1. Utility of a GFP-expressing Barley yellow mosaic virus for analyzing disease resistance genes.
- Author
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Mai Tanokami, Wei Qin Wang, Meimi Yamamoto, Tomomi Hagiwara, Mari Yumoto, Aika Tomiyama, Satoru Mine, Yukiko Tamura, Shunichi Kobayashi, Yoshiko Nakazawa, Tsuneo Kato, Tomohide Natsuaki, and Hisashi Nishigawa
- Subjects
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MOSAIC viruses , *VIRUS diseases , *DISEASE resistance of plants , *PHYTOPLASMAS , *GREEN fluorescent protein , *BARLEY yellow dwarf viruses - Abstract
The soil-borne plasmodiophorid Polymyxa graminis is a vector for Barley yellow mosaic virus (BaYMV), which can severely damage barley plants. Although 22 disease resistance genes have been identified, only a few have been used for breeding virus-resistant cultivars. Recently, BaYMV strains capable of overcoming the effects of some of these genes have been detected. In this study, green fluorescent protein (GFP)-expressing BaYMV was constructed and used to examine viral dynamics in inoculated barley plants. Leaf inoculations resulted in higher infection rates than root or crown inoculations. Additionally, inoculations of some resistant cultivars produced infections that were similar to those observed in a field test. The results of this study indicate that the GFP-expressing virus is a useful tool for visualizing virus replication and dynamics, and for understanding resistance mechanisms. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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