1. Interleukin-1 and interleukin-6 gene expression in human monocytes stimulated with <em>Salmonella typhimurium</em> porins.
- Author
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Galdiero, M., de L'Ero, G. Cipollaro, Donnarumma, G., Marcatili, A., and Galdiero, F.
- Subjects
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INTERLEUKINS , *MONOCYTES , *SALMONELLA typhimurium , *CELL culture , *GENE expression , *IMMUNOREGULATION - Abstract
The aim of this study was to verify whether Salmonella typhimurium porins can affect the expression of interleukin-1 (IL-1) and interleukin-6 (IL-6) genes. Human monocytes were treated with porins, and total RNAs were analysed by Northern blotting to evaluate the expression of IL- 1α IL-1β and IL-6 in both treated and untreated cell cultures. Porins induced a significant increase in IL-1 and IL-6 transcripts. This increase was related to the dose of porins, and it peaked 5 hr after treatment. The same results were obtained when polymyxin B was added to the porin preparation to eliminate eventual traces of lipopolysaccharide (LPS) associated with porins. The porins-mediated increase in interleukin transcripts did not require de novo protein synthesis, and it was because of the enhanced half-life of IL-1 and IL-6 mRNAs, rather an increased rate of gene transcription. These data suggest that porins may affect inflammatory and immunological responses by enhancing the expression of cytokine genes. [ABSTRACT FROM AUTHOR]
- Published
- 1995