1. Development of affinity-based delivery of NGF from a chondroitin sulfate biomaterial.
- Author
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Butterfield, Karen Chao, Conovaloff, Aaron W., and Panitch, Alyssa
- Subjects
HEALTH outcome assessment ,BIOMATERIALS ,CHONDROITIN sulfates ,NERVE growth factor ,NERVE tissue proteins ,NEUROTROPHINS - Abstract
Chondroitin sulfate is a major component of the extracellular matrix in both the central and peripheral nervous systems. Chondroitin sulfate is upregulated at injury, thus methods to promote neurite extension through chondroitin sulfate-rich matrices and synthetic scaffolds are needed. We describe the use of both chondroitin sulfate and a novel chondroitin sulfate-binding peptide to control the release of nerve growth factor. Interestingly, the novel chondroitin sulfate-binding peptide enhances the controlled release properties of the chondroitin sulfate gels. While introduction of chondroitin sulfate into a scaffold inhibits primary cortical outgrowth, the combination of chondroitin sulfate, chondroitin sulfate-binding peptide and nerve growth factor promotes primary cortical neurite outgrowth in chondroitin sulfate gels. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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