30 results on '"Beres, Stephen B."'
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2. Genetic heterogeneity of the Spy1336/R28—Spy1337 virulence axis in Streptococcus pyogenes and effect on gene transcript levels and pathogenesis.
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Eraso, Jesus M., Kachroo, Priyanka, Olsen, Randall J., Beres, Stephen B., Zhu, Luchang, Badu, Traci, Shannon, Sydney, Cantu, Concepcion C., Saavedra, Matthew Ojeda, Kubiak, Samantha L., Porter, Adeline R., DeLeo, Frank R., and Musser, James M.
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STREPTOCOCCUS pyogenes ,STREPTOCOCCUS ,TANDEM repeats ,TOXIC shock syndrome ,NEONATAL sepsis ,NEONATAL diseases ,GENES - Abstract
Streptococcus pyogenes is a strict human pathogen responsible for more than 700 million infections annually worldwide. Strains of serotype M28 S. pyogenes are typically among the five more abundant types causing invasive infections and pharyngitis in adults and children. Type M28 strains also have an unusual propensity to cause puerperal sepsis and neonatal disease. We recently discovered that a one-nucleotide indel in an intergenic homopolymeric tract located between genes Spy1336/R28 and Spy1337 altered virulence in a mouse model of infection. In the present study, we analyzed size variation in this homopolymeric tract and determined the extent of heterogeneity in the number of tandemly-repeated 79-amino acid domains in the coding region of Spy1336/R28 in large samples of strains recovered from humans with invasive infections. Both repeat sequence elements are highly polymorphic in natural populations of M28 strains. Variation in the homopolymeric tract results in (i) changes in transcript levels of Spy1336/R28 and Spy1337 in vitro, (ii) differences in virulence in a mouse model of necrotizing myositis, and (iii) global transcriptome changes as shown by RNAseq analysis of isogenic mutant strains. Variation in the number of tandem repeats in the coding sequence of Spy1336/R28 is responsible for size variation of R28 protein in natural populations. Isogenic mutant strains in which genes encoding R28 or transcriptional regulator Spy1337 are inactivated are significantly less virulent in a nonhuman primate model of necrotizing myositis. Our findings provide impetus for additional studies addressing the role of R28 and Spy1337 variation in pathogen-host interactions. [ABSTRACT FROM AUTHOR]
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- 2020
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3. Novel Genes Required for the Fitness of Streptococcus pyogenes in Human Saliva.
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Luchang Zhu, Charbonneau, Amelia R. L., Waller, Andrew S., Olsen, Randall J., Beres, Stephen B., and Musser, James M.
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- 2017
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4. Rapid Emergence of a New Clone Impacts the Population at Risk and Increases the Incidence of Type emm89 Group A Streptococcus Invasive Disease.
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Teatero, Sarah, Coleman, Brenda L., Beres, Stephen B., Olsen, Randall J., Kandel, Christopher, Reynolds, Olivia, Athey, Taryn B. T., Musser, James M., McGeer, Allison, and Fittipaldi, Nahuel
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STREPTOCOCCAL diseases ,DISEASE incidence ,STREPTOCOCCUS - Abstract
Background. Invasive group A Streptococcus (iGAS) disease caused by type emm89 strains has been increasing worldwide, driven by the emergence of an epidemic clonal variant (clade 3 emm89). The clinical characteristics of patients with emm89 iGAS disease, and in particular with clade 3 emm89 iGAS disease, are poorly described. Methods. We used population-based iGAS surveillance data collected in metropolitan Toronto, Ontario, Canada during the period 2000-2014. We sequenced the genomes of 105 emm89 isolates representing all emm89 iGAS disease cases in the area during the period and 138 temporally matched emm89 iGAS isolates collected elsewhere in Ontario. Results. Clades 1 and 2 and clade O, a newly discovered emm89 genetic variant, caused most cases of emm89 iGAS disease in metropolitan Toronto before 2008. After rapid emergence of new clade 3, previously circulating clades were purged from the population and the incidence of emm89 iGAS disease significantly increased from 0.14 per 100 000 in 2000-2007 to 0.22 per 100 000 in 2008-2014. Overall, emm89 organisms caused significantly more arthritis but less necrotizing fasciitis than strains of the more common type emm1. Other clinical presentations were soft tissue and severe respiratory tract infections. Clinical outcomes did not differ significantly between emm89 clades overall. However, clade 3 emm89 iGAS disease was more common in youth and middle-aged individuals. Conclusions. The rapid shift in emm89 iGAS strain genetics in metropolitan Toronto has resulted in a significant increase in the incidence of emm89 iGAS disease, with noticeably higher rates of clade 3 disease in younger patients. [ABSTRACT FROM AUTHOR]
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- 2017
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5. Interacting networks of resistance, virulence and core machinery genes identified by genome-wide epistasis analysis.
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Skwark, Marcin J., Croucher, Nicholas J., Puranen, Santeri, Chewapreecha, Claire, Pesonen, Maiju, Xu, Ying Ying, Turner, Paul, Harris, Simon R., Beres, Stephen B., Musser, James M., Parkhill, Julian, Bentley, Stephen D., Aurell, Erik, and Corander, Jukka
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METAGENOMICS ,MICROBIAL virulence ,STREPTOCOCCUS pneumoniae ,BACTERIAL genetics ,EPISTASIS (Genetics) ,DRUG resistance in microorganisms - Abstract
Recent advances in the scale and diversity of population genomic datasets for bacteria now provide the potential for genome-wide patterns of co-evolution to be studied at the resolution of individual bases. Here we describe a new statistical method, genomeDCA, which uses recent advances in computational structural biology to identify the polymorphic loci under the strongest co-evolutionary pressures. We apply genomeDCA to two large population data sets representing the major human pathogens Streptococcus pneumoniae (pneumococcus) and Streptococcus pyogenes (group A Streptococcus). For pneumococcus we identified 5,199 putative epistatic interactions between 1,936 sites. Over three-quarters of the links were between sites within the pbp2x, pbp1a and pbp2b genes, the sequences of which are critical in determining non-susceptibility to beta-lactam antibiotics. A network-based analysis found these genes were also coupled to that encoding dihydrofolate reductase, changes to which underlie trimethoprim resistance. Distinct from these antibiotic resistance genes, a large network component of 384 protein coding sequences encompassed many genes critical in basic cellular functions, while another distinct component included genes associated with virulence. The group A Streptococcus (GAS) data set population represents a clonal population with relatively little genetic variation and a high level of linkage disequilibrium across the genome. Despite this, we were able to pinpoint two RNA pseudouridine synthases, which were each strongly linked to a separate set of loci across the chromosome, representing biologically plausible targets of co-selection. The population genomic analysis method applied here identifies statistically significantly co-evolving locus pairs, potentially arising from fitness selection interdependence reflecting underlying protein-protein interactions, or genes whose product activities contribute to the same phenotype. This discovery approach greatly enhances the future potential of epistasis analysis for systems biology, and can complement genome-wide association studies as a means of formulating hypotheses for targeted experimental work. [ABSTRACT FROM AUTHOR]
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- 2017
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6. Rapid Emergence of a New Clone Impacts the Population at Risk and Increases the Incidence of Type emm89 Group A Streptococcus Invasive Disease.
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Teatero, Sarah, Coleman, Brenda L., Beres, Stephen B., Olsen, Randall J., Kande, Christopher, Reynolds, Olivia, Athey, Taryn B. T., Musser, James M., McGeer, Allison, and Fittipaldi, Nahuel
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NECROTIZING fasciitis ,CLONAL variation (Plants) ,PUBLIC health - Abstract
Background. Invasive group A Streptococcus (iGAS) disease caused by type emm89 strains has been increasing worldwide, driven by the emergence of an epidemic clonal variant (clade 3 emm89). The clinical characteristics of patients with emm89 iGAS disease, and in particular with clade 3 emm89 iGAS disease, are poorly described. Methods. We used population-based iGAS surveillance data collected in metropolitan Toronto, Ontario, Canada during the period 2000-2014. We sequenced the genomes of 105 emm89 isolates representing all emm89 iGAS disease cases in the area during the period and 138 temporally matched emm89 iGAS isolates collected elsewhere in Ontario. Results. Clades 1 and 2 and clade O, a newly discovered emm89 genetic variant, caused most cases of emm89 iGAS disease in metropolitan Toronto before 2008. After rapid emergence of new clade 3, previously circulating clades were purged from the population and the incidence of emm89 iGAS disease significantly increased from 0.14 per 100 000 in 2000-2007 to 0.22 per 100 000 in 2008-2014. Overall, emm89 organisms caused significantly more arthritis but less necrotizing fasciitis than strains of the more common type emm1. Other clinical presentations were soft tissue and severe respiratory tract infections. Clinical outcomes did not differ significantly between emm89 clades overall. However, clade 3 emm89 iGAS disease was more common in youth and middle-aged individuals. Conclusions. The rapid shift in emm89 iGAS strain genetics in metropolitan Toronto has resulted in a significant increase in the incidence of emm89 iGAS disease, with noticeably higher rates of clade 3 disease in younger patients. [ABSTRACT FROM AUTHOR]
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- 2017
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7. Genomic Characteristics Behind the Spread of Bacteremic Group A Streptococcus Type emm89 in Finland, 2004-2014.
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Latronico, Francesca, Nasser, Waleed, Puhakainen, Kai, Ollgren, Jukka, Hyyryläinen, Hanne-Leena, Beres, Stephen B., Lyytikäinen, Outi, Jalava, Jari, Musser, James M., and Vuopio, Jaana
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STREPTOCOCCACEAE ,STREPTOCOCCUS ,GENOMICS ,PATHOGENIC microorganisms ,LOGISTIC regression analysis - Abstract
Background: Many countries worldwide have reported increasing numbers of emm89 group A Streptococcus (GAS) infections during last decade. Pathogen genetic factors linked to this increase need assessment.Methods: We investigated epidemiological characteristics of emm89 GAS bacteremic infections, including 7-day and 30-day case-fatality rates, in Finland during 2004-2014 and linked them to whole-genome sequencing data obtained from corresponding strains. The Fisher exact test and exact logistic regression were used to compare differences between bacteremic infections due to emm89 GAS belonging to different genetic clades and subclades.Results: Out of 1928 cases of GAS bacteremic infection, 278 were caused by emm89 GAS. We identified 2 genetically distinct clades, arbitrarily designated clade 2 and clade 3. Both clades were present during 2004-2008, but clade 3 increased rapidly from 2009 onward. Six subclades (designated subclades A-F) were identified within clade 3, based on phylogenetic core genome analysis. The case-fatality rate differed significantly between subclades (P < .05), with subclade D having the highest 30-day estimated case-fatality rate (19% vs 3%-14%).Conclusions: A new emm89 clone, clade 3, emerged in 2009 and spread rapidly in Finland. Patients infected with certain subclades of clade 3 were significantly more likely to die. A specific polymerase chain reaction assay was developed to follow the spread of subclade D in 2015. [ABSTRACT FROM AUTHOR]- Published
- 2016
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8. Streptolysin O Rapidly Impairs Neutrophil Oxidative Burst and Antibacterial Responses to Group A Streptococcus.
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Uchiyama, Satoshi, Döhrmann, Simon, Timmer, Anjuli M., Dixit, Neha, Ghochani, Mariam, Bhandari, Tamara, Timmer, John C., Sprague, Kimberly, Bubeck-Wardenburg, Juliane, Simon, Scott I., Nizet, Victor, Alonzo, Francis, and Beres, Stephen B.
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STREPTOCOCCUS ,PHARYNGITIS ,TOXIC shock syndrome - Abstract
Group A Streptococcus (GAS) causes a wide range of human infections, ranging from simple pharyngitis to life-threatening necrotizing fasciitis and toxic shock syndrome. A globally disseminated clone of M1T1 GAS has been associated with an increase in severe, invasive GAS infections in recent decades. The secreted GAS pore-forming toxin streptolysin O (SLO), which induces eukaryotic cell lysis in a cholesterol-dependent manner, is highly upregulated in the GAS M1T1 clone during bloodstream dissemination. SLO is known to promote GAS resistance to phagocytic clearance by neutrophils, a critical first element of host defense against invasive bacterial infection. Here, we examine the role of SLO in modulating specific neutrophil functions during their early interaction with GAS. We find that SLO at subcytotoxic concentrations and early time points is necessary and sufficient to suppress neutrophil oxidative burst, in a manner reversed by free cholesterol and anti-SLO blocking antibodies. In addition, SLO at subcytotoxic concentrations blocked neutrophil degranulation, interleukin-8 secretion and responsiveness, and elaboration of DNA-based neutrophil extracellular traps, cumulatively supporting a key role for SLO in GAS resistance to immediate neutrophil killing. A non-toxic SLO derivate elicits protective immunity against lethal GAS challenge in a murine infection model. We conclude that SLO exerts a novel cytotoxic-independent function at early stages of invasive infections (<30 min), contributing to GAS escape from neutrophil clearance. [ABSTRACT FROM AUTHOR]
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- 2016
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9. Streptolysin O Rapidly Impairs Neutrophil Oxidative Burst and Antibacterial Responses to Group A Streptococcus.
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Satoshi Uchiyama, Döhrmann, Simon, Timmer, Anjuli M., Dixit, Neha, Ghochani, Mariam, Bhandari, Tamara, Timmer, John C., Sprague, Kimberly, Bubeck-Wardenburg, Juliane, Simon, Scott I., Nizet, Victor, Alonzo, Francis, and Beres, Stephen B.
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STREPTOCOCCUS pyogenes ,NEUTROPHILS - Abstract
Group A Streptococcus (GAS) causes a wide range of human infections, ranging from simple pharyngitis to life-threatening necrotizing fasciitis and toxic shock syndrome. A globally disseminated clone of M1T1 GAS has been associated with an increase in severe, invasive GAS infections in recent decades. The secreted GAS pore-forming toxin streptolysin O (SLO), which induces eukaryotic cell lysis in a cholesterol-dependent manner, is highly upregulated in the GAS M1T1 clone during bloodstream dissemination. SLO is known to promote GAS resistance to phagocytic clearance by neutrophils, a critical first element of host defense against invasive bacterial infection. Here, we examine the role of SLO in modulating specific neutrophil functions during their early interaction with GAS. We find that SLO at subcytotoxic concentrations and early time points is necessary and sufficient to suppress neutrophil oxidative burst, in a manner reversed by free cholesterol and anti-SLO blocking antibodies. In addition, SLO at subcytotoxic concentrations blocked neutrophil degranulation, interleukin-8 secretion and responsiveness, and elaboration of DNA-based neutrophil extracellular traps, cumulatively supporting a key role for SLO in GAS resistance to immediate neutrophil killing. A non-toxic SLO derivate elicits protective immunity against lethal GAS challenge in a murine infection model. We conclude that SLO exerts a novel cytotoxic-independent function at early stages of invasive infections (<30 min), contributing to GAS escape from neutrophil clearance. [ABSTRACT FROM AUTHOR]
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- 2015
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10. Comparative Whole Genome Sequencing of Community- Associated Methicillin-Resistant Staphylococcus aureus Sequence Type 8 from Primary Care Clinics in a Texas Community.
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Lee, Grace C., Long, S. Wesley, Musser, James M., Beres, Stephen B., Olsen, Randall J., Dallas, Steven D., Nunez, Yury O., and Frei, Christopher R.
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METHICILLIN-resistant staphylococcus aureus ,GENOMES ,DISPENSARIES ,PENICILLIN ,PATIENTS - Abstract
STUDY OBJECTIVE Our understanding of the molecular dynamics driving the community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) epidemic at the whole genome level is limited. We sought to assess the use of whole genome sequencing (WGS) to evaluate the genomic diversity and genotypic prediction of antimicrobial resistance of CA-MRSA isolates from patients in South Texas. DESIGN Comparative whole genome sequencing. ISOLATES Thirteen clinical CA-MRSA isolates recovered from patients presenting with skin and soft tissue infections to nine primary care clinics in the South Texas Ambulatory Research Network between 2010 and 2013. MEASUREMENTS AND MAIN RESULTS Comparative WGS was performed on the 13 MRSA sequence type 8 clinical isolates. We compared the resistome of genes encoding for antibiotic resistance with a phenotypically derived antibiogram using standard antimicrobial susceptibility testing. The strains differed by an average of 72 single nucleotide polymorphisms (SNPs) per isolate in the core genome compared with FPR3757 (USA300, reference strain). There were a total of 623 unique SNPs in the core genome (range 47-88 SNPs per isolate). We identified 19 nonsynonymous SNPs in genes encoding proven or putative S. aureus virulence determinants in the core genome. There was complete concordance between genotypic evidence for antimicrobial resistance and the phenotypically derived antibiogram. CONCLUSION Overall, although these CA-MRSA isolates were similar on the level of clonal type, clinical syndrome, and geographic area, the strains were diverse at the genome level. Furthermore, our findings provide important proof-of-concept information for using WGS as a potential front-end screening tool for antimicrobial resistance predictions. [ABSTRACT FROM AUTHOR]
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- 2015
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11. III: EVOLUTION OF SELECTED PATHOGENIC SPECIES AND MECHANISMS: CHAPTER 14: GROUP A Streptococcus AND Staphylococcus aureus: EVOLUTION, REEMERGENCE, AND STRAIN DIVERSIFICATION.
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Reid, Sean D., Fitzgerald, J. Ross, Beres, Stephen B., Green, Nicole M., and Musser, James M.
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- 2006
12. Chapter 16: CONTRIBUTION OF PHAGES TO GROUP A STREPTOCOCCUS GENETIC DIVERSITY AND PATHOGENESIS.
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Banks, David J., Beres, Stephen B., and Musser, James M.
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- 2005
13. High-throughput RNA sequencing of a formalin-fixed, paraffin-embedded autopsy lung tissue sample from the 1918 influenza pandemic.
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Xiao, Yong‐Li, Kash, John C, Beres, Stephen B, Sheng, Zong‐Mei, Musser, James M, and Taubenberger, Jeffery K
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Most biopsy and autopsy tissues are formalin-fixed and paraffin-embedded ( FFPE), but this process leads to RNA degradation that limits gene expression analysis. The RNA genome of the 1918 pandemic influenza virus was previously determined in a 9-year effort by overlapping RT-PCR from post-mortem samples. Here, the full genome of the 1918 virus at 3000× coverage was determined in one high-throughput sequencing run of a library derived from total RNA of a 1918 FFPE sample after duplex-specific nuclease treatments. Bacterial sequences associated with secondary bacterial pneumonias were also detected. Host transcripts were well represented in the library. Compared to a 2009 pandemic influenza virus FFPE post-mortem library, the 1918 sample showed significant enrichment for host defence and cell death response genes, concordant with prior animal studies. This methodological approach should assist in the analysis of FFPE tissue samples isolated over the past century from a variety of diseases. [ABSTRACT FROM AUTHOR]
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- 2013
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14. Polymorphisms in Regulator of Protease B (RopB) Alter Disease Phenotype and Strain Virulence of Serotype M3 Group A Streptococcus.
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Olsen, Randall J., Laucirica, Daniel R., Watkins, M. Ebru, Feske, Marsha L., Garcia-Bustillos, Jesus R., Vu, Chau, Cantu, Concepcion, Shelburne, Samuel A., Fittipaldi, Nahuel, Kumaraswami, Muthiah, Shea, Patrick R., Flores, Anthony R., Beres, Stephen B., Lovgren, Maguerite, Tyrrell, Gregory J., Efstratiou, Androulla, Low, Donald E., Van Beneden, Chris A., and Musser, James M.
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GENETIC polymorphisms ,PROTEOLYTIC enzymes ,PHENOTYPES ,MICROBIAL virulence ,SEROTYPES ,STREPTOCOCCUS ,GENOMES - Abstract
Whole-genome sequencing of serotype M3 group A streptococci (GAS) from oropharyngeal and invasive infections in Ontario recently showed that the gene encoding regulator of protease B (RopB) is highly polymorphic in this population. To test the hypothesis that ropB is under diversifying selective pressure among all serotype M3 GAS strains, we sequenced this gene in 1178 strains collected from different infection types, geographic regions, and time periods. The results confirmed our hypothesis and discovered a significant association between mutant ropB alleles, decreased activity of its major regulatory target SpeB, and pharyngitis. Additionally, isoallelic strains with ropB polymorphisms were significantly less virulent in a mouse model of necrotizing fasciitis. These studies provide a model strategy for applying whole-genome sequencing followed by deep single-gene sequencing to generate new insight to the rapid evolution and virulence regulation of human pathogens. [ABSTRACT FROM AUTHOR]
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- 2012
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15. Group A Streptococcus emm Gene Types in Pharyngeal Isolates, Ontario, Canada, 2002-2010.
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Shea, Patrick R., Ewbank, Amy L., Gonzalez-Lugo, Javier H., Martagon-Rosado, Alexandro J., Martinez-Gutierrez, Juan C., Rehman, Hina A., Serrano-Gonzalez, Monica, Fittipaldi, Nahuel, Beres, Stephen B., Flores, Anthony R., Low, Donald E., Willey, Barbara M., and Musser, James M.
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STREPTOCOCCUS ,PHARYNGITIS ,INFECTION ,NUCLEOTIDE sequence ,MEDICAL genetics ,EPIDEMIOLOGY - Abstract
Group A Streptococcus (GAS) is a human-adapted pathogen that causes a variety of diseases, including pharyngitis and invasive infections. GAS strains are categorized by variation in the nucleotide sequence of the gene (emm) that encodes the M protein. To identify the emm types of GAS strains causing pharyngitis in Ontario, Canada, we sequenced the hypervariable region of the emm gene in 4,635 pharyngeal GAS isolates collected during 2002-2010. The most prevalent emm types varied little from year to year. In contrast, fine-scale geographic analysis identified inter-site variability in the most common emm types. Additionally, we observed fluctuations in yearly frequency of emm3 strains from pharyngitis patients that coincided with peaks of emm3 invasive infections. We also discovered a striking increase in frequency of emm89 strains among isolates from patients with pharyngitis and invasive disease. These findings about the epidemiology of GAS are potentially useful for vaccine research. [ABSTRACT FROM AUTHOR]
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- 2011
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16. Distinct signatures of diversifying selection revealed by genome analysis of respiratory tract and invasive bacterial populations.
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Shea, Patrick R., Beres, Stephen B., Flores, Anthony R., Ewbank, Amy L., Gonzalez-Lugo, Javier H., Martagon-Rosado, Alexandro J., Martinez-Gutierrez, Juan C., Rehman, Hina A., Serrano-Gonzalez, Monica, Fittipaldi, Nahuel, Ayers, Stephen D., Webb, Paul, Willey, Barbara M., Low, Donald E., and Musser, James M.
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PATHOGENIC microorganisms ,GENETICS of bacterial diversity ,BIODIVERSITY ,MICROBIAL genetics ,GENOMICS - Abstract
Many pathogens colonize different anatomical sites, but the selective pressures contributing to survival in the diverse niches are poorly understood. Group A Streptococcus (GAS) is a human-adapted bacterium that causes a range of infections. Much effort has been expended to dissect the molecular basis of invasive (sterile-site) infections, but little is known about the genomes of strains causing pharyngitis (streptococcal "sore throat"). Additionally, there is essentially nothing known about the genetic relationships between populations of invasive and pharyngitis strains. In particular, it is unclear if invasive strains represent a distinct genetic subpopulation of strains that cause pharyngitis. We compared the genomes of 86 serotype M3 GAS pharyngitis strains with those of 215 invasive M3 strains from the same geographical location. The pharyngitis and invasive groups were highly related to each other and had virtually identical phylogenetic structures, indicating they belong to the same genetic pool. Despite the overall high degree of genetic similarity, we discovered that strains from different host environments (i.e., throat, normally sterile sites) have distinct patterns of diversifying selection at the nucleotide level. In particular, the pattern of polymorphisms in the hyaluronic acid capsule synthesis operon was especially different between the two strain populations. This finding was mirrored by data obtained from full-genome analysis of strains sequentially cultured from nonhuman primates. Our results answer the long-standing question of the genetic relationship between GAS pharyngitis and invasive strains. The data provide previously undescribed information about the evolutionary history of pathogenic microbes that cause disease in different anatomical sites. [ABSTRACT FROM AUTHOR]
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- 2011
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17. Virulence of serotype M3 Group A Streptococcus strains in wax worms (Galleria mellonella larvae).
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Olsen, Randall J., Watkins, M. Ebru, Cantu, Concepcion C., Beres, Stephen B., and Musser, James M.
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- 2011
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18. Molecular complexity of successive bacterial epidemics deconvoluted by comparative pathogenomics.
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Beres, Stephen B., CarrolI, Ronan K., Shea, Patrick R., Sitkiewicz, Izabela, Martinez-Gutierrez, Juan Carlos, Low, Donald E., McGeer, Allison, Willey, Barbara M., Green, Karen, Tyrrell, Gregory J., GoIdman, Thomas D., Michael Feldgarden, Birren, Bruce W., Fofanov, Yuriy, Boos, John, Wheaton, William D., Honisch, Christiane, and Musser, James M.
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NUCLEOTIDE sequence ,COMMUNICABLE diseases ,EPIDEMICS ,STREPTOCOCCUS -- Molecular aspects ,MASS spectrometry ,BACTERIAL genomes ,BACTERIAL diseases - Abstract
Understanding the fine-structure molecular architecture of bacterial epidemics has been a long-sought goal of infectious disease research. We used short-read-length DNA sequencing coupled with mass spectroscopy analysis of SNPs to study the molecular pathogenomics of three successive epidemics of invasive infections involving 344 serotype M3 group A Streptococcus in Ontario, Canada. Sequencing the genome of 95 strains from the three epidemics, coupled with analysis of 280 biallelic SNPs in all 344 strains, revealed an unexpectedly complex population structure composed of a dynamic mixture of distinct clonally related complexes. We discovered that each epidemic is dominated by microand macrobursts of multiple emergent clones, some with distinct strain genotype-patient phenotype relationships. On average, strains were differentiated from one another by only 49 SNPs and 11 insertion-deletion events (indels) in the core genome. Ten percent of SNPs are strain specific; that is. each strain has a unique genome sequence. We identified nonrandom temporal-spatial patterns of strain distribution within and between the epidemic peaks. The extensive full-genome data. permitted us to identify genes with significantly increased rates of nonsynonymous (amino acid-altering) nucleotide polymorphisms, thereby providing dues about selective forces operative in the host. Comparative expression microarray analysis revealed that closely related strains differentiated by seemingly modest genetic changes can have significantly divergent transcriptomes. We conclude that enhanced understanding of bacterial epidemics requires a deep-sequencing, geographically centric, comparative pathogenomics strategy. [ABSTRACT FROM AUTHOR]
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- 2010
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19. Decreased necrotizing fasciitis capacity caused by a single nucleotide mutation that alters a multiple gene virulence axis.
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Olsen, Randall J., Sitkiewicz, Izabela, Ayeras, Ara A., Gonulal, Vedia E., Cantu, Concepcion, Beres, Stephen B., Green, Nicole M., Lei, Benfang, Humbird, Tammy, Greaver, Jamieson, Chang, Ellen, Ragasa, Willie P., Montgomery, Charles A., Cartwright, Jr., Joiner, McGeer, Allison, Low, Donald E., Whitney, Adeline R., Cagle, Philip T., BlasdeI, Terry L., and DeLeo, Frank R.
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NECROTIZING fasciitis ,NUCLEOTIDES ,GENETIC mutation ,MICROBIAL virulence ,STREPTOCOCCUS ,MOLECULAR chaperones ,PROTEOLYTIC enzymes ,PHENOTYPES - Abstract
Single-nucleotide changes are the most common cause of natural genetic variation among members of the same species, but there is remarkably little information bearing on how they alter bacterial virulence. We recently discovered a single-nucleotide mutation in the group A Streptococcus genome that is epidemiologically associated with decreased human necrotizing fasciitis ("flesh-eating disease"). Working from this clinical observation, we find that wild-type mtsR function is required for group A Streptococcus to cause necrotizing fasciitis in mice and nonhuman primates. Expression microarray analysis revealed that mtsR inactivation results in overexpression of PrsA, a chaperonin involved in posttranslational maturation of SpeB, an extracellular cysteine protease. Isogenic mutant strains that overexpress prsA or lack speB had decreased secreted protease activity in vivo and recapitulated the necrotizing fasciitis-negative phenotype of the ΔmtsR mutant strain in mice and monkeys. mtsR inativation results in increased PrsA expression, which in turn causes decreased SpeB secreted protease activity and reduced necrotizing fasciitis capacity. Thus, a naturally occurring single-nucleotide mutation dramatically alters virulence by dysregulating a multiple gene virulence axis. Our discovery has broad implications for the confluence of population genomics and molecular pathogenesis research. [ABSTRACT FROM AUTHOR]
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- 2010
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20. Contribution of AmyA, an extracellular α-glucan degrading enzyme, to group A streptococcal host–pathogen interaction.
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Shelburne III, Samuel A., Keith, David B., Davenport, Michael T., Beres, Stephen B., Carroll, Ronan K., and Musser, James M.
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GLUCANS ,STARCH ,GLYCOGEN ,STREPTOCOCCUS ,PATHOGENIC microorganisms ,ENZYMES ,GENOMES - Abstract
α-Glucans such as starch and glycogen are abundant in the human oropharynx, the main site of group A Streptococcus (GAS) infection. However, the role in pathogenesis of GAS extracellular α-glucan binding and degrading enzymes is unknown. The serotype M1 GAS genome encodes two extracellular proteins putatively involved in α-glucan binding and degradation; pulA encodes a cell wall anchored pullulanase and amyA encodes a freely secreted putative cyclomaltodextrin α-glucanotransferase. Genetic inactivation of amyA, but not pulA, abolished GAS α-glucan degradation. The Δ amyA strain had a slower rate of translocation across human pharyngeal epithelial cells. Consistent with this finding, the Δ amyA strain was less virulent following mouse mucosal challenge. Recombinant AmyA degraded α-glucans into β-cyclomaltodextrins that reduced pharyngeal cell transepithelial resistance, providing a physiologic explanation for the observed transepithelial migration phenotype. Higher amyA transcript levels were present in serotype M1 GAS strains causing invasive infection compared with strains causing pharyngitis. GAS proliferation in a defined α-glucan-containing medium was dependent on the presence of human salivary α-amylase. These data delineate the molecular mechanisms by which α-glucan degradation contributes to GAS host–pathogen interaction, including how GAS uses human salivary α-amylase for its own metabolic benefit. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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21. Genome Sequence of a Lancefield Group C Streptococcus zooepidemicus Strain Causing Epidemic Nephritis: New Information about an Old Disease.
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Beres, Stephen B., Sesso, Ricardo, Pinto, Sergio Wyton L., Hoe, Nancy P., Porcella, Stephen F., DeLeo, Frank R., and Musser, James M.
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HOST-parasite relationships ,STREPTOCOCCUS ,NUCLEOTIDE sequence ,GENOMES ,INTERSTITIAL nephritis - Abstract
Outbreaks of disease attributable to human error or natural causes can provide unique opportunities to gain new information about host-pathogen interactions and new leads for pathogenesis research. Poststreptococcal glomerulonephritis (PSGN), a sequela of infection with pathogenic streptococci, is a common cause of preventable kidney disease worldwide. Although PSGN usually occurs after infection with group A streptococci, organisms of Lancefield group C and G also can be responsible. Despite decades of study, the molecular pathogenesis of PSGN is poorly understood. As a first step toward gaining new information about PSGN pathogenesis, we sequenced the genome of Streptococcus equi subsp. zooepidemicus strain MGCS10565, a group C organism that caused a very large and unusually severe epidemic of nephritis in Brazil. The genome is a circular chromosome of 2,024,171 bp. The genome shares extensive gene content, including many virulence factors, with genetically related group A streptococci, but unexpectedly lacks prophages. The genome contains many apparently foreign genes interspersed around the chromosome, consistent with the presence of a full array of genes required for natural competence. An inordinately large family of genes encodes secreted extracellular collagen-like proteins with multiple integrin-binding motifs. The absence of a gene related to speB rules out the long-held belief that streptococcal pyrogenic exotoxin B or antibodies reacting with it singularly cause PSGN. Many proteins previously implicated in GAS PSGN, such as streptokinase, are either highly divergent in strain MGCS10565 or are not more closely related between these species than to orthologs present in other streptococci that do not commonly cause PSGN. Our analysis provides a comparative genomics framework for renewed appraisal of molecular events underlying APSGN pathogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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22. Molecular genetic anatomy of inter- and intraserotype variation in the human bacterial pathogen group A Streptococcus.
- Author
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Beres, Stephen B., Richter, Ellen W., Nagiec, Michal J., Sumby, Paul, Porcella, Stephen F., DeLeo, Frank R., and Musser, James M.
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PHENOTYPES ,MOLECULAR genetics ,GENOMES ,GENETICS ,GENES ,MOLECULAR biology ,PATHOGENIC microorganisms - Abstract
In recent years we have studied the relationship between strain genotypes and patient phenotypes in group A Streptococcus (GAS), a model human bacterial pathogen that causes extensive morbidity and mortality worldwide. We have concentrated our efforts on serotype M3 organisms because these strains are common causes of pharyngeal and invasive infections, produce unusually severe invasive infections, and can exhibit epidemic behavior. Our studies have been hindered by the lack of genome-scale phylogenies of multiple GAS strains and whole-genome sequences of multiple serotype M3 strains recovered from individuals with defined clinical phenotypes. To remove some of these impediments, we sequenced to closure the genome of four additional GAS strains and conducted comparative genomic resequencing of 12 contemporary serotype M3 strains representing distinct genotypes and phenotypes. Serotype M3 strains are a single phylogenetic lineage. Strains from asymptomatic throat carriers were significantly less virulent for mice than sterile-site isolates and evolved to a less virulent phenotype by multiple genetic pathways. Strain persistence or extinction between epidemics was strongly associated with presence or absence, respectively, of the prophage encoding streptococcal pyrogenic exotoxin A. A serotype M3 clone significantly underrepresented among necrotizing fasciitis cases has a unique frameshift mutation that truncates MtsR, a transcriptional regulator controlling expression of genes encoding iron-acquisition proteins. Expression microarray analysis of this clone confirmed significant alteration in expression of genes encoding iron metabolism proteins. Our analysis provided unprecedented detail about the molecular anatomy of bacterial strain genotype-patient phenotype relationships. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
23. Genome Sequence of a Serotype M28 Strain of Group A Streptococcus: Potential New Insights into Puerperal Sepsis and Bacterial Disease Specificity.
- Author
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Green, Nicole M., Shizhen Zhang, Porcella, Stephen F., Nagiec, Michal J., Barbian, Kent O., Beres, Stephen B., LeFebvre, Rance B., and Musser, James M.
- Subjects
PUERPERAL septicemia ,PATHOGENIC microorganisms ,GRAM-positive bacteria ,STREPTOCOCCUS ,MICROBIAL virulence ,HOST-parasite relationships - Abstract
Puerperal sepsis, a major cause of death of young women in Europe in the 1800s, was due predominantly to the gram-positive pathogen group A Streptococcus. Studies conducted during past decades have shown that serotype M28 strains are the major group A Streptococcus organisms responsible for many of these infections. To begin to increase our understanding of their enrichment in puerperal sepsis, we sequenced the genome of a genetically representative strain. This strain has genes encoding a novel array of prophage virulence factors, cell-surface proteins, and other molecules likely to contribute to host-pathogen interactions. Importantly, genes for 7 inferred extracellular proteins are encoded by a 37.4-kb foreign DNA element that is shared with group B Streptococcus and is present in all serotype M28 strains. Proteins encoded by the 37.4-kb element were expressed extracellularly and in human infections. Acquisition of foreign genes has helped create a disease-specialist clone of this pathogen. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
24. Progress toward Characterization of the Group A Streptococcus Metagenome: Complete Genome Sequence of a Macrolide-Resistant Serotype M6 Strain.
- Author
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Banks, David J., Porcella, Stephen F., Barbian, Kent D., Beres, Stephen B., Philips, Lauren E., Voyich, Jovanka M., Deleo, Frank R., Martin, Judith M., Somerville, Greg A., and Musser, James M.
- Subjects
GENOMES ,BLOOD plasma ,NUCLEOTIDE sequence ,MACROLIDE antibiotics ,STREPTOCOCCUS ,SEROLOGY - Abstract
We describe the genome sequence of a macrolide-resistant strain (MGAS 10394) of serotype M6 group A Streptococcus (GAS). The genome is 1,900,156 bp in length, and 8 prophage-like elements or remnants compose 12.4% of the chromosome. A 8.3-kb prophage remnant encodes the SpeA4 variant of streptococcal pyrogenic exotoxin A. The genome of strain MGA510394 contains a chimeric genetic element composed of prophage genes and a transposon encoding the mefA gene conferring macrolide resistance. This chimeric element also has a gene encoding a novel surface-exposed protein (designated "R6 protein"), with an LPKTG cell-anchor motif located at the carboxyterminus. Surface expression of this protein was confirmed by flow cytometry. Humans with GAS pharyngitis caused by serotype M6 strains had antibody against the R6 protein present in convalescent, but not acute, serum samples. Our studies add to the theme that GAS prophage-encoded extracellular proteins contribute to host-pathogen interactions in a strain-specific fashion. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
25. Genome-wide molecular dissection of serotype M3 group A Streptococcus strains causing two epidemics of invasive infections.
- Author
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Beres, Stephen B., Sylva, Gail L., Sturdevant, Daniel E., Granville, Chanel N., Liu, Mengyao, Ricklefs, Stacy M., Whitney, Adeline R., Parkins, Larye D., Hoe, Nancy P., Adams, Gerald J., Low, Donald E., Deleo, Frank R., McGeer, Allison, and Musser, James M.
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GENETIC polymorphisms ,COMMUNICABLE diseases ,EPIDEMICS ,GENOMES ,EPIDEMIOLOGY ,INFECTION ,COLLOIDS ,PREVENTIVE medicine ,VACCINATION - Abstract
Molecular factors that contribute to the emergence of new virulent bacterial subclones and epidemics are poorly understood. We hypothesized that analysis of a population-based strain sample of serotype M3 group A Streptococcus (GAS) recovered from patients with invasive infection by using genome-wide investigative methods would provide new insight into this fundamental infectious disease problem. Serotype M3 GAS strains (n = 255) cultured from patients in Ontario, Canada, over 11 years and representing two distinct infection peaks were studied. Genetic diversity was indexed by pulsed-field gel electrophoresis, DNA-DNA microarray, whole-genome PCR scanning, prophage genotyping, targeted gene sequencing, and single-nucleotide polymorphism genotyping. All variation in gene content was attributable to acquisition or loss of prophages, a molecular process that generated unique combinations of proven or putative virulence genes. Distinct serotype M3 genotypes experienced rapid population expansion and caused infections that differed significantly in character and severity. Molecular genetic analysis, combined with immunologic studies, implicated a 4-aa duplication in the extreme N terminus of M protein as a factor contributing to an epidemic wave of serotype M3 invasive infections. This finding has implications for GAS vaccine research. Genome-wide analysis of population-based strain samples cultured from clinically well defined patients is crucial for understanding the molecular events underlying bacterial epidemics. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
26. Intrahost Sequence Variation in the Streptococcal Inhibitor of Complement Gene in Patients with Human Pharyngitis.
- Author
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Matsumoto, Masakado, Hoe, Nancy P., Liu, Mengyao, Beres, Stephen B., Sylva, Gail L., Brandt, Claudia M., Haase, Gerhard, and Musser, James M.
- Subjects
STREPTOCOCCUS ,EPIDEMICS - Abstract
Selection of new variants of the streptococcal inhibitor of complement protein has been implicated in the perpetuation of epidemics caused by serotype M1 strains of group A Streptococcus (GAS). The frequency at which new streptococcal inhibitor of complement (Sic) variants arise in an infected individual is not known. To study this issue, the sic gene was sequenced in 100 isolates cultured from throat swabs of each of 20 patients with acute pharyngitis caused by serotype M1 GAS. Five patients were infected with GAS populations expressing 2 Sic variants characterized by deletion of a region of the protein. In contrast, no intrahost variation was detected in the number of a pentanucleotide repeat (CAAAA) that controls production of a bacterial cell-surface collagen-like protein by slipped-strand mispairing. Sic variation occurs at a sufficient frequency in vivo to result in mixed infections on the mucosal surface of human hosts, potentially contributing to pathogen survival. [ABSTRACT FROM AUTHOR]
- Published
- 2003
- Full Text
- View/download PDF
27. Genome sequence of a serotype M3 strain of group A Streptococcus: Phage-encoded toxins, the high-virulence phenotype, and clone emergence.
- Author
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Beres, Stephen B., Sylva, Gail L., Barbian, Kent D., Benfang Lei, Hoff, Jessica S., Mammarella, Nicole D., Meng-Yao Liu, Smoot, James C., Porcella, Stephen F., Parkins, Larye D., Campbell, David S., Smith, Todd M., McCormick, John K., Leung, Donald Y.M., Schlievert, Patrick M., and Musser, James M.
- Subjects
STREPTOCOCCUS ,GENE expression - Abstract
Examines the genome sequence of a serotype M3 strains of group A Streptococcus. Use of the genome of strain MGAS315; Composition of the genome; Cause of the variation in gene content.
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- 2002
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28. Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks.
- Author
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Smoot, James C., Barbain, Kent D., Van Gompel, Jamie J., Smoot, Laura M., Chaussee, Michael S., Sylva, Gail L., Sturdevant, Daniel E., Ricklefts, Stacy M., Porcella, Stephen F., Parkins, Larye D., Beres, Stephen B., Campbell, David S., Smith, Tood M., Qing Zhang, Kapur, Vivek, Daly, Judy A., Veasy, L. George, and Musser, James M.
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NECROTIZING fasciitis ,RHEUMATIC fever - Abstract
Assesses the genetic and molecular pathogenesis of group A Streptococcus (GAS) infection. Association between acute rheumatic fever and rheumatic heart disease; Genetic elements responsible for the diversity in the species; Involvement of additional secreted proteins in human–interactions.
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- 2002
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29. Genomic Analysis of emm59 Group A Streptococcus Invasive Strains, United States.
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Fittipaldi, Nahuel, Olsen, Randall J., Beres, Stephen B., Van Beneden, Chris, and Musser, James M.
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STREPTOCOCCUS ,INFECTION ,GENETICS ,EPIDEMICS - Abstract
The article discusses a study that identified a monoclonal cluster of invasive type emm59 group A streptococcus (GAS) infections in the San Francisco Bay area in California. The genomes of all available invasive emm59 GAS strains collected from 2000 to 2009 by the Active Bacterial Core surveillance arm of the U.S. Centers for Disease Control and Prevention were sequenced. It was shown that the level of genetic diversity seen among emm59 GAS strains in the U.S. was greater than that among strains from an epidemic in Canada. The authors also call for more intense monitoring for a potential shift in the epidemic behavior of emm59 GAS.
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- 2012
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30. Postpartum Group A Streptococcus Case Series: Reach Out to Infection Prevention!
- Author
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Alexander, Andrew J, Myers, Carol, Beres, Stephen B, Olsen, Randall J, Musser, James M, and Mangino, Julie E
- Abstract
A series of postpartum Streptococcus pyogenes infections prompted an investigation to rule out potential transmission by a health care worker. None of the hospital staff screened were colonized. All isolates were determined to be unrelated by molecular methods, including whole-genome sequencing. Thus, nosocomial transmission was considered unlikely. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
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