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Introduction The JAK2V617F mutation has emerged in recent years as a diagnostic as well as a treatment target in patients with polycythemia vera ( PV) and essential thrombocythemia ( ET). The disease phenotype is also influenced by other factors such as micro RNA (mi RNA) deregulation. The aim of this study was to investigate miR-125 expression level in these patients with those obtained from healthy control subjects and its correlation with JAK2 allele burden and laboratory findings. Methods In total, forty patients with a clinical diagnosis of PV and ET were examined at the time of diagnosis. Ten healthy subjects were checked as controls. We performed JAK2 V617F allele burdens measurement and expression analysis of miR-125b-5p, miR-125b-3p, miR-125a-5p, and miR-125a-3p in leukocytes isolated from peripheral blood by quantitative real-time polymerase chain reaction. Results MiR-125b-5p and miR-125a-5p were upregulated in both patients with PV ( P = 0.00 and P = 0.003, respectively) and ET ( P = 0.02 and P = 0.002, respectively). In PV group, a significant correlation was observed between miR-125a-5p and platelet counts ( P = 0.01, r = 0.531). The correlation between mi RNA and JAk2 allele burden was not significant. Conclusion In conclusion, our data indicate that other factors such as aberrant miR-125 expression may influence on the disease phenotype in patients with PV and ET. [ABSTRACT FROM AUTHOR]

In this study, poly(ethylene terephthalate) (PET) fabrics were modified with two types of commercial lipases, namely, Lipex and Lipolase, and grafted with acrylic acid (AA) to improve their absorption properties. The effects of the enzyme concentration, reaction temperature, time, and pH on the grafting of AA onto PET were investigated. The pretreatment of PET with lipases increased the amount of AA that was introduced to the PET fibers, whereas AA grafting onto the untreated PET fabrics led to lower graft yields. Fourier transform infrared spectroscopy and scanning electron microscopy were used to characterize the AA-grafted pretreated polyester fabrics. A new band appearing at 1546 cm-1 in the Fourier transform infrared spectrum implied that AA was introduced onto the PET fabrics. The surfaces of the fabric fibers presented in scanning electron microscopy micrographs clearly indicated the formation of a layer of grafted poly (acrylic acid). The results show that the density of surface grafting was improved by the lipase pretreatment. The increase in grafting was higher for Lipex than for Lipolase. The highest graft yield was obtained with 1% Lipex and Lipolase for 30 min at pH values of 7 and 5, respectively. There were no significant changes in the tenacity or weight reduction of the fabrics. The moisture content of the samples increased linearly with increasing graft yield. This was higher for the pretreated fabrics grafted with Lipex. A higher color strength was obtained for grafted PET samples that were pretreated with Lipex when they were dyed in alkaline aqueous solutions. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010 [ABSTRACT FROM AUTHOR]