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Charcoal is commonly preserved in both natural and artificial sediments, and is intensively used in paleontological, paleoenvironmental, and archaeological studies due to the abundant bio-information it contains. The biochemical properties of charcoal are also used for paleoclimatic reconstruction; however, the reliability of this approach has been challenged due to a lack of clarity on how physicochemical properties change during the charring process, as well as the temperatures required for charcoalification. To address this lack, in this study, Qinghai spruce and Chinese pine wood samples from the northeastern Tibetan Plateau were heated at different temperatures and for different lengths of time under restricted oxygen conditions. The reflectance; carbon, nitrogen, and oxygen content; and tracheid morphology were quantified before and after heating to assess changes related to the charring process. Archaeological charcoal remains were then evaluated to determine the charcoalification temperatures by comparing with the experimental results. The minimum temperature required for wood charcoalification was ~300 ℃, while temperatures recorded by archaeological charcoal were concentrated at 400-500 ℃. During the charring experiments, the tracheid cell walls gradually homogenized, and tracheid cell wall thickness and lumen area decreased by ~20%. On average, 50% mass losses were observed; the carbon and oxygen content (% wt.) approximately changed from 47% to 60% and 48% to 35% respectively, while the nitrogen content (% wt.) fluctuated around 0.2%. The reflectance increased slightly from 0% to 0.5%. We propose that the charcoalification of wood tissue refers to charring (in restricted air) and carbonization (in the almost absence of air) when the wood is exposed to a heat source, which then finally transforms into a black, inert solid. This quantitative study provided valuable data and a thorough assessment of the process of wood charcoalification, as well as accurately estimated the feasibility of using charcoal physicochemical properties in paleoclimatic research. [ABSTRACT FROM AUTHOR]

The history of fruit-tree utilization by prehistoric people has become an important issue that has attracted increasing attention in recent years. However, the question of how people used fruit trees has not yet been answered; in particular, the impacts of different subsistence strategies on human behavior regarding fruit-tree utilization (wild gathering or conscious cultivation) have not yet been considered. Here, we present the results of charcoal identification of fruit trees from 16 dated archeological sites in the northeastern Tibetan Plateau (NETP) spanning the period c. 5,200–2,600 BP. We combine this with reported multidisciplinary evidence to explore the history of fruit-tree utilization as well as its relation to the subsistence strategy in the NETP during the late Neolithic and Bronze Age. Our results demonstrate that Rosaceae [Prunus L., Prunus Padus L., Maloideae L., and Malus baccata (L.) Borkh], Elaeagnaceae (Hippophae L. and Elaeagnus angustifolia L.), and Rhamnaceae (only Ziziphus Mill.) were used by people in the NETP, and there was a downward trend in the use of fruit trees during the late Neolithic and Bronze Age. This is in notable contrast to the situation in the Chinese Loess Plateau in the parallel period. The cold-dry climate during the Bronze Age seemed to be one of the reasons. The fruit trees used by people in the NETP were likely gathered from the wild rather than consciously cultivated, and the subsistence strategy of agropastoralism may have played a significant role during the processes. [ABSTRACT FROM AUTHOR]