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Lepidium ostleri S.L. Welsh & Goodrich (Ostler's peppergrass) is an endemic plant species restricted to Ordovician limestone outcrops associated with the San Francisco Mountain Range in western Utah. Due to restricted population distribution and proximity to modern mining operations, L. ostleri is a species of conservation interest. This study focused on the development of a micropropagation protocol for propagating mature plants using plant tissue culture methods. Indirect shoot organogenesis was obtained from L. ostleri explants on Murashige and Skoog (MS) medium augmented with various concentrations of BAP (6-Benzylaminopurine), kinetin (N6-furfuryladenine), and IAA (indole-3-acetic acid). Plantlets supporting shoots grown in vitro were pulse treated with differing strengths of indole-3-butyric acid (IBA) and transferred to sterile soil. Following root induction, plantlets were acclimated to ambient conditions. The successful development of a micropropagation protocol supports management activities for L. ostleri and also contributes to in vitro propagation knowledge at the species, genus, and family levels. [ABSTRACT FROM AUTHOR]

In this project we developed a successful methodology for producing seeds of a federally listed endangered species, Holmgren's milkvetch (Astragalus holmgreniorum Barneby [Fabaceae]), in container culture. The goal was to obtain seeds for augmentation and introduction studies aimed at species recovery. Once requirements for germination, establishment, healthy growth, and flowering were determined, this perennial, spring ephemeral species could readily be grown from seed to flowering in the greenhouse on a fall-to-spring production cycle. Plants required several weeks of cold treatment (vernalization) in midwinter to induce flowering. We also succeeded in carrying container-grown plants through their first dormant season to obtain a second year of seed production. The main obstacle to successful seed production was matching flowering time and place to coincide with the activity of the ground-nesting native bees (genus Anthophora) that are its principal pollinators. Most of the nearly 44,000 seeds produced by approximately 125 greenhouse-grown plants over 2 y resulted from hand-pollination, which was very effective but labor intensive. Yield from hand-pollination averaged 436 seeds per plant under the best scenario, which compares favorably with the yield for wild plants. We obtained fruit set without hand-pollination only when we placed flowering container-grown plants in the field where wild Holmgren's milkvetch was flowering and Anthophora bees were present. This field method could be scaled up and would result in large seed crops without the need for hand-pollination. Scaling up seed production in the absence of the bees proved to be impractical. [ABSTRACT FROM AUTHOR]

Arctomecon humilis (dwarf bear poppy) is an endangered gypsophile restricted to the St. George area in southern Utah, USA. Extirpation of the rare oligolectic bee Perdita meconis and decline of other native pollinators within the last 20 years has been largely attributed to the advent of invasive Africanized honeybees (AHBs), which have become the predominant pollinator for A. humilis. A three-year study was carried out to determine if reliance on AHBs had a negative effect on reproductive output. In the first year, pollen addition experiments were carried out in a small, isolated population to determine if pollinator scarcity or genetic factors affected reproductive output. In the subsequent two years, reproductive output studies were conducted across four populations. In all years, fruit set across study sites was exceptionally high. There was no consistent effect from flower number or interplant distance, which have historically been shown to have a significant impact on reproductive output prior to AHB arrival. The consequences and sustainability of this relatively young relationship between A. humilis and the AHB remain unclear. However, high reproductive success shows that the AHB is currently capable of adequately fulfilling pollinator services for A. humilis. [ABSTRACT FROM AUTHOR]