1. Modulation-frequency encoded multi-color fluorescent DNA analysis in an optofluidic chip.
- Author
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Chaitanya Dongre, Jasper van Weerd, Geert A. J. Besselink, Rebeca Martinez Vazquez, Roberto Osellame, Giulio Cerullo, Rob van Weeghel, Hans H. van den Vlekkert, Hugo J. W. M. Hoekstra, and Markus Pollnau
- Subjects
ELECTRONIC modulation ,FLUORESCENCE ,DNA ,OPTOFLUIDICS ,INTEGRATED circuits ,ELECTROPHORESIS ,FOURIER analysis ,PHOTOMULTIPLIERS ,ELECTRONIC excitation - Abstract
We introduce a principle of parallel optical processing to an optofluidic lab-on-a-chip. During electrophoretic separation, the ultra-low limit of detection achieved with our set-up allows us to record fluorescence from covalently end-labeled DNA molecules. Different sets of exclusively color-labeled DNA fragments—otherwise rendered indistinguishable by spatio-temporal coincidence—are traced back to their origin by modulation-frequency-encoded multi-wavelength laser excitation, fluorescence detection with a single ultrasensitive, albeit color-blind photomultiplier, and Fourier analysis decoding. As a proof of principle, fragments obtained by multiplex ligation-dependent probe amplification from independent human genomic segments, associated with genetic predispositions to breast cancer and anemia, are simultaneously analyzed. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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