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Epicuticular waxes have vital roles in the growth and development of plants and in defense. Conifers have a considerable amount of waxes on their cones and leaves. Here we characterized the n-alkane composition of Iranian conifers, including Juniperus oblonga, J. foetidissima, J. sabina, J. communis subsp. hemisphaerica, J. excelsa, Cupressus sempervirens, Platycladus orientalis from Cupressaceae and Taxus baccata from Taxaceae for the first time using GC-FID analyses. In the waxes, 25 n-alkane homologs with chain lengths ranging from C7 to C32 were identified. Short-chain n-alkanes were dominant in almost all samples with some exceptions. Complementary studies to elucidate complete wax constituents of Iranian conifers and n-alkane distribution pattern as a function of geographical and bioclimatic variables are recommended. [ABSTRACT FROM AUTHOR]