1. The combination of Butyricicoccus pullicaecorum and 3-hydroxyanthranilic acid prevents postmenopausal osteoporosis by modulating gut microbiota and Th17/Treg.
- Author
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Zhu, Fuping, Liu, Hui, Cao, Yinsheng, Dai, Bing, Wu, Hang, and Li, Wuping
- Subjects
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OSTEOPOROSIS prevention , *PREVENTIVE medicine , *COMBINATION drug therapy , *FLOW cytometry , *STATISTICAL correlation , *T cells , *AUTOPHAGY , *HOMEOSTASIS , *GUT microbiome , *POSTMENOPAUSE , *HYDROXY acids , *ANIMAL experimentation , *RESEARCH , *PROBIOTICS , *BUTYRIC acid , *GRAM-positive bacteria , *SEQUENCE analysis , *PSEUDOMONAS - Abstract
Background: Postmenopausal osteoporosis (PMO) is a chronic condition characterized by decreased bone strength. This study aims to investigate the effects and mechanisms of the combination of Butyricicoccus pullicaecorum (Bp) and 3-hydroxyanthranilic acid (3-HAA) on PMO. Methods: The effects of Bp and 3-HAA on PMO were evaluated in ovariectomized (OVX) rats by assessing stereological parameters, femur microstructure, and autophagy levels. The T helper (Th) 17/Regulatory T (Treg) cells of rats were detected using flow cytometric analysis. Furthermore, the impact of Bp and 3-HAA on the gut microbiota of rats was assessed using 16S rRNA gene sequencing. The correlation between the gut microbiota of rats and Th17/Treg immune factors, as well as femoral stereo parameters, was separately assessed using Spearman rank correlation analysis. Results: Bp and 3-HAA treatments protected OVX rats by promoting osteogenesis and inhibiting autophagy. Compared to the Sham group, OVX rats showed an increase in Th17 cells and a decrease in Treg cells. Bp and 3-HAA reversed these changes. Enterorhabdus and Pseudomonas were significantly enriched in OVX rats. Bp and 3-HAA regulated the gut microbiota of OVX rats, enriching pathways related to nutrient metabolism and immune function. There was a correlation between the gut microbiota and the Th17/Treg, as well as femoral stereo parameters. The concurrent administration of Bp and 3-HAA medication facilitated the enrichment of gut microbiota associated with the improvement of PMO. Conclusion: The combination therapy of Bp and 3-HAA can prevent PMO by modulating the gut microbiota and restoring Th17/Treg immune homeostasis. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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