Search

Purpose: For the repair of atrial/ventricular septal defects (ASD/VSD), transthoracic echocardiography (TTE) is easy to perform, cost‐effective, and does not require general anesthesia and intubation. Still, TTE is not universally accepted. To compare efficacy and safety of TTE versus transesophageal echocardiography (TEE) to guide percutaneous ASD/VSD closure. Methods: PubMed, Embase, and the Cochrane library were searched for articles published from their inception to December 2023. The primary outcome was the procedural success rate. The secondary outcomes were residual shunt, late complications, procedure time, and fluoroscopy time. All meta‐analyses were performed using a random‐effects model. Results: Eight studies and 1295 patients were included. There were no significant differences between TTE and TEE regarding the procedural success rate (OR = 1.93, 95% CI: 0.90–4.13, p = 0.092; I2 = 52.2%, Pheterogeneity = 0.063) and residual shunt rate (OR = 0.81, 95% CI: 0.38–1.76, p = 0.600; I2 = 0%, Pheterogeneity = 0.518). Compared with TEE, TTE reduced the frequency of late complications (OR = 0.25, 95% CI: 0.14–0.43, p < 0.001; I2 = 13.8%, Pheterogeneity = 0.326), reduced the procedure time (WMD = −8.92, 95% CI: −12.08, −5.75, p < 0.001; I2 = 87.4%, Pheterogeneity < 0.001), and reduced the fluoroscopy time (WMD = −5.08, 95% CI: −9.59, −0.56, p = 0.028; I2 = 95.6%, Pheterogeneity < 0.001). The sensitivity analyses showed that the results of the meta‐analyses were robust. Conclusion: Compared with TEE, TTE showed no differences regarding the rates of success and residual shunt, but there were lower rates of late complications and shorter procedure and fluoroscopy times. [ABSTRACT FROM AUTHOR]

Purpose: Hepatocellular carcinoma has become one of the severe diseases threatening human health. T cell exhaustion is deemed as a reason for immunotherapy resistance. However, little is known about the roles of CD8 Tex-related lncRNAs in HCC. Materials and Methods: We processed single-cell RNA sequencing to identify CD8 Tex-related genes. CD8 Tex-related lncRNAs were identified based on their correlations with mRNAs. Unsupervised clustering approach was used to identify molecular clusters of CD8 Tex-related lncRNAs. Differences in prognosis and immune infiltration between the clusters were explored. Machine learning algorithms were used to construct a prognostic signature. Samples were classified as low- and high-risk groups based on their risk scores. We identified prognosis-related lncRNAs and constructed a ceRNA network. In vitro experiments were conducted to investigate the impacts of CD8 Tex-related lncRNAs on proliferation and apoptosis of HCC cells. Results: We clarified cell types within two HCC single-cell datasets. We identified specific markers of CD8 Tex cells and analyzed their potential functions. Twenty-eight lncRNAs were identified as CD8 Tex-related. Based on CD8 Tex-related lncRNAs, samples were categorized into two distinct clusters, which exhibited significant differences in survival rates and immune infiltration. Ninety-six algorithm combinations were employed to establish a prognostic signature. RSF emerged as the one with the highest C-index. Patients in high- and low-risk groups exhibited marked differences in prognosis, enriched pathways, mutations and drug sensitivities. MCM3AP-AS1, MAPKAPK5-AS1 and PART1 were regarded as prognosis-related lncRNAs. A ceRNA network was constructed based on CD8 Tex-related lncRNAs and mRNAs. Experiments on cell lines and organoids indicated that downregulation of MCM3AP-AS1, MAPKAPK5-AS1 and PART1 suppressed cell proliferation and induced apoptosis. Conclusion: CD8 Tex-related lncRNAs played crucial roles in HCC progression. Our findings provided new insights into the regulatory mechanisms of CD8 Tex-related lncRNAs in HCC. [ABSTRACT FROM AUTHOR]

Unknown actuator failures are inevitable in practical systems. At the same time, time delay exists in many physical actuators, and the system performance will be affected by such actuator delay and faults. However, the results of studies that attempt to compensate for unknown failures of actuators with time delay are still very limited. In this paper, such a problem is studied, and an adaptive control scheme is proposed based on backstepping approaches. First, the input delay of actuator faults and output disturbances are transformed into unknown effects on the output signal. In the backstepping recursive design, these unknown effects will accumulate to the last step of the controller design. Then, a new Lyapunov function is constructed by introducing auxiliary signals to prove the stability of the system. It is shown that the proposed control scheme can compensate for the effects caused by unknown actuator failures and input delays. The stability of the closed-loop system can be guaranteed by this adaptive controller. Finally, simulation studies are used to verify the effectiveness of the proposed scheme. [ABSTRACT FROM AUTHOR]

The precise calculation and effective control of the horizontal displacement of deep foundation pit retaining structures are critical for foundation pit support design and construction. Based on stress–strain linear elastomer theory and considering the deformation coordination between an enclosure wall and its internal support member, a formula for the redundant restraint force acting on the retaining wall was derived through the unit load method and the principle of elastic superposition. Moreover, a method for calculating the horizontal displacement of the retaining structure of a deep foundation pit was formulated, which is convenient for engineering applications. The method can also be used to calculate the horizontal displacement of cantilevered and anchored retaining structures when the loading conditions of the deep foundation pit and the relevant parameters of the enclosure structure are known. A case study was conducted on a standard section with an excavation width H of 19.3 m and an excavation depth h of 17.8 m. The structural parameters of the enclosure wall, along with the elastic support stiffness coefficient and soil layer parameters of the pit, were inputted into a MATLAB calculation code. Then, four internal support constraint forces Fi and the calculated values for the horizontal displacement of the enclosure wall were obtained after running the code. The calculated curve closely matched the curve of values measured in the field. The horizontal displacements of the top of the wall of several cement–soil gravity enclosure structures mentioned in the literature were also calculated. The results of these calculations were then compared with the measured data and corresponding data from the literature. The examples provided clear evidence demonstrating that the proposed method is highly reliable for calculating the horizontal displacement of deep foundation pit enclosure structures. [ABSTRACT FROM AUTHOR]

It is well known that unknown modeling errors cannot be avoided in practice. Such unmeasured uncertainties are usually denoted as unknown nonlinear functions that exist in every channel of the system equation. This paper aims to develop an output feedback adaptive control scheme by constructing state estimation filters to address such unknown modeling errors. In the controller design, these uncertainties caused by modeling errors will be accumulated to the last step for compensation. Unknown parameters existing in the upper bound functions of unknown nonlinear functions and system parameters are estimated synchronously based on tuning function approaches. It is shown that the designed output feedback controller can ensure the stability and tracking performance of the closed‐loop system, and the transient performance in terms of a truncated norm is also established. [ABSTRACT FROM AUTHOR]

The Eimeria tenella Yulin strain (EtYL), which is sensitive to most anti-coccidial drugs, was isolated in the Yulin area of Guangxi, China. Then, Eimeria tenella Yulin precocious line (pEtYL), a precocious line with a prepatent period of 108 h, was obtained through early selection. The biological characteristics of pEtYL, including its morphology, purity, oocyst excretion curve, reproductive capacity, pathogenicity, immunogenicity, and preservation time, were comprehensively analyzed. The results showed that the isolated precocious line of E. tenella exhibited high purity, relatively weak pathogenicity, and good immunogenicity and can be used as a live vaccine line for chicken coccidiosis. [ABSTRACT FROM AUTHOR]

Long noncoding RNAs (lncRNAs) are regulatory transcripts during protozoan infections in the host intestinal epithelial cells (IECs). Apicomplexan Eimeria falciformis sporozoite extracellular vesicles (EVs) contain virulence factors that modulate host IECs pro‐inflammatory genes and immune responses. In this study, E. falciformis sporozoites were made to interact with inactivated host cells, and the parasite EVs were separated from total secretome by ultracentrifugation and purified on density gradient medium. Dose‐dependent bio‐activity of E. falciformis EVs was investigated by RNA sequencing, functional annotation and quantitative PCR. It was found that E. falciformis EVs induced mRNA, circRNA, and lncRNA expressions in mouse IECs. Of 38, 217 lncRNAs assembled, 157 and 152 were upwardly and downwardly expressed respectively. Differentially expressed lncRNAs were associated with cytokines, pyroptosis, and immune signaling pathways including FoxO, NF‐κB, MAPK, and TGF‐β. In essence, E. falciformis EVs altered host cell RNA expressions during the interaction with host IECs. Also, differentially expressed lncRNAs are potential diagnostic transcripts during Eimeria infections. [ABSTRACT FROM AUTHOR]

The new generation of communication systems requires radio frequency (RF) filters with better performance indicators, and traditional RF filters can no longer satisfy the requirements of increasingly sophisticated wireless communication equipment. Piezoelectric Film bulk acoustic resonators (FBARs) have gradually become a focus of communication system research. In this study, the temperature effect was considered in the FBAR electrical model. SiO2 with a positive temperature coefficient was placed under the bottom electrode to perform temperature compensation. COMSOL software was used to study the shape of the electrode of the FBAR unit, the irregular shape of the electrode could obtain a smoother resonant frequency curve, and the common cavity and back erosion structure of the FBAR unit were studied, to extract the corresponding dielectric loss and mechanical loss of the piezoelectric layer, and to optimize the one-dimensional electrical model further. The optimized electrical model was used to design an FBAR filter. The center frequency was 3.52 GHz, the bandwidth was 115 MHz, the insertion loss was 0.87 dB, the in-band ripple was 1.32 dB, the out-of-band rejection was better than − 40 dB, and the absolute value of temperature coefficient of frequency was 7.09 ppm/°C, basically achieving the expected performance, which can be applied to the design of RF filters in mobile phones and other wireless terminals where the temperature requirement is harsh, and provides a solution for frequency selection and control in the field of high frequency communication. [ABSTRACT FROM AUTHOR]

In this paper, the event-triggered control problem is investigated using backstepping techniques for nonlinear systems with dead-zone input. The external disturbance and unknown parameters are also considered in the controller's design. It is well known that errors in input signal measurements are inevitable. In event-triggered control, such errors will directly affect whether the control signal is updated. This measurement error can be seen in the form of interference to the threshold. Therefore, unlike traditional event-triggered control, the existence of threshold disturbance is considered in the controller's design. The proposed controller can not only compensate for the uncertainties caused by external disturbance and unknown parameters but can also suppress the unknown effects caused by threshold interference. In addition, to obtain a continuous controller, a smooth function is constructed to approximate the discontinuous sign function. In this way, Zeno behavior is successfully avoided. The boundedness of all signals and the tracking performance of the system can be guaranteed by the proposed control scheme. Numerical simulation and actual system simulation demonstrate the effectiveness of the proposed control scheme. The comparative simulation results also verify this event-triggered controller's advantages, including better tracking performance and fewer trigger times. [ABSTRACT FROM AUTHOR]

Human aging is invariably accompanied by a decline in renal function, a process potentially exacerbated by uremic toxins originating from gut microbes. Based on a registered household Chinese Guangxi longevity cohort (n = 151), we conducted comprehensive profiling of the gut microbiota and serum metabolome of individuals from 22 to 111 years of age and validated the findings in two independent East Asian aging cohorts (Japan aging cohort n = 330, Yunnan aging cohort n = 80), identifying unique age‐dependent differences in the microbiota and serum metabolome. We discovered that the influence of the gut microbiota on serum metabolites intensifies with advancing age. Furthermore, mediation analyses unveiled putative causal relationships between the gut microbiota (Escherichia coli, Odoribacter splanchnicus, and Desulfovibrio piger) and serum metabolite markers related to impaired renal function (p‐cresol, N‐phenylacetylglutamine, 2‐oxindole, and 4‐aminohippuric acid) and aging. The fecal microbiota transplantation experiment demonstrated that the feces of elderly individuals could influence markers related to impaired renal function in the serum. Our findings reveal novel links between age‐dependent alterations in the gut microbiota and serum metabolite markers of impaired renal function, providing novel insights into the effects of microbiota‐metabolite interplay on renal function and healthy aging. [ABSTRACT FROM AUTHOR]

An adequately calibrated hydraulic model is critically to the water distribution digital twin, which requires accurate representation of a water distribution network (WDN) in near real-time. It is thus imperative to construct an extended-period simulation model that captures the network's baseline normal and abnormal diurnal demand patterns, which are usually derived using monitored flow data. However, from a practical field aspect, it remains challenging to calibrate multiple demand groups of varying diurnal patterns in large-scale WDNs, as flow data are typically collected sparsely due to cost considerations, while pressure sensors are commonly deployed throughout the network. In this paper, we propose a new pressure-based demand aggregation and pattern calibration method that leverages on monitoring pressure data to aggregate demands, identifying abnormal consumptions, and calibrating the diurnal patterns of various demand groups. The new method is integrated with previously developed model calibration framework and applied to a large-scale WDN system having more than 330 km of underground water pipelines with weekly averaged pressure and flow data, as derived from a maximum historical period of nine months. Key findings from our case study analysis for the seven averaged days (Monday to Sunday) include: (1) calibrating the system's flow balance to within 99% average accuracy by identifying and calibrating five unique demand patterns, inclusive of those associated with abnormal consumptions, via grouping 34 available pressure sensors; (2) calibrating the system's energy balance to within 95% average accuracy by iterating the simulated pressures against representative monitored pressure profiles of the different demand groups during the flow calibration process; and (3) achieving an average 2.5% accuracy improvement for the overall energy calibration, relative to that of the previous calibration approach. Throughout the solution process, significant engineering judgment is adopted, coupled with optimization analyses, to calibrate the system's flow and energy balances while meeting the model constraints and data availability. [ABSTRACT FROM AUTHOR]

Background: Extracellular vesicles (EVs) are membranous structures that are formed during pathophysiology, host-parasite interactions and parasite motility. Typically, apicomplexan-infected host cells secrete EVs which traverse local and systemic strata of the host as the parasites develop. Methods: Extracellular vesicles were isolated from the caecum and serum of Eimeria falciformis-infected mice during oocyst ingestion (0 h post-infection [0 hpi]), merozont stages 1 and 2 (68 and 116 hpi), oocyst shedding (7 days post-infection [7 dpi]) and host recovery (10 dpi) and subsequently characterized and profiled by tandem mass tag (TMT). Results: With the progression of E. falciformis life stages, subpopulation of EVs bearing EV biomarkers, including CD9, CD82, heat shock protein 70 (HSP70) and major histocompatibility complex (MHC) molecules, increased. A total of 860 and 1024 differentially expressed proteins were identified in serum EVs (sEVs) and caecum EVs (cEVs), respectively. Identified immune-related molecules (such as cytokines, receptors, immunoglobins, complements, hormones, inflammasomes), ion exchange and cell death-associated proteins were significantly expressed, at least during the E. falciformis first and second merozont stages. Bioinformatics assessment indicated that sEV proteins were at all time points implicated in antigen processing and presentation as well as natural killer cell-mediated cytotoxicity (68 hpi), complement activation/blood coagulation (116 hpi/10 dpi) and catabolic activities (7 dpi). In contrast, cEV proteins were involved in catabolic process, ion transport and antigen presentation (68 and 116 hpi). Host response to E. falciformis infection was similar to intestinal bacterium at 7 dpi and cell adhesion and intercellular protein transport at 10 dpi. In both systems, ferroptosis and necroptosis were common across the parasite's infectious cycle while apoptosis occurred at 68 hpi. Conclusion: The proteomic data indicate that E. falciformis infection co-opts cellular and humoral responses through EV secretions, and that, host cell death and ionic imbalance are associated with E. falciformis infection. This study offers additional insight into host-parasite interactions and host regulatory EV proteins as potential disease indicators or diagnostic molecules. [ABSTRACT FROM AUTHOR]

In practical operation, the system parameters of a permanent magnet linear motor are affected by unknown factors, including nonlinear friction, sudden load changes, thrust fluctuations, and so on. Therefore, a controller designed for fixed parameters often cannot produce satisfactory results. To solve this problem, a novel design method for permanent magnet linear motor systems based on backstepping is proposed in this article. The proposed control scheme does not require the values or range of changes of the system parameters in advance but constructs an update law to perform online parameter estimation. A difficulty encountered in controller design and stability analysis is the estimation of the unknown coefficient of a single-variable state, which can be considered as a virtual input. In this article, the estimated virtual control coefficient is introduced into the coordinate transformation to construct a novel coordinate transformation. However, introducing the estimated coefficient into the virtual control makes the derivative of the Lyapunov function used in the stability analysis more complex. This novel nonlinear dynamic term can be precisely cancelled by changing the update law of the coefficient. Finally, simulations are performed to assess the performance of the proposed control scheme against that of a traditional proportional–integral–derivative controller, and the simulation results show that the proposed control law can effectively improve the system performance. [ABSTRACT FROM AUTHOR]

The synchronization of master-slave hyperchaotic systems is investigated by intermittent control and proved by the Lyapunov stable theory. Meanwhile, a new secure communication scheme is designed for the continuous and digital information. The encrypted information is transmitted to receiver through the intermittent controller, which reduces the disturbance to the synchronization of master-slave systems and improves the security and reliability of secure communication. Before transmitting to the receiver, the continuous signal is firstly modulated and masked by the chaotic signals. Furthermore, an encryption algorithm for the digital information of color image is proposed by the pseudo-random sequences of Chen hyperchaotic system, and then the encrypted image is modulated and masked by the variables of the master system. The original image can be decrypted successfully at the receiving end after the slave system is synchronized with the master system. Finally, the feasibility and effectiveness of this scheme are verified by simulation experiments. In addition, the security analysis of the image encryption algorithm is also discussed, such as key sensitivity, correlation coefficient, NIST test, and return map. [ABSTRACT FROM AUTHOR]

An intermittent controller for robotic manipulator in task space was developed in this paper. In task space, for given a desired time-varying trajectory, the robot end-effector can track the desired target under the designed intermittent controller. Different from most of the existing works on control of robotic manipulator, the intermittent control for robotic manipulator is discussed in task space instead of joint space. Besides, the desired trajectory can be time-varying and not limited to constant. As a direct application, the authors implemented the proposed controller on tracking of a two-link robotic manipulator in task space. Numerical simulations demonstrate the effectiveness and feasibility of the proposed intermittent control strategy. [ABSTRACT FROM AUTHOR]

Practical synchronization between two n -dimensional nonautonomous chaotic systems is achieved by the method of event-triggered control in the case of parameter mismatch. This study focuses on parameter mismatch and its effect on synchronization performance. The synchronization criterion is deduced in the form of linear matrix inequality. The synchronization error bound is analytically estimated, which can reveal the relationships between the synchronization error and the parameters. It is proven that there exists a lower bound for the inter-event times between two event-triggered moments, which means no Zeno behavior will occur in this control scheme. The obtained results are applied to a gyrostat system. Subsequently, numerical simulations demonstrate the effectiveness of the control strategy. [ABSTRACT FROM AUTHOR]

Purpose. Mesenchymal stem cells (MSCs) and their derivant are among the promising treatments for intrauterine adhesion (IUA); they have been reported to repair the endometrial injury by proliferating endometrial cells. However, the signal pathways involved are not clear. This study investigated the role of human umbilical cord mesenchymal stem cell-derived conditioned medium (hUCMSC-CM) in relieving IUA to find out whether Wnt/β-catenin signaling was involved, and if so, to determine the possible ligands. Methods. After endometrial epithelial cells (EECs) were treated with hUCMSC-CM, their proliferation and migration were measured by the CCK8 assay and the scratch assay. The activation of Wnt/β-catenin signaling was measured by Western blots, fluorescent staining, and T-cell factor/lymphoid enhancer factor (TCF/LEF) luciferase. A Wnt inhibitor (XAV393) was used to inhibit the proliferation effect of hUCMSC-CM in EECs. Wnt5a expression in hUCMSC was measured by Western blots and fluorescent staining, and Wnt5a in hUCMSC-CM was detected by enzyme-linked immunosorbent assay (ELISA), to further clarify the mechanism. Results. As shown by the CCK8 assay, hUCMSC-CM promoted proliferation and migration of EECs. The expression of β-catenin, c-myc, and cyclin D1 increased in EECs after being treated with hUCMSC-CM. Moreover, hUCMSC-CM was found to promote β-catenin delivery into nuclei by Western blot and fluorescent staining; meanwhile, the inhibitor (XAV393) could restrain this process and inhibit the effect of hUCMSC-CM on EEC proliferation. Wnt5a was detected in hUCMSCs and hUCMSC-CM, which might be a potential therapeutic target. Conclusion. This study demonstrated that hUCMSC-CM promoted human endometrial cell proliferation through Wnt/β-catenin signaling, and Wnt5a might be a potential activator. This would be one of the activating signal pathways in the MSC-related treatment of IUA. [ABSTRACT FROM AUTHOR]

Background: Protozoan parasite secretions can be triggered by various modified media and diverse physicochemical stressors. Equally, host-parasite interactions are known to co-opt the exchange and secretion of soluble biochemical components. Analysis of Eimeria falciformis sporozoite secretions in response to interaction with mouse intestinal epithelial cells (MIECs) may reveal parasite secretory motifs, protein composition and inflammatory activities of E. falciformis extracellular vesicles (EVs). Methods: Eimeria falciformis sporozoites were allowed to interact with inactivated MIECs. Parasite secretions were separated into EV and vesicle-free (VF) fractions by discontinuous centrifugation and ultracentrifugation. Secreted EVs were purified in an iodixanol density gradient medium and the protein composition of both EV and VF fractions were analyzed by liquid chromatoraphy-tandem mass spectroscopy. The inflammatory activities of E. falciformis sporozoite EV on MIECs were then investigated. Results: During the interaction of E. falciformis sporozoites with inactivated MIECs, the parasite secreted VF and vesicle-bound molecules. Eimeria falciformis vesicles are typical pathogenic protozoan EVs with a mean diameter of 264 ± 2 nm, and enclosed heat shock protein (Hsp) 70 as classical EV marker. Refractile body-associated aspartyl proteinase (or eimepsin), GAP45 and aminopeptidase were the main components of E. falciformis sporozoite EVs, while VF proteins include Hsp90, actin, Vps54 and kinases, among others. Proteomic data revealed that E. falciformis EV and VF proteins are aggregates of bioactive, antigenic and immunogenic molecules which act in concert for E. falciformis sporozoite motility, pathogenesis and survival. Moreover, in MIECs, E. falciformis EVs induced upregulation of gene expression and secretion of IL-1β, IL-6, IL-17, IL-18, MCP1 as well as pyroptosis-dependent caspase 11 and NLRP6 inflammasomes with the concomitant secretion of lactate dehydrogenase. Conclusions: Eimeria falciformis sporozoite interaction with MIECs triggered the secretion of immunogenic and antigenic proteins. In addition, E. falciformis sporozoite EVs constitute parasite-associated molecular pattern that induced inflammatory response and cell death. This study offers additional insight in the secretion and protein composition of E. falciformis secretomes as well as the proinflammatory functions of E. falciformis sporozoite EVs. [ABSTRACT FROM AUTHOR]

To improve the efficiency of Smart Water Grid (SWG) operation, it is of great importance to timely detect and classify anomaly events. Authors have developed a comprehensive solution framework for anomaly detection. However, the framework may detect undesirable false alarms, which will incur wasteful field investigation. Therefore, it is imperative to reduce the number of false alarms for real world SWG operation. In this paper, the anomaly event classification has been improved by an algorithm of trend change detection in two phases including (1) detecting trend change in single sensor, and (2) identifying the effective evaluation time window by correlating the flow anomaly trend period and pressure anomaly trend periods to filter the detected events. A real case study has been conducted to demonstrate the application of the proposed algorithm; the number of false alarms is reduced by as much as 31% without compromising the detection accuracy. [ABSTRACT FROM AUTHOR]

Due to the increasing deployment of sensors in water distribution networks (WDNs) for continuous monitoring, hydrodynamic data are readily available for engineers to improve the daily operations of WDNs. In collaboration with Public Utility Board (PUB), Singapore's National Water Agency, an alternative model calibration approach using continuously monitored data is proposed to facilitate PUB's smart water grid (SWG). The generic approach was developed as an integrated solution methodology for practical engineers to conduct a series of systematic analyses daily, namely, (1) estimating the system's daily nonrevenue water (NRW) volume and NRW time series; (2) adjusting the available pumps' operational curves and control statuses, followed by calibrating the system's net demand pattern to fulfill the flow balance accounting for the actual consumption; (3) identifying and rectifying possible offsets in the monitored pressure head values for each sensor station; (4) performing model calibration with anomaly localization analysis when the system's total NRW volume exceeds its assumed background NRW volume; and (5) calibrating other physical properties to fulfill the system's energy balance, especially for the peak demand hours. The effectiveness of our proposed approach was then tested and verified using a real-world WDN having total pipe length of >650 km with available monitoring data. Key findings from the case study analysis include (1) anomaly events localized including, but not limited to, five out of six reported leaks for the selected week to within 400 m with lead time of 1–2 days; (2) the system's initial flow imbalance addressed by estimating the daily NRW volume and localizing the possible anomaly events; and (3) pipe roughness values calibrated to further improve the energy balance in the system, especially during the peak demand hours, by attaining an average mean absolute percentage error (MAPE) score of 2.5%. [ABSTRACT FROM AUTHOR]

By using a controller of uniformly bounded sine function, the problem of chaos anti-control for continuous linear systems is studied, and the dynamic characteristics of the controlled system are analyzed via the Lyapunov exponent spectrum and bifurcation diagram. The controlled system can be at a state of periodic motion, chaos or hyperchaos with multiple positive Lyapunov exponents when the parameters of controller belong to different intervals. Based on the hyperchaotic system, a new scheme of chaotic image encryption is proposed and it is given in the following aspects: (1) five chaotic sequences are generated from the hyperchaotic system, and the preprocessed pseudo-random sequences are used in the scrambling of the pixel positions; (2) the pixel values of image are encrypted by the combination of multiple pseudo-random sequences; (3) though the double chaotic encryption, the security of the chaotic stream cipher is analyzed by means of key sensitivity analysis, histogram analysis and information entropy analysis, etc. Finally, the experimental results show the scheme is effective and feasible in image encryption, and it can resist some attacks, such as the differential attacks, chosen-plain-text attacks, and clipping attacks. [ABSTRACT FROM AUTHOR]

Background: Bladder cancer (BLCA) is a malignant tumor with a complex molecular mechanism and high recurrence rate in the urinary system. Studies have shown that pyroptosis regulates tumor cell proliferation and metastasis and affects the prognosis of cancer patients. However, the role of pyroptosis-related (PR) genes or long non-coding RNAs (lncRNAs) in BLCA development is not fully understood. Methods: We comprehensively analyzed the molecular biological characteristics of PR genes in BLCA, including copy number variation, mutations, expression and prognostic value based on TCGA database. We then identified PR lncRNAs with prognostic value based on the expression of PR genes and performed a consistent clustering analysis of 407 BLCA patients according to the expression of prognosis-related PR lncRNAs and identified two clusters. The least absolute shrinkage and selection operator (LASSO) regression was used to establish a PR lncRNA signature and calculate the risk score associated with the prognosis of patients with BLCA. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) were used to evaluate the possible functions of PR lncRNA signature. We also evaluated the relationship between the risk score and tumor immune microenvironment (TIME). Results: A total of 33 PR genes were obtained in our study and 194 prognosis-related PR lncRNAs were identified. We also constructed a signature consisting of eight-PR-lncRNAs and divided patients into high- and low-risk groups. The overall survival rate of patients with a high risk was significantly lower than patients with a low risk. The risk score was significantly correlated with the degree of infiltration of multiple immune cell subtypes and positively correlated with multiple immune checkpoint genes expression in BLCA. Enrichment analyses showed that these lncRNAs are involved in human immune regulatory functions and immune-related pathways. Conclusion: Our study comprehensively studied the molecular biological characteristics of PR genes BLCA, and the eight-PR-lncRNA signature we identified might play a crucial role in tumor immunity and may be able to predict the prognosis of BLCA patients, providing a theoretical basis for an in-depth study of the relationship between the prognosis and TIME. [ABSTRACT FROM AUTHOR]

The dynamical characteristics of a gyrostat system with intermittent forcing are investigated, the main work and contributions are given as follows: (1) The gyrostat system with an intermittent forcing is studied, and its dynamical characteristics are investigated by the corresponding Lyapunov exponent spectrums and bifurcation diagrams with respect to the amplitude of intermittent forcing. The modified gyrostat system exists chaotic motion when the amplitude of intermittent forcing belongs to a certain interval, and it can be at a state of stable point or periodic motion by the design of amplitude. (2) The gyrostat system with multiple intermittent forcings is also investigated through the combination of Lyapunov exponent spectrums and bifurcation diagrams, and it behaves periodic motion or chaotic motion when the amplitude or forcing width is different. (3) By the selection of parameters in intermittent forcings, the modified gyrostat system is at a state of stable point, periodic motion or chaotic motion. Numerical simulations verify the feasibility and effectiveness of the modified gyrostat system. [ABSTRACT FROM AUTHOR]

Systemic lupus erythematosus (SLE) is a heterogeneous disease characterized by the production of abnormal autoantibodies and immune complexes that can affect the organ and organ systems, particularly the kidneys and the cardiovascular system. Emerging evidence suggests that dysregulated lipid metabolism, especially in key effector cells, such as T cells, B cells, and innate immune cells, exerts complex effects on the pathogenesis and progression of SLE. Beyond their important roles as membrane components and energy storage, different lipids can also modulate different cellular processes, such as proliferation, differentiation, and survival. In this review, we summarize altered lipid metabolism and the associated mechanisms involved in the pathogenesis and progression of SLE. Furthermore, we discuss the recent progress in the role of lipid metabolism as a potential therapeutic target in SLE. [ABSTRACT FROM AUTHOR]

Background: DNA polymerase β is one of the key enzymes involved in DNA damage repair and its proper expression is strictly controlled within different cells. We previously reported that three genetic mutations in the promoter region of the polb gene are prevalent in the Chinese Han population and two types of mutation are associated with thymic hyperplasia. The purpose of this study was to explore whether other mutated sites exist within the promoter region of the polb gene. Methods: Genomic DNAs of 421 healthy Chinese Han individuals were extracted from whole blood samples and used for gene amplification of the promoter region of the polb gene. After gel purification, PCR amplicons were sequenced by the Sanger sequencing method and used for sequence alignment with the Lasergene program. PCR products with novel mutations were then subcloned into luciferase reporter plasmid pGL4.10 and transfected into 293T cells for dual‐luciferase activity analysis. Results: In total, 11 mutated sites were detected in the Chinese Han population and eight of these were reported for the first time. Using a dual luciferase reporter system, it was found that one novel mutation −142 C > G could decrease the transcription activity of the polb gene, whereas two novel mutations, −152_−151insC and −218 C > G, could significantly increase the transcription activity of the polb gene. Conclusions: High polymorphic sites could be found in the promoter region of polb gene and approximately half of them could influence its transcription activity. [ABSTRACT FROM AUTHOR]

The aim of this study was to 1) investigate the effects of 27 CYP3A4 variants on the metabolism of osimertinib and 2) study the interactions between osimertinib and others as well as the underlying mechanism. A recombinant human CYP3A4 enzymatic incubation system was developed and employed to determine the kinetic profile of CYP3A4 variants. Ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) was applied to detect the concentration of the main metabolite, AZ5104. The results demonstrated that the relative clearance rates of CYP3A4.19, 10, 18, 5, 16, 14, 11, 2, 13, 12, 7, 8, and 17 in catalyzing osimertinib were significantly reduced to a minimum of 25.68% compared to CYP3A4.1, while those of CYP3A4.29, 32, 33, 28, 15, 34, and 3 were obviously enhanced, ranging from 114.14% to 284.52%. The activities of the remaining variants were almost equal to those of CYP3A4.1. In addition, 114 drugs were screened to determine the potential interaction with osimertinib based on the rat liver microsome (RLM) reaction system. Sixteen of them inhibited the production of AZ5104 to 20% or less, especially proton pump inhibitors, among which the IC50 of rabeprazole was 6.49 ± 1.17 μM in RLM and 20.39 ± 2.32 μM in human liver microsome (HLM), with both following competitive and non-competitive mixed mechanism. In an in vivo study, Sprague–Dawley (SD) rats were randomly divided into groups, with six animals per group, receiving osimertinib with or without rabeprazole, omeprazole, and lansoprazole. We found that the AUC(0–t), AUC(0–∞), and Cmax of osimertinib decreased significantly after co-administration with rabeprazole orally, but they increased remarkably when osimertinib was administered through intraperitoneal injection. Taken together, our data demonstrate that the genetic polymorphism and proton pump inhibitors remarkably influence the disposition of osimertinib, thereby providing basic data for the precise application of osimertinib. [ABSTRACT FROM AUTHOR]

Porcine circovirus 2 (PCV2) has been recognized as an immunosuppressive pathogen. However, the crosstalk between this virus and its host cells in related signaling pathways remains poorly understood. In this study, the expression profiles of 84 genes involved in transforming growth factor-beta (TGF-β) signaling pathway were probed in PCV2b-infected primary porcine alveolar macrophages (PAMs) by using an RT2 profiler PCR array system. The protein expression levels of cytokines involved in the TGF-β signaling pathway were determined with a RayBiotech fluorescent Quantibody® porcine cytokine array system. Results showed that 48, 30, and 42 genes were differentially expressed at 1, 24, and 48 h after infection, respectively. A large number of genes analyzed by a co-expression network and implicated in transcriptional regulation and apoptosis were differentially expressed in PCV2b-infected PAMs. Among these genes, TGF-β, interleukin-10, CCAAT/enhancer-binding protein beta (C/EBPB), growth arrest, and DNA-damage-inducible 45 beta (GADD45B), and BCL2 were upregulated. By contrast, SMAD family member 1 (smad1) and smad3 were downregulated. These results suggested that the TGF-β signaling pathway was repressed in PAMs at the early onset of PCV2 infection. The inhibited apoptosis was indicated by the upregulated C/EBPB, GADD45B, and BCL2, and by the downregulated smad1 and smad3, which possibly increased the duration of PCV2 replication-permissive conditions and caused a persistent infection. Our study may provide insights into the underlying antiviral functional changes in the immune system of PCV2-infected pigs. [ABSTRACT FROM AUTHOR]

Apicomplexans are important pathogens that cause severe infections in humans and animals. The biology and pathogeneses of these parasites have shown that proteins are intrinsically modulated during developmental transitions, physiological processes and disease progression. Also, proteins are integral components of parasite structural elements and organelles. Among apicomplexan parasites, Eimeria species are an important disease aetiology for economically important animals wherein identification and characterisation of proteins have been long-winded. Nonetheless, this review seeks to give a comprehensive overview of constitutively expressed Eimeria proteins. These molecules are discussed across developmental stages, organelles and sub-cellular components vis-à-vis their biological functions. In addition, hindsight and suggestions are offered with intention to summarise the existing trend of eimerian protein characterisation and to provide a baseline for future studies. [ABSTRACT FROM AUTHOR]

RNAs are a class of molecules and the majority in eukaryotes are arbitrarily termed non- coding transcripts which are broadly classified as short and long non-coding RNAs. Recently, knowledge of the identification and functions of long non-coding RNAs have continued to accumulate and they are being recognized as important molecules that regulate parasite-host interface, parasite differentiation, host responses, and disease progression. Herein, we present and integrate the functions of host and parasite long non-coding RNAs during infections within the context of epigenetic re-programming and molecular crosstalk in the course of host-parasite interactions. Also, the modular range of parasite and host long non-coding RNAs in coordinated parasite developmental changes and host immune dynamic landscapes are discussed. We equally canvass the prospects of long non-coding RNAs in disease diagnosis and prognosis. Hindsight and suggestions are offered with the aim that it will bolster our understanding for future works on host and parasite long non-coding RNAs. [ABSTRACT FROM AUTHOR]

Necrotic enteritis (NE), caused by Clostridium perfringens , is an economically important disease in the broiler. Among normal flora in the broiler intestinal region, Clostridium butyricum has been identified as a probiotic agent that reduces the susceptibility of broilers to C. perfringens. However, the effects of C. butyricum supplement on broiler intestinal integrity during NE are largely unknown. In this study, we investigated the effects of C. butyricum on the growth performance, intestinal morphology and barrier function, and the functions of immune-related cytokines under NE in broilers. Chickens were divided into five groups: control group (NC), supplement C. butyricum only group (CB), NE-infected group (PC), supplement C. butyricum from Day 14 (NECB1) to Day 22 NE-infected group, and supplement C. butyricum from Day 1 (NECB2) to Day 22 NE-infected group. The results showed that there were significantly decreased average daily weight gain and increased feed conversion rate in the infected group (PC) compared with the C. butyricum -supplemented groups (NECB1 and NECB2) through the diet. Histopathological observation on the Hematoxylin–Eosin staining avian small intestine sections revealed that supplementation of C. butyricum (NECB1 and NECB2) could increase the intestinal villus height/crypt depth and lessen the intestinal damage under NE. ELISA and Limulus test showed that broilers infected with NE (PC) had higher serum IgA and lipopolysaccharide content; however, after C. butyricum supplementation (NECB1 and NECB2), they returned to a normal level. Furthermore, real-time PCR and Western blot results indicated that compared with PC, supplementing C. butyricum (NECB1 and NECB2) could initialize the expressions of genes related to the intestinal barrier-associated molecules (such as CLDN-1, CLDN-3, OCLN, MUC2, ZO-1, and CLDN5), cytokines (such as IL-10, IL-6, and TGFB1), and C. perfringens plc gene expression. Moreover, the results detected by the Ussing chamber suggested that C. butyricum (NECB1 and NECB2) could amend the decrease in conductivity value and short-circuit current value caused by NE. In addition, NECB2 significantly reduced the upregulation of fluorescein isothiocyanate–dextran flux caused by the NE disease. In conclusion, these findings suggest that dietary supplementation of C. butyricum in broilers with NE improved chicken growth performance, intestinal integrity and barrier function, and immunological status. Notably, no statistical difference was observed with the addition of C. butyricum on day 1 or day 14. [ABSTRACT FROM AUTHOR]

Knee osteoarthritis (KOA) is a major cause of disability in elderly individuals. Dicoumarol is a coumarin-like compound derived from sweet clover [Melilotus officinalis (L.) Pall]. It has been suggested that dicoumarol exhibits various types of pharmacological activities, including anticoagulant, antitumor and antibacterial effects. Due to its various biological activities, dicoumarol has a potential protective effect against OA. Therefore, the present study aimed to assess the effects of dicoumarol on knee osteoarthritis. In the present study, dicoumarol was found to protect rat synoviocytes from lipopolysaccharide (LPS)-induced cell apoptosis. Western blot analysis showed that dicoumarol significantly reduced the protein expression levels of fibrosis-related markers and inflammatory cytokines (Tgfb, Timp, Col1a, Il1b and Il18). The inhibitory rates of these proteins were all >50% (P<0.01) compared with those in the LPS and ATP-induced group. Consistently, the mRNA expression levels of these markers and cytokines were decreased to normal levels by dicoumarol after the treatment of rat synovial fibroblasts with LPS and ATP. Mechanistic studies demonstrated that dicoumarol did not affect NF-κB signaling, but it did directly interact with NOD-like receptor protein 3 (NLRP3) to promote its protein degradation, which could be reversed by MG132, but not NH4Cl. The protein half-life of NLRP3 was accelerated from 26.1 to 4.3 h by dicoumarol. Subsequently, dicoumarol could alleviate KOA in vivo; knee joint diameter was decreased from 11.03 to 9.93 mm. Furthermore, the inflammation and fibrosis of the knee joints were inhibited in rats. In conclusion, the present findings demonstrated that dicoumarol could impede the progression of KOA by inhibiting NLRP3 activation, providing a potential treatment strategy for KOA. [ABSTRACT FROM AUTHOR]

In order to find a new preservation solution for avian coccidial oocysts that can replace potassium dichromate (K2Cr2O7) solution, Eimeria tenella oocysts were preserved in 0.1 to 10% potassium sorbate (C6H7KO2) solution in this study. The results showed that there was no significant difference between the sporulation rate of E. tenella oocysts preserved in 0.1 to 10% C6H7KO2 solution and in 2.5% K2Cr2O7 solution (p > 0.05). The 0.5 to 10% C6H7KO2 solution could also effectively inhibit the growth of bacterial microorganisms. E. tenella oocysts preserved in 1% C6H7KO2 solution at 4 °C for 3, 6, 9, and 12 months, with the oocyst production of E. tenella oocysts being 1.3-, 1.2-, 1.6-, and 1.3-fold higher than that of oocysts stored in 2.5% K2Cr2O7 solution (p < 0.05). In conclusion, C6H7KO2 could replace K2Cr2O7 as the preservation solution of avian coccidial oocysts. [ABSTRACT FROM AUTHOR]

In conventional proportional–integral–derivative (PID) control, the integral term occupies a significant amount of controller memory, which prolongs the calculation time. The integral term easily leads to overshooting and oscillation, while the derivative term reduces the controller's anti-interference ability. In view of these problems, a PID expression with a recurrence relationship was derived, and the integral and differential terms of the conventional PID model were improved using an unsaturated integral and passivation differential to achieve a good control effect. Then, the improved PID was applied to the injection speed control of an injection molding machine, which is usually controlled using conventional PID control that featured difficulty in mathematical modeling, a nonlinear relationship between the input and the output, and high system complexity. Taking an injection molding machine as the control object, the transfer function of the injection system was constructed. Then, the improved PID was simulated using Matlab/Simulink. Lastly, the improved PID was verified using experiments. The simulation and the experimental results showed that the control model had a rapid response, no overshoot, and a high precision. [ABSTRACT FROM AUTHOR]

In conclusion, our study identified two novel loci for hypertension, including SNP rs2064453 in the promoter of I GGT7 i and SNP rs10847208 in lncRNA gene I LINC00943 LINC00944 i . A genome-wide association study identifies novel association between genetic variants in GGT7 and LINC00944 and hypertension Dear Editor, Hypertension affects one billion people in the world.1 Half of the Chinese population aged from 35 to 75 years is also affected with hypertension.2 Genetic factors contribute to hypertension. To determine how risk allele T of rs2064453 increases the expression level of I GGT7 i , we cloned a 2 kb I GGT7 i promoter and regulatory region containing either the C allele or T allele of rs2064453 into the pGL3-basic luciferase reporter vector (Figure 2E). [Extracted from the article]

NOD-like receptors (NLRs) are intracellular sensors of the innate immune system that recognize intracellular pathogen-associated molecular patterns (PAMPs) and danger-associated molecular patterns (DAMPs). Little information exists regarding the incidence of positive selection in the evolution of NLRs of birds or the structural differences between bird and mammal NLRs. Evidence of positive selection was identified in four avian NLRs (NOD1, NLRC3, NLRC5, and NLRP3) using the maximum likelihood approach. These NLRs are under different selection pressures which is indicative of different evolution patterns. Analysis of these NLRs showed a lower percentage of codons under positive selection in the LRR domain than seen in the studies of Toll-like receptors (TLRs), suggesting that the LRR domain evolves differently between NLRs and TLRs. Modeling of human, chicken, mammalian, and avian ancestral NLRs revealed the existence of variable evolution patterns in protein structure that may be adaptively driven. [ABSTRACT FROM AUTHOR]

Background: DNA polymerase β is one of the key enzymes involved in the repair of DNA damage, and its high or low expression is closely related to tumorigenesis. In a previous study on lung cancer, we found three genetic mutations in the promoter region of the Polb gene could be detected in the Han Chinese population. The purpose of this study was to explore the relationship between these mutations and thymic hyperplasia. Methods: Genomic DNA was extracted from 59 thymic hyperplasia patients by the salting out method and used for amplification of the promoter region of the Polb gene. The Polb gene mutation and its frequency were analyzed systematically by comparing them with the deposited wild‐type gene sequence in the NCBI database. The three typical mutated sequences in the promoter region of Polb gene, ‐196G > T, ‐168C > A and ‐188_‐187insCGCCC, were then amplified and ligated to pGL4.10 vector, so as to get the vectors used for the infection of 293T cells to explore their transcription activities by dual‐luciferase reporter system. Results: Two types of mutations, ‐168C>A and‐188_‐187insCGCCC, were found in a significantly higher percentage in patients with thymic hyperplasia than in normal healthy people after sequencing analysis of 59 patients and 60 healthy controls. These results suggest that the two mutations may be closely related to thymic hyperplasia. in vitro functional experiments showed that‐168C>A could significantly increase promoter activity, whereas ‐188_‐187insCGCCC could significantly reduce promoter activity, suggesting that these two mutations may affect the expression level of the Polb gene in cells. Conclusions: Two types of mutations in the promoter region of the Polb gene, ‐168C>A and‐188_‐187insCGCCC, are associated with thymic hyperplasia and may become a new risk factor for this disease. Key points: Significant findings of the study: Genetic mutations in the Polb gene are reported to be associated with different kinds of cancers. However, their relationship with thymic hyperplasia is still unclear. What this study adds: For the first time, we report that two nucleotide mutations in the promoter region of the Polb gene are closely related with thymic hyperplasia after sequencing 59 patients and 60 healthy controls in the Han Chinese population. [ABSTRACT FROM AUTHOR]

Extracellular vesicles (EVs) are membranous structures formed during biological processes in living organisms. For protozoan parasites, secretion of EVs can occur directly from the parasite organellar compartments and through parasite-infected or antigen-stimulated host cells in response to in vitro and in vivo physiological stressors. These secreted EVs characteristically reflect the biochemical features of their parasitic origin and activating stimuli. Here, we review the species-specific morphology and integrity of parasitic protozoan EVs in concurrence with the origin, functions, and internalization process by recipient cells. The activating stimuli for the secretion of EVs in pathogenic protozoa are discoursed alongside their biomolecules and specific immune cell responses to protozoan parasite-derived EVs. We also present some insights on the intricate functions of EVs in the context of protozoan parasitism. [ABSTRACT FROM AUTHOR]

Outbreak of classical swine fever (CSF) results in high mortality and thus causes severe economic losses in the swine industry. Single-domain antibody (sdAb) is the smallest antigen-binding molecule derived from camelid heavy-chain antibodies and has the potential to be used as a molecular probe for detection of CSF virus (CSFV). In this study, two sdAb fragments against the E2 antigen of CSFV were obtained, expressed in vitro. The functional characteristics analysis indicated that the recombinant sdAbE2-1 and sdAbE2-2 have excellent binding activity, specificity, and high affinity with equilibrium constant value of 3.34 × 10−7 and 1.35 × 10−8 M to E2 protein. Then, sdAbE2s were conjugated with quantum dots (QD)/AF488 to synthesize two molecular probes for imaging CSFV distribution in cells. The sdAbE2-1 was also labeled with carboxyl-magnetic beads to construct immunomagnetic nanobeads (IMNBs) able to capture CSFV virions and recombinant E2 protein. QD/AF455-sdAbE2s probes colocalised with CSFV virions in swine testis cells, and IMNBs were used as a detection template and proved to bind specifically with CSFV virions and E2 protein. The selected sdAb fragments and sdAb-based molecular probes may be used for the rapid identification of CSFV during field outbreaks and for research on CSFV and host interactions. [ABSTRACT FROM AUTHOR]

Over the past century, a tremendous amount of work on the Duffing system has been done with continuous external force, including analytical and numerical solution methods, and the dynamic behavior of physical systems. However, hows does the Duffing oscillator behave if the external force is intermittent? This paper investigates the Duffing oscillator with intermittent external force, and a modified Duffing chaotic system is proposed. Different from the continuous-control method, an intermittent external force of cosine function was designed to control the Duffing oscillator, such that the modified Duffing (MD) system could behave chaotically. The dynamic characteristics of MD system, such as the strange attractors, Lyapunov exponent spectra, and bifurcation diagram spectra were outlined with numerical simulations. Numerical results showed that there existed a positive Lyapunov exponent in some parameter intervals. Furthermore, by combining it with chaos scrambling and chaos XOR encryption, a chaos-based encryption algorithm was designed via the pseudorandom sequence generated from the MD. Finally, feasibility and validity were verified by simulation experiments of image encryption. [ABSTRACT FROM AUTHOR]

Mucosal-associated invariant T (MAIT) cells are a subpopulation of evolutionarily conserved innate-like T lymphocytes bearing invariant or semi-invariant TCRα chains paired with a biased usage of TCRβ chains and restricted by highly conserved monomorphic MHC class I-like molecule, MR1. Consistent with their phylogenetically conserved characteristics, MAIT cells have been implicated in host immune responses to microbial infections and non-infectious diseases, such as tuberculosis, typhoid fever, and multiple sclerosis. To date, MAIT cells have been identified in humans, mice, cows, sheep, and several non-human primates, but not in pigs. Here, we cloned porcine MAIT (pMAIT) TCRα sequences from PBMC cDNA, and then analyzed the TCRβ usage of pMAIT cells expressing the TRAV1-TRAJ33 chain, finding that pMAIT cells use a limited array of TCRβ chains (predominantly TRBV20S and TRBV29S). We estimated the frequency of TRAV1-TRAJ33 transcripts in peripheral blood and tissues, demonstrating that TRAV1-TRAJ33 transcripts are expressed in all tested tissues. Analysis of the expression of TRAV1-TRAJ33 transcripts in three T-cell subpopulations from peripheral blood and tissues showed that TRAV1-TRAJ33 transcripts can be expressed by CD4+CD8−, CD8+CD4−, and CD4−CD8− T cells. Using a single-cell PCR assay, we demonstrated that pMAIT cells with the TRAV1-TRAJ33 chain express cell surface markers IL-18Rα, IL-7Rα, CCR9, CCR5, and/or CXCR6, and transcription factors PLZF, and T-bet and/or RORγt. In conclusion, pMAIT cells expressing the TRAV1-TRAJ33 chain have characteristics similar to human and mouse MAIT cells, further supporting the idea that the pig is an animal model for investigating MAIT cell functions in human disease. [ABSTRACT FROM AUTHOR]

This paper addresses the problem of quantized feedback control of nonlinear Markov jump systems (MJSs). The nonlinear plant is represented by a class of fuzzy MJSs with time-varying delay based on a Takagi–Sugeno fuzzy model. The quantized signal is utilized for control purpose and the sector bound approach is exploited to deal with quantization errors. By constructing a Lyapunov function which depends both on mode information and fuzzy basis functions, the reciprocally convex approach is used to derive the criterion which is able to ensure the stochastic stability with a predefined ${l_{2}-l_\infty }$ performance of the resulting closed-loop system. The design of the quantized feedback controller is then converted to a convex optimization problem, which can be handled through the linear matrix inequality technique. Finally, a simulation example is presented to verify the effectiveness and practicability of the proposed new design techniques. [ABSTRACT FROM AUTHOR]

MicroRNAs (miRNAs) are small endogenous non-coding functional RNAs that post-transcriptionally regulate gene expression. They play essential roles in nearly all biological processes including cell development and differentiation, DNA damage repair, cell death as well as intercellular communication. They are highly involved in cancer, acting as tumor suppressors and/or promoters to modulate cell proliferation, epithelial-mesenchymal transition and tumor invasion and metastasis. Recent studies have shown that more than half of miRNAs are located within protein-coding or non-coding genes. Intragenic miRNAs and their host genes either share the promoter or have independent transcription. Meanwhile, miRNAs work as partners or antagonists of their host genes by fine-tuning their target genes functionally associated with host genes. This review outlined the complicated relationship between intragenic miRNAs and host genes. Focusing on miRNAs known as oncogenes or tumor suppressors in specific cancer types, it studied co-expression relationships between these miRNAs and host genes in the cancer types using TCGA data sets, which validated previous findings and revealed common, tumor-specific and even subtype-specific patterns. These observations will help understand the function of intragenic miRNAs and further develop miRNA therapeutics in cancer. [ABSTRACT FROM AUTHOR]

A 19-year-old male with early refractory hypertension, hypokalemia, serum potassium level of 3.4 mmol/l and hypoaldosteronemia was indicated in the present study. According to the results of laboratory tests and examinations, the patient was suspected of having Liddle's syndrome (LS). Genetic analysis of SCNN1B revealed a deletion mutation (c.1721delC). This mutation caused a length extension of SCNN1B coding sequence, which resulted in p.Pro574HisfsX675. A total of 34 family members were enrolled in the study and 29 of these family members underwent genetic testing. A total of 10 family members were clinically diagnosed with hypertension. Notably, 5 family members shared the same gene mutation as the proband and all cases with the mutation had hypertension. Blood pressure of the gene mutation carriers was well controlled by tailored treatment. In conclusion, a patient with early onset and refractory hypertension, hypokalemia and hypoaldosteronemia was diagnosed clinically and genetically with LS. Notably, a novel mutation (c.1721delC) was identified by DNA analysis. The present findings indicate that genetic analysis is useful, not only in the diagnosis of LS, but also in designing a tailored treatment. [ABSTRACT FROM AUTHOR]

Single-domain antibody (sdAb) or nanobody possesses specific features non-accessible for conventional antibodies that make them suitable for research and biotechnological applications. Porcine epidemic diarrhea virus (PEDV) causes lethal diarrhea in piglets, resulting in great economic losses all over the world. To detect and isolate PEDV rapidly and accurately is important for the control and further research of the clinical PEDV strains. In this study, four sdAb fragments (sdAb-Mc19/29/30/37) targeting the membrane (M) protein of PEDV were selected from sdAb library that was constructed through M protein-immunized Camelus bactrianus. The selected sdAb-Mcs were solubly expressed in Escherichia coli. The functional characteristics analysis revealed that the recombinant sdAb-Mcs have excellent binding activity and specificity to M protein but have no neutralizing activity to PEDV. For further application, sdAb-Mc37 was conjugated with quantum dots to synthesize a nanoprobe for imaging PEDV in vero cells. The observed fluorescence in vero cells clearly reflects that PEDV virions can be reliably recognized and labeled by the nanoprobe. Furthermore, the sdAb-Mc29 was conjugated with superparamagnetic nanobeads to construct immunomagnetic nanobeads (IMNBs) used to isolate PEDV. One PEDV strain was successfully isolated from clinical fecal sample, suggesting IMNBs as a novel and efficient tool suitable for PEDV isolation from clinical samples. This study provided a novel application and substantiated the suitability of sdAb as a specific binder for the isolation of viruses. [ABSTRACT FROM AUTHOR]

This paper addresses the problem of dissipativity-based asynchronous control for a class of discrete-time Markov jump systems. A unified framework to design a controller for discrete-time Markov jump systems with mixed time delays is proposed,which is fairly general and can be reduced to a synchronous controller or a mode-independent controller. Based on a stochastic Lyapunov function approach, which fully utilizes available information of the systemmode and the controller, a sufficient condition is established to ensure the stochastic stability and strictly (Q, S, R) dissipative performance of the resulting closed-loop system. Finally, the effectiveness and validity of the proposedmethod are illustrated with a simulation example. [ABSTRACT FROM AUTHOR]