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The timing of nitrogen fertilizer application in vineyards can determine the amount of nitrogen (N) absorbed, distributed, and accumulated in grapevine organs. The study aimed to evaluate the annual contribution of N from urea, applied at different times in Cabernet Sauvignon, grown in sandy and clayey soil in a subtropical climate. The sandy soil received 21.42 kg N ha−1 and the clayey soil 30 kg N ha−1, both enriched with 3% excess 15N atoms, applied at different times. The N derived from the fertilizer in grapevines, at all times of N application, and in sandy and clayey soil did not exceed 8%, with the highest values being observed in annual organs, especially in the leaves. The application of N marked at the phenological stages of IBB (50% at beginning of budbreak + 50% at full budbreak) enabled greater absorption of N derived from the fertilizer by the vines grown in both sandy and loamy soil. The N present in the annual organs (leaves, berries, stalks, and shoots) and in the perennial organs (stems and canes) of Cabernet Sauvignon grown in sandy and clayey soil was derived in greater percentages from the soil. [ABSTRACT FROM AUTHOR]

Assessing the phosphorus (P) balance in agricultural soils is crucial for optimizing its use and reducing contamination risks. The objective of this study was to evaluate the impact of different animal wastes on the distribution of P in soil profiles subjected to 12 years of successive applications of animal wastes and mineral phosphate fertilizer, within a crop rotation system under no-till system. The study was conducted from 2004 to 2016 in the southern region of Brazil. The treatments were the applications of pig slurry (PS), cattle slurry (CS), pig deep-litter (PD), mineral fertilizer (MF), and a control treatment without application. The highest accumulation of P and its movement was observed in the 0–40 cm layer, in the soil submitted to applications of all P sources. The inputs of P via MF, CS, PS, and PD promoted the accumulation of 18, 42, 48, and 100 kg P ha−1 year−1. The P mass balance showed that between 77 and 98% of the P added by animal manure and MF was accounted for in grain exports (17–34%), soil storage (41–72%), and post-harvest residues (<1%), with the remaining 2–33%, unaccounted for, which was attributed, especially, to P transfer at the soil surface. [ABSTRACT FROM AUTHOR]

Copper (Cu) and zinc (Zn) fraction accumulation and adsorption capacity were evaluated in sandy-soil after 16 pig slurry (PS) and deep-litter (DL) applications under subtropical climate. Sixteen PS and DL applications to a Typic Hapludalf increased soil Cu and Zn soluble, exchangeable, mineral, organic, and total fractions. Cu mostly accumulated in organic and residual fractions (1.03, 4.39, 9.81, and 9.68, 12.9, 13.98 mg Cu kg−1 in the control, PS, and DL, respectively), whereas Zn presented a greater increase in mineral and exchangeable fractions (0.42, 6.39, 15.46 and 1.67, 8.96, 14.83 mg Zn kg−1 in the control, PS, and DL, respectively). Manure applications changed Cu and Zn adsorption behavior, with the greatest (895.8 mg Cu kg−1 and 945.9 mg Zn kg−1) adsorption occurring in DL, which is related to the 1.5- and 1.6-times organic matter increase compared to PS and control. Cu showed a preference for soil exchangeable sites under the competitive adsorption system, reducing Zn absorption about 11 and 6 times in the PS and DL, respectively. Application of pig manure rates based solely on crop N demand is unwise as it potentiates Cu and Zn accumulation, Zn bioavailability, increasing the risk of Zn toxicity and transference to aquatic environments. [ABSTRACT FROM AUTHOR]

High soil copper (Cu) concentrations in vineyards can cause phytotoxicity to grapevine rootstocks. In order to mitigate toxicity, the use of grapevine rootstock genetic variation and the application of amendments are possible strategies. The aim of this study is to assess the tolerance of grapevine rootstocks to Cu excess and whether phosphorus (P) and calcium (Ca) can reduce phytotoxicity caused by Cu. Grapevine rootstock seedlings were produced from selected stakes: Paulsen 1103 (Vitis berlandieri × Vitis rupestris); SO4 (Vitis berlandieri × Vitis riparia); IAC 572 ((Vitis Riparia × Vitis rupestris) × Vitis caribaea); and Isabel (Vitis labrusca). Seedlings were grown in nutrition solution added with the following treatments: 0.3 µM Cu (control); 60 µM Cu; 60 µM Cu and 62 mg L−1 P; 60 µM Cu and 400 mg L−1 Ca. High Cu concentration caused phytotoxicity in all rootstocks, impairing their growth and decreasing nutrient concentration and photosynthetic activity. P and Ca addition had positive effect on the photosynthetic activity of all rootstocks, although it was not enough to revert growth to levels comparable with controls. Overall, based on the results, the application of P and Ca was not efficient in mitigating Cu phytotoxicity in grapevine plants grown in solution. Isabel was the most sensitive rootstock to Cu phytotoxicity, whereas Paulsen 1103 and SO4 presented more tolerance and can be used, together with other management strategies, in contaminated vineyard areas. Therefore, careful genotype rootstock selection for use in high Cu soils is important, while Ca and P are not efficient mitigators of Cu toxicity. [ABSTRACT FROM AUTHOR]

Vineyard soils normally do not provide the amount of nitrogen (N) necessary for red wine production. Traditionally, the N concentration in leaves guides the N fertilization of vineyards to reach high grape yields and chemical composition under the ceteris paribus assumption. Moreover, the carryover effects of nutrients and carbohydrates stored by perennials such as grapevines are neglected. Where a well-documented database is assembled, machine learning (ML) methods can account for key site-specific features and carryover effects, impacting the performance of grapevines. The aim of this study was to predict, using ML tools, N management from local features to reach high berry yield and quality in 'Alicante Bouschet' vineyards. The 5-year (2015–2019) fertilizer trial comprised six N doses (0–20–40–60–80–100 kg N ha−1) and three regimes of irrigation. Model features included N dosage, climatic indices, foliar N application, and stem diameter of the preceding season, all of which were indices of the carryover effects. Accuracy of ML models was the highest with a yield cutoff of 14 t ha−1 and a total anthocyanin content (TAC) of 3900 mg L−1. Regression models were more accurate for total soluble solids (TSS), total titratable acidity (TTA), pH, TAC, and total phenolic content (TPC) in the marketable grape yield. The tissue N ranges differed between high marketable yield and TAC, indicating a trade-off about 24 g N kg−1 in the diagnostic leaf. The N dosage predicted varied from 0 to 40 kg N ha−1 depending on target variable, this was calculated from local features and carryover effects but excluded climatic indices. The dataset can increase in size and diversity with the collaboration of growers, which can help to cross over the numerous combinations of features found in vineyards. This research contributes to the rational use of N fertilizers, but with the guarantee that obtaining high productivity must be with adequate composition. [ABSTRACT FROM AUTHOR]

The nitrogen (N) critical level in leaves and maximum technical efficiency (MTE) doses contribute to the grape yield, must quality, and fertilizers rationalization. This study aimed to define sufficient ranges and critical levels in leaves and maximum technical efficiency doses to obtain high yields and quality must in grapevines grown in sandy soil in a subtropical climate. 'Chardonnay' and 'Pinot Noir' grapevines were subjected to the annual application of 0, 10, 20, 40, 60, and 80 kg N ha−1 in a vineyard. The nitrogen concentration in leaves at flowering and veraison, grape yield and grape must chemical parameters (total soluble solids—TSS, total titratable acidity—TTA and total anthocyanins—TA) were evaluated. The N critical level in leaves at flowering was different between grapevines cultivars, but this was not observed in leaves at veraison. It was possible to estimate MTE for 'Chardonnay' grapevines, in the evaluated growing seasons. In the range of higher probability of the N critical level in leaves, the TSS and TTA variables showed opposite responses, for both cultivars. This study proposes different N critical levels for red and white grapevines, in a subtropical climate. [ABSTRACT FROM AUTHOR]

Grass species native to South American can have mechanisms to tolerate copper (Cu) excess, which improves their use to phytoremediate Cu-contaminated soils. The aims of the present study are to assess the tolerance of grass species native to South American grasslands to copper-contaminated soils, as well as their adaptive responses under high Cu-stressed condition and to identify native grass species presenting the highest potential to be used for phytoremediation purposes. Soil samples were air-dried and their acidity, phosphorus and potassium levels were corrected, and the samples were incubated. Three Cu levels were used in the experiment: natural (Dose 0), with added of 40 mg kg−1 of Cu and with added of 80 mg kg−1 of Cu. Three Axonopus affinis, Paspalum notatum and Paspalum plicatulum seedlings were transferred to 5-L pots filled with soil in August and grown for 121 days. Soil solution was collected during cultivation with the aid of Rhizon lysimeters. Main concentrations of cations and anions, dissolved organic carbon and pH in the soil solution were analyzed and the ionic speciation was carried out. Cu toxicity impaired the growth of grass species native to South America, since Cu excess led to both changes in root morphology and nutritional unbalance. Among all assessed native species, Paspalum plicatulum was the one presenting the greatest potential to phytostabilize in Cu-contaminated soils, since it mainly accumulates Cu absorbed in the roots; therefore, its intercropping with grapevines is can be beneficial in Cu-contaminated soils. [ABSTRACT FROM AUTHOR]

Study's objective was to evaluate spatial variability of herbaceous cover species community in vineyards cultivated in soil with increasing Cu levels in Pampa biome. Three vineyards, with increasing soil Cu available contents and a natural field area (NF), were selected. In each experimental area, soil Cu content, botanical composition, cumulative aerial biomass, and aerial part Cu concentration, in most frequent species, were evaluated. In total, 39 vascular plant species were identified, including four exotic species. Biodiversity indicators did not significantly correlate with soil Cu. However, botanical composition variation could be observed. In NF, Poaceae and Asteraceae families presented greater dry mass contribution, while this contribution decreased in higher soil Cu concentration areas. The Cu concentration and accumulation in plant aerial part were higher in older vineyards, as plant aerial part accumulated, in average, 13.8 mg Cu m−2. Among species found in experimental fields, Ageratum conyzoides, a species known to form Cu-tolerant populations, occurred in most areas, especially in vineyards, presenting higher aerial Cu concentrations, with a mean of 126.47 mg kg−1. Soil enrichment with Cu did not alter the vegetation's biodiversity, but may have contributed to the botanical composition modification. The native species, P. plicatulum and A. conyzoides, presented a high bio-accumulation factor and are potential candidates for phytoremediation techniques. [ABSTRACT FROM AUTHOR]

Fruit and vegetable waste can be used for the production of organic compost, which when mineralized can increase the availability of nitrogen. The objectives of this study were: (a) to produce compost from different ratios of fruit and vegetable waste, rice husk and poultry manure that meets the legislation on organic fertilizers and (b) to assess the mineralization of N in substrates prepared with different ratios of compost. In experiment 1, the following treatments were prepared using (fruit and vegetable residue: rice husk: poultry waste) (v:v): Treatment 1 (2:1:0), T2 (1:1:1), T3 (1.5:1:0) and T4 (1.2:1:0). All the treatments were subjected to composting for 95 days, were subjected to analysis of nutrients, organic carbon, C/N, CEC/C, pH and moisture content. The composts were compared in regards to the parameters required by legislation. In experiment 2, the treatments consisted of eight blends of agricultural peat, carbonized rice husk and organic compost pre-selected from experiment 1. The NO3 --N and NH+ 4-N contents were analyzed in the substrate at time zero and at 7, 14, 28, 56, 112 days after incubation. Compost (C2) met the parameters required by legislation and the use of 40% in the substrate composition promoted the mineralization of N. [ABSTRACT FROM AUTHOR]

Forest species Angico-Vermelho (Parapiptadenia rigida (Bentham) Brenan) is an alternative for the revegetation of areas contaminated with high levels of heavy metals such as copper (Cu). However, excess Cu may cause toxicity to plants, which is why the use of soil amendments can facilitate cultivation by reducing the availability of Cu in the soil. The aim of this study was to assess how the use of amendment can contribute to growth and nutritional status as well as reduce oxidative stress in Angico-Vermelho grown in Cu-contaminated soil. Samples of a Typic Hapludalf soil containing high Cu content were used for the application of four amendments (limestone, organic compost, Ca silicate and zeolite), in addition to a control treatment. The treatments were arranged in a completely randomized design, with four replicates. The use of amendments decreased Cu content available in soil and contributed to improve both plant nutritional status and its antioxidant response expressed by enzymatic activity. The application of the amendments, especially zeolite and Ca silicate, increased dry matter yield of Angico-Vermelho. Thus, the results presented here suggest that the use of amendments contributes to improving Cu-contaminated soils and favors revegetation with Angico-Vermelho. [ABSTRACT FROM AUTHOR]

The use of copper (Cu)-based fungicides to control diseases in vineyards can cause excessive Cu accumulation in soils. Greater Cu availability is expected in acid sandy soils with low organic matter (OM), where the risk of toxicity to plants and environment contamination is high. This study aimed to study the evolution of Cu in acid, sandy soils planted with grapevines for a period of time between 2 and 32 years. Two studies were carried out in South Brazil: in the first study, soil samples, from 19 vineyards were collected and analyzed in the first 0.2 m depth-layer for the total and available Cu. In the second study, 3 soils were selected among the previous 19 according to the age of vineyard (5, 11, and 30 year-old), and their 0.60 m-depth profile was analyzed for 5 fractions of Cu (soluble, exchangeable, mineral, organic, and residual). All the soils were classified as Ultisols. The vineyards with more than 25 years under cultivation had a mean total Cu concentration of 90 mg kg−1in the 0–0.10 m layer. Approximately 80% of it was potentially available to plants. The greatest total and available Cu were found in the soil surface layers (0.2 m depth), where up to 75% of the total Cu is adsorbed in clay minerals and only 20% is complexed in the OM. [ABSTRACT FROM PUBLISHER]

The purpose of this study was to evaluate the mobility of copper (Cu) and zinc (Zn) in the profile and distribution of sandy soil fractions from vineyards. Soil samples were collected from two vineyards of different ages (14 and 30 years) and a natural field area in the south of Brazil. The chemical characteristics, Cu levels and Zn levels, were analyzed in the soils by extraction with ethylenediamine tetraacetic acid (EDTA) using the 3050B method of USEPA and by chemical fractionation. Cu and Zn were accumulated in the vineyard soils, especially in the uppermost soil layers and in the soil from the oldest vineyard. Approximately, 75% of the total Cu was extracted by EDTA, but only 30% of the total Zn was extracted by EDTA. Most of the Cu, especially in the oldest vineyard, was distributed in the mineral-associated fraction, which is characterized by low geochemical mobility, but another important part of the Cu was distributed in the soil organic matter. Most of the Zn in the soil was distributed in the residual and mineral-associated fractions, which are characterized by low mobility and have potential for causing toxicity to plants. [ABSTRACT FROM AUTHOR]

No-till farming and manure application are means of storing carbon in soil and increasing soil quality. However, the organic fertilization of no-till soils may sustain subtropical agroecosystems for only a limited period until the soil quality declines. The C, N, P, and other nutrient cycles are perturbed due to the nutrient imbalance in manures and their application on the soil surface, which poses a risk for nutrient dispersion into the environment. Long-term N-based manure application also impacts the apparent nutrient recovery (ANR) by crops and, hence, the crop quality. Our objective was to monitor changes in soil quality and to measure nutrient recovery from N-based organic fertilization in a Brazilian agroecosystem. A long-term experiment was established in May 2004 on Typic Hapludalf soil in Southern Brazil. The experiment comprised two periods: 2004–2009 and 2009–2020. The treatments were unfertilized (control), pig slurry, pig deep litter (swine manure and rice husk), cattle slurry, and mineral fertilizer (urea, triple superphosphate, and K chloride). Grain and cover crops were grown. The maize grain yield and oat biomass were used as indicators of productivity. The application of cattle slurry, pig deep litter, and pig slurry increased the grain production by 20% on average compared to mineral fertilization. Organic manuring increased the soil pH by up to one unit, while urea acidified the soil. The highest crop yields were obtained with pig deep litter, which increased the levels of P, K, Ca, Mg, and C in the soil test. Pig deep litter showed the highest N-ANR for the aboveground part of the plants. The ANRs for P, K, Ca, and Mg were also highest when pig slurry had been applied. The ANR values for the micronutrients varied widely among the organic sources but indicated large marginal offtake gains compared to mineral fertilization. The N-based fertilization with animal manures increased the rate of nutrient uptake by plants but led to an accumulation of nutrients in the soil, threatening the sustainability of the system. Manure applications must be monitored regularly to avoid the accumulation of nutrients that could impair the soil quality and become an environmental problem in the future. [ABSTRACT FROM AUTHOR]

The purpose of this study was to quantify the phosphorus (P) fractions in phosphate-fertilized sandy soils under grapevines. Soil was sampled from a grassland site and two vineyards (13 and 31 years old) in the State Rio Grande do Sul, Brazil, from the layers 0–5, 5–10, 10–15, 15–20, and 20–40 cm deep. The samples were prepared and P fractions were assessed by chemical fractionation. Phosphate fertilization of the vineyard soils increased P contents to a depth of 40 cm, especially in the more recalcitrant fractions but detectable in the more labile fractions as well, which are plant available and may increase the risk of environmental contamination. Phosphate fertilizers and the period of grapevine cultivation had little effect on the levels of organic P forms, whereas cover crops in vineyards could be an appropriate strategy to increase or maintain the levels of soil organic matter and the levels of organic P over the years. [ABSTRACT FROM AUTHOR]

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