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Abstract   Nowadays microfabrication techniques originating from micro-electro nics enable to create mechanical objects of micron-size. The field of Micro-Electro-Mechanical devices (MEMs) is continuously expanding, with an amazingly broad range of applications at room temperature. Vibrating objects (torsional oscillators, vibrating wires) widely used at low temperatures to study quantum fluids, can be replaced advantageously by Silicon MEMs. In this letter we report on the study of Silicon vibrating wire devices. A goal-post structure covered with a metal layer is driven at resonance by the Laplace force acting on a current in a magnetic field, while the induced voltage arising from the cut magnetic flux allows to detect the motion. The characteristics of the resonance have been studied from 10 mK to 30 K, in vacuum and in 4He gas. In this article, we focus on the results obtained above 1.5 K, in vacuum and gas, and introduce some features observed at lower temperatures. The resonant properties can be quantitatively understood by means of simple models, from the linear regime to a highly non-linear response at strong drives. We demonstrate that the non-linearity is mostly due to the geometry of the vibrators. We also show that in our device the friction mechanisms originate in the metallic layers, and can be fully characterized. The interaction with 4He gas is fit to theory without adjustable parameters. [ABSTRACT FROM AUTHOR]

Plasma concentrations of biologically active vitamin D (1,25- (OH)2D) are tightly controlled via feedback regulation of renal la-hydroxylase (CYP27B1; positive) and 24-hydroxylase (CYP24AJ; catabolic) enzymes. In pregnancy, this regulation is uncoupled, and 1,25-(OH)2D levels are significantly elevated, suggesting a role in pregnancy progression. Epigenetic regulation of CYP27BJ and CYP24AJ has previously been described in cell and animal models, and despite emerging evidence for a critical role of epigenetics in placentation generally, little is known about the regulation of enzymes modulating vitamin D homeostasis at the fetomaternal interface. In this study, we investigated the methylation status of genes regulating vitamin D bioavailability and activity in the placenta. No methylation of the VDR (vitamin D receptor) and CYP27B1 genes was found in any placental tissues. In contrast, the CYP24A1 gene is methylated in human placenta, purified cytotrophoblasts, and primary and cultured chorionic villus sampling tissue. No methylation was detected in any somatic human tissue tested. Methylation was also evident in marmoset and mouse placental tissue. All three genes were hypermethylated in choriocarcinoma cell lines, highlighting the role of vitamin D deregulation in this cancer. Gene expression analysis confirmed a reduced capacity for CYP24A1 induction with promoter methylation in primary cells and in vitro reporter analysis demonstrated that promoter methylation directly down-regulates basal promoter activity and abolishes vitamin D-mediated feedback activation. This study strongly suggests that epigenetic decoupling of vitamin D feedback catabolism plays an important role in maximizing active vitamin D bioavailability at the fetomaternal interface. [ABSTRACT FROM AUTHOR]

Abstract  We report on the dynamic measurements of thermal properties of nanosystems at very low temperatures. These techniques are based on the modulation of the temperature and hence leads to highly sensitive measurements. We will discuss the intrinsic limitations of these methods when the thermal properties of nano-objects are studied at very low temperatures, much below 1 K. Firstly, we will present thermal conductance measurements using the 3ω method. This technique is limited at low temperatures due to the significant increase of the mean free path. Secondly, heat capacity measurements using ac calorimetry are outlined, and again restrictions occur due to the continuous temperature gradient inherent to that technique. Propositions are made in order to overcome these limitations. [ABSTRACT FROM AUTHOR]