role in improving the oil displacement efficiency of polymer-surfactant binary flooding. The influence of fingering phenomenon on the distribution of remaining oil is most obvious in the second waterflooding, and the distribution of remaining oil with polymer slug is more obviously affected by the fingering phenomenon than that with polymer-surfactant slug. The study results provide theoretical guidance for tapping the remaining oil potential of old oilfields with high to ultrahigh permeabilities. [ABSTRACT FROM AUTHOR]

To investigate the impact of permeability and heterogeneity on oil displacement efficiency and remaining oil distribution of chemical flooding, three groups of high and ultrahigh permeability core samples from an ultrahigh water-cut oilfield in western China were selected as the research objects in this study. High-pressure mercury injection, scanning electron microscopy, wettability test, and other methods were used to characterize the reservoir properties of core samples. Six groups of experiments were performed using the nuclear magnetic resonance (NMR) displacement imaging technology to simulate the oilfield development process considering the economic benefits. The displacement stage with the best oil displacement effect in the process of waterflooding, chemical flooding and then waterflooding was defined, and the control effect of permeability and heterogeneity on the improvement of oil displacement efficiency by polymer-surfactant binary flooding was discussed. The distribution position of remaining oil in different displacement stages was quantitatively and visually displayed, and its control factors were revealed. The research shows that during the simulation process of first waterflooding followed by chemical flooding and then waterflooding in the oilfield, the T2 spectrum signal amplitude increases the most in the two stages, one is from saturated oil flooding to 50% water cut and the other one is from 95% water cut to the end of 1 PV polymer flooding. The oil displacement efficiency increases the most, and the oil is primarily discharged from pore throats larger than 90 ms (or with pore throat radius of 8.37 μm). Compared with heterogeneity, permeability plays a more obvious controlling role in improving the oil displacement efficiency of polymer-surfactant binary flooding. The influence of fingering phenomenon on the distribution of remaining oil is most obvious in the second waterflooding, and the distribution of remaining oil with polymer slug is more obviously affected by the fingering phenomenon than that with polymer-surfactant slug. The study results provide theoretical guidance for tapping the remaining oil potential of old oilfields with high to ultrahigh permeabilities. [ABSTRACT FROM AUTHOR]

Dysregulation of long non-coding RNA (IncRNA) antisense non-coding RNA in the INK4 locus (ANRIL) is associated with the risk of myocardial infarction (MI). Therefore, the present study aimed to determine the mechanisms underlying this association, which is currently poorly understood, to the best of our knowledge. The current study used an in vitro myocardial ischemia and reperfusion (MI/R) model, in which H9c2 cardiomyocytes were exposed to hypoxia/reoxygenation (H/R), which demonstrated that ANRIL expression was downregulated and that ANRIL positively regulated sirtuin 1 (SIRT1) expression following H/R injury. Subsequently, it was demonstrated that ANRIL upregulated SIRT1 expression by sponging microRNA-181a (miR-181a). In addition, ANRIL overexpression reduced lactate dehydrogenase release and apoptosis of H9c2 cardiomyocytes exposed to H/R, indicating that ANRIL prevented H/R-induced cardiomyocyte injury. Moreover, both miR-181a overexpression and SIRT1 knockdown significantly decreased the protective effects of ANRIL on H/R-induced cardiomyocyte injury, thus demonstrating that SIRT1 upregulation via sponging miR-181a is a critical mechanism that mediates the function of ANRIL. These results provided a novel mechanistic insight into the role of ANRIL in H/R-injured cardiomyocytes and suggested that the ANRIL/miR-181a/SIRT1 axis may be a therapeutic target for reducing MI/R injury. [ABSTRACT FROM AUTHOR]

Re-compaction process after previous liquefaction exerts significant effects on subsequent cyclic behaviour of soil. Due to the complexities in environmental loading conditions, the relevant studies on this topic are limited. In this paper, we collected natural submarine silts from non-liquefied and post-liquefied seabed in the Yellow River subaqueous delta via offshore hydraulic sampling method, respectively. Monotonic and cyclic triaxial tests were conducted between them to investigate the re-compaction effect after previous wave-induced liquefaction on the undrained static and cyclic shear behaviours of seabed soil. Our experiments indicated that due to re-compaction process, the static and cyclic strengths of seabed silt in post-liquefied area are both enhanced in comparison to non-liquefied soil. A transformation in static shear failure mode from plastic to brittle after wave-induced liquefaction could be identified in seabed silt. Furthermore, the developments of pore water pressure and cyclic axial strain with number of cycles for seabed silts could be characterized by the hyperbolic and power models, respectively, and an empirical relationship between pore water pressure and cyclic axial strain is proposed for natural seabed silt. Finally, compared with non-liquefied seabed silt, the threshold cyclic stress ratio (CSR) for post-liquefied seabed silt is also elevated due to re-compaction effect. • The post-liquefaction re-compaction effect on cyclic behaviour of natural seabed silty soils was clarified. • After post-liquefaction re-compaction, the static shear strength of seabed silt was enhanced. • After post-liquefaction re-compaction, the liquefaction resistance of seabed silt was improved. [ABSTRACT FROM AUTHOR]

The sheep lice eggshells and felting are both important problem in wool industry. Deep eutectic solvents (DES) are a class of eco-friendly and low-cost solvents that are used in various fields including the anti-felting of wool but not in the eggshell removal. In this study, the simultaneous removal of wool scales and lice eggshells using [ChCl:Urea] was achieved by optimizing the formula and condition. Satisfactory eggshell removal (80.0%) and anti-felting rates (22.7%) had little effect on the mechanical strength of the wool (12.1%). The mechanism of the beneficial effects was studied using scanning electron microscopy and Fourier-transform infrared spectroscopy. The loose structure of protein cement is conducive to the penetration of DESs to achieve complete disintegration and dissolution. In contrast, the dense wool structure prevents DESs from penetrating the interior and only removes the surface scales. In addition, dissolution of eggshells by DES also helps in its removal. To our knowledge, this is the first report of DES-mediated one-step eggshell removal and anti-felting of wool. Through economic and environmental effects analyses, DES-based technique exhibits a more promising potential for industrial applications. This work not only has significant application prospects in the wool industry, but also the application scenarios of DESs. [Display omitted] [ABSTRACT FROM AUTHOR]

The sheep lice eggshells and felting are both important problem in wool industry. Deep eutectic solvents (DES) are a class of eco-friendly and low-cost solvents that are used in various fields including the anti-felting of wool but not in the eggshell removal. In this study, the simultaneous removal of wool scales and lice eggshells using [ChCl:Urea] was achieved by optimizing the formula and condition. Satisfactory eggshell removal (80.0%) and anti-felting rates (22.7%) had little effect on the mechanical strength of the wool (12.1%). The mechanism of the beneficial effects was studied using scanning electron microscopy and Fourier-transform infrared spectroscopy. The loose structure of protein cement is conducive to the penetration of DESs to achieve complete disintegration and dissolution. In contrast, the dense wool structure prevents DESs from penetrating the interior and only removes the surface scales. In addition, dissolution of eggshells by DES also helps in its removal. To our knowledge, this is the first report of DES-mediated one-step eggshell removal and anti-felting of wool. Through economic and environmental effects analyses, DES-based technique exhibits a more promising potential for industrial applications. This work not only has significant application prospects in the wool industry, but also the application scenarios of DESs. [Display omitted] [ABSTRACT FROM AUTHOR]

As a cellular protease, transmembrane serine protease 2 (TMPRSS2) plays roles in various physiological and pathological processes, including cancer and viral entry, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Herein, we conducted expression, mutation, and prognostic analyses for the TMPRSS2 gene in pan-cancers as well as in COVID-19-infected lung tissues. The results indicate that TMPRSS2 expression was highest in prostate cancer. A high expression of TMPRSS2 was significantly associated with a short overall survival in breast invasive carcinoma (BRCA), sarcoma (SARC), and uveal melanoma (UVM), while a low expression of TMPRSS2 was significantly associated with a short overall survival in lung adenocarcinoma (LUAD), demonstrating TMPRSS2 roles in cancer patient susceptibility and severity. Additionally, TMPRSS2 expression in COVID-19-infected lung tissues was significantly reduced compared to healthy lung tissues, indicating that a low TMPRSS2 expression may result in COVID-19 severity and death. Importantly, TMPRSS2 mutation frequency was significantly higher in prostate adenocarcinoma (PRAD), and the mutant TMPRSS2 pan-cancer group was significantly associated with long overall, progression-free, disease-specific, and disease-free survival rates compared to the wild-type (WT) TMPRSS2 pan-cancer group, demonstrating loss of functional roles due to mutation. Cancer cell lines were treated with small molecules, including cordycepin (CD), adenosine (AD), thymoquinone (TQ), and TQFL12, to mediate TMPRSS2 expression. Notably, CD, AD, TQ, and TQFL12 inhibited TMPRSS2 expression in cancer cell lines, including the PC3 prostate cancer cell line, implying a therapeutic role for preventing COVID-19 in cancer patients. Together, these findings are the first to demonstrate that small molecules, such as CD, AD, TQ, and TQFL12, inhibit TMPRSS2 expression, providing novel therapeutic strategies for preventing COVID-19 and cancers. [ABSTRACT FROM AUTHOR]

[Display omitted] • A pH- & redox-responsive system was developed for CPT delivery. • CPT@Zein-FA improves the bioactivity of CPT against lepidopteran pests. • CPT@Zein-FA has baiting properties that accelerate the death of pests. Pesticides are critical to securing crop yields, but the extremely low utilization of conventional pesticides puts a serious strain on human development and the environment. Natural product pesticides meet the needs of sustainable development, but challenges like high costs and slow efficacy hinder their widespread use. Camptothecin (CPT) is a natural product with potent bioactivity for various insect pests, but its bioactivity for lepidopteran pests is limited due to the ring-opening in their alkaline midgut. To enhance CPT's efficacy, a nanopesticide CPT@Zein-FA, which consists of the plant-derived zein and ferulic acid (FA), was designed and verified to be able to improve the bioactivity of CPT against lepidopteran pests including Ostrinia furnacalis , Spodoptera frugiperda and Mythimna separata. The nanopesticide CPT@Zein-FA approximately 100 nm in size, exhibited uniform dispersion, strong adhesion, and stability. CPT@Zein-FA utilizes pH and redox dual response mechanisms to achieve protection in the midgut environment and release in the hemocoel/intracellular environment. With the help of zein bait, CPT@Zein-FA can also promote the feeding of insects and accelerate their death. Metabolomic analysis revealed CPT's lethal impact on insect metabolism by inhibiting nucleotide synthesis, disrupting body size and development through lipid metabolism and insect hormone synthesis inhibition. This work provides new ideas for the large-scale application of natural product pesticides and contributes to the sustainable development of the world. [ABSTRACT FROM AUTHOR]