Search

The use of biocides is one of the main threats to biodiversity worldwide, representing a significant driver of decline for several taxa. Mechanical devices for the control of pest insect populations are commonly considered as less impacting, and yet may also represent a serious threat to wildlife. Among such mechanical devices, adhesive traps meant to control pest insects are especially widespread, and anecdotal evidence suggests that several vertebrates are at risk of being trapped by adhesive traps as bycatch. Specifically, we here focus on bats as potential bycatch of adhesive traps across Europe, conducting a literature review and community science data collection to describe and quantify bat mortality due to such devices, followed by a trait-based approach to highlight potential biases in the frequency of bycatch. We retrieved 222 cases of bats caught by adhesive traps, involving 17 species, with the brown long-eared bat Plecotus auritus being the most heavily affected. Among European bat species, probability of being caught by adhesive traps is significantly trait-biased, with the risk of getting caught being higher for species featuring smaller size, a gleaning hunting style, and being associated with anthropogenic habitats. Our results shed light upon a so-far overlooked risk posed by mechanical remedies meant to control insect pests upon bats, and the ecosystem services they provide. Our findings thus clearly indicate that the use of such devices should be avoided, especially in proximity to important roosts of rare or sensitive species, besides providing insights into the risk of “eco-friendly” remedies in organic agriculture. [ABSTRACT FROM AUTHOR]

Traditional farming, where livestock is seasonally managed as free ranging and the use of drugs is reduced or absent, may prove beneficial to biodiversity by fostering the occurrence of spatial heterogeneity, and increasing the availability of trophic resources to wildlife. Previous work indicates that the presence of cattle in lowlands leads to an increase in bat foraging activity, yet no study has addressed this topic in mountainous regions, where free-ranging livestock is still common. Here we explore the relationships between landscape structure, farming and bat activity in a mountainous agricultural area, hypothesizing that bat activity will increase in response to the presence of livestock and landscape structure and heterogeneity. We found that traditional cattle farming may have a role in influencing bat activity in mountainous agroecosystems, yet its effects are evident for a limited number of species. Three pipistrelle species favoured foraging in areas subjected to cattle farming by hunting more often over cattle or fresh dung than at control sites. Free-ranging cattle thus provide profitable foraging opportunities for bats in mountainous landscapes, which remarks the importance of traditional farming activities in sustaining biodiversity. Cattle might also benefit from bat foraging activity if this leads to suppression of bloodsucking pests. [ABSTRACT FROM AUTHOR]

Purpose: Abdominal wall hernia is one of the commonest surgical disorders worldwide, and there is no single gold-standard operative technique to repair it. In an effort to improve techniques and technologies to reinforce hernia repair, synthetic meshes are employed. In this study, a new prosthesis (named composite) formed of two polypropylene layers, one macroporous (named mesh) and one transparent (named film), was examined to evaluate its capability to enable cell proliferation without inducing cell death. Inflammatory processes were also examined. Methods: Human fibroblasts BJ were seeded on multiwells, on which composite or film had been placed. After 7, 14, and 21 days, cell growth and viability, deposition of collagen, and release of IL-6, IL-1β, and TNF-α were evaluated. Results: The 'in vitro' protocol showed the composite to be colonized by human fibroblasts on the polypropylene macroporous mesh side; no cell growth occurred on the film. The slowdown of cell growth observed between 14 and 21 days was accompanied by an increase in type I collagen deposition and marked fibroblast activity. Inflammatory cytokines initially increased, followed by their reduction beginning at 14 days. Conclusions: The new prosthesis comprising two polypropylene layers of differing morphologies can be colonized by fibroblasts on the side facing the abdominal wall, whereas no cell growth occurs on the side facing the viscera. The transient inflammation, observed at early experimental times, is probably important for the healing process. [ABSTRACT FROM AUTHOR]

This study was designed to investigate the attitude of a positioner (Finition©) or an Hawley’s retention appliance to create a symmetric function of the masticatory, neck and trunk muscles during the orthodontic retention phase. Sixteen patients (18·5 ± 2·2 years) were included in the sample. All the patients received an orthodontic therapy for the treatment of an Angle’s class I malocclusion and skeletal class I, for a period of 2 years. After removal of orthodontic brackets, eight patients wore a teeth positioner (group I) as retention appliance and eight subjects wore an Hawley’s appliance as upper retention (group II). Surface electromyographic (sEMG) activity was recorded from the bilateral anterior temporal, posterior temporal, masseter, sternocleidomastoid (SCM), upper and lower trapezius and cervical muscles at mandibular rest position and during maximal voluntary clenching (MVC) at T0, i.e. soon after the removal of orthodontic brackets and at T1, i.e. after 3 months of retention treatment. No significant difference was observed between the two groups at T0. At T1, at mandibular rest position, the group I showed a significantly lower sEMG activity of anterior temporal and SCM muscles compared with the group II. During MVC, at T1, the group I showed a significantly lower sEMG activity of the SCM and posterior cervicals, compared with the group II. The positioner seems to show a relevant effect on the sEMG activity of masticatory, neck and trunk muscles. [ABSTRACT FROM AUTHOR]

The aim of this study was to evaluate the association between cervical posture on lateral skull radiographs and surface electromyographic recordings (sEMG) of head and neck muscles. The sample comprised 40 Caucasian adult females, average age 26·8 (20–48); lateral skull radiographs were obtained in natural head position (mirror position). sEMG activity was bilaterally investigated for the following muscles: masseter, anterior temporalis, digastric, posterior cervical, sternocleidomastoid and upper and lower trapezius. All muscles were monitored at rest and during maximal voluntary clenching (MVC). A Pearson’s correlation coefficient revealed significant correlations ( P < 0·01) between cranio-cervical angulations and sEMG activity of masseter, digastric, lower trapezius, during MVC and anterior temporalis at rest. Significant correlations ( P < 0·01) were also found between cervical lordosis angle and sEMG activity of masseter (during MVC) and lower trapezius (at rest). In view of transversal method, no conclusion was possible about the mechanism concerning these results. Future longitudinal studies should be directed to understand the extent of environmental and genotype influences by masticatory muscle activity on cervical posture. [ABSTRACT FROM AUTHOR]

The electromyographic pattern activity of masticatory, neck and trunk muscles was assessed using surface electromyography (sEMG) in 60 Caucasian adult females (20 subjects in skeletal class I, 20 subjects in skeletal class II and 20 subjects in skeletal class III), classified on the base of their skeletal class (ANB angle), corrected on the base of maxillary and mandibular rotations. The sEMG activity was recorded at mandibular rest position and during maximal voluntary clenching. At mandibular rest position, the sEMG activities of masseter and anterior temporal muscles were significantly higher in class III subjects than in class I and class II subjects, that showed no significant difference between them. Then, the sEMG activities of posterior cervicals and upper trapezius were significantly higher in skeletal class III subjects than in the other two groups. During maximal voluntary clenching, no significant difference was observed in the sEMG activity of masticatory muscles among the three considered groups. However, the sEMG activities of posterior cervicals and upper trapezius were significantly higher in skeletal class III subjects than in the other two groups, which showed no significant difference between them. In conclusion, the skeletal class seems to affect the sEMG pattern activity of masticatory, neck and trunk muscles. [ABSTRACT FROM AUTHOR]

The electromyographic pattern activity of masticatory, neck and trunk muscles was assessed using surface electromyography (sEMG) in 60 Caucasian adult females (20 subjects in skeletal class I, 20 subjects in skeletal class II and 20 subjects in skeletal class III), classified on the base of their skeletal class (ANB angle), corrected on the base of maxillary and mandibular rotations. The sEMG activity was recorded at mandibular rest position and during maximal voluntary clenching. At mandibular rest position, the sEMG activities of masseter and anterior temporal muscles were significantly higher in class III subjects than in class I and class II subjects, that showed no significant difference between them. Then, the sEMG activities of posterior cervicals and upper trapezius were significantly higher in skeletal class III subjects than in the other two groups. During maximal voluntary clenching, no significant difference was observed in the sEMG activity of masticatory muscles among the three considered groups. However, the sEMG activities of posterior cervicals and upper trapezius were significantly higher in skeletal class III subjects than in the other two groups, which showed no significant difference between them. In conclusion, the skeletal class seems to affect the sEMG pattern activity of masticatory, neck and trunk muscles. [ABSTRACT FROM AUTHOR]

The aim of the study was to evaluate the effects that an anterior cruciate ligament injury of the knee has on postural control and activity of neck, head and trunk muscles to investigate the existence of connections between the masticatory system and body posture. Surface electromyographic activity of the muscles at mandibular rest position, and during maximal voluntary clenching and posturometric and stabilometric measurements of 25 adult patients having pathology on the left knee were compared with a control non-pathological group. At rest, the patients showed a higher muscular activity of anterior temporalis, masseter, sternocleidomastoid and lower trapezius, compared with the control subjects ( P < 0·05). At maximal voluntary clenching, the patients showed a lower muscular activity of the right anterior temporalis and masseter and a higher muscular activity of the lower trapezius, compared with the control subjects. For the stabilometric measurements, all the subjects showed a significant reduction in the postural centre of pressure path length during the test with eyes open and cotton rolls, compared with the test with eyes closed and mandibular rest position ( P < 0·05). In addition, the patients showed a significant displacement of the postural centre of pressure in a forward direction ( P < 0·05) and into the right side ( P < 0·05), compared with the control subjects. Anterior cruciate ligament injury appears to be associated to a change in the activity of head, neck and trunk muscles and to a change in the position of the postural centre of pressure. Cotton rolls seem to improve the stability of the subject. [ABSTRACT FROM AUTHOR]

To evaluate caste-biased gene expression in Melipona quadrifasciata, a stingless bee, we generated 1278 ESTs using Representational Difference Analysis. Most annotated sequences were similar to honey bee genes of unknown function. Only few queen-biased sequences had their putative function assigned by sequence comparison, contrasting with the worker-biased ESTs. The expression of six annotated genes connected to caste specificity was validated by real time PCR. Interestingly, queens that were developmentally induced by treatment with a juvenile hormone analogue displayed an expression profile clearly different from natural queens for this set of genes. In summary, this study represents an important first step in applying a comparative genomic approach to queen/worker polyphenism in the bee. [ABSTRACT FROM AUTHOR]

The purpose of this study was to evaluate the activity of digastric, sternocleidomastoid, upper trapezius, lower trapezius and cervical muscles in response to maximum voluntary clenching (MVC) of the teeth. Twenty healthy subjects aged 24–31 years were divided into two groups of 10: a resting group and a clenching group. The resting group simply maintained the mandible at rest with teeth apart for two 1-min periods, whereas the clenching group underwent an initial resting period followed after 1-min by a clenching period during which they were required to perform MVC. The electromyography (EMG) responses of each muscle were recorded during all periods, and means and standard deviations of the root mean square EMG were calculated. Within-group and between-group comparisons of EMG activity were made using, respectively, the Sign two-tailed test for two related samples and the Kolmogorov–Smirnov two-tailed test for two independent samples (significance level 0·05). Data indicated a significant increase of EMG activity of the anterior neck muscles (digastric and sternocleidomastoid) during MVC as compared with EMG recorded during resting periods. If confirmed by further research, our findings suggesting an involvement of anterior neck muscles during isometric contraction of the jaw elevator muscles may have clinical implications. [ABSTRACT FROM AUTHOR]

Melatonin, the pineal gland hormone known for its ability to modulate circadian rhythm, has recently been studied in its several functions. It is believed to inhibit cancer growth, to stimulate the immune system and to act as an antioxidant. In particular, this latter activity is ascribed to two different mechanisms: stimulation of radical detoxifying enzymes and scavenging of free radicals. We used this compound in mammalian cells in vitro to investigate its mechanism of action in modulating DNA damage. Cytogenetic and cytofluorimetric analyses were performed. We show that melatonin is able to modulate chromosome damage (chromosomal aberrations and sister chromatid exchanges) induced by cyclophosphamide. Conversely, its involvment in modulating oxidative processes, thereby reducing DNA damage, is less clear. In particular, melatonin is able to decrease H2O2-induced chromosomal aberrations but not sister chromatid exchanges and has been found to induce oxygen species in a cytofluorimetric test (DCFH assay). [ABSTRACT FROM PUBLISHER]

This paper describes a familial case of Larsen syndrome. Typical anomalies were present in the propositus and 2 of his 6 daughters. In addition, all patients had progressive deafness and the 2 daughters had cleft palate. The certain exclusion of any consanguinity between the couple, suggests, in this instance, the dominant mode of transmission of the syndrome. [ABSTRACT FROM PUBLISHER]