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microRNAs (miRNAs) are critically involved in liver regeneration (LR). miR-125a-5p (miR-125a) is a tumor-suppressing miRNA, but its role in LR has not been studied. Our previous studies have proved that miR-125a was related to LR at the initiation phase, while the mechanism hepatocyte proliferation triggered by miR-125a in LR has been rarely evaluated. Herein, we mainly studied the molecular mechanism of miR-125a in triggering hepatocyte proliferation and the proliferation stage of LR. Firstly, a striking reduction of miR-125a was found at 24 h as well as 30 h following partial hepatectomy (PH) in rat liver tissue by miRNAs expression profiles as well as qRT-PCR analysis. Furthermore, in vitro, upregulation of miR-125a decreased proliferation as well as G1/S conversion, which promoted hepatocytes apoptosis. STAT3 was the target of miR-125a. In vivo, upregulation of miR-125a by tail vein injection of agomir inhibited LR index. Upregulation of miR-125a inhibited LR index and hepatocytes proliferation by STAT3/p-STAT3/JUN/BCL2 axis. In summary, these current discoveries indicated that miR-125a inhibited hepatocytes proliferation as well as LR by targeting STAT3 and via acting on the STAT3/p-STAT3/JUN/BCL2 axis. [ABSTRACT FROM AUTHOR]

The zeotropic mixture is a feasible way to improve the thermodynamics performance of organic Rankine cycle (ORC). A small-scale ORC experimental apparatus with a scroll expander was established. Then, the system performance using different zeotropic mixtures (including 0.75R245fa/0.25R141b, 0.5R245fa/0.5R141b and 0.25R245fa/0.75R141b) was tested and compared with the pure fluid of R245fa and R141b to determine whether the mixture fluid can improve the system performance. The experimental results show that the thermal efficiency and exergy efficiency of ORC system gradually increase with the increase of cooling water flow rate and the decrease of expander rotational speed. Under different working conditions, mixture fluids can produce more shaft power and have lower condenser exergy destruction than pure fluids. The zeotropic mixture is not always better than the pure fluid, while it can improve the thermal efficiency and exergy efficiency of the ORC system with an appropriate mass fraction. In the experiment, the best mass fraction of R245fa/R141b is 0.25/0.75, and its thermodynamic performance is significantly higher than that of R141b and R245fa. The maximum thermal efficiency and exergy efficiency of 0.25R245fa/0.75R141b are 5.58% and 23.2% respectively. • ORC system performance using R245fa/R141b was tested and compared with its pure component. • Effects of coolant flow rate and expander rotational speed on system performance is studied. • Mixture fluid with a suitable mass fraction can improve ORC system performance. • 0.25R245fa/0.75R141b is the best fluid with a maximum exergy efficiency of 23.2%. [ABSTRACT FROM AUTHOR]

Development of latent fingerprints (LFPs) at the scene played the important role in forensic investigations by reconstructing crime trajectory and individualizing suspect. In this work, the optical sensor named TPE‐LL with aggregation‐induced emission property was constructed and used in LFPs visualization. The sensor exhibited a satisfactory linear relationship to tryptophan (Trp) in the range of 1–20 nM with the low detection limit of 0.15 nM. The samples were collected from 60 volunteers, with a total of 7200 impressed samples and 160 natural samples. Significantly, the high‐resolution fingerprints images with high fidelity and contrast containing pore features were obtained from impressed samples left on porous object for 15 days. Meanwhile, the relative complete STR typing profiles of deposited DNA were obtained and the identification rate of DNA after the develop process was 76.5 %, suggesting that the method was low destructive to DNA. The results indicated that the sensor provided promising perspectives on solving the current technical problems in the field of forensic science and technology [ABSTRACT FROM AUTHOR]

Sarcoidosis is a systemic granulomatous disease of the lungs that commonly involves intrathoracic lymph nodes. Here, we report the case of a 68-year-old woman who complained of shortness of breath and had suffered from the enlargement of intrathoracic lymph nodes for 12 years, swelling of the right middle finger for 7 years, and nasal obstruction for 2 years. The damage to the phalange was aggravated continuously and a malignant lesion could not be excluded, thus leading to amputation of the right middle finger. Pathological data indicated chronic inflammatory granulomatous disease and anti-acid staining was negative. Examination of the paranasal sinuses indicated destruction of the sinonasal bone and the swollen mucosa. Combined with the elevated ratio of CD4+/CD8+ T cells in bronchoalveolar lavage fluid and other results, the patient was finally diagnosed with sarcoidosis and received corticosteroid therapy. The shortness of breath and abnormality of the nose were significantly improved after treatment. Our case demonstrated the process of differential diagnosis for systemic granulomatous diseases, indicating the necessity of corticosteroid therapy for systematic sarcoidosis. [ABSTRACT FROM AUTHOR]

Soybean is a major crop that provides oil and protein worldwide. Soybean products have been consumed for centuries in many different kinds of food. The regulatory network that controls the accumulation of fatty acids (FAs) and storage proteins (SSPs) in seeds is poorly understood. To gain new insights into the molecular mechanisms that contribute to high‐quality seeds, seed FA and SSP contents were analyzed for the effects of chromosome segment substitution in a wild soybean line (CSSL). High‐throughput transcriptomics and tandem mass tag (TMT)‐based quantitative proteomics were performed on the dry seeds of the CSSL and parent lines. In total, 665 differentially expressed genes (DEGs) and 83 differentially accumulated proteins (DAPs) were identified. Of these 27 DEGs and 23 DAPs were found to regulate the seed storage profile. These genes encode proteins involved in photosynthesis, protein processing, protein sorting, and storage protein accumulation. Data are presented showing that FA synthesis was decreased by the regulation of SSP‐accumulation‐related genes and proteins. Taken together, the results provide new insights into the regulation network involved in the accumulation of seed storage compounds involving both source and sink processes that may be used as markers in future soybean breeding programs. [ABSTRACT FROM AUTHOR]

The secretion, accumulation, and composition of extracellular polymeric substances (EPS) are pivotal factors influencing microalgal growth as well as wastewater recycling. Until now, the accumulation and inhibition mechanism of EPS of Chlorella vulgaris during cycling cultivation is not fully understood. The purpose of this study was to explore how different light qualities regulate the secretion, chemical composition, and structure of microalgal EPS, and subsequently influence the recycling of culture wastewater. After four cycles of cultivation, C. vulgaris under green light produced the highest EPS production and lowest biomass production, which were 82% higher and 17% lower, respectively, compared to white light, which yielded the least EPS production and the highest biomass production. EPS under different light qualities all exhibited a fibrillar structure with a sheet-like surface, but differed in composition. Compared with the other groups, EPS under green light showed a significant increase in polysaccharides, proteins, and humic acid-like compounds, as well as an increased proportion of arabinose and rhamnose, according to monosaccharide composition analysis. Transcriptome analysis indicated that the up-regulation of metabolic pathways linked to glycolysis/gluconeogenesis, TCA cycle, lipid synthesis, and ABC transporters promoted EPS accumulation. Additionally, EPS could target light-harvesting complex (LHC) and electron transport chain, down-regulating the photosynthetic pathway, which ultimately inhibited microalgal growth under green light. This study provides a theoretical foundation for the light regulation and circulation culture of microalgae, as well as for microalgal wastewater treatment. [Display omitted] • EPS can accumulate in recycled wastewater and inhibited C. vulgaris growth. • Green light produced the highest EPS production, 82% higher than white light. • EPS was composed of polysaccharides, protein-like and humic acid-like compounds. • EPS accumulation and inhibition mechanism during cycling cultivation was revealed. [ABSTRACT FROM AUTHOR]

The organic Rankine cycle coupled vapor compression refrigeration system is an attractive refrigeration technology that converts low-grade thermal energy into cooling capacity to improve waste heat utilization efficiency. Due to the inevitable change in ambient temperature and cooling demand, the dynamic characteristics of the combined system are worth studying. In this paper, a small experimental apparatus with a common condenser is designed and developed. The dynamic characteristic experiments under different disturbances are carried out to understand the response characteristics of operating parameters and the variation of system performance. The results show that the maximum response time of the system under different disturbances ranges from 493 s to 694 s. The impact of throttle opening on the response characteristics of refrigeration subsystem parameters is significantly higher than that of the organic Rankine cycle. The mass flow rate of organic Rankine cycle has the fastest response to pump frequency, while the compressor inlet temperature changes rapidly with the cooling water temperature. Under a step cooling water temperature, it is important to control the compressor inlet temperature to avoid liquid droplets in its inlet. In addition, the mass flow rate of organic Rankine cycle should be controlled reasonably to achieve the maximum coefficient of system performance. The cooling capacity of the system is mainly affected by the cooling water temperature and the throttle valve opening. During the experiment, the maximum cooling capacity and performance coefficient of the system are 3.75 kW and 0.275, respectively. This work can provide guidance for the design of the control strategy of the combined system. • An organic Rankine cycle coupled vapor compression refrigeration system was built. • Dynamic characteristics of the combined system under various disturbances were tested. • Response time and change rate of main parameters were provided. • System has a large thermal inertia with a maximum response time of 694 s. [ABSTRACT FROM AUTHOR]

[Display omitted] • A novel visualization strategy based on Eu@Tb-LL for Gly detection was designed. • Eu@Tb-LL improved visualization contrast of commercial manifestation reagent. • Eu@Tb-LL is more effective than indandione in revealing aged fingerprints. • First example of evaluating fingerprints and DNA data based on cluster analysis. Fingermarks identification have always been the most effective and reliable way for individuals determination among the forensic science. The fingermarks at the scene of the case as the valuable physical evidence to analyze the trajectory of criminal behavior and portray the characteristics of the suspect play an irreplaceable role in the process of investigation. To reach its pivotal biometric individualization, the luminescent metal–organic framework (Eu@Tb-LL) was prepared via a simple optimized solvothermal method for visualizing the latent fingermarks with minimally destructive to DNA in this work. Eu@Tb-LL exhibited ideal optical stability and fluorescence intensity at the wavelength of 530 and 620 nm during revealing latent fingermarks. Meanwhile, Eu@Tb-LL is more effective than indandione (commonly used in the site investigation) in revealing aged fingermarks. More significantly, the Eu@Tb-LL superimposed on commercial manifestation reagents (Hfb) enhanced the effect of Hfb for revealing blood latent fingermarks on the non-porous objects. In addition, the STR testing results performed after the visualization process proved the Eu@Tb-LL was minimally destructive to DNA with a confidence interval of 95 %, exhibiting promising prospects for practical criminal investigation applications. [ABSTRACT FROM AUTHOR]

There is growing interest in expanding the production of soybean oils (mainly triacylglycerol, or TAG) to meet rising feed demand and address global energy concerns. We report that a plastid‐localized glycerol‐3‐phosphate dehydrogenase (GPDH), encoded by GmGPDHp1 gene, catalyzes the formation of glycerol‐3‐phosphate (G3P), an obligate substrate required for TAG biosynthesis. Overexpression of GmGPDHp1 increases soybean seed oil content with high levels of unsaturated fatty acids (FAs), especially oleic acid (C18:1), without detectably affecting growth or seed protein content or seed weight. Based on the lipidomic analyses, we found that the increase in G3P content led to an elevated diacylglycerol (DAG) pool, in which the Kennedy pathway‐derived DAG was mostly increased, followed by PC‐derived DAG, thereby promoting the synthesis of TAG containing relatively high proportion of C18:1. The increased G3P levels induced several transcriptional alterations of genes involved in the glycerolipid pathways. In particular, genes encoding the enzymes responsible for de novo glycerolipid synthesis were largely upregulated in the transgenic lines, in‐line with the identified biochemical phenotype. These results reveal a key role for GmGPDHp1‐mediated G3P metabolism in enhancing TAG synthesis and demonstrate a strategy to modify the FA compositions of soybean oils for improved nutrition and biofuel. [ABSTRACT FROM AUTHOR]

In this work, α-Fe2O3 double-layer hollow spheres with carbon coating (DLHS@C) are synthesized by a general "penetration-heterogeneous nucleation" approach using carbonaceous sphere templates combined with subsequent annealing. This hierarchy hollow sphere structure with carbon coating rationally combines three advantage aspects: shorten transport length for lithium ions and charges, sufficient void space to buffer the volume expansion, and enhanced electric conductivity. When tested for lithium storage, α-Fe2O3 DLHS@C composite electrode exhibits a high initial discharge capacity of 1735 mAh g-1 and the reversible capacity of 823 mAh g-1 after 100 cycles at the current density of 100 mA g-1 in the voltage range of 0.01–2.5 V (vs. Li+/Li) and retains a capacity of 470 mAh g-1 even at a large current density of 500 mA g-1 after 500 cycles. The α-Fe2O3 DLHS@C electrode demonstrates excellent electrochemical properties with high capacity, good cycling stability, and excellent rate capability as potential anode material for lithium ion batteries. [ABSTRACT FROM AUTHOR]

Despite of the high demand for liquefied natural gas (LNG) in demanding countries, an ideal means for establishing the LNG supply system has not yet been found in many regions. In this paper, a three-stage stochastic programming method has been proposed for LNG supply system infrastructure development and inventory routing in demanding countries. The minimum daily cost is set as the objective function; and the cost consists of the delivery cost, liquefaction cost, purchase cost, and construction cost. Under the constraints of delivery mode, volume, vehicle, time, and infrastructure construction, a multi-scenario MILP model was established and solved by a hybrid computational method (ACO-MILP), and the optimal infrastructure development and inventory routing were presented as the result. Finally, the method was successfully applied to the LNG supply system along the Yangtze River in China. Furthermore, compared with the other methods, the superiority of the method was verified. [ABSTRACT FROM AUTHOR]