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Amino acid oxidases (AOs) are flavin adenine dinucleotide (FAD)-dependent dimeric enzymes that stereo specifically catalyse the deamination of an Ą-amino acid leading to an Ą-keto acid. Putative Leptospira interrogans recombinant L-amino acid oxidase (Li-rLAO; lacking 20 residues corresponding to the N-terminal signal sequence) was cloned, expressed, purified, and its three-dimensional structure was determined by X-ray crystallography at a resolution of 1.8 A. The active site could be easily identified by the presence of electron density corresponding to a non-covalently bound FAD in both protomers of the dimeric enzyme. Structural analysis of Li-rLAO revealed that its polypeptide fold is similar to those of the previously determined homologous structures as available in the Protein Data Bank. However, a substrate-binding residue found at the active site of other previously determined homologous structures was not conserved in Li-rLAO, suggesting that its specificity may differ from those of earlier reported structures. Not surprisingly, Li-rLAO showed no activity for most amino acids and amines; it exhibited a low activity only with L-arginine as the substrate. The catalytic properties of Li-rLAO could be rationalized in terms of its three-dimensional structure. [ABSTRACT FROM AUTHOR]

The crystal structure of an uncharacterized hypothetical protein, TTHA1873 from Thermus thermophilus, has been determined by X‐ray crystallography to a resolution of 1.78 Å using the single‐wavelength anomalous dispersion method. The protein crystallized as a dimer in two space groups: P43212 and P6122. Structural analysis of the hypothetical protein revealed that the overall fold of TTHA1873 has a β‐sandwich jelly‐roll topology with nine β‐strands. TTHA1873 is a dimeric metal‐binding protein that binds to two Ca2+ ions per chain, with one on the surface and the other stabilizing the dimeric interface of the two chains. A structural homology search indicates that the protein has moderate structural similarity to one domain of cell‐surface proteins or agglutinin receptor proteins. Red blood cells showed visible agglutination at high concentrations of the hypothetical protein. [ABSTRACT FROM AUTHOR]

The next generation sequencing (NGS) technology generates a large amount of genomic data. Bioinformatics has made it a priority to deal with genomic data in all of its forms. The main focus of this article is on the use of data mining in bioinformatics. The processing flow of bioinformatics data is depicted using a summary of the definition and relevant research contents of bioinformatics. Then emphatically introduces data mining from the perspective of data preprocessing, dimension reduction and statistical machine learning in bioinformatics. The use of data mining in the field of bioinformatics is discussed. It also discusses some of the current obstacles and prospects in bioinformatics data mining. [ABSTRACT FROM AUTHOR]