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The article presents a study which aims to present the existence of guppy population (GP) or Poecilia reticulata in natural thermal waters of Serbia. The biological characteristics and the types of habitat for the GP is provided. The ecological measurement of the river, total body length, and age structure of the guppy population are also stated. It is inferred that low water temperature and high abundance of the predatory fish Leuciscus cephalus are the limiting factors for the GP.