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Objective: To analyze the acupoint selection rules for cheek acupuncture in the treatment of chronic abdominal pain by data mining technology. Methods: The chronic abdominal pain medical records of Professor Ouyang Bowen in the outpatient clinic of Guangdong Provincial Hospital of Traditional Chinese Medicine from January 2019 to December 2022 were used as the data source. The medical record data (basic information, Western medicine diagnosis, Chinese medicine diagnosis, Chinese medicine syndrome type, physical examination target, acupoint prescription, VAS score results) were entered into Excel2019 software to establish a database and standardize the processing. The frequency of acupoint use was descriptively analyzed by Excel 2019; the SPSS Modeler 18.0 Apriori algorithm was used to analyze the association rules of the prescriptions and mine the frequent item sets of acupoints; the SPSS 26.0 software was used to cluster the high-frequency acupoints, draw the pedigree diagram, and mine the clustering relationship between acupoints. Results: After strict screening according to the inclusion and exclusion criteria, a total of 133 medical records, 133 prescriptions, 21 acupoints involved, and a total frequency of 863 times were obtained. The top five acupoints in terms of frequency of use are back acupoints, cervical acupoints, lumbar acupoints, sacral acupoints, and abdominal plexus acupoints. Among the acupoint combinations, cervical acupoints, lumbar acupoints, and back acupoints have the strongest correlation; acupoints used more than 10 times can be divided into three effective clusters. Conclusion: Professor Ouyang Bowen treats chronic abdominal pain by regulating the autonomic nerves, focusing on the referred pain caused by visceral nerve sensitivity, and mainly uses spinal acupoints such as cervical acupoints, back acupoints, lumbar acupoints, and sacral acupoints. [ABSTRACT FROM AUTHOR]

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) has long been studied from many perspectives. As a multisubunit (large subunits [LSUs] and small subunits[SSUs]) protein encoded by genes residing in the chloroplast (rbcL) and nuclear (rbcS) genomes, RuBisCo also is a model for cytonuclear coevolution following allopolyploid speciation in plants. Here, we studied the genomic and transcriptional cytonuclear coordination of auxiliary chaperonin and chaperones that facilitate RuBisCo biogenesis across multiple natural and artificially synthesized plant allopolyploids. We found similar genomic and transcriptional cytonuclear responses, including respective paternal-to-maternal conversions and maternal homeologous biased expression, in chaperonin/chaperon-assisted folding and assembly of RuBisCo in different allopolyploids. One observation is about the temporally attenuated genomic and transcriptional cytonuclear evolutionary responses during early folding and later assembly process of RuBisCo biogenesis, which were established by long-term evolution and immediate onset of allopolyploidy, respectively. Our study not only points to the potential widespread and hitherto unrecognized features of cytonuclear evolution but also bears implications for the structural interaction interface between LSU and Cpn60 chaperonin and the functioning stage of the Raf2 chaperone. [ABSTRACT FROM AUTHOR]

Recombination between homeologous chromosomes, also known as homeologous exchange (HE), plays a significant role in shaping genome structure and gene expression in interspecific hybrids and allopolyploids of several plant species. However, the molecular mechanisms that govern HEs are not well understood. Here, we studied HE events in the progeny of a nascent allotetraploid (genome AADD) derived from two diploid progenitors of hexaploid bread wheat using cytological and whole-genome sequence analyses. In total, 37 HEs were identified and HE junctions were mapped precisely. HEs exhibit typical patterns of homologous recombination hotspots, being biased toward low-copy, subtelomeric regions of chromosome arms and showing association with known recombination hotspot motifs. But, strikingly, while homologous recombination preferentially takes place upstream and downstream of coding regions, HEs are highly enriched within gene bodies, giving rise to novel recombinant transcripts, which in turn are predicted to generate new protein fusion variants. To test whether this is a widespread phenomenon, a dataset of highresolution HE junctions was analyzed for allopolyploid Brassica, rice, Arabidopsis suecica, banana, and peanut. Intragenic recombination and formation of chimeric genes was detected in HEs of all species and was prominent in most of them. HE thus provides a mechanism for evolutionary novelty in transcript and protein sequences in nascent allopolyploids. [ABSTRACT FROM AUTHOR]