1. Hydrogen peroxide priming triggers splicing memory in grape berries.
- Author
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Zuo, Ding-Ding, Sun, Hao-Ting, Yang, Lu, Zheng, Meng-Ling, Zhang, Jing, and Guo, Da-Long
- Abstract
Plants are highly sensitive to environmental changes, and alternative splicing (AS) has been described in many studies due to its important control role in stress response. Recent studies indicated that plants exhibit splicing memory to stress to effectively activate transcriptional adaptation. Hydrogen peroxide (H
2 O2 ), as a reactive oxygen species (ROS), has toxic effects on plants. However, it also has a significant effect on promoting early maturity of ‘Kyoho’ grape at low concentrations. To explore the mechanism of priming treatment of H2 O2 showing better promotion effect, the RNA-Seq data of H2 O2 -primied and no-primied fruits were analyzed. The genes with H2 O2 stress splicing memory were identified, accompanied by changes in H3K4me3 modification levels, and their splicing memory patterns were verified by PCR and agarose gel electrophoresis. This finding establishes a link between alternative splicing memory and fruit ripening under H2 O2 regulation and contribute to develop the application of H2 O2 in fruit ripening.Key message: We established a hydrogen peroxide stress-induced platform platform. The changes of alternative splicing and expression patterns mediated by hydrogen peroxide priming treatment were analyzed. Two genes with alternative splicing memory were identified by multi-omics analysis. [ABSTRACT FROM AUTHOR]- Published
- 2024
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