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Purpose: This study aimed to explore the differences between patient and nurse perceptions of nursing needs and performance in integrated nursing care service wards within small to medium-sized hospitals. Methods: The participants of the study were 116 patients and 116 nurses in the integrated nursing care service wards of small and medium-sized hospitals in U and Y cities. Data collection was conducted between July 18 and August 12, 2022. The collected data were analyzed using descriptive statistics, the paired t-test, the independent t-test, and the importance-performance analysis. Results: Only the difference between nurse-perceived nursing needs and nursing performance (gap 2) was statistically significant (t=4.18, p<.001). Patients' physical needs were higher than what nurses perceived, and patients' financial were higher than nursing performance as perceived by both nurses and patients. In addition, nurses' performance was lower than the perceived nursing needs in all domains. According to the importance-performance analysis, the "concentrate here" areas included infection control, respect for patients' rights, and education of patients and families about diseases and treatment plans. Additionally, financial issues in nursing were identified as a "low priority" area requiring long-term intervention. Conclusion: It is crucial to prioritize nursing care that addresses patients' needs for infection control, respect for rights, and education. Nurses should also meet the physical and economic support needs of patients. Furthermore, it is recommended to adjust the nurse staffing ratio and foster systematic collaboration among hospitals, taking into account the circumstances of small to medium-sized hospitals. [ABSTRACT FROM AUTHOR]

Human mesenchymal stem cells (MSC), that have been reported to be present in bone marrow, adipose tissues, dermis, muscles and peripheral blood, have the potential to differentiate along different lineages including those forming bone, cartilage, fat, muscle and neuron. This differentiation potential makes MSC excellent candidates for cell-based tissue engineering. In this study, we have examined phenotypes and gene expression profile of the human adipose tissue-derived stromal cells (ATSC) in the undifferentiated states, and compared with that of bone marrow stromal cells (BMSC). ATSC were enzymatically released from adipose tissues from adult human donors and were expanded in monolayer with serial passages at confluence. BMSC were harvested from the metaphysis of adult human femur. Flowcytometric analysis showed that ATSC have a marker expression that is similar to that of BMSC. ATSC expressed CD29, CD44, CD90, CD105 and were absent for HLA-DR and c-kit expression. Under appropriate culture conditions, MSC were induced to differentiate to the osteoblast, adipocyte, and chondrogenic lineages. ATSC were superior to BMSC in respect to maintenance of proliferating ability, and microarray analysis of gene expression revealed differentially expressed genes between ATSC and BMSC. The proliferating ability and differentiation potential of ATSC were variable according to the culture condition. The similarities of the phenotypes and the gene expression profiles between ATSC and BMSC could have broad implications for human tissue engineering. [ABSTRACT FROM AUTHOR]