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We explore the properties of stellar kinematics and ionized gas in a sample of 1106 local (U)LIRGs from the AKARI telescope. We combine data from Wide-field Infrared Survey Explorer and Sloan Digital Sky Survey Data Release 13 to fit the spectral energy distribution (SED) of each source to constrain the contribution of active galactic nuclei (AGNs) to the total IR luminosity and estimate physical parameters such as stellar mass and star formation rate (SFR). We split our sample into AGNs and weak/non-AGNs. We find that our sample is considerably above the main sequence. The highest SFRs and stellar masses are associated with ULIRGs. We also fit the H β and H α regions to characterize the outflows. We find that the incidence of ionized gas outflows in AGN (U)LIRGs (∼72%) is much higher than that in weak/non-AGN ones (∼39%). The AGN ULIRGs have extreme outflow velocities (up to ∼2300 km s−1) and high mass-outflow rates (up to ∼60 M ⊙ yr−1). Our results suggest that starbursts are insufficient to produce such powerful outflows. We explore the correlations of SFR and specific SFR (sSFR) with ionized gas outflows. We find that AGN hosts with the highest SFRs exhibit a negative correlation between outflow velocity and sSFR. Therefore, in AGNs containing large amounts of gas, the negative feedback scenario might be suggested. [ABSTRACT FROM AUTHOR]

We investigate the ionized gas kinematics relationship with X-ray, radio and accreting properties using a sample of 348 nearby ( z < 0.4 ) SDSS-FIRST-X-ray detected AGNs. X-ray properties of our sample are obtained from XMM-Newton, Swift and Chandra observations. We unveil the ionized gas outflows in our sample manifested by the non-gravitational broad component in [O iii]λ5007Å emission line profiles. From the comparison of the correlation of non-parametric outflow velocities (i.e., the velocity width, the maximal velocity of outflow and line dispersion) with X-ray luminosity and radio luminosity, we find that outflow velocities have similarly positive correlations with both X-ray and radio luminosity. After correcting for the gravitational component, we find that the [O iii] velocity dispersion normalized by stellar mass also increases with both X-ray luminosity and radio luminosity. We also find that, for a given X-ray (radio) luminosity, radio (X-ray) luminous AGNs have higher outflow velocities than non-radio (non-X-ray) luminous AGNs. Therefore, we find no clear preference between X-ray luminosity and radio luminosity in driving high-velocity ionized outflows and conclude that both AGN activity and small-scale jets contribute comparably. Moreover, there is no evidence that our obscured AGNs are preferentially associated with higher velocity outflows. Finally, we find a turning point around log (λ E d d) ≃ − 1.3 when we explore the dependency of outflow velocity on Eddington ratio. It can be interpreted considering the role of high radiation pressure (log (λ E d d) ≳ − 1.3 ) in causing drastic reduction in the covering factor of the circumnuclear materials. [ABSTRACT FROM AUTHOR]

By using a large, highly obscured () active galactic nucleus (AGN) sample (294 sources at z ∼ 0–5) selected from detailed X-ray spectral analyses in the deepest Chandra surveys, we explore distributions of these X-ray sources in various optical/infrared/X-ray color–color diagrams and their host-galaxy properties, aiming at characterizing the nuclear obscuration environment and the triggering mechanism of highly obscured AGNs. We find that the refined Infrared Array Camera (IRAC) color–color diagram fails to identify the majority of X-ray-selected, highly obscured AGNs, even for the most luminous sources with. Over 80% of our sources will not be selected as heavily obscured candidates using the flux ratio of and R − K > 4.5 criteria, implying complex origins and conditions for the obscuring materials that are responsible for the heavy X-ray obscuration. The average star formation rate (SFR) of highly obscured AGNs is similar to that of stellar mass- (M*-) and z-controlled normal galaxies, while a lack of quiescent hosts is observed for the former. Partial correlation analyses imply that highly obscured AGN activity (traced by) appears to be more fundamentally related to M*, and no dependence of on either M* or SFR is detected. Morphology analyses reveal that 61% of our sources have a significant disk component, while only ∼27% of them exhibit irregular morphological signatures. These findings together point toward a scenario where secular processes (e.g., galactic-disk instabilities), instead of mergers, are most probable to be the leading mechanism that triggers accretion activities of X-ray-selected, highly obscured AGNs. [ABSTRACT FROM AUTHOR]

The rest-frame UV/optical variability of the quasars in the Sloan Digital Sky Survey Stripe 82 is used to test the Corona-Heated Accretion-disk Reprocessing (CHAR) model of Sun et al. We adopt our CHAR model and the observed black hole masses (MBH) and luminosities (L) to generate mock light curves that share the same measurement noise and sampling as the real observations. Without any fine-tuning, our CHAR model can satisfactorily reproduce the observed ensemble structure functions for different MBH, L, and rest-frame wavelengths. Our analyses reveal that a luminosity-dependent bolometric correction is disfavored over the constant bolometric correction for UV/optical luminosities. Our work demonstrates the possibility of extracting quasar properties (e.g., the bolometric correction or the dimensionless viscosity parameter) by comparing the physical CHAR model with quasar light curves. [ABSTRACT FROM AUTHOR]

We present a detailed X-ray spectral analysis of 1152 active galactic nuclei (AGNs) selected in the Chandra Deep Fields (CDFs), in order to identify highly obscured AGNs (>). By fitting spectra with physical models, 436 (38%) sources with are confirmed to be highly obscured, including 102 Compton-thick (CT) candidates. We propose a new hardness ratio measure of the obscuration level that can be used to select highly obscured AGN candidates. The completeness and accuracy of applying this method to our AGNs are 88% and 80%, respectively. The observed log N−log S relation favors cosmic X-ray background models that predict moderate (i.e., between optimistic and pessimistic) CT number counts. Nineteen percent (6/31) of our highly obscured AGNs that have optical classifications are labeled as broad-line AGNs, suggesting that, at least for part of the AGN population, the heavy X-ray obscuration is largely a line-of-sight effect, i.e., some high column density clouds on various scales (but not necessarily a dust-enshrouded torus) along our sight line may obscure the compact X-ray emitter. After correcting for several observational biases, we obtain the intrinsic distribution and its evolution. The CT/highly obscured fraction is roughly 52% and is consistent with no evident redshift evolution. We also perform long-term (≈17 yr in the observed frame) variability analyses for 31 sources with the largest number of counts available. Among them, 17 sources show flux variabilities: 31% (5/17) are caused by the change of , 53% (9/17) are caused by the intrinsic luminosity variability, 6% (1/17) are driven by both effects, and 2 are not classified owing to large spectral fitting errors. [ABSTRACT FROM AUTHOR]

Abstract: Here, a new acute myelomonocytic leukemia (AMML) cell line, JIH-3, is reported, and its biological characteristics are described. JIH-3 cells were maintained without any cytokines for 27 months. The JIH-3 cell line showed typical myelomonocytic morphological features. Additionally, it mainly expressed myeloid and monocytic markers (CD13, CD14, CD11b, CD15 and CD33), although it also expressed other antigens such as the markers of T and B lymphocytic lineage as well as stem cell, progenitor cell, and natural killer cell-related antigens (CD4, CD5, CD7, CD10, CD22, CD34, CD38, HLADR, CD16/CD56 and CD56); the expression of these markers, suggested that this cell line was in the early stage of myelomonocytic differentiation. Cytogenetic analysis initially showed a karyotype of 46, XY, del(7) (p1?3p2?2). During the passage period, the cells with this karyotype gradually decreased and were replaced by cells with a 45,XY,dic(4;7)(p11;p11),del(15)(q2?2) karyotype. Chromosome painting showed a deletion in the short arm of chromosome 7 for del(7)(p1?3p2?2) and der(4;7)(p11;p11). The latter had larger deleted segment than the former. Fluorescence in situ hybridization (FISH) revealed the dicentric nature of der(4;7), and Multiplex FISH (M-FISH) confirmed that der(4;7) was an unbalanced translocation. A deletion involving the 7p region on dic(4;7)(p11;p11) harbors many genes, including CDC2L5, C7ORF11, C7ORF10 and INHBA. Haploinsufficiency of the genes on 4p, 7p and 15q caused by deletions of 4p, 7p and 15q2?2 that resulted from dic(4;7)(p11;p11) and del(15)(q2?2) may play important roles in leukemogenesis and in the establishment of the JIH-3 cell line. JIH-3 cells did not express multidrug resistance (MDR)-related genes and apoptosis-related genes such as MDR1, multidrug resistance-related protein, lung resistance protein, BCL-2, Bax, GS-π or Fax, only P21 expression was detected, which probably leads the MDR indirectly through inhibition of the activities of cyclin-dependent kinase (CDK). JIH-3 cells had tumorigenic capacity in nude mice. In conclusion, JIH-3 is a new acute myelomonocytic leukemia cell line. It is from a well-characterized background and provides a new useful tool for the study of leukemia patients with a 7p deletion. [Copyright &y& Elsevier]

Abstract: Fluorescence in situ hybridization (FISH) is being used increasingly in cytogenetic diagnosis of myelodysplastic syndromes (MDS). However, the utility of FISH in this role has not been well-defined. A total of 249 de novo MDS patients were submitted to karyotyping and FISH analysis for −5/del(5)(q31), −7/del(7)(q31), +8, −17/i(17)(q10), del(20)(q12), and −Y. Of the 234 patients with available karyotypic data, 143 cases (60.9%) demonstrated normal karyotype and 91 cases (39.1%) showed abnormal karyotype. FISH confirmed R-banding findings in 96.6% (226/234) of samples with successful karyotyping and detected cytogenetic abnormalities in 46.7% (7/15) of cases with karyotype failure. Of the 3.4% (8/234) patients showing discrepancies between FISH and R-banding, FISH revealed cytogenetic abnormalities in four patients with normal karyotypes and four patients with complex karyotypes. These results highlight FISH analysis has limited value in MDS cases with successful karyotyping and is only informative in MDS cases with karyotype failure. [Copyright &y& Elsevier]

Abstract: A pericentric inv(9)(p22q34) of the derivative chromosome 9 that resulted from a standard t(9;22)(q34;q11.2) was identified by R-banding karyotypic analysis and fluorescence in situ hybridization (FISH) assays in 4 (0.18%) of 2,200 Philadelphia chromosome (Ph)-positive leukemia patients, including 3 with chronic myeloid leukemia (CML) in chronic phase and 1 with acute myeloid leukemia (AML) in our hospital since 2004. All four patients had two malignant clones: one with only t(9;22)(q34;q11.2) and another with der(9)t(9;22)(q34;q11.2)inv(9)(p22q34) that resulted in the separation of the ABL1/BCR fusion gene. No metaphases with only inv(9)(p22q34) were seen in any of them. FISH also found a deletion of partial sequence of BCR on der(9)t(9;22)(q34;q11.2)inv(9)(p22q34) in 67.5% of bone marrow cells in the AML patient, but did not detect the deletion of the sequence of ASS/9q34 in these four patients. Reverse transcriptase–polymerase chain reaction revealed a b3a2 type of BCR/ABL1 fusion transcript in all of them, proving their disease to be Ph-positive leukemia. On reviewing the literature, only two solitary Ph-positive leukemia patients have been noticed to have the inv(9)(p22q34) anomaly. These two patients, together with our four documented patients, indicate that inv(9)(p22q34) is a novel, rare, but recurrent secondary chromosomal abnormality for Ph-positive leukemia. Despite receiving hydroxyurea therapy (n = 3 patients), combined chemotherapy (n = 2), even imatinib treatment (n = 1), three patients, including one with AML and two with CML (one of whom progressed into the lymphoblastic blast phase), died with survival times of 28 days, 13 months, and 34 months, respectively. Only one patient with CML remained alive for 5.5 months. Their negative outcome implies that inv(9)(p22q34) has an unfavorable impact on prognosis. Presently, no firm conclusions can be drawn from this study. Because the case number reported here is very small, more patients with this anomaly need to be investigated to elucidate its true prognostic significance. [Copyright &y& Elsevier]

Abstract: To date, at least 25 translocations involving the NUP98 gene and different partner genes have been reported in the literature. Here, we describe a novel reciprocal t(5;11)(q31;p15) involving NUP98, as revealed by conventional karyotypic analysis using R-banding technique and fluorescence in situ hybridization (FISH) using a BAC RP11-120E20 probe and whole chromosome paint probes for chromosomes 5 and 11 in a 77-year-old woman who was diagnosed as having de novo acute myeloid leukemia. The patient received two courses of intensive combined chemotherapy but did not reach complete remission. She eventually died from the progressive disease, surviving for only 1 month after diagnosis. FISH analysis using WCP5 together with BAC RP11-878F9 or RP11-155N22 demonstrated that the breakpoint of chromosome 5 is located on 5q31. In addition, the EGR1 gene was unexpectedly found to be lost in the FISH study using EGR1 (red)/D5S23, D5S721 (green) dual-color probe. We supposed that the fusion gene created by t(5;11)(q31;p15) consisting of the NUP98 and its partner gene, as well as the loss of the EGR1 gene, may play a cooperative role in leukemogenesis. The partner gene of NUP98 in t(5;11)(q31;p15) is unclear at this time. Further molecular study is required to identify this partner gene in our patient. [Copyright &y& Elsevier]

Abstract: Translocation (14;14)(q11;q32) is one of the recurrent chromosome aberrations in ataxia-teleangiectasia (AT) and T-cell malignancies. In patients with the t(14;14), the TCL1 and TCRα/δ genes were found to be involved at the molecular level. However, t(14;14)(q11;q32) is an exceedingly rare phenomenon in B-lineage acute lymphoblastic leukemia (B-ALL). To date, it has been reported in only 5 B-ALL cases. Here, we report another B-ALL case with t(14;14)(q11;q32) in a 39-year-old female. The immunophenotype of the blasts showed positivity for CD79a, CD10, CD19, and HLA-DR. Chromosome analysis of the bone marrow (BM) cells at presentation showed the karyotype 47,XX,+4,t(14;14)(q11;q32). Fluorescence in situ hybridization (FISH) demonstrated trisomy 4 and the simultaneous involvement of the IGH gene at 14q32 and the CEBPE gene at 14q11, which differs from the genes involved in T-cell leukemias. After chemotherapy, the patient achieved complete remission (CR). Later, she received allogeneic peripheral blood stem cell transplantation. After CR, the karyotype of the BM cells was normal. She was disease-free at a 6-month follow-up. We suggest that t(14;14)(q11;q32) involving the IGH and CEBPE genes in B-ALL is rare, but it is a recurrent abnormality that could identify a new subgroup of B-ALL. [Copyright &y& Elsevier]

Objective: To report here a new acute myelocytic leukemia (AML) cell line SH-2 and describe its biological characteristics. Materials and Methods: Mononuclear cells isolated from a patient with AML-M2 subtype were passaged by liquid culture medium. Interleukin-3 and bone marrow stromal cells were used to support cell proliferation at the first 3 months. Various methods, including cytogenetic analysis, fluorescence in situ hybridization (FISH), multiplex FISH (M-FISH), reverse transcriptase polymerase chain reaction (RT-PCR), multiplex RT-PCR, short tandem repeat (STR)–PCR, direct sequencing of DNA, clonogenic assay, and tumorigenicity in nude and severe combined immunodeficient (SCID) mice were employed to identify and characterize SH-2 cell line. Results: SH-2 cells were maintained without cytokine and stromal cells for 3 years. It had no Epstein-Barr virus or mycoplasma contamination. The SH-2 cell line showed typical myelocytic features in morphology and simultaneous strongly expressed myeloid antigens (CD13, 99.6% and CD33, 99.26%) and natural killer (NK)–related antigens (CD56, 99.5% and CD16/56, 99.62%) suggesting that SH-2 is an AML cell line with NK-antigen expression. SH-2 cell line initially showed a karyotype of 45, X, -Y, der(16)t(16;17)(q24;q12), −17, +19. During the passage period, the cells with a hypodiploid karyotype gradually decreased and were replaced by the near-tetraploid cells with a karyotype of 71-105(86), XX, -Y, -Y, der(16)t(16;17)×2, −17, −17, +19, +19. FISH and M-FISH delineated all abnormalities. SH-2 cells had the approximately same morphological, immunophenotypical, and cytogenetic features as the patient''s leukemia cells had. STR-PCR provided powerful evidence for the derivation of SH-2 cell line from the patient''s leukemia cells. SH-2 cells showed multiple drug resistance (MDR), which may be related to the p53 gene alteration, including the loss of one p53 allele due to the monosomy 17 and a point mutation of CAG to CAT at codon 576 of exon 5 in another p53 allele resulting in the loss of p53 gene function. In addition, SH-2 cell line did not express MDR-related genes, such as MDR1, multidrug resistance-related protein, and lung resistance protein, but expressed apoptosis-related genes, such as Bcl-2, Fas, glutathione S-transferase-π, and p21, which were also related to the MDR. SH-2 cell line had tumorigenic capacities in nude and SCID mice. Conclusion: Because SH-2 cell line had a clear biology background, it will provide a useful tool for the study of the pathogenesis and treatment strategy of AML with MDR. [Copyright &y& Elsevier]

Abstract: The cytogenetic anomaly der(20)del(20)(q11.2q13.3)idic(20)(p11), or idic(20q−) in short form, has been reported in 13 cases of myelodysplastic syndrome, one case of chronic myelomonocytic leukemia, and one case of acute myeloid leukemia since 2004. To our knowledge, it has not previously been described in lymphoid diseases. Here we report the cases of two patients with B-cell acute lymphocytic leukemia (ALL) having a novel idic(20q−). One was a 34-year-old man with B-cell ALL whose leukemic cells at presentation had a karyotype of 45,XY,dic(9;20)(p11;q11.2); at relapse, a small marker chromosome was found coexisting with the dic(9;20). The other was a 39-year-old woman with Ph-positive B-cell-ALL whose leukemic cells contained both t(9;22)(q34;q11.2) and a small marker chromosome. A series of FISH analyses using the appropriate probes revealed the small marker chromosome in both patients to be an idic(20q−), confirming the dic(9;20)(p11;q11.2) in one case and revealing a BCR/ABL fusion gene in the other. One patient achieved complete remission but relapsed; the other did not achieve complete remission. Both patients died with a short survival time, despite receiving intensive chemotherapy. These two cases show that idic(20q−) can appear not only in myeloid diseases but also in lymphoid diseases. [Copyright &y& Elsevier]

Abstract: Translocations involving 21q22 are commonly observed in both de novo and therapy-related acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). They often result in the disruption of RUNX1 and give rise to fusion genes consisting of RUNX1 and different partner genes, which contribute to leukemogenesis. To date, at least 21 such translocations are known from the literature. Here we report two novel translocations involving the RUNX1 gene: t(1;21)(q12;q22) in a 53-year-old woman with AML-M5b and t(11;21)(q13;q22) in a 65-year-old man with AML-M2. The abnormalities revealed by R-banding karyotypic analysis were confirmed with interphase and metaphase fluorescence in situ hybridization (FISH), chromosome painting, and M-FISH. [Copyright &y& Elsevier]

Here we report a rare chromosomal translocation, t(12;22)(p13;q11), which was detected in a 53-year-old female patient diagnosed as having minimally differentiated acute myeloid leukemia (AML-M0) according to the French-American-British classification criteria. Chromosome painting analysis with probes for chromosomes 12 and 22 confirmed the result of the conventional cytogenetic analysis. Reverse transcriptase polymerase chain reaction revealed the TEL-MN1 fusion transcript. Interestingly, she presented primary multidrug resistance and did not respond to several kinds of chemotherapy regimens. Moreover, she could not achieve remission after two doses of monotherapy with Mylotarg. Flow cytometry analysis detected high levels of expression of P-glycoprotein, multidrug-resistant-related protein, lung-related protein, and glutathione S-transferase π in this case at presentation. As far as we know, this is the first report of t(12;22)(p13;q11) translocation involving TEL and MN1 genes in an AML-M0 patient. Copyright © 2007 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Abstract: A clinical and experimental study of acute lymphoblastic leukemia (ALL) with a dic(7;9)(p11;p11) included 7 patients (5 males and 2 females) with a median age of 32 years. Cytogenetic analysis of bone marrow cells using an R-band technique showed a solitary dic(7;9) in 2 cases and a dic(7;9) accompanied by other abnormalities in 5 cases, of which 4 had also a t(9;22)(q34;q11). A minor breakpoint region (m-bcr) BCR/ABL rearrangement, corresponding to the fusion product gene p190, was detected by interphase FISH in 3 of these cases, Immunophenotyping analysis classified five of the 6 cases as B-lineage ALL. In 6 cases, FISH using whole chromosome painting (WCP) probes for chromosome 7 and 9 confirmed the reciprocal translocation between chromosomes 7 and 9; centromeric probes for chromosomes 7 and 9 confirmed the dicentric nature of the abnormal chromosome. The outcome of these ALL cases with simultaneous dic(7;9) and t(9;22) was worse than that of cases with an isolated dic(7;9). Our study brings the total number of published ALL cases with a dic(7;9) to 18. We consider the dic(7;9) a rare but recurrent abnormality, which may represent a distinct cytogenetic subgroup in B-ALL. [Copyright &y& Elsevier]

We report on seven patients with myeloid diseases characterized by i(20q−) anomaly. Four patients were male and three were female, their median age was 57 years. The diagnosis at presentation was myelodysplastic syndrome in six patients, acute myeloid leukaemia in one patient. Four died but three survived and remain anaemic. The survivals were 6 months for patient 1, 7 months for patient 2, 17 d for patient 4 and 28 d for patient 5. Chromosome specimens were prepared by direct and/or short-term culture of bone marrow cells. Karyotype analysis was performed by R- and G-banding technique, which showed that one of the normal chromosomes 20 was substituted by one or two small metacentric chromosomes in all seven patients. The karyotype was ider(20)(q10)del(20)(q11q13), i.e. i(20q−) in six patients by dual-colour fluorescence in situ hybridization assay using two probes (a subtelomeric probe for 20q and an unique probe for 20q12). As far as we know, this anomaly has not been reported previously. Thus, we consider that i(20q−) is a novel and rare recurrent chromosomal abnormality that is specifically associated with myeloid diseases and may indicate a poor prognosis. [ABSTRACT FROM AUTHOR]

A rare chromosomal translocation, (11;20)(p15;q11), was detected in a 29-year-old male patient diagnosed with acute monocytic leukemia (AMoL) according to the French–American–British classification criteria. Whole chromosome painting analysis with paints for chromosomes 11 and 20 confirmed the result of conventional cytogenetic analysis. Reverse transcriptase polymerase chain reaction revealed the NUP98-TOP1 fusion transcript. To our knowledge, this is the second report of the translocation involving NUP98 and TOP1 genes in AMoL. On reviewing the literature, we suggest that t(11;20)(p15;q11) is associated with myelocytic disorders rather than lymphocytic proliferative diseases. [Copyright &y& Elsevier]

Discusses the case of a 47-year-old woman with a variant form of t(8;21) translocation in acute myeloblastic leukemia (AML) patients. Karyotype; Detection of AML1/ETO chimeric fusion gene; Morphologic features of the patient; Localization of chromosome translocations.

Analyzes the Streptomyces venezuelae pikD gene from the pikromycin biosynthetic cluster. Amino acid homolog of the pikD deduced product PikD with a small family of bacterial regulatory protein; PikD-mediated transcriptional regulation; PikD mutagenesis; Antibiotic production, isolation and analysis; Gene disruption; DNA sequencing and analysis.

Reports findings of a study that provide insight into the structure and function of modular polyketide synthases as well as a new set of tools to generate structural diversity in polyketide natural products. Observation that modular polyketide synthases are giant multifunctional ezymes that catalyze the condensation of small caroxylic acids such as acetate and propionate; Details of the generation of two macrolactone structures; Possible applications.

Presents information on a study which describes the isolation, characterization, and analysis of the biosynthetic gene cluster for methymycin, neomethymycin, narbomycin, and pikromycin in Streptomyces venezuelae. Methodology and materials used to conduct the study; Nucleotide sequence of the pik cluster; Cloning and identification of the pik cluster; Findings of the study.

Abstract: Acute promyelocytic leukemia (APL) is characterized by the presence of a chromosomal rearrangement involving retinoic acid receptor alpha (RARα) gene generating the X-RARα fusion. We describe here a unique RARα gene rearrangement in a patient with M3r subtype of APL. Conventional cytogenetic analysis revealed Y-chromosome loss as the sole karyotypic anomaly. No X-RARα fusion was detected by fluorescence in situ hybridization (FISH) using PML/RARα dual-color dual-fusion translocation probe set, or RARα dual-color break apart rearrangement probe or reverse-transcription polymerase chain reaction (RT-PCR). However, FISH using RARα dual-color break apart rearrangement probe showed a deletion of the entire 3′-end of one allele of RARα gene. To our knowledge, this is the first documented APL with 3′RARα submicroscopic deletion which is not associated with X-RARα fusion. The molecular consequences of this anomaly remain to be elucidated. [Copyright &y& Elsevier]

Highly radio-loud quasars (HRLQs; log  R > 2.5) at z ≳ 4 show apparent enhanced X-ray emission compared to matched HRLQs at lower redshifts, perhaps due to a redshift-dependent fractional contribution to the X-ray luminosity from inverse-Compton scattering of cosmic microwave background photons (IC/CMB). Using new Chandra observations and archival X-ray data, we investigate this phenomenon with an optically flux-limited sample of 41 HRLQs at z  = 4–5.5 all with sensitive X-ray coverage, the largest sample utilized to date by a wide margin. X-ray enhancements are assessed using X-ray-to-optical flux ratios and spectral energy distributions. We confirm the presence of X-ray enhancements at a 4.9–5.3σ significance level, finding that the median factor of enhancement is ≈1.8 at our sample median redshift of z ≈ 4.4. Under a fractional IC/CMB model, the expected enhancement at lower redshifts is modest; e.g. ≈4 per cent at z ≈ 1.5. We also investigate a sample of seven radio-loud quasars (RLQs; log  R > 1) at even higher redshifts of z  = 5.6–6.8, using new and archival X-ray data. These RLQs also show evidence for X-ray enhancements by a median factor of ≈2.7 at a 3.7–4.9σ significance level. The X-ray spectral and other properties of these z  = 5.6–6.8 RLQs, however, pose challenges for a straightforward fractional IC/CMB interpretation of their enhancements. [ABSTRACT FROM AUTHOR]

The UV/optical light curves of Active Galactic Nuclei (AGNs) are commonly described by the Damped Random Walk (DRW) model. However, the physical interpretation of the damping timescale, a key parameter in the DRW model, remains unclear. Particularly, recent observations indicate a weak dependence of the damping timescale upon both wavelength and accretion rate, clearly being inconsistent with the accretion-disk theory. In this study, we investigate the damping timescale in the framework of the Corona Heated Accretion disk Reprocessing (CHAR) model, a physical model that describes AGN variability. We find that while the CHAR model can reproduce the observed power spectral densities of the 20 yr light curves for 190 sources from Stone et al., the observed damping timescale, as well as its weak dependence on wavelength, can also be well recovered through fitting the mock light curves with DRW. We further demonstrate that such weak dependence is artificial due to the effect of inadequate durations of light curves, which leads to best-fitting damping timescales lower than the intrinsic ones. After eliminating this effect, the CHAR model indeed yields a strong dependence of the intrinsic damping timescale on the bolometric luminosity and rest-frame wavelength. Our results highlight the demand for sufficiently long light curves in AGN variability studies and important applications of the CHAR model in such studies. [ABSTRACT FROM AUTHOR]

To investigate the lifetimes of red sequence (RS), blue cloud (BC), and green valley (GV) galaxies, we derive their lifetimes using clustering analyses at 0.5 ≤ z ≤ 2.5 in the COSMOS/UltraVISTA field. Several essentials that may influence the lifetime estimation have been explored, including the dark matter (DM) halo mass function (HMF), the width of the redshift bin, the growth of DM halos within each redshift bin, and the stellar mass. We find that the HMF difference results in scatters of ∼0.2 dex on the lifetime estimation, adopting a redshift bin width of Δz = 0.5 is good enough to estimate the lifetime, and no significant effect on lifetime estimation is found due to the growth of DM halos within each redshift bin. The galaxy subsamples with higher stellar masses generally have shorter lifetimes, but the lifetimes in different subsamples at z > 1.5 tend to be independent of stellar mass. Consistently, the clustering-based lifetime for each galaxy subsample agrees well with that inferred using the spectral energy distribution modeling. Moreover, the lifetimes of the RS and BC galaxies also coincide well with their typical gas-depletion timescales attributed to the consumption of star formation. Interestingly, the distinct lifetime behaviors of the GV galaxies at z ≤ 1.5 and z > 1.5 cannot be fully accounted for by their gas-depletion timescales. Instead, this discrepancy between the lifetimes and gas-depletion timescales of the GV galaxies suggests that there are additional physical processes, such as feedback of active galactic nuclei, which accelerates the quenching of GV galaxies at high redshifts. [ABSTRACT FROM AUTHOR]

We present single-epoch black hole mass (MBH) estimators based on the rest-frame ultraviolet (UV) Mg ii 2798 Å and optical Hβ 4861 Å emission lines. To enlarge the luminosity range of active galactic nuclei (AGNs), we combine the 31 reverberation-mapped AGNs with relatively low luminosities from Bahk et al., 47 moderate-luminosity AGNs from Woo et al., and 425 high-luminosity AGNs from the Sloan Digital Sky Survey. The combined sample has a monochromatic luminosity at 5100 Å in the range erg s−1, over the range 5.5 < log(MBH/M⊙) < 9.5. Based on the fiducial mass from the line dispersion or FWHM of Hβ paired with the continuum luminosity at 5100 Å, we calibrate the best-fit parameters in the black hole mass estimators using the Mg ii line. We find that the differences in the line profiles between Mg ii and Hβ have significant effects on calibrating the UV MBH estimators. By exploring the systematic discrepancy between the UV and optical MBH estimators as a function of AGN properties, we suggest adding a correction term in the equation for the UV mass estimator. We also find a ∼0.1 dex bias in the MBH estimation due to the difference in the spectral slope in the range 2800–5200 Å. Depending on whether the selection of MBH estimator is based on either line dispersion or FWHM and either continuum or line luminosity, the derived UV mass estimators show ≳0.1 dex intrinsic scatter with respect to the fiducial Hβ-based MBH. [ABSTRACT FROM AUTHOR]

We investigate the intrinsic spectral energy distribution (SED) of active galactic nuclei (AGNs) at infrared (IR) bands with 42 z < 0.5 optically luminous Palomar Green survey quasars through SED decomposition. We decompose the SEDs of the 42 quasars by combining an AGN IR template library that covers a wide range of the AGN parameter space with three commonly used galaxy template libraries. We determine the median AGN SED from the best-fitting results. The far-IR (FIR) contribution of our median AGN SED is significantly smaller than that of Symeonidis et al., but roughly consistent with that of Lyu & Rieke. The AGN IR SED becomes cooler with increasing bolometric luminosity, which might be due to the fact that more luminous AGNs might have stronger radiative feedback to change torus structures and/or their tori might have higher metallicities. Our conclusions do not depend on the choice of galaxy template libraries. However, as the predicted polycyclic aromatic hydrocarbon (PAH) emission line flux is galaxy template dependent, cautions should be taken on deriving galaxy FIR contribution from PAH fluxes. [ABSTRACT FROM AUTHOR]

The Australia Telescope Large Area Survey (ATLAS) and the VLA survey in the XMM-LSS/VIDEO deep field provide deep (≈15 |$\mu$| Jy beam−1) and high-resolution (≈4.5–8 arcsec) radio coverage of the three XMM-SERVS fields (W-CDF-S, ELAIS-S1, and XMM-LSS). These data cover a total sky area of 11.3 deg2 and contain ≈11 000 radio components. Furthermore, about 3 deg2 of the XMM-LSS field also has deeper MIGHTEE data that achieve a median RMS of 5.6 |$\mu$| Jy beam−1 and detect more than 20 000 radio sources. We analyse all these radio data and find source counterparts at other wavebands utilizing deep optical and infrared (IR) surveys. The nature of these radio sources is studied using radio-band properties (spectral slope and morphology) and the IR–radio correlation. Radio AGNs are selected and compared with those selected using other methods (e.g. X-ray). We found 1656 new AGNs that were not selected using X-ray and/or MIR methods. We constrain the FIR-to-UV SEDs of radio AGNs using cigale and investigate the dependence of radio AGN fraction upon galaxy stellar mass and star formation rate. [ABSTRACT FROM AUTHOR]

We report results for a complete sample of 10 luminous radio-quiet quasars with large C iv equivalent widths (EW ≥ 150 Ă...). For 8/10 we performed Chandra snapshot observations. We find that, in addition to the enhanced C iv line EW, their He ii and Mg ii lines are enhanced, but the C iii ] line is not. Their X-ray emission is substantially stronger than expected from their ultraviolet luminosity. Additionally, these large C iv EW quasars show small C iv blueshifts and possibly low Eddington ratios, suggesting that they are “extreme low Eigenvector 1 (EV1)” quasars. The mean excess He ii EW is well matched by radiation pressure compression (RPC) photoionization models, with the harder α ox ionizing spectrum. However, these results do not reproduce well the enhancement pattern of the C iv, Mg ii, and C iii ] EWs, or the observed high C iv /Mg ii ratio. RPC calculations indicate that the C iv /Mg ii line ratio is an effective metallicity indicator, and models with subsolar-metallicity gas and a hard ionizing continuum reproduce well the enhancement pattern of all four ultraviolet lines. We find that the C iv /Mg ii line ratio in quasars is generally correlated with the excess X-ray emission. Extremely high EV1 quasars are characterized by high metallicity and suppressed X-ray emission. The underlying mechanism relating gas metallicity and X-ray emission is not clear but may be related to radiation-pressure-driven disk winds, which are enhanced at high metallicity, and consequent mass loading reducing coronal X-ray emission. [ABSTRACT FROM AUTHOR]

The clinical and hematological characteristics and the prognostic significance of del(20q) were investigated in a consecutive series of 213 myeloid malignancies. In the analyses, the cases were divided into three subgroups according to diagnosis or four subgroups according to cytogenetic data. Patients in the myeloproliferative neoplasms subgroup had high WBCs and platelet counts at initial diagnosis. The del(20q) occurred predominantly in older men. Sole del(20q) was observed most often in myelodysplastic syndromes, while del(20q) as a part of complex karyotypes was observed predominantly in acute myeloid leukemia. The most frequent additional abnormalities accompanying del(20q) were −5/del(5q), −7/del(7q) and +8; t(20;21)(q11;q11) and double del(20q) were two rare but recurrent abnormalities secondary to del(20q). In all types of diseases, patients with a sole del(20q) had a favorable prognosis. The presence of any additional abnormality with del(20q) had an unfavorable outcome. Patients with i(20q) had an unfavorable prognosis. Patients with the minor del(20q) clone had a better median survival than those with the major del(20q) clone. [Copyright &y& Elsevier]

The shape of the ambient circumnuclear medium (ACM) density profile can probe the history of accretion onto the central supermassive black holes in galaxies and the circumnuclear environment. However, due to the limitations of instrument resolution, the density profiles of the ACM for most galaxies remain largely unknown. In this work, we propose a novel method to measure the ACM density profile of active galactic nuclei (AGNs) by the equilibrium between the radiation pressure on the warm absorbers (WAs, a type of AGN outflow) and the drag pressure from the ACM. We study the correlation between the outflow velocity and ionization parameter of WAs in each of the five Seyfert 1 galaxies (NGC 3227, NGC 3783, NGC 4051, NGC 4593, and NGC 5548), inferring that the density profile of the ACM is between n ∝ r âˆ'1.7 and n ∝ r âˆ'2.15 (n is number density and r is distance) from 0.01 pc to parsec scales in these five AGNs. Our results indicate that the ACM density profile in Seyfert 1 galaxies is steeper than the prediction by the spherically symmetric Bondi accretion model and the simulated results of the hot accretion flow, but more in line with the prediction by the standard thin-disk model. [ABSTRACT FROM AUTHOR]

Human leukemia cell lines are powerful tools in the study of leukemogenesis, particularly for rare but recurrent subtypes such as acute myeloid leukemia (AML) with the t(16;21)(p11.2;q22) and FUS-ERG fusion. Four AML cell lines carrying a t(16;21)(p11.2;q22) have been described previously. We report a novel AML cell line, designated JIH-4, for which karyotypic analysis demonstrated a single abnormality, t(16;21)(p11.2;q22). The FUS-ERG fusion transcript was identified by reverse transcriptase polymerase chain reaction (RT-PCR). Neither Epstein-Barr virus nor mycoplasma was detected in JIH-4 cells. The morphology and immunoprofile of JIH-4 cells display typical features of myelogenous lineage, and short tandem-repeat PCR comparison with the donor patient’s bone marrow cells confirm the cell line’s authenticity. Tumor masses were found in 50% of inoculated mice 83 days after subcutaneous injection with JIH-4 cells. Our results confirm that JIH-4 cells are derived from the donor patient’s leukemia cells and support using the JIH-4 cell line as a valuable tool in the study of leukemogenesis. [ABSTRACT FROM AUTHOR]

Abstract: Besides cytopenia, dysplasia is crucial characteristic of MDS-RA. To summarize the morphological features that contribute to the diagnosis of MDS-RA, 48 RA patients with abnormal karyotype were analyzed for the features of morphological and cytogenetical abnormalities and the relationships between them. 46 MDS-RA patients with normal karyotype and 207 patients with non-MDS anemia were enrolled into control groups. More conspicuous and diverse dysplasia can be found in abnormal karyotype MDS-RA than those in control groups (P <0.05). Apparent dysplasia in granulocyte and megakaryocytoid lineages may provide valuable evidence for the diagnosis. Dysplasia occurred more frequently in patients with severe chromosome abnormalities. [Copyright &y& Elsevier]

Most bulge‐dominated galaxies host black holes (BHs) with masses that tightly correlate with the masses of their bulges. This may indicate that the BHs may regulate galaxy growth, or vice versa, or that they may grow in lock‐step. The quest to understand how, when, and where those BHs formed motivates much of extragalactic astronomy. Here we focus on a population of galaxies with active BHs in their nuclei (active galactic nuclei [AGN]), that are fully or partially hidden by dust and gas: The emission from the broad line region is either completely or partially obscured with a visual extinction of one or above. This limit, though not yet precise, appears to be the point at which the populations of AGN may evolve differently. We highlight the importance of finding and studying those dusty AGN at redshifts between one and three, the epoch when the universe may have gone through its most dramatic changes. We emphasize the need for future large multiplexed spectroscopic instruments that can perform dedicated surveys in the optical and near‐infrared to pin down the demographics of such objects, and study their reddening properties, star‐formation histories, and excitation conditions. These key studies will shed light on the role of BHs in galaxy evolution during the epoch of peak growth activity. [ABSTRACT FROM AUTHOR]

The relationship between quasars and their host galaxies provides clues on how supermassive black holes (SMBHs) and massive galaxies are jointly assembled. To elucidate this connection, we measure the structural and photometric properties of the host galaxies of ∼5000 Sloan Digital Sky Survey quasars at 0.2 < z < 1 using five-band (grizy) optical imaging from the Hyper Suprime-Cam Subaru Strategic Program. An automated analysis tool is used to forward-model the blended emission of the quasar as characterized by the point-spread function and the underlying host galaxy as a two-dimensional Sérsic profile. We find that quasars are preferentially hosted by massive star-forming galaxies with disklike light profiles. Furthermore, the size distribution of quasar hosts is broad at a given stellar mass and the average values exhibit a size–stellar mass relation as seen with inactive galaxies. In contrast, the sizes of quasar hosts are more compact than those of inactive star-forming galaxies on average, but not as compact as those of quiescent galaxies of similar stellar masses. This is true irrespective of quasar properties, including the bolometric luminosity, Eddington ratio, and black hole mass. These results are consistent with a scenario in which galaxies are concurrently fueling an SMBH and building their stellar bulge from a centrally concentrated gas reservoir. Alternatively, quasar hosts may be experiencing a compaction process in which stars from the disk and inflowing gas are responsible for growing the bulge. In addition, we confirm that the host galaxies of type 1 quasars have a bias of being closer to face-on systems, suggesting that galactic-scale dust can contribute to obscuring the broad-line region. [ABSTRACT FROM AUTHOR]

Context. Spatially resolved observations of the ionized and molecular gas are critical for understanding the physical processes that govern the interstellar medium (ISM) in galaxies. The observation of starburst systems is also important as they present extreme gas conditions that may help to test different ISM models. However, matched resolution imaging at ∼kpc scales for both ISM gas phases are usually scarce, and the ISM properties of starbursts still remain poorly understood. Aims. We aim to study the morpho-kinematic properties of the ionized and molecular gas in three dusty starburst galaxies at z = 0.12−0.17 to explore the relation between molecular ISM gas phase dynamics and the star-formation activity. Methods. We employ two-dimensional dynamical modelling to analyse Atacama Large Millimeter/submillimiter Array CO(1–0) and seeing-limited Spectrograph for INtegral Field Observations in the Near Infrared Paschen-α (Paα) observations, tracing the molecular and ionized gas morpho-kinematics at ∼kpc-scales. We use a dynamical mass model, which accounts for beam-smearing effects, to constrain the CO-to-H2 conversion factor and estimate the molecular gas mass content. Results. One starburst galaxy shows irregular morphology, which may indicate a major merger, while the other two systems show disc-like morpho-kinematics. The two disc-like starbursts show molecular gas velocity dispersion values comparable with those seen in local luminous and ultra luminous infrared galaxies but in an ISM with molecular gas fraction and surface density values in the range of the estimates reported for local star-forming galaxies. We find that these molecular gas velocity dispersion values can be explained by assuming vertical pressure equilibrium. We also find that the star-formation activity, traced by the Paα emission line, is well correlated with the molecular gas content, suggesting an enhanced star-formation efficiency and depletion times of the order of ∼0.1−1 Gyr. We find that the star-formation rate surface density (ΣSFR) correlates with the ISM pressure set by self-gravity (Pgrav) following a power law with an exponent close to 0.8. Conclusions. In dusty disc-like starburst galaxies, our data support the scenario in which the molecular gas velocity dispersion values are driven by the ISM pressure set by self-gravity and are responsible for maintaining the vertical pressure balance. The correlation between ΣSFR and Pgrav suggests that, in these dusty starbursts galaxies, the star-formation activity arises as a consequence of the ISM pressure balance. [ABSTRACT FROM AUTHOR]

We report the discovery of large-amplitude mid-infrared variabilities (MIR; ∼0.3 mag) in the Wide-field Infrared Survey Explorer W 1 and W 2 bands of the low-luminosity narrow-line Seyfert 1 galaxy WPVS 007, which exhibits prominent and varying broad-absorption lines (BALs) with blueshifted velocity up to |${\sim } 14\, 000$| |$\rm km\ s^{-1}$|⁠. The observed significant MIR variability, the UV to optical colour variabilities in the Swift  bands that deviate from the predictions of pure dust attenuation models, and the fact that Swift  light curves can be well fitted by the stochastic AGN variability model suggest that its observed flux variabilities in the UV–optical–MIR bands should be intrinsic, rather than owing to variable dust extinction. Furthermore, the variations of BAL features (i.e. trough strength and maximum velocity) and continuum luminosity are concordant. Therefore, we propose that the BAL variability observed in WPVS 007 is likely induced by the intrinsic ionizing continuum variation, alternative to the rotating-torus model proposed in a previous work. The BAL gas in |$\rm WPVS\ 007$|  might be in the low-ionization state as traced by its weak N  v  BAL feature; as the ionizing continuum strengthens, the C  iv  and Si  iv  column densities also increase, resulting in stronger BALs and the emergence of high-velocity components of the outflow. The outflow launch radius might be as small as ∼8 × 10−4 pc under the assumption of being radiatively driven, but a large-scale origin (e.g. torus) cannot be fully excluded because of the unknown effects from additional factors, e.g. the magnetic field. [ABSTRACT FROM AUTHOR]

The Chinese Space Station Optical Survey (CSS-OS) is a major science project of the Space Application System of the China Manned Space Program. This survey is planned to perform both photometric imaging and slitless spectroscopic observations, and it will focus on different cosmological and astronomical goals. Most of these goals are tightly dependent on the accuracy of photometric redshift (photo- z) measurement, especially for the weak gravitational lensing survey as a main science driver. In this work, we assess if the current filter definition can provide accurate photo- z measurement to meet the science requirement. We use the COSMOS galaxy catalogue to create a mock catalogue for the CSS-OS. We compare different photo- z codes and fitting methods that use the spectral energy distribution template-fitting technique, and choose to use a modified lephare code in photo- z fitting process. Then we investigate the CSS-OS photo- z accuracy in certain ranges of filter parameters, such as band position, width, and slope. We find that the current CSS-OS filter definition can achieve reasonably good photo-z results with σ z  ∼ 0.02 and outlier fraction ∼3 per cent. [ABSTRACT FROM AUTHOR]

This study reports 10 patients with hematological malignances with t(20;21)(q11;q11) resulting from del(20q) (for example, der(20)del(20)(q11q13)t(20;21)(q11;q11) and der(21)t(20;21)(q11;q11)) and described their clinical features and the possible prognostic significance of this abnormality. The t(20;21)(q11;q11) was a rare but recurrent abnormality secondary to del(20q) besides i(20q-). The frequency of der(20)del(20)(q11q13)t(20;21)(q11;q11) among our patients with del(20q) was 2.4%. It was considered that the 20q deletion preceded translocation with chromosome 21. This abnormality is often cryptic, occurs predominantly in older men and is observed most often in myelodysplastic syndromes. Patients with this abnormality have an unfavorable prognosis, similar to patients with i(20q-). The molecular consequences of der(20)del(20)(q11q13)t(20;21)(q11;q11) may be different from patients with i(20q-). To the best of our knowledge this is the largest dataset published to date. [ABSTRACT FROM AUTHOR]

Acute promyelocytic leukemia (APL) is characterized by the generation of the PML-RARα fusion transcript as a result of a reciprocal chromosomal rearrangement, t(15;17)(q22;q12), with breakpoints within the PML gene and the RARα gene. In a small proportion of APL cases, RARα is fused with a number of alternative partner genes. The signal transducer and activator of transcription 5 beta (STAT5b) is one of the variant partners. Here, we describe one rare case with all-trans retinoic acid (ATRA) −unresponsive APL characterized by the STAT5b-RARα fusion transcript. Morphology and immunophenotypic analyses indicated the typical features of APL; however, cytogenetic analysis exhibited a normal karyotype, and importantly, results of interphase fluorescence in situ hybridization (FISH) or reverse transcriptase-polymerase chain reaction (RT-PCR) analysis indicated that PML-RARα expression was negative. FISH analysis with the RARα dual-color break-apart rearrangement probe indicated a submicroscopic deletion of the 3'' end of one RARA gene. Indeed, the STAT5b-RARα fusion transcript was found in this case by array-based comparative genomic hybridization and nested RT-PCR. To the best of our knowledge, we report here only the sixth APL patient in the world with the STAT5b-RARα fusion transcript. Additional clinical studies concerning the prognosis, response to therapy, and pathogenesis of APL patients with STAT5b-RARα fusion are necessary. [Copyright &y& Elsevier]

The article examines the occurrence of somatic BRAF V600E mutation in patient suffering from hematologic malignancies. The study denies the presence of evidence of BRAF mutations that involves exon 15 in patients. Noted is the detection of BRAF V600E mutation in bone marrow mononuclear cells in patients with hairy cell leukemia.