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Five new steroidal glycosides, timosaponin J (1), timosaponin K (2), (25S)-karatavioside C (5), timosaponin L (6), and (25S)-officinalisnin-I (8), together with eight known steroidal saponins, timosaponin E1 (3), purpureagitosid (4), timosaponin BII (7), timosaponin B III (9), anemarrhenasaponin I (10), anemarrhenasaponin III (11), anemarrhenasaponin A2 (12), and timosaponin A III (13), were isolated from the rhizomes of Anemarrhena asphodeloides. Their structures were elucidated on the basis of spectroscopic and chemical evidence. The aglycones of compounds 1 and 2 are new aglycones. Compounds 1-13 were evaluated for their platelet aggregation activities, and compound 13 exhibited the strongest inhibitory effect on adenosine diphosphate (ADP)-induced platelet aggregation. [ABSTRACT FROM AUTHOR]

Nine spirostanol saponins (1-9) and seven mixtures of 25R and 25S spirostanol saponin isomers (10-16) were obtained from the seeds of Trigonella foenum-graecum after enzymatic hydrolysis of the furostanol saponin fraction by β-glucosidase. Their structures were determined by NMR and MS spectroscopy. Among them, 1-4, 6, 8, and 9 were new compounds and five, 11b, 12a, 13b, 14a, and 14b, were new structures observed from seven mixtures. In addition, the inhibitory effects of all saponins on rat platelet aggregation were evaluated. [ABSTRACT FROM AUTHOR]