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Background: Posterior cervical fusion (PCF) with lateral mass screws is a favorable treatment option to revise a symptomatic pseudarthrosis due to reliable rates of arthrodesis; however, this technique introduces elevated risk for wound infection and hospital readmission. A tissue-sparing PCF approach involving facet fixation instrumentation reduces the rates of postoperative complications while stabilizing the symptomatic level to achieve arthrodesis; however, these outcomes have been limited to small study cohorts from individual surgeons commonly with mixed indications for treatment. Materials and Methods: One hundred and fifty cases were identified from a retrospective chart review performed by seven surgeons across six sites in the United States. All cases involved PCF revision for a pseudarthrosis at one or more levels from C3 to C7 following anterior cervical discectomy and fusion (ACDF). PCF was performed using a tissue-sparing technique with facet instrumentation. Cases involving additional supplemental fixation such as lateral mass screws, rods, wires, or other hardware were excluded. Demographics, operative notes, postoperative complications, hospital readmission, and subsequent surgical interventions were summarized as an entire cohort and according to the following risk factors: age, sex, number of levels revised, body mass index (BMI), and history of nicotine use. Results: The average age of patients at the time of PCF revision was 55 ± 11 years and 63% were female. The average BMI was 29 ± 6 kg/m2 and 19% reported a history of nicotine use. Postoperative follow-up visits were available with a median of 68 days (interquartile range = 41-209 days) from revision PCF. There were 91 1-level, 49 2-level, 8 3-level, and 2 4±-level PCF revision cases. The mean operative duration was 52 ± 3 min with an estimated blood loss of 14 ± 1.5cc. Participants were discharged an average of 1 ± 0.05 days following surgery. Multilevel treatment resulted in longer procedure times (single = 45 min, multi = 59 min, P = 0.01) but did not impact estimated blood loss (P = 0.94). Total nights in the hospital increased by 0.2 nights with multilevel treatment (P = 0.01). Sex, age, nicotine history, and BMI had no effect on recorded perioperative outcomes. There was one instance of rehospitalization due to deep-vein thrombosis, one instance of persistent pseudarthrosis at the revised level treated with ACDF, and four instances of adjacent segment disease. In patients initially treated with multilevel ACDF, revisions occurred most commonly on the caudal level (48% of revised levels), followed by the cranial (43%), and least often in the middle level (9%). Conclusions: This chart review of perioperative and safety outcomes provides evidence in support of tissue-sparing PCF with facet instrumentation as a treatment for symptomatic pseudarthrosis after ACDF. The most common locations requiring revision were the caudal and cranial levels. Operative duration and estimated blood loss were favorable when compared to open alternatives. There were no instances of postoperative wound infection, and the majority of patients were discharged the day following surgery. [ABSTRACT FROM AUTHOR]

A human-in-the-loop simulation experiment was conducted at the NASA Langley Research Center's Air Traffic Operations Laboratory in an effort to comprehensively validate tools and procedures intended to enable the small aircraft transportation system higher-volume-operations concept of operations. These procedures were developed with the goal of increasing the rate of operations at nontowered, nonradar airports in near all-weather conditions. A key element of the new design is the establishment of a volume of airspace around designated airports at which pilots accept responsibility for self-separation. Flights operating at these airports are given approach-sequencing information computed by a ground-based automated system. The validation experiment was conducted to determine if a pilot could safely and proficiently fly an airplane while performing the newly developed procedures. Comparative measures of flight path error, perceived workload, and situation awareness were obtained for two types of simulated scenarios. Baseline scenarios were representative of today's system using procedure separation, in which air traffic control grants one approach or departure clearance at a time. Test scenarios represented the newly developed approach and departure procedures. Results from the experiment indicate that low-time pilots were able to fly the test procedures in a simulation environment and maintain self-separation as safely and proficiently as flying today's procedures. [ABSTRACT FROM AUTHOR]

Paroxysmal hypnogenic dyskinesia is a rare clinical entity characterized by intermittent dystonia and choreoathetoid movements that begin exclusively during sleep, often with consciousness preserved once the patient is awakened during the episodes. They occur almost every night and are often misdiagnosed as sleeping disorders. Paroxysmal hypnogenic dyskinesia is currently known to be a form of frontal lobe epilepsy, but not in all cases. We present a 19-year-old male patient with paroxysmal hypnogenic dyskinesia who responded to antihistamines. This supports an alternative theory from 1977 (before the cases had been adequately described) that the disorder lies in dysregulation in the basal ganglia. This description now appears similar to acute dystonic reactions such as extrapyramidal symptoms from antipsychotic medications, which also respond to antihistamines. [ABSTRACT FROM AUTHOR]

Deals with the use of protein kinase chips to evaluate the protein kinases encoded by the yeast genome. Examination of kinases ability to phosphorylate using the chips; Processes involved in the assays of protein kinase activity; Information on how the protein chip technology works; Discussion on other techniques to assess protein kinase function.

Examines the proton demand inversion in a mutant protein tyrosine kinase reaction. Mechanism of phosphoryl transfer; Occurrence of nucleophile-phosphorus bond formation; Catalytic loop of protein kinase.

This paper summarizes findings from the Small Aircraft Transportation System Higher Volume Operations Flight Experiment. The higher volume operations concept improves efficiency at nontowered, nonradar airports in instrument meteorological conditions. The success of the higher volume operations concept is based on pilot acceptability as determined through objective and subjective assessments when compared with the procedural control operations in use today at nontowered, nonradar-controlled airfields in instrument meteorological conditions. Flight experiment data indicate that the concept is viable. The experiment, flown on a general aviation aircraft, used a subset of the Higher Volume Operations Simulation Experiment scenarios and evaluation pilots to validate the simulation experiment results. Results reveal that all 12 low-time instrument-rated pilots preferred Small Aircraft Transportation System Higher Volume Operations when compared with current procedural separation operations. These pilots also flew the higher volume operations procedures safely and proficiently without additional workload in comparison to today's system. Detailed results of pilot flight technical error and their subjective assessments of workload and situation awareness are presented. [ABSTRACT FROM AUTHOR]

A multilayer approach to the prevention of conflicts due to the toss of aircraft-to-aircraft separation that relies on procedures and onboard automation was implemented as part of the Small Aircraft Transportation Systems Higher-Volume-Operations concept. The multitayer system gives pilots support and guidance during the execution of normal operations and advance warning for procedure deviations or off-nominal operations. This paper describes the major concept elements of this multilayer approach to separation assurance and conflict prevention and provides the rationale for its design. All the algorithms and functionality described in this paper were implemented in an aircraft simulation in the Air Traffic Operations Laboratory at NASA Langley Research Center and on the NASA Cirrus SR22 research aircraft. [ABSTRACT FROM AUTHOR]

Abstract: One objective of the Small Aircraft Transportation System (SATS) Project is to increase the capacity and utilization of small non-towered, non-radar equipped airports by transferring traffic management activities to an automated system and separation responsibilities to general aviation (GA) pilots. This paper describes the development of a research multi-function display (MFD) to support the interaction between pilots and an automated Airport Management Module (AMM). Preliminary results of simulation and flight tests indicate that adding the responsibility of monitoring other traffic for self-separation does not increase pilots’ subjective workload levels. Pilots preferred using the enhanced MFD to execute flight procedures, reporting improved situation awareness (SA) over conventional instrument flight rules (IFR) procedures. [Copyright &y& Elsevier]

Abstract: The RNA polymerase holoenzyme is a proven target for antibacterial agents. A high-throughput screening program based on this enzyme from Staphylococcus aureus had previously identified a 2-ureidothiophene-3-carboxylate as a low micromolar inhibitor. An investigation of the relationships between the structures of this class of compounds and their inhibitory- and antibacterial activities is described here, leading to a set of potent RNA polymerase inhibitors with antibacterial activity. Characterization of this bioactivity, including studies of the mechanism of action, is provided, highlighting the power of the reverse chemical genetics approach in providing tools to inhibit the bacterial RNA polymerase. [Copyright &y& Elsevier]

Heterotrimeric G proteins can signal to reorganize the actin cytoskeleton, but the mechanism is unclear. Here we report that, in tyrosine kinase Csk-deficient mouse embryonic fibroblast cells, G protein (Gβγ, Gα12, Gα13, and Gαq)-induced, and G protein-coupled receptor-induced, actin stress fiber formation was completely blocked. Reintroduction of Csk into Csk-deficent cells restored the G protein-induced actin stress fiber formation. Chemical rescue experiments with catalytic mutants of Csk demonstrated that the catalytic activity of Csk was required for this process. Furthermore, we uncovered that Gβγ can both translocate Csk to the plasma membrane and directly increase Csk kinase activity. Our genetic and biochemical studies demonstrate that Csk plays a critical role in mediating G protein signals to actin cytoskeletal reorganization. [Copyright &y& Elsevier]