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Copyright of Chinese Journal of Cancer Biotherapy is the property of Editorial Office of Chinese Journal of Cancer Biotherapy and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: The main purpose of this paper was to prepare the immunoactive peptides from Meretrix meretrix Linnaeus (MLIP) by enzymatic hydrolysis, optimize the enzymolysis conditions of MLIP, and studied its immune activity. Method: MLIP was used as raw material, and five commonly used proteases were chosen to hydrolyze. The proliferation rate of RAW264.7 cells was selected as an indicator to screen the most suitable protease for enzymolysis solution. Then, the single factor test was carried out, and three factors with significant impact were selected to optimize the enzymolysis conditions by response surface method, and MLIP was prepared with the optimized process parameters. In addition, this research also studied the effects of different concentrations of MLIP on the relative proliferation rate, cell morphology and NO release of RAW264.7 cells. Results: The proliferation rate results showed that complex protease was the most suitable enzyme to prepare MLIP. The optimal enzymatic parameters obtained by response surface optimization were: pH 6.8, solid-liquid ratio 1:6.2, enzyme dosage 3 493 U/g, enzymolysis at 45 °C for 3 hours. The relative proliferation rate of RAW264.7 cells was predicted to be 71.40%, which were basically the same with the results obtained by verification experiment. In addition, RAW264.7 cells showed the best proliferation promoting effect when the concentration of MLIP was 0.4 mg/mL. All dose of MLIP caused differentiation to varying degrees of cell differentiation and had a certain promoting effect on the release of NO of RAW264.7 cells. Conclusion: The results indicated that the MLIP exhibit desirable immune activity, which might provide reference for the preparation and further study of the MLIP. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Osteoporosis is the property of Chinese Journal of Osteoporosis and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Northwest A & F University - Natural Science Edition is the property of Editorial Department of Journal of Northwest A&F University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Cancer Biotherapy is the property of Editorial Office of Chinese Journal of Cancer Biotherapy and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Magnesium alloys with porous structure are promising for human bone scaffold transplantation due to biodegradability and similar mechanical properties, which is also conducive to the growth, proliferation and differentiation of cells. The preparation of porous magnesium alloy bone scaffolds by Selective Laser Melting(SLM) method in additive manufacturing avoids the morphological and performance defects arising from traditional casting and other processes while precisely controlling porosity and dimensions. This paper presents the feasibility of porous magnesium alloy bone scaffolds for clinical applications and describes the SLM process. The enhancement mechanism is analyzed to clarify the performance advantages of the SLM process for magnesium alloys. The mechanism of action and optimization is also analyzed according to the variation of elements and process parameters. Finally, the bottlenecks of the current process and its factors are summarized, and the directions of improvement and future development trends are proposed to realize its medical industrial applications. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Changzhou University (Natural Science Edition) / Changzhou Daxue Xuebao (Ziran Kexue Ban) is the property of Editorial Department of Journal of Changzhou University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Nanjing Agricultural University / Nanjuing Nongye Daxue Xuebao is the property of Journal of Nanjing Agricultural University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Tianjin Medical Journal is the property of Tianjin Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Tianjin Medical Journal is the property of Tianjin Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of West China Journal of Stomatology is the property of Sichuan University, West China College of Stomatology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Graphene-related materials have good biocompatibility and can improve cartilage repair. At the same time, their excellent mechanical strength and electrical conductivity make them promising as cartilage replacement materials, which have been widely used in tissue engineering. OBJECTIVE: To review the general properties, biocompatibility and application of graphene in cartilage tissue engineering and cartilage repair. METHODS: A computer-based online search of CNKI and PubMed databases was performed using the search terms "graphene, tissue engineering, biocompatibility, cartilage" in Chinese and English to search related literatures published between January 2000 and January 2019. Preliminary screening was conducted by reading the titles and abstracts to exclude the literature irrelevant to the theme of the paper. According to inclusion and exclusion criteria, 57 literatures were included in the final analysis. RESULTS AND CONCLUSION: Graphene has good biocompatibility, and has low cytotoxicity to prokaryotic cells and eukaryotic cells, but the cytotoxicity can be further reduced by chemical modification or surface modification, so as not to affect the growth of cells. Graphene and its derivatives can promote the growth and chondrogenic differentiation of human bone marrow mesenchymal stem cells, as well as the proliferation and maturation of chondrocytes, and accelerate the repair of cartilage defects. Due to its mechanical strength and electrical conductivity, graphene can compound biomimetic cartilage material, which is suitable for cartilage tissue engineering. Graphene has several unresolved problems and challenges, but the application potential of graphene-related materials may pave the way for future breakthroughs in tissue engineering research. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Tianjin Medical Journal is the property of Tianjin Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Osteoporosis is a systemic metabolic disease which is characterized by a decrease in bone mass as well as a deterioration of the bone micro-architecture. It leads to increase of bone fragility and risk of the fracture. Glucocorticoids are widely used for their anti-inflammation and immune modulation effects. However, excess glucocorticoids result in osteoporosis, one of the secondary osteoporosis. Bone marrow stromal cells (BMSCs) have the potential of multi-directional differentiation, from which adipocytes and osteoblasts are originated. Within the bone marrow, the differentiation into adipocytes or osteoblasts is competitively balanced. In normal range, glucocorticoids promote the osteogenic and adipogenic differentiation. During the process, glucocorticoids may combine with many different factors to up-regulate or down-regulate osteogenic differentiation. This paper reviews the mechanism of two-way regulation of BMSCs differentiation by glucocorticoid, and the prevention and treatment of hormone4nduced osteoporosis from maximized osteogenic differentiation point of view. [ABSTRACT FROM AUTHOR]

Nerve growth factor (nerve growth factor, NGF) is the earliest discovered growth factor in neurotrophic factors, it has a very important role in maintaining the survival, growth, differentiation, repair and regeneration after injury of neurons. In this paper, research progress in recent years on NGF are reviewed. [ABSTRACT FROM AUTHOR]

MicroRNAs (miRNAs)play important roles in many cellular events, such as proliferation, differentiation, apoptosis, survive and tumorigenesis by regulating expression of target genes at posttranscriptional level. Many miRNAs have been found in ovary, including miR-23a and miR-27a. miR-23a and miR-27a are members of the miR-23a~27a~24-2 super-family, induce apoptosis via caspase-dependent or caspaseindependent pathways and endoplasmic reticulum stress. It was found that miR-23a and miR-27a were involved in many ovarian function and pathophysiology, including follicular development, hormone synthesis and secretion, premature ovarian failure and ovarian cancer. Many effects of miRNAs in ovarian function have been elucidated, which is helpful for new treatment options for sterility and other ovarian diseases. The functions of miR-23a and miR-27a in ovary were reviewed in this paper. [ABSTRACT FROM AUTHOR]

PURPOSE: To study the effects of surface roughness and composition of titanium on proliferation and differentiation of osteoblasts. METHODS: Osteoblasts were cultured on 5 commercially pure titanium (cp Ti) substrates of ground (S0), blasted with 108~130 |xm(S1), 216~301 m(S2), 356~411 m (S3) TiO2 particles and titanium-sprayed plasma (TPS) surfaces. Surfaces prepared by hand grinding with SiC paper of 600 grits served as control (S0). Electron microprobe was used to evaluate the TiO2 film structure of the 5 titanium surfaces. For proliferation and differentiation measurement, osteoblasts were cultured for 1, 3, 5 and 7 days,evaluated by MTT Assay, ALP activity and OC level. SPSS12.0 software package was used for one-way ANOVA. RESULTS: The number of cells was the highest on S3 and TPS surfaces after 1 day culture. The same results were observed after 5 days. On day 3 and 7, S3 surface was the highest (P<0.05). The number of cells on all experimental groups were higher than S0 surfaces at each time point (P<0.05). Increase of ALP activity were detected on S0, S1 and S2 surfaces after 1, 3, 5 days. However, there was no difference between S3 and TPS surfaces (Pg0.05). After 7 days, ALP activity increased significantly on TPS surface than on S1 or S2 surfaces. ALP activity on S3 surfaces was the highest ( P<0.05) . The ALP activity on all experimental groups were higher than S0 surfaces at each time point ( P<0.05) . An increase in OC production was detected on S0, S1, S2 and S3 surfaces after 1, 3, 5 days. The highest OC production was on S3 surface was on day 7 (P<0.05). CONCLUSIONS: It was concluded that for TiO2 blasted surfaces, Ra ranging from 1.260 m to 3.530 m would optimize proliferation and differentiation of osteoblasts. Blasting was an effective treatment method for osteointegration in vitro. [ABSTRACT FROM AUTHOR]