1. Identification and bioactivity evaluation of twelve previously undescribed depsidone derivatives from Garcinia oligantha.
- Author
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Peng, Xiao-Hui, Chen, Sha, Liu, Xiao-Fan, Yang, Jin-Yuan, Meng, Fan-Zhu, Cao, Hao, Li, Da-Hong, and Hua, Hui-Ming
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CANCER cell migration , *CELL lines , *CYTOTOXINS , *GARCINIA , *LIVER cancer , *CELL migration - Abstract
Phytochemical studies on the leaves and twigs of Garcinia oligantha Merr. led to the isolation of twelve previously undescribed depsidone derivatives (oliganthdepsidones A-L, 1 – 12). Their structures were elucidated by extensive spectroscopic analysis including 1H and 13C NMR, HSQC, HMBC and NOESY along with HRESIMS. The structures of oliganthdepsidones G and J were finally determined using DFT-NMR chemical shift calculations and DP4+ methods. Cytotoxicity test in four human cancer cell lines indicated that oliganthdepsidone F had relatively strong cytotoxic effect against A375 (melanoma), A549 (lung cancer), HepG2 (liver cancer), and MCF-7 (breast cancer) cell lines with IC 50 of 18.71, 15.44, 10.92, and 15.90 μM, respectively. The dose- and time-dependent antiproliferative effects of oliganthdepsidone F on these cell lines were also observed by CCK-8 test. As determined by fluorescent microscopy and flow cytometry in these cell lines, oliganthdepsidone F could promote cell apoptosis, leading to the inhibition of cell proliferation. The results of wound healing assay and transwell assay showed that oliganthdepsidone F could inhibit the migration and invasion of A549 and MCF-7 cell lines in a concentration-dependent manner. [Display omitted] • Twelve previously undescribed depsidones were isolated from Garcinia oligantha. • DFT-NMR calculations and DP4+ methods were used in structure determination. • Oliganthdepsidone F exhibited potent cytotoxicity against A375, A549, HepG2, and MCF-7 cells. • Oliganthdepsidone F induced apoptosis and impeded the migration and invasion of cancer cells. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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