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The effect of tannin-rich fodder (TRF) consumption on the saliva response of Criollo goats without previous browsing experience was determined. Eighteen kids were allocated into three treatments (n = 6 each): control group (CG), short-term tannin stimulus (TS) and long-term tannin stimulus (TL). Three experimental periods were used: adaptation (two weeks) in which the three treatment groups were fedPennisetum purpureumgrass and supplemented with a balanced feed. In period 1 (five weeks), TS and TL treatments were fed TRF (Lysiloma latisiliquum) with 55.5 g/kg DM condensed tannins (CT), grass and balanced feed. In period 2 (three weeks),L. latisiliquumfodder was withdrawn from the TS group. Thus, both the TS and the CG kids were only offered grass and balanced feed, while TL kids continued to receive TRF. In each period, saliva samples were collected to measure the tannin–protein interaction, salivary protein and protein turbidity index (PTI). The salivary protein was similar in all treatments and periods (P > .10). The goats' saliva reacted similarly when mixed with either tannic acid orL. latisiliquumwater-acetone extract. Although the PTI tended to increase in the TL group compared to CG and TS with time, such difference was not significant. Thus, TRF intake failed to further increase the salivary response or PTI. Therefore, the saliva of goats from Yucatan, without previous browsing experience do have a constitutive response as it can block tannins irrespective of TRF stimulus. [ABSTRACT FROM PUBLISHER]

We evaluated the effect of feeding dietary tannins from Lysiloma latisiliquum fresh forage on the saliva tannin-binding capacity of hair sheep lambs without previous exposure to tannin-rich (TR) fodder. Twenty-four hair sheep lambs (13.6±3.04 kg LW) were fed a tannin-free diet at the beginning of the experimental period (from day 10 to 13). On day 14, lambs were distributed into three groups (n=8): control group (CG), fed with the tannin-free diet (from D10 to D112); tannin short-term group (TST), fed the basal diet and 650 g of L. latisiliquum forage (from D14 to D55); tannin long-term group (TLT), fed the basal diet and 650 g of L. latisiliquum forage (from D14 to D112). Saliva samples were collected from the mouth of each lamb in the morning before feeding time on D10 and D14 (baseline period), on D49 and D56 (period 1) and on D97 and D112 (period 2). The tannin binding response of salivary protein (∆% turbidity) was determined with the haze development test (HDT) using either tannic acid or L. latisiliquum forage acetone extract. A turbidity protein index (TPI) was calculated as (∆% turbidity/[salivary protein (mg)]). Differences in HDT and TPI in the different groups were compared by repeated measures ANOVA using Proc Mixed. All groups had similar ∆% turbidity throughout the experiment (P>0.05). At baseline and period 1, the TPI of the different groups was similar (P>0.05). On period 2 the TLT group showed higher TPI compared with CG (P<0.05). Meanwhile, CG and TST showed similar salivary TPI. The saliva of hair sheep lambs consuming TR L. latisiliquum fresh fodder (TLT group) increased their TPI compared with control lambs not exposed to tannins. [ABSTRACT FROM PUBLISHER]