1. Antimicrobial activity of the crude extract, fractions and isolation of zerumbone from the rhizomes of Zingiber roseum.
- Author
-
Al-Amin, Mohammad, Siddiqui, Mohammad Arafat, Ruma, Shamima Afrin, Eltayeb, Nagla Mustafa, Nahar Sultana, Gazi Nurun, Salhimi, Salizawati Muhamad, and Hossain, Chowdhury Faiz
- Subjects
- *
ZINGIBER , *HEXANE , *COLUMN chromatography , *FRACTIONS , *ASPERGILLUS niger , *LIQUID chromatography - Abstract
The rhizome of Zingiber roseum has been used as traditional medicines for the treatment of gastric ulcer, skin diseases, and liver infections. Zerumbone is an essential sesquiterpenoid previously isolated from different species of Zingiberaceae and possesses numerous pharmacological properties. Based on previous reports, the aim of the current study was to investigate the antimicrobial activity of extract, fractions, and isolation of zerumbone from Zingiber roseum. The antimicrobial activity was determined using disc diffusion method. The serial tube dilution technique was used to determine Minimum Inhibitory Concentration (MIC) of the samples. Vacuum Liquid Chromatography (VLC) and open column chromatography were used for separation. The methanol extract exhibited maximum antimicrobial activity against Escherichia coli, Saccharomyces cerevisiae, and Aspergillus niger in both methods. VLC of the extract yielded five fractions (Fr. 1 to Fr. 5). Fr. 1 exhibited maximum antimicrobial activity compared to the other fractions. Column chromatography of Fr.1 generated 47 fractions. Fraction 1 to 9 were combined and crystallized in n-hexane to isolate zerumbone as the most abundant source (yield 6.5 % from dried MeOH extract). Zerumbone showed good activity against all tested bacteria. The results of our study might validate the ethnobotanical use of Zingiber roseum rhizomes in the treatment of infectious diseases. Moreover, Zingiber roseum could be one of the most abundant sources for the isolation of natural zerumbone to study different pharmacological studies in the field of new drug discovery. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF