1. Direct and Simultaneous Identification of Multiple Mitochondrial Reactive Oxygen Species in Living Cells Using a SERS Borrowing Strategy.
- Author
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Lin, Shichao, Ze, Huajie, Zhang, Xia‐Guang, Zhang, Yue‐Jiao, Song, Juan, Zhang, Huimin, Zhong, Han‐Liang, Yang, Zhi‐Lan, Yang, Chaoyong, Li, Jian‐Feng, and Zhu, Zhi
- Abstract
Identification of different mitochondrial reactive oxygen species (ROS) simultaneously in living cells is vital for understanding the critical roles of different ROS in biological processes. To date, it remains a great challenge to develop ROS probes for direct and simultaneous identification of multiple ROS with high specificity. Herein, we report a SERS‐borrowing‐strategy‐based nanoprobe (Au@Pt core–shell nanoparticles) for simultaneous and direct identification of different ROS by their distinct Raman fingerprints. Isotope substitution experiments and DFT calculations confirmed the ability of Au@Pt nanoprobe to capture and identify different mitochondrial ROS (i.e. ⋅OOH, H2O2, and ⋅OH). When functionalized with triphenylphosphine (TPP), the Au@Pt‐TPP nanoprobe located to mitochondria and detected multiple ROS simultaneously in living cells under oxidative stimulation. Our method offers a new tool for the study of the functions of various ROS in biological processes. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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