1. Discriminating Astragali Radix from Its Adulterants Using HPLC Coupled with Chemometric Clustering Techniques.
- Author
-
WEI-WEI DONG, AU, DAWN, XIN-WEI CAO, XIAO-BO LI, and DA-JIAN YANG
- Subjects
- *
ASTRAGALUS membranaceus , *CLUSTER analysis (Statistics) , *FACTOR analysis , *FLAVONOIDS , *FOOD chemistry , *HERBAL medicine , *HIGH performance liquid chromatography , *CHINESE medicine , *REGRESSION analysis , *RESEARCH funding , *DATA analysis software , *DESCRIPTIVE statistics - Abstract
A simple high-performance liquid chromatography (HPLC) method coupled with principal component analysis (PCA) and hierarchical cluster analysis (HCA) was developed for distinguishing Astragali Radix from its adulterants. Five species, including A. membranaceus var. mongolicus, A. membranaceus, H. polybotrys, A. chrysopterus and A. ernestii, were analyzed by this method. At the same time, four major bioactive isoflavonoids, namely calycosin-7-O-D-glucopyranoside, ononin, calycosin and formononetin, were simultaneously determined in Astragali Radix. This method was proven to have good precision, repeatability, stability and recovery. It was successfully applied to distinguish Astragali Radix from its adulterants species and can be used for the quality control of Astragali Radix [ABSTRACT FROM AUTHOR]
- Published
- 2011