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Introduction: The rapid spread of COVID-19 worldwide within 2 months demonstrated the vulnerability of the world's population to infectious diseases. In 2015, the Global Antimicrobial Resistance and Use Surveillance System (GLASS) was launched to combat antimicrobial resistance (AMR). However, there has been no comprehensive assessment of the decade-long global battle against AMR based on GLASS data. Methods: South Korea established Kor-GLASS (Korean-GLASS) to proactively monitor data quality and enable international collaborations. A unique feature of Kor-GLASS is the quality control center (QCC), which uses network hubs and ensures standardized, high-quality data through interlaboratory proficiency testing (IPT) and external quality assessment (EQA). In addition, the QCC multifaceted endeavors for integrated data quality management. Results: Since 2020, high-quality AMR data have indicated fluctuating antibiotic resistance rates in South Korea. This trend does not align with the decrease in antibiotic usage seen in humans but coincides with non-human antibiotic sales, indicating a need for greater monitoring of non-human antibiotic resistance. Comprehensive and robust management taking account of the intricate interplay among humans, animals, and the environment is essential. Kor-GLASS has been expanded into a "One Health" multiagency collaborative initiative. Discussion: Although a standardized solution is not suitable for all countries, it must alignwith the local context and international standards. A centralized top-down management structure such as that of the QCC is essential to ensure continuous data quality coordination. Sustained efforts and surveillance systems are crucial for monitoring and managing AMR and safeguarding human health. [ABSTRACT FROM AUTHOR]

Tension wood is a specialized xylem tissue associated with gravitropism in angiosperm trees. However, few regulators of tension wood formation have been identified. The molecular mechanisms underpinning tension wood formation remain elusive. Here, we report that a Populus KNOTTED-like homeobox gene, PagKNAT2/6b , is involved in tension wood formation and gravity response. Transgenic poplar plants overexpressing PagKNAT2/6b displayed more sensitive gravitropism than controls, as indicated by increased stem curvature. Microscopic examination revealed greater abundance of fibre cells with a gelatinous cell wall layer (G-layer) and asymmetric growth of secondary xylem in PagKNAT2/6b overexpression lines. Conversely, PagKNAT2/6b dominant repression plants exhibited decreased tension wood formation and reduced response to gravity stimulation. Moreover, sensitivity to gravity stimulation showed a negative relationship with development stage. Expression of genes related to growth and senescence was affected in PagKNAT2/6b transgenic plants. More importantly, transcription activation and electrophoretic mobility shift assays suggested that PagKNAT2/6b promotes the expression of cytokinin metabolism genes. Consistently, cytokinin content was increased in PagKNAT2/6b overexpression plants. Therefore, PagKNAT2/6b is involved in gravitropism and tension wood formation, likely via modulation of cytokinin metabolism. [ABSTRACT FROM AUTHOR]

Background: Tuberculosis (TB) remains a significant public health concern globally, particularly in regions with a high prevalence of HIV/AIDS. In Tanzania, the co-occurrence of TB and HIV poses a substantial challenge to healthcare systems. Isoniazid Preventive Therapy (IPT) has become an important intervention to mitigate the risk of TB infection among individuals living with HIV. Despite its proven efficacy, the extent of knowledge, attitude, and practice of healthcare workers (HCWs) in Tanzania is not well-documented. This study aimed to evaluate the levels of knowledge, attitude, and practice regarding IPT among healthcare workers. Methods: A cross-sectional study was carried out among healthcare workers working at TB/HIV clinics in 12 regions of Tanzania mainland. A semi-structured questionnaire was administered to the respondents consisting of social demographic characteristics, knowledge, attitude, and practice questions. Data were analyzed using Stata version 14.0. Results: A total of 464 respondents were interviewed, with 282 (60.8%) being female. The mean age was 36.4 years (SD = 11.0). Among the interviewed, 255 (55.0%) were nurses. Regarding knowledge, attitude, and practice on IPT, 217 (46.8%), 220 (47.0%), and 422 (90.9%) respondents respectively demonstrated adequacy in these domains. Factors such as gender, university education, professional role in healthcare practice, cumulative work experience of one year or more, tenure at a TB/HIV clinic of one year or longer, and attendance at IPT-related training emerged as significant determinants. These factors influence respondents' knowledge, attitude, and practice towards IPT. Conclusion: The findings underscored a notable knowledge gap and a less favourable attitude towards IPT among HCWs, despite commendable practices. Addressing this disparity through targeted training interventions for healthcare workers holds promise for enhancing their understanding and attitude toward IPT, thereby fostering increased acceptance and utilization of this preventive therapy. [ABSTRACT FROM AUTHOR]

Purpose: The volume of arthroscopic hip surgery has increased dramatically in recent years with iliopsoas tendinitis (IPT) being one of the most common complications of this procedure. The purpose of this study is to investigate the prevalence of post-operative IPT in patients who undergo arthroscopic hip surgery with capsular closure using absorbable versus non-absorbable suture. Methods: This is a single center, single surgeon, retrospective analysis performed between August 2007 and May 2023 comparing two cohorts who underwent hip arthroscopy. Patients were divided into those who underwent surgery with capsular closure using absorbable (Vicryl®, Johnson and Johnson, New Brunswick, NJ) suture and those who underwent capsular closure with non-absorbable suture (Suturetape, Arthrex, Naples FL). All patients who underwent primary or revision hip arthroscopy and were at least 2 months post-operation were initially included in the study. Results: Between August 2007 and May 2023 a total of 1513 hip arthroscopy surgeries were performed. Within this cohort, 1421 hips underwent hip arthroscopy with non-absorbable suture and 64 hips underwent surgery with absorbable suture. There was no significant difference between the proportion of IPT in the non-absorbable cohort (2.3%) versus the absorbable cohort (1.6%) (P = 0.669). Conclusion: Capsular closure with Absorbable sutures was non-inferior to capsular closure with non-absorbable sutures with respect to the proportion of post-operative IPT following hip arthroscopy for FAI. Additionally, the proportion of post-operative IPT was found to be significantly higher in patients undergoing revision versus primary hip arthroscopy, regardless of capsular closure suture type. Level of evidence: IV. [ABSTRACT FROM AUTHOR]

Over the last decade, remarkable progress has been made in our understanding the phytohormones, cytokinin's (CKs) biosynthesis, perception, and signalling pathways. Additionally, it became apparent that interfering with any of these steps has a significant effect on all stages of plant growth and development. As a result of their complex regulatory and cross-talk interactions with other hormones and signalling networks, they influence and control a wide range of biological activities, from cellular to organismal levels. In agriculture, CKs are extensively used for yield improvement and management because of their wide-ranging effects on plant growth, development and physiology. One of the primary targets in this regard is cytokinin oxidase/dehydrogenase (CKO/CKX), which is encoded by CKX gene, which catalyses the irreversible degradation of cytokinin. The previous studies on various agronomically important crops indicated that plant breeders have targeted CKX directly. In recent years, prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been increasingly used in editing the CKO/CKX gene and phenomenal results have been achieved. This review provides an updated information on the applications of CRISPR-based gene-editing tools in manipulating cytokinin metabolism at the genetic level for yield improvement. Furthermore, we summarized the current developments of RNPmediated DNA/transgene-free genomic editing of plants which would broaden the application of this technology. The current review will advance our understanding of cytokinins and their role in sustainably increase crop production through CRISPR/Cas genome editing tool. [ABSTRACT FROM AUTHOR]

Background: Little is known about isoniazid preventive therapy (IPT) completion rates among children or adolescents compared to adults living with HIV in Kinshasa, Democratic Republic of the Congo (DRC). Methods: We conducted a retrospective cohort analysis including children, adolescents, and adults living with HIV who were treated at FHI360 and partners‐implemented HIV care programs at six health zones in Kinshasa, DRC, from 2004 to 2020. The primary outcome was the proportion of children, adolescents versus adults who did complete 6 months of daily self‐administered IPT. Log‐binomial regression assessed independent predictors of IPT non‐completion and Kaplan–Meier technique for survival analysis. Results: Of 11,691 eligible patients on ART who initiated IPT, 429 were children (<11 years), 804 adolescents (11–19 years), and 10,458 adults (≥20 years). The median age was 7 (IQR: 3–9) years for children, 15 (IQR: 13–17) years for adolescents, and 43 (35–51) years for adults. Among those who were initiated on IPT, 5625 out of 11,691 people living with HIV (PLHIV) had IPT completion outcome results, and an overall 3457/5625 (61.5%) completion rate was documented. Compared to adults, children and adolescents were less likely to complete IPT [104/199 (52.3%) and 268/525 (51.0%), respectively, vs. 3085/4901 (62.9%)]. After adjustment, the only independent predictors for IPT non‐completion were health zone of residence and type of ART regimen. Kaplan–Meier analysis showed comparable poor survival among patients who completed IPT versus those who did not (p‐value for log‐rank test, 0.15). Conclusions: The overall sub‐optimal IPT completion rate in adults as well as children/adolescents in this setting is of great concern. Prospective studies are needed to elucidate the specific barriers to IPT completion among children, adolescents, and adults in DRC as well as the scale‐up of evidence‐informed interventions to improve IPT completion, such as adoption of shorter TB preventive regimens. [ABSTRACT FROM AUTHOR]

Background: Approximately 32 million pregnant women are at risk of malaria with up to 10,000 maternal deaths and 200,000 neonates at risk annually. Intermittent Preventive Treatment (IPT) with sulfadoxine-pyrimethamine (SP) is recommended by the World Health Organization (WHO) to reduce disease in pregnancy and adverse maternal and newborn outcomes. At least three doses of SP should be taken by pregnant women during antenatal consultation (ANC) beginning from the thirteenth week of pregnancy till parturition. The aim of this study was to assess uptake of IPT during pregnancy and risk factors for maternal anaemia and infant birth weight in Dschang, West region of Cameroon. Methods: A total of 380 consenting pregnant women at delivery were recruited in a cross- sectional prospective survey between January to December 2021. Data on ANC attendance, total dose of IPT and history of malaria were abstracted from hospital ANC records while socio-demographic characteristics, bed net use and obstetrics history of each participant were also recorded through an interview. Further, blood samples were collected from the intervillous space for assessment of maternal anaemia and microscopic parasitology. Nested PCR based on amplification of the Plasmodium 18S sRNA was carried out to detect submicroscopic infection. IPTp coverage was calculated per WHO recommendation and the prevalence of anaemia and low birth weight were estimated as proportions in the total sample of pregnant women and live births, respectively. Crude and adjusted odds ratios and their 95% confidence intervals were used to estimate associations between pregnancy outcomes considered and risk factors in specific and general models. A p < 0.05 was considered significant. The R software (V4.1.4) was used for all analyses. Results: A majority of pregnant women was aged between 24 and 34 years old (59.2%) and had secondary education (58.8%). Uptake of ≥ 3 IPTp was 64.99% with 77.20% of all who received at least one IPTp doses taking a mix of SP and DP or DP alone in successive ANC contacts. Those with four or more ANC contacts (73.42%) were more likely to have received at least one IPTp. Furthermore, 13.9% of live births had low birthweights (BW < 2500 g) and one in four parturient women with moderate anaemia by WHO criteria. Microscopy (blood smear examination) and PCR-based diagnosis revealed between 0% and 1.57% of parasite-infected placental samples, respectively. Reported malaria in pregnancy predicted maternal anaemia at birth but not birth weight. Only gestational age (< 37 weeks) and bed net use (< 5 months) significantly predicted infant birth weight at delivery. Conclusion: The uptake of WHO recommended IPT doses during pregnancy was moderately high. Reported malaria in pregnancy, poor bed net coverage, gestational age less than 37 weeks adversely affect maternal haemoglobin levels at birth and infant birth weight. Asymptomatic and submicroscopic placental parasite infections was found at low prevalence. Together these results highlight the importance of maintaining aggressive measures to prevent malaria in pregnancy and protect the health of mother and baby. [ABSTRACT FROM AUTHOR]

Social dysfunction plays a critical role in the development and maintenance of depression in both adolescents and adults. Interpersonal psychotherapy (IPT) and interpersonal psychotherapy for depressed adolescents (IPT-A) are effective, evidence-based, and time-limited treatments for depression that aim to mitigate depressive symptoms by strengthening an individual's interpersonal relationships and skills. Though the efficacy of IPT/IPT-A has been well established, we are just beginning to know how biological systems are implicated in its success. In this scoping review, we examine the extant literature on biological mechanisms and predictors of IPT/IPT-A treatment efficacy. Overall, seven studies were identified that consider biological processes in the context of evaluating IPT/IPTA, and the studies that were conducted are typically preliminary in nature. Notably, there is some evidence showing that the hypothalamic–pituitary–adrenal axis, various frontal and limbic brain regions, and behavioral indexes that represent brain functioning are associated with changes in IPT/IPT-A or predictive of IPT/IPT-A outcomes. We also consider consequences for treatment and future research. The hope is that a better understanding of how and for whom IPT/IPT-A works can optimize the success of the treatment in reducing an individual's depressive symptoms. [ABSTRACT FROM AUTHOR]

This paper presents a solution to a limitation of wireless power transfer that arises when using two D-shaped transmitter and receiver coils. Double D, or DD, coils are well known to have a polar, directional magnetic field, which increases the misalignment tolerance in one of the directions. The misalignment tolerance is nonsymmetric, and it is significantly better in one of the directions, which can also be considered a shortcoming. An additional shortcoming of the DD coil is that it is dependent on the rotation around the z-axis, due to the directional magnetic field. This is not a problem when using classic planar spiral coils, which do not generate a directional magnetic field. Therefore, DD coils are not suitable for applications in which the z-axis orientation is not determined and fixed to specific angle and direction. This paper presents a unique design of a transmitter coil, based on a double DD coil. The transmitter coil consists of two DD coils which are perpendicular to each other. The proposed transmitter structure can excite the receiver DD coil in a way that the efficiency of the power transfer is the highest, regardless of the orientation. The proposed transmitter structure can, therefore, solve the problem with rotation of a single DD coil. The proposed system structure was tested on the small-scale experimental setup. [ABSTRACT FROM AUTHOR]

Early in the history of cytokinins, it was clear that Zea mays seeds contained not just trans-zeatin, but its nucleosides and nucleotides. Subsequently, both pods and seeds of legumes and cereal grains have been shown to contain a complex of cytokinin forms. Relative to the very high quantities of cytokinin detected in developing seeds, only a limited amount appears to have been translocated from the parent plant. Translocation experiments, and the detection of high levels of endogenous cytokinin in the maternal seed coat tissues of legumes, indicates that cytokinin does not readily cross the maternal/filial boundary, indicating that the filial tissues are autonomous for cytokinin biosynthesis. Within the seed, trans-zeatin plays a key role in sink establishment and it may also contribute to sink strength. The roles, if any, of the other biologically active forms of cytokinin (cis-zeatin, dihydrozeatin and isopentenyladenine) remain to be elucidated. The recent identification of genes coding for the enzyme that leads to the biosynthesis of trans-zeatin in rice (OsCYP735A3 and 4), and the identification of a gene coding for an enzyme (CPN1) that converts trans-zeatin riboside to trans-zeatin in the apoplast, further cements the key role played by trans-zeatin in plants. [ABSTRACT FROM AUTHOR]

Medical interventions have improved the lives and wellbeing of prematurely born infants and their families,however, mental health interventions among these families have yet to become more widely implemented. Approximately 10 % of the population in the United States is born prematurely, with a higher incidence among families of color. Parents of premature babies face several challenges that negatively influence their mental health, their ability to bond with their babies, and ultimately their children's development. In addition to the biological impairment that certain premature babies face, their experience of medical interventions at such a young age increases their caregiving needs. This paper aims to review the literature about caregivers' attachment representation and sensitivity and its impact on caregivers' behaviors and infant attachment. It also overviews effective early interventions emphasizing the caregiver--infant relationship treatment guided by the principles of Infant and Early Childhood Mental Health due to the reverberating influence of prematurity in the whole family. [ABSTRACT FROM AUTHOR]

Copyright of Ordnance Industry Automation is the property of Editorial Board for Ordnance Industry Automation and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: Interpersonal Psychotherapy (IPT) is an evidence-based therapy. There have been increasing demand and training opportunities of IPT in China. Reviewing current evidence on its use in Chinese patients can help us understand the applicability of IPT in China and identify knowledge gaps to encourage and better future research in this field. Method: We did a comprehensive search of three major electronic databases: PubMed (English), Chinese National Knowledge Infrastructure (CNKI) and WanFang Data (Chinese). We examined overall study design, outcome measures, data analyses and other parameters. We only selected articles of Randomized Clinical Trials (RCT) for this review. All study findings were grouped and summarized per psychiatric diagnoses. The meta-analysis and forest plots were performed whereas studies could be combined. Results: After a full text review of 132 articles, 40 were selected for the final review. Comparing with control groups, evidences supported the efficacy of IPT in Chinese patients with Major Depressive Disorder (MDD), Postpartum depression, Generalized Anxiety Disorder (GAD), Social Anxiety Disorder, Post Stress Traumatic Disorder (PTSD), and Post-psychotic Depression. It was also beneficial to college students and Chinese first-time mothers. Meta-analysis using a random-effects model consistently yielded significant score differences between the IPT and control groups (p < 0.0001) on MDD. Conclusion: This systematic review has identified the current best evidence for IPT efficacy in Chinese population. The findings support IPT as an effective treatment in Chinese with certain psychiatric conditions, consistent with those from many other studies throughout the world. [ABSTRACT FROM AUTHOR]

Wireless power transmission (WPT) is popular and gaining technology finding its application in various fields. The power is transferred from a source to an electrical load without the need of interconnections. Wireless power transmission (WPT) is useful to power electrical devices where physical wiring is not possible or inconvenient. The technology uses the principle of mutual inductance. One of the future applications finds in automotive sector especially in Electric Vehicles. This project deals with research and development of wireless charging systems for Electric vehicles using wireless transmission. The main goal is to transmit power using resonance coupling and to build the charging systems. The systems deal with an AC source, transmission coil, reception coil, converter and electric load which are battery. [ABSTRACT FROM AUTHOR]

In this paper, an inductive power transfer (IPT) system without compensation elements is presented for small house appliances. The proposed system's transmitter side is an independent induction heating cooktop. IPT can be achieved when the kettle with the receiving coils is placed on the transmitter coil. The coils are designed with a high coupling coefficient. The magnetic system model consisting of aligned transmitter and receiver coils is created in the Maxwell program. In the created model, the analysis depends on the air gap and frequency, which are the variables that affect the wireless power transfer. The electronic circuit simulation uses the coil model to examine the system's dynamic behavior. The design of the transmitter/receiver coils of the IPT system is made with a cylindrical coil with a diameter of 145 mm, taking into account that it is compatible with the dimensions of the existing kettle and induction heating cooktops coil. A half-bridge series resonant converter circuit is used to adjust the power transferred to the load. To verify the simulation results and test the designed system, an experimental circuit using a 2200 W kettle is carried out. In the experiments, the air gap between the coils is kept constant at 7 mm, and measurements are taken for different powers. Experimental results confirm the magnetic model and simulation results. As a result, wireless power transfer is realized in a wide range without loss of performance in the kettle. System efficiency is greater than the 90% specified in the Ki cordless kitchen standard, and the harmonic currents drawn from the mains are lower than the values determined by the IEC 61000-3-2 standard. [ABSTRACT FROM AUTHOR]

High misalignment-insensitive capacity is of great importance for inductive power transfer (IPT) systems. A hybrid topology based on a parameter optimization design method is presented to obtain constant current output regardless of the coupling and load. DDQ coils are employed to protect against system key parameter changes, and a particle swarm optimization (PSO) method has been presented to achieve a nearly constant current output without using complex control schemes. A hybrid IPT system using DDQ coils has been built. The experimental results verify that the current fluctuation is within 5% when the load varies from 5 Ω to 10 Ω within 50% misalignment of the coupling pads. Moreover, the maximum system DC–DC efficiency is up to 91%. [ABSTRACT FROM AUTHOR]

This paper describes the control of a wireless power transfer system using a double DD coil structure, when the transmitter and the receiver coil are rotated to one another. WPT systems using single DD coils are rotationally dependent, due to the directional magnetic field generated by the DD transmitter coil. This rotational dependance can be mitigated by using a different transmitter structure. One such possibility is the usage of the double DD coil on the transmitter and the receiver sides. A double DD coil includes two directional DD coils, which can be excited separately. The coils inside the coil structure are perpendicular to one another, which allows higher power density and additional rotation tolerance. The proposed system was tested on a low power laboratory experimental setup. [ABSTRACT FROM AUTHOR]

Individuals living with Parkinson's disease (PD) experience interpersonal stressors that contribute to depressive risk. Interpersonal psychotherapy (IPT) emphasizes the bidirectional relationship between interpersonal stressors and mood may therefore be a suitable treatment for PD-depression. The primary aim of this study was to evaluate the feasibility of delivering 12 sessions of IPT to depressed PD patients and explore the need for modifications. A secondary aim was to obtain descriptive information about efficacy outcomes. The study used a pre-post design without a comparison group. Participants were 12 PD patients with a major depressive disorder. IPT was well accepted and tolerated by patients and required minimal modifications. Compliance with session attendance and completion of study questionnaires were excellent and treatment satisfaction was high. Depression scores declined from baseline to endpoint, with 7 patients meeting criteria for remission at endpoint. Findings are encouraging and a larger randomized controlled trial is currently underway to ascertain if IPT is an efficacious treatment for PD-depression. [ABSTRACT FROM AUTHOR]

Accessibility in the education system for the Disabled (OKU) at the tertiary level has become the talk and attention at the university level to raise awareness of this group. Awareness of the disabilities of this group is very important in fighting for their rights and giving them equal opportunities. This study is a necessary problem in academics in creating awareness of the needs of the disabled in Institutions of Higher Learning (IPT). This study uses a qualitative research method of in-depth interviews with eleven (11) respondents consisting of six (6) academic students who teach students with disabilities and five (5) students with disabilities using the categories of visual impairment, hearing and other categories. Competency study in two IPTs at the Selangor International Islamic University College (KUIS) and the Sultan Salahuddin Abdul Aziz Shah Polytechnic. The findings obtained from the search for academic students in IPT are still at a low level. This is because there is still a lack of knowledge and needs required when dealing with students with disabilities in IPT. So in this study, the researcher will unravel the challenges and energy of teachers in realizing special education at the IPT level. This is because of the increasing number of disabilities, therefore, it is very important for more intervention programs and comprehensive discussions supported to increase awareness of relationships in the community. [ABSTRACT FROM AUTHOR]

In Arabidopsis thaliana, the histone acetyltransferase GCN5 and the associated coactivator ADA2b regulate root growth and affect gene expression. The cytokinin signaling reporter TCS::GFP was introduced into gcn5-1, ada2b-1, and ada2a-2, as well as the ada2a-2ada2b-1 mutants. The early root growth (4 to 7 days post-germination) was analyzed using cellular and molecular approaches. TCS signal accumulated from the fourth to seventh days of root growth in the wild-type columella cells. In contrast, ada2b-1 and gcn5-1 and ada2a-2ada2b-1 double mutants displayed reduced TCS expression relative to wild type. Gene expression analysis showed that genes associated with cytokinin homeostasis were downregulated in the roots of gcn5-1 and ada2b-1 mutants compared to wild-type plants. H3K14 acetylation was affected in the promoters of cytokinin synthesis and catabolism genes during root growth of Arabidopsis. Therefore, GCN5 and ADA2b are positive regulators of cytokinin signaling during root growth by modulating histone acetylation and the expression of genes involved in cytokinin synthesis and catabolism. Auxin application in the roots of wild-type seedlings increased TCS::GFP expression. In contrast, ada2b and ada2ada2b mutant plants do not show the auxin-induced TCS signal, suggesting that GCN5 and ADA2b are required for the auxin-induced cytokinin signaling in early root growth. [ABSTRACT FROM AUTHOR]

Over the last decade, remarkable progress has been made in our understanding the phytohormones, cytokinin's (CKs) biosynthesis, perception, and signalling pathways. Additionally, it became apparent that interfering with any of these steps has a significant effect on all stages of plant growth and development. As a result of their complex regulatory and cross-talk interactions with other hormones and signalling networks, they influence and control a wide range of biological activities, from cellular to organismal levels. In agriculture, CKs are extensively used for yield improvement and management because of their wide-ranging effects on plant growth, development and physiology. One of the primary targets in this regard is cytokinin oxidase/dehydrogenase (CKO/CKX), which is encoded by CKX gene, which catalyses the irreversible degradation of cytokinin. The previous studies on various agronomically important crops indicated that plant breeders have targeted CKX directly. In recent years, prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been increasingly used in editing the CKO/CKX gene and phenomenal results have been achieved. This review provides an updated information on the applications of CRISPR-based gene-editing tools in manipulating cytokinin metabolism at the genetic level for yield improvement. Furthermore, we summarized the current developments of RNP-mediated DNA/transgene-free genomic editing of plants which would broaden the application of this technology. The current review will advance our understanding of cytokinins and their role in sustainably increase crop production through CRISPR/Cas genome editing tool. [ABSTRACT FROM AUTHOR]

Background: Despite evidence that isoniazid preventive therapy (IPT) can reduce the risk of tuberculosis (TB) disease among People Living with Human Immunodeficiency Virus (PLHIV), uptake of IPT is low in many resource-limited settings. This study determined the level of IPT uptake and its associated factors amongst PLHIV. Materials and Methods· This was a retrospective quantitative study amongst PLHIV who do not have active TB and enrolled in 2019 - 2020 for anti-retroviral therapy (ART) in Butebo district, Uganda. Data related to demographic factors (age, sex, religion, marital status, employment status, education level, area of residence, household density), health facility factors (pre-1PT counseling), community factors [distance from Health Center (H/C), incurred costs to reach H/C], and IPT drug-related factors [frequency of lsoniazid (INH) refill, INH stock-outs] were collected from four health facilities using a checklist. The data was analyzed into descriptive statistics and relationships determined using Chi-square tests. Results: Among eligible PLHIV (272), 34.2% achieved IPT uptake. The mean duration between HIV diagnosis and the start of IPT was 4.31 years, with IPT Uptake among males (37.0%), females (32.8%), married (39.5%), and Christians (35.4%). Factors that affect the rate of IPT uptake include employment, education, residence, costs to reach H/C, and pre IPT counseling. The IPT completion rate was 97.80/o. All the cases who had regular INH refill completed IPT compared to 60.0°/0 with the irregular refill, while 97.8% did not experience INH stock-outs and completed IPT. Conclusion: Pre-IPT counseling was the most significant contributing factor for IPT uptake.1PT uptake may be scaled up by integrating IPT services in routine HIV care, enhancing counseling for IPT and supervision and monitoring, training of health workers, and improving logistical supplies at the health centers. [ABSTRACT FROM AUTHOR]

Aim of the study: Inflammatory pseudotumor is a rare benign tumor that can occur at various body sites. Due to its rare occurrence and histological variety radiological data is heterogeneous and limited. Case description: We present a case of a 71-year-old man with inflammatory pseudotumor of the omentum. Contrast-enhanced ultrasound perfusion pattern showed homogeneous, isoechoic enhancement in the arterial phase with a washout phenomenon in the parenchymal phase, mimicking a peritoneal carcinomatosis. Conclusions: Inflammatory pseudotumor represents a rare, but important benign differential diagnostic option when considering a malignant disorder. Contrast-enhanced ultrasound is helpful in identifying vital tissue for a targeted biopsy for subsequent histological examination that is essential for the exclusion of malignancy. [ABSTRACT FROM AUTHOR]

Copyright of Plural - Revista de Ciências Sociais is the property of Revista Plural and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Summary: Cytokinins play a central role in delaying senescence, reducing oxidative damage and maintaining plant growth during drought. This study showed that the ectopic expression of ProRE‐deleted MdIPT5b, a key enzyme involved in cytokinin metabolism, increased the drought tolerance of transgenic Malus domestica (apple) callus and Solanum lycopersicum (tomato) seedlings by maintaining cytokinin homeostasis, and thus maintaining redox balance. Under restricted watering regimes, the yields of transgenic tomato plants were enhanced. Heterodimers of C/S1 bZIP are involved in the cytokinin‐mediated drought response. The heterodimers bind the ProRE of MdIPT5b promoter, thus directly suppressing gene transcription. Single C/S1 bZIP members could not independently function as suppressors. However, specific paired members (heterodimers of MdbZIP80 with MdbZIP2 or with MdbZIP39) effectively suppressed transcription. The α‐helical structure is essential for the heterodimerization of C/S1 bZIP members and for synergistic transcriptional suppression. As negative regulators of drought tolerance, suppressing either MdbZIP2 or MdbZIP39 alone does not improve the expression of MdIPT5b and did not increase the drought tolerance of transgenic apple callus. However, this could be achieved when they were co‐suppressed. The suppression of MdbZIP80 alone could improve MdIPT5b expression and increase the drought tolerance of transgenic apple callus. However, these effects were reversed in response to the cosuppression of MdbZIP80 and MdIPT5b. Similar results were also observed during delayed dark‐induced senescence in apple leaves. In conclusion, the apple C/S1 bZIP network (involving MdbZIP2, MdbZIP39 and MdbZIP80) directly suppressed the expression of MdIPT5b, thus negatively modulating drought tolerance and dark‐induced senescence in a functionally redundant manner. Significance Statement: This study proposes the condition of cytokinin homeostasis (appropriate and steady concentration), which counteracts leaf senescence and essentially improves drought tolerance. A regulatory relationship was found between the C/S1 bZIP network and cytokinins, which provides insights into cytokinin regulation under drought stress. [ABSTRACT FROM AUTHOR]

Summary: Isopentenyltransferase (IPT) in plants regulates a rate‐limiting step of cytokinin (CTK) biosynthesis. IPTs are recognized as key regulators of CTK homeostasis and phytohormone crosstalk in both biotic and abiotic stress responses. Recent research has revealed the regulatory function of IPTs in gene expression and metabolite profiles including source‐sink modifications, energy metabolism, nutrient allocation and storage, stress defence and signalling pathways, protein synthesis and transport, and membrane transport. This suggests that IPTs play a crucial role in plant growth and adaptation. In planta studies of IPT‐driven modifications indicate that, at a physiological level, IPTs improve stay‐green characteristics, delay senescence, reduce stress‐induced oxidative damage and protect photosynthetic machinery. Subsequently, these improvements often manifest as enhanced or stabilized crop yields and this is especially apparent under environmental stress. These mechanisms merit consideration of the IPTs as 'master regulators' of core cellular metabolic pathways, thus adjusting plant homeostasis/adaptive responses to altered environmental stresses, to maximize yield potential. If their expression can be adequately controlled, both spatially and temporally, IPTs can be a key driver for seed yield. In this review, we give a comprehensive overview of recent findings on how IPTs influence plant stress physiology and yield, and we highlight areas for future research. [ABSTRACT FROM AUTHOR]

Background International Maternal Pediatric Adolescent AIDS Clinical Trials (IMPAACT) P1078, a randomized noninferiority study designed to compare the safety of starting isoniazid preventive therapy (IPT) in women living with human immunodeficiency virus (HIV) either during pregnancy or after delivery, showed that IPT during pregnancy increased the risk of composite adverse pregnancy outcomes, but not individual outcomes. Many known factors are associated with adverse pregnancy outcomes: these factors' associations and effect modifications with IPT and pregnancy outcomes were examined. Methods Pregnant women living with HIV from 8 countries with tuberculosis incidences >60/100 000 were randomly assigned to initiate 28 weeks of IPT either during pregnancy or at 12 weeks after delivery. Using univariable and multivariable logistic regression and adjusting for factors associated with pregnancy outcomes, composite and individual adverse pregnancy outcome measures were analyzed. Results This secondary analysis included 925 mother-infant pairs. All mothers were receiving antiretrovirals. The adjusted odds of fetal demise, preterm delivery (PTD), low birth weight (LBW), or a congenital anomaly (composite outcome 1) were 1.63 times higher among women on immediate compared to deferred IPT (95% confidence interval [CI], 1.15–2.31). The odds of fetal demise, PTD, LBW, or neonatal death within 28 days (composite outcome 2) were 1.62 times higher among women on immediate IPT (95% CI, 1.14–2.30). The odds of early neonatal death within 7 days, fetal demise, PTD, or LBW (composite outcome 3) were 1.74 times higher among women on immediate IPT (95% CI, 1.22–2.49). Conclusions We confirmed higher risks of adverse pregnancy outcomes associated with the initiation of IPT during pregnancy, after adjusting for known risk factors for adverse pregnancy outcomes. [ABSTRACT FROM AUTHOR]

Objective: Interpersonal Psychotherapy (IPT) model, with its four problematic areas of grief, deficit, role transition and role dispute, provides a useful frame of reference for a quick case formulation. We aimed at applying the IPT problematic areas assessment in a sample of patients from a liaison psychiatry setting. Methods: One-hundred and twenty-nine hospitalized patients of both sexes, aged between 18 and 80 years were interviewed. The 'Interpersonal Problem Areas Rating Scale' (IPARS) was used to detect the interpersonal focuses. Results: IPARS problematic areas were identified in the 76% of the sample (n=98). Grief and role transition, interpersonal deficits and role disputes were, respectively, the most frequently (43.4 and 42.6%, respectively) and the less frequently described focuses (14 and 11.6%). Moreover, 31 patients (24%) showed no problem areas related to current symptomatology. Conclusions: The IPT model has proved to be an easy-to-use tool, able to guide the psychological interview and allowing the collection of information from an interpersonal perspective in a short time, although no specific focuses were detected as related to current psychological distress in around 25% of the sample. [ABSTRACT FROM AUTHOR]

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The success rate of endodontic treatment is lower than expected, and is both time-consuming and expensive. Thus, techniques to enable a clinician to avoid it deserve more attention. Indirect pulp treatment (IPT) is a simple and effective treatment to preserve pulp vitality in teeth with deep carious lesions. Scientific data supports this treatment as means to preserving a healthy, vital pulp. [ABSTRACT FROM AUTHOR]

Background: Pediatric tuberculosis (TB), human immunodeficiency virus (HIV), and TB-HIV co-infection are health problems with evidence-based diagnostic and treatment algorithms that can reduce morbidity and mortality. Implementation and operational barriers affect adherence to guidelines in many resource-constrained settings, negatively affecting patient outcomes. This study aimed to assess performance in the pediatric HIV and TB care cascades in Mozambique. Methods: A retrospective analysis of routine PEPFAR site-level HIV and TB data from 2012 to 2016 was performed. Patients 0–14 years of age were included. Descriptive statistics were used to report trends in TB and HIV indicators. Linear regression was done to assess associations of site-level variables with performance in the pediatric TB and HIV care cascades using 2016 data. Results: Routine HIV testing and cotrimoxazole initiation for co-infected children in the TB program were nearly optimal at 99% and 96% in 2016, respectively. Antiretroviral therapy (ART) initiation was lower at 87%, but steadily improved from 2012 to 2016. From the HIV program, TB screening at the last consultation rose steadily over the study period, reaching 82% in 2016. The percentage of newly enrolled children who received either TB treatment or isoniazid preventive treatment (IPT) also steadily improved in all provinces, but in 2016 was only at 42% nationally. Larger volume sites were significantly more likely to complete the pediatric HIV and TB care cascades in 2016 (p value range 0.05 to < 0.001). Conclusions: Mozambique has made significant strides in improving the pediatric care cascades for children with TB and HIV, but there were missed opportunities for TB diagnosis and prevention, with IPT utilization being particularly problematic. Strengthened TB/HIV programming that continues to focus on pediatric ART scale-up while improving delivery of TB preventive therapy, either with IPT or newer rifapentine-based regimens for age-eligible children, is needed. [ABSTRACT FROM AUTHOR]

The label transcription and imaging of specimens in key African herbaria has been ongoing since the early 2000s. Many collections in Benin, Cameroon, Côte d'Ivoire, Gabon, Guinea Conakry, and Togo are now fully transcribed and partially digitized. More than 200 000 transcribed specimens are available with the following distribution: • Benin: 45 000 • Cameroon: 70 000 • Côte d'Ivoire: 18 000 • Gabon: 70 000 • Guinea Conakry: 5 000 • Togo: 15 000 In April 2021, a BID project was started to deliver a regional data platform of West and Central African herbaria. Biodiversity Information for Development (BID) is a multi-year programme funded by the European Union and led by GBIF with the aim of enhancing capacity for effective mobilization and use of biodiversity data in research and policy in the 'ACP' nations of sub-Saharan Africa, the Caribbean and the Pacific. Our project's funding runs from April 2021 to April 2023. At this stage of the project, we are working on defining the information technology (IT) architecture (Fig. 1) and selecting the tools that we will be using to achieve our goals. In the talk, we will present our conclusions through architecture schemas and tools demonstrations. Each of the 6 countries will have its own PostgreSQL database, storing its data. They will also have access to the RIHA data management platform (Réseau Informatique des Herbiers d'Afrique / Digital Network of African Herbaria). This is a web application, developed in PHP, allowing full management of the data by herbarium administrators (Fig. 2). An Integrated Publishing Toolkit (IPT) will fetch these herbaria data from the databases, create the Darwin Core archives, and connect these data automatically to gbif.org on a periodic basis (Fig. 3). On the databases, we will use a PostgreSQL view to ease conversion from the RIHA data model to the Darwin Core model. On the IPT, we will create one dataset per country, linked to each PostgreSQL view. The SQL query will be configured to only fetch validated data, depending on the herbarium administrator's validation in the RIHA platform. The automatic and periodic data transmission to gbif.org is a feature available in the IPT, and recently improved by the GBIF France team, which contributes to the IPT development. Another part of the automatic data workflow will be to feed a Living Atlases portal for the West and Central African herbaria. This web application will allow public users to search, display and download herbaria data from West and Central Africa (Fig. 4). Internally, this Living Atlases application will reuse open source modules developed by the Atlas of Living Australia (ALA). The application is mainly written in Java, uses JQuery/Bootstrap for the interface and relies on SolR and Spark in the backend. It has been developed to be easily reusable, by only modifying configuration and doing web customization (HTML / CSS), hiding most of the backend technological complexity. The automatic data workflow will transfer datasets generated by the IPT, in Darwin Core Archive format, to the Living Atlases portal backend. A technical task orchestrator, yet to be selected, will implement this feature. Living Atlases subportals, limited to data of one participating country, could be easily set up, leveraging the existing backend resources (Fig. 5). One of the benefits of the Living Atlases portal is that we can easily deploy additional front end applications with limited data, configured by a filter (here, a filter on the data owner country). Only configuration and web customization (HTML / CSS) are required. All the backend modules, especially the ones storing data, are shared by the multiple front-ends, limiting the hardware consumption and data administration. The full automation of the workflow will allow this platform to run at a very low maintenance cost for IT administrators. Moreover, adding a new herbarium member from West and Central Africa will be quite easy thanks to the architecture of the Integrated Publishing Toolkit and Living Atlases tools (Fig. 6). [ABSTRACT FROM AUTHOR]

In recent years, suicidal behaviors have shown substantial increase worldwide. This trend is also prominent in Israel and has led to a dramatic increase in mental health treatment demand resulting in long wait times and low treatment acceptance rate. To address the critical need in crisis intervention for children and adolescents at suicidal risk we developed an ultra-brief acute crisis intervention, based on Interpersonal Psychotherapy (IPT). IPT is an evidence-based intervention for various psychopathologies among different age groups. The current adaptation of IPT-A is comprised of five weekly sessions, followed by monthly follow-up caring email contacts to the patients and their parents, over a period of 3 months. This paper aims to review the theoretical foundation of this intervention, describe the research design, and present preliminary results of a pilot study. Preliminary Results from our samples of 26 adolescents indicate meaningful trends for both the suicidal ideation (SIQ) and depression (MFQ) outcome measures. Significant interaction was found concerning suicidal ideation but not for depression. Main limitations include small sample size and stratified controls. The treatment appears to be safe, feasible and acceptable and initial results show promising trends to support further study of the approach. [ABSTRACT FROM AUTHOR]

Background: Cognitive impairment is considered a central feature of schizophrenia (SZ) and several rehabilitation treatments have been developed to try to improve cognitive deficits. Aims: The aim of the present study was to analyze the effectiveness of integrated psychological therapy (IPT) compared with a standard treatment (TAU) in two groups of patients with SZ, using a comprehensive testing battery of clinical, cognitive, social cognition and functional outcome domains. Methods: Forty-one patients with SZ were assigned to IPT or TAU groups in a randomized controlled trial (RCT). Psychopathological, neuropsychological, emotional and functional outcome variables were assessed at baseline and after 36 weeks of treatment. Results: The IPT group showed significant improvements than the TAU group regarding clinical and functional outcome variables. Moreover, the IPT group improved significantly in the cognitive domains and emotional functioning. Finally, linear regression has highlighted that the improvement of cognitive variables depends on having done the IPT treatment. Conclusions: IPT seems to be effective in improving clinical, neuropsychological, emotional and functional outcome in chronic SZ inpatients. Further studies would be desirable to deepen the effectiveness of IPT in the field of the psychiatric rehabilitation pointing to the possibility of recovery from mental illness. [ABSTRACT FROM AUTHOR]

Background: Interpersonal psychotherapy (IPT) can be effective for both Bulimia Nervosa (BN) and co-occurring depression. While changes in symptoms of Eating disorder (ED) and depression have been found to correlate, it is unclear how they interact during treatment and in which order the symptoms decrease. Methods: Thirty-one patients with BN and depressive symptoms received IPT using the manual IPT-BNm in a naturalistic design. The outcome was measured with the Eating Disorder Examination Questionnaire (EDE-Q) and the Montgomery Åsberg Depression Rating Scale (MADRS-S). Symptom improvement at each session was measured with Repeated Evaluation of Eating Disorder Symptoms (REDS) and the Patient Health Questionnaire-9 (PHQ-9). Results: Significant improvements with large effect sizes were found on both ED symptoms and depression. The rates of change were linear for both BN and depression. A strong correlation between reduction of depressive symptoms and ED symptoms was found. Depressive symptom reduction at one session predicted improvement of ED symptoms at the next session. Conclusions: IPT-BNm had an effect on both BN and co-occurring depressive symptoms. The analyses indicated that reduction in depressive symptoms preceded reduction in bulimic symptoms. [ABSTRACT FROM AUTHOR]

Background: Tuberculosis (TB) and human immunodeficiency virus (HIV) co-infection constitute a deadly infectious disease synergy disease and major public health problem throughout the world. The risk of developing active TB in people living with HIV (PLHIV) is 21 times higher than the rest of the world population. The overlap of latent TB infection and HIV infection has resulted in marked increases in TB incidence in countries with dual epidemics. Although antiretroviral therapy (ART) is the single most significant way to reduce incident TB in PLHIV, besides early ART initiation, isoniazid preventive therapy (IPT) is the key intervention to prevent TB among PLHIV. This prospective cohort and longitudinal study aimed to document; retention, adherence, development of active TB disease, possible adverse drug reactions and completion among patients initiated on IPT in Jan 2019. Methods: This was both a prospective cohort and longitudinal study nested within a national quality improvement collaborative in which multiple quality improvement teams tested changes in care delivery to improve the delivery of IPT. The prospective cohort were HIV patients without TB disease initiated on a dosage of Isoniazid 300 mg/day for adults and 150 mg/day for children for a period of 6 months. Association statistics were used to describe patient characteristics and outcomes. Variables with p-value < 0.05 were used to determine linear by linear associations between patient characteristics assumed to influence both primary and secondary outcomes. Variables with a p-value < 0.05 were included in the logistical regression model. The final model included those factors that retained statistical significance. The odds ratios (OR) and adjusted OR (AOR) along with its 95% confidence interval were used to determine the power of relationship in determining the outcomes of interest. The model was tested for fitness using goodness-of-fit Hosmer–Lemeshow tests. Results: The completion of IPT was at 89%. A significant proportion of patients adhered to treatment (89%) and kept their appointment schedules-retention (89%). All patients (100%) received IPT at each appointment visit. Only 4% of patients experienced side effects of isoniazid (INH) but none of them developed active TB at the end of the 6 month INH dose. Multivariate logistic regression analysis of covariates of IPT completion revealed a strong and statistical association between IPT completion and age, gender, retention and side effects of INH. Our multivariate model found that children below 15 years were less likely to complete INH than patients ≥ 15 years (AOR = 0.416, p = 0.230, df = 1). Female patients were 2 times more likely to complete INH dose than male patients (AOR = 1.598, p = 0.018). Patients who kept all their appointment schedules were 10 times more likely to complete IPT than those who missed one or more schedules (AOR = 10.726, p = 0.000, df = 1). We also found that patients who did not report any side effects associated with INH were 2 times more likely to complete INH (AOR = 1.958, p = 0.016, df = 1) than patients who reported one or more side effects. Conclusion: Treatment completion is the end-point of the IPT initiation strategy in Uganda. With a completion rate of 89%, our results seem re-assuring and suggest that improvement collaborative is an effective approach to achieving results through combined efforts. The high rates of completion are encouraging indicators of progress in the implementation of collaborative activities in the study setting. However, such collaboratives would require periodic evaluation to prevent possible relapses in progress attained. [ABSTRACT FROM AUTHOR]

Studies on inductive power transfer (IPT) systems are most times either theoretical or experimental. In this paper, we want to bring theoretical models and experimental data together using the impedance based interface proposed in SAE J2954. This proposal characterizes the IPT system by impedances at both coil terminals. We show how the experimental data was retrieved at the interface and use it to validate an analytical model and a Simulink model described within this study. Such models can support the design and development process and therefore a comparison with reality is necessary. [ABSTRACT FROM AUTHOR]

The authors describe a case of inflammatory pseudotumour of the maxillary sinus. Though inflammatory in origin, its aggressive clinical pattern and radiological findings resembles malignant disease. Pseudotumours can occur almost anywhere in the body of which very few cases have been reported so far in the maxillofacial region. Our diagnosis in this reported case is well supported by radiological, haematological, histopathological, immunohistochemical and bone marrow findings. The provided conservative treatment was wide local excision, followed by post‐operative steroid therapy to gain adequate control over the aggressive nature of the disease. [ABSTRACT FROM AUTHOR]

Tuberculosis (TB), once widely prevalent throughout the world, experienced falling incidence rates in early twentieth century in developed nations, even before the introduction of anti-TB drugs, attributed to improved hygiene and living conditions. Active TB may develop following fresh infection or activation of latent tuberculosis infection (LTBI). LTBI is a state of persistent bacterial viability, however, the host stays asymptomatic and there is no evidence of clinically active tuberculosis. Therefore, treatment of all LTBI is considered as one of the ways to control tuberculosis. Diagnosis of LTBI relies on presence of immune-reactivity to TB antigen and commonly used tests include tuberculin skin test and interferon gamma release assay (IGRA). At present there is no diagnostic test that can identify an individual with LTBI who will progress to develop active disease or remain asymptomatic. Therefore, it is unclear whom to treat. In the current scenario, treatment for LTBI is restricted to high risk groups which include under-5 y contacts of adults with pulmonary TB. Various regimens for treatment of LTBI are evolving and consist of isoniazid (INH) alone for 6-9 mo or combination of INH and rifampicin for 3-4 mo or once a week combination of rifapentin and INH for 3 mo. There is a need for research to identify LTBI, risk factors for progression of LTBI to active disease and a shorter regimen for treatment. [ABSTRACT FROM AUTHOR]

The article aims to analyse the mechanisms of Indonesian mass killings between 1965 and 1966 in light of the latest reports. The author refers to the most recent academic research, as well as the verdict announced in 2016 by the International People's Tribunal in the Hague on the 1965 Crimes against Humanity in Indonesia (IPT 1965). Available sources confi rm the thesis advanced by the researchers of the Indonesian political upheaval since the early 1980s that mass violence in the archipelago should be understood as a crime against humanity carried out by the military in an organised manner, with a declared intent to annihilate the main political rival, the Indonesian Communist Party (PKI). The author focuses on the issues of the organisation and morphology of violence, its ethnic dimension, as well as the problem of the international and internal negation of genocide in Indonesia, and the related issues of transitional justice [ABSTRACT FROM AUTHOR]

The seed oil of Jatropha curcas is considered a potential bioenergy source that could replace fossil fuels. However, the seed yield of Jatropha is low and has yet to be improved. We previously reported that exogenous cytokinin treatment increased the seed yield of Jatropha. Cytokinin levels are directly regulated by isopentenyl transferase (IPT), cytochrome P450 monooxygenase, family 735, subfamily A (CYP735A), and cytokinin oxidase/dehydrogenase (CKX). In this study, we cloned six IPT genes, one JcCYP735A gene, and seven JcCKX genes. The expression patterns of these 14 genes in various organs were determined using real-time quantitative PCR. JcIPT1 was primarily expressed in roots and seeds, JcIPT2 was expressed in roots, apical meristems, and mature leaves, JcIPT3 was expressed in stems and mature leaves, JcIPT5 was expressed in roots and mature leaves, JcIPT6 was expressed in seeds at 10 days after pollination, and JcIPT9 was expressed in mature leaves. JcCYP735A was mainly expressed in roots, flower buds, and seeds. The seven JcCKX genes also showed different expression patterns in different organs of Jatropha. In addition, CK levels were detected in flower buds and seeds at different stages of development. The concentration of N6-(Δ²-isopentenyl)-adenine (iP), iP-riboside, and trans-zeatin (tZ) increased with flower development, and the concentration of iP decreased with seed development, while that of tZ increased. We further analyzed the function of JcCYP735A using the CRISPR-Cas9 system, and found that the concentrations of tZ and tZ-riboside decreased significantly in the Jccyp735a mutants, which showed severely retarded growth. These findings will be helpful for further studies of the functions of cytokinin metabolic genes and understanding the roles of cytokinins in Jatropha growth and development. [ABSTRACT FROM AUTHOR]

This paper introduces the working principle of the inductive power transfer (IPT) system from the perspective of the electromagnetic field. Using Maxwell's equations, the analytical solution for the electromagnetic field, synthesized by the primary and secondary circular coils in an IPT system, is deduced in detail to obtain the electric field in the IPT system, and the derivation process is easy to understand for researchers engaged in IPT. The final solutions are obtained by combining analytical derivation and the numerical integration method to find the induced voltage in the secondary coil. Finally, by comparison, the simulation results from the finite element software are in a good agreement with those from the analytical analysis. Moreover, an IPT system is set up to validate the analytical and simulation results, and the maximal relative error is under 6% in different working conditions, which shows that it is feasible to understand the working principle of IPT systems from the viewpoint of the electromagnetic field. [ABSTRACT FROM AUTHOR]

Endophytes play crucial roles due to their beneficial influence on plant development, growth, fitness, and diversification. Due to these important capabilities, they have received attention from the scientific community and many papers have been published recently about their beneficial role in in vivo and in vitro plant propagation. However, up to now, there is no research on utilization of these microbial endophytes in prolongation of in vitro storage. Thus, the aim of this study is to assess the influence of fraser photinia associated and putatively endophytic bacterium (Plant Growth Bacteria_ in vitro; PGB_invit) on in vitro storage of its host. When pure strain of the bacterium was inoculated, it enabled the storage of microshoots up to 16 months at 25 °C without requiring periodic subculture while control (unincubated with PGB_invit.) microshoots died after 2 months of storage without subculture as in vitro plant cultures definitely need periodic subcultures (once in every 4-6 weeks) in order to renew media and gaseous atmosphere. Moreover, while the presence of virulence (vir D1), auxin (aux1), and cytokinin (ipt) production genes was confirmed in plasmid DNA of the bacterium, nitrogen fixing gene (nifH) was detected by the PCR analysis using bacterial culture. Overall results demonstrated that with these capabilities PGB_invit could be useful for in vitro conservation of fraser photinia. The novelty is the supplementation of in vitro plant growth without either periodic renewal of the media or decreasing the culture temperature by means of a beneficial plant-bacterium interaction. [ABSTRACT FROM AUTHOR]

The effects of isoprothiolane (IPT: a fungicide used to control rice blast) and nordihydroguaiaretic acid (NDGA: an inhibitor of the phytohormone abscisic acid (ABA) biosynthesis) on the maturation of “Shine Muscat” grape berries were investigated. The results showed that IPT and NDGA retarded chlorophyll degradation in the berry skin. Transcriptional profiling revealed that 2218 genes in the grapes treated with IPT and 2270 genes in those treated with NDGA were differentially expressed compared to the untreated control grapes at 70 days after full bloom (DAFB). IPT or NDGA application regulated the expression of genes involved in ABA, auxin, and ethylene metabolism. MapMan analysis and qRT-PCR validation showed that the expression levels of VvNCED1, VvCYP707A1, VvAAO4, and VvGEM-like genes in the ABA metabolic pathway were negatively regulated by IPT and NDGA treatment at 70 DAFB. Moreover, IPT and NDGA treatment inhibited glucose accumulation via the VvSUS, VvAI, VvHT genes in the sugar metabolic pathway. In addition, IPT and NDGA treatment delayed (E)-2-hexenal and hexanol productions, and down-regulated aroma metabolic pathway genes such as VvLOX, VvADH, VvGPPS, and VvTPS. These results suggest that IPT and NDGA influence chlorophyll degradation, ABA accumulation, and sugar and aroma volatile syntheses in the maturation process. [ABSTRACT FROM AUTHOR]

In this work, we have investigated the impact of micro-silica (SiO2), micro-alumina-tri-hydrate (ATH), and mixture of micro/nano-sized particles (ATH + SiO2) on electrical, tracking and erosion, and water absorption characteristics of silicone rubber (SiR)-based novel composites. SiR-blends with varying concentrations by weight percent (wt%) of ATH and SiO2 were prepared by simple blending in a two-roll mixing mill. Tracking/erosion and water absorption resistance properties of SiR-blends were analyzed using the inclined plane test and water immersion tests, respectively, according to ASTM standards. Dielectric strength, volume and surface resistivity, tracking/erosion resistance, and water penetration resistance properties of SiR composites were determined and discussed. SiR composites with ATH showed enhanced dielectric strength, volume and surface resistivity, erosion resistance, and initial tracking voltage compared to SiO2-filled composites. Further, the ATH and SiO2 co-filled specimens showed better water immersion resistance compared to samples only filled with ATH or SiO2. We presume characterization results presented in this work will help researchers to optimize the performance of SiR for outdoor high-voltage insulation applications. [ABSTRACT FROM AUTHOR]

Cytokinins are important regulators of cell proliferation and differentiation in plant development. Here, a role for this phytohormone group in soybean nodulation is shown through the exogenous application of cytokinins (6-benzylaminopurine, N6-(Δ2-isopentenyl)-adenine and trans -zeatin) via either root drenching or a petiole feeding technique. Overall, nodule numbers were reduced by treatment with high cytokinin concentrations, but increased with lower concentrations. This was especially evident when feeding the solutions directly into the vasculature via petiole feeding. These findings highlight the importance of cytokinin in nodule development. To further investigate the role of cytokinin in controlling nodule numbers, the IPT gene family involved in cytokinin biosynthesis was characterized in soybean. Bioinformatic analyses identified 17 IPT genes in the soybean genome and homeologous duplicate gene partners were subsequently identified including GmIPT5 and GmIPT6 , the orthologs of LjIPT3. Expression of GmIPT5 was upregulated in the shoot in response to nodulation, but this was independent of a functional copy of the autoregulation of nodulation (AON) receptor, GmNARK, which suggests it is unlikely to have a role in the negative feedback system called AON. Legumes also control nodule numbers in the presence of soil nitrogen through nitrate-dependent regulation of nodulation, a locally acting pathway in soybean. Upon nitrate treatment to the root, the tandem duplicates GmIPT3 and GmIPT15 were upregulated in expression indicating a role for these genes in the plant’s response to soil nitrogen, potentially including the nitrate-dependent regulation of legume nodulation pathway. Additional roles for cytokinin and their IPT biosynthetic genes in nodulation and the control of nodule numbers are discussed. [ABSTRACT FROM AUTHOR]

The seed oil of Jatropha curcas is considered a potential bioenergy source that could replace fossil fuels. However, the seed yield of Jatropha is low and has yet to be improved. We previously reported that exogenous cytokinin treatment increased the seed yield of Jatropha. Cytokinin levels are directly regulated by isopentenyl transferase (IPT), cytochrome P450 monooxygenase, family 735, subfamily A (CYP735A), and cytokinin oxidase/dehydrogenase (CKX). In this study, we cloned six IPT genes, one JcCYP735A gene, and seven JcCKX genes. The expression patterns of these 14 genes in various organs were determined using real-time quantitative PCR. JcIPT1 was primarily expressed in roots and seeds, JcIPT2 was expressed in roots, apical meristems, and mature leaves, JcIPT3 was expressed in stems and mature leaves, JcIPT5 was expressed in roots and mature leaves, JcIPT6 was expressed in seeds at 10 days after pollination, and JcIPT9 was expressed in mature leaves. JcCYP735A was mainly expressed in roots, flower buds, and seeds. The seven JcCKX genes also showed different expression patterns in different organs of Jatropha. In addition, CK levels were detected in flower buds and seeds at different stages of development. The concentration of N6-(Δ²-isopentenyl)-adenine (iP), iP-riboside, and trans-zeatin (tZ) increased with flower development, and the concentration of iP decreased with seed development, while that of tZ increased. We further analyzed the function of JcCYP735A using the CRISPR-Cas9 system, and found that the concentrations of tZ and tZ-riboside decreased significantly in the Jccyp735a mutants, which showed severely retarded growth. These findings will be helpful for further studies of the functions of cytokinin metabolic genes and understanding the roles of cytokinins in Jatropha growth and development. [ABSTRACT FROM AUTHOR]

Objectives: There are limited options for depressed patients to have access to evidence-based psychotherapies in the community. This pilot study explored the feasibility of delivering interpersonal psychotherapy (IPT) to clients in a community support agency.Method: A total of 18 clients with depression completed at least eight sessions of IPT (range 8-13) and 17 completed a range of pre- and post-treatment measures.Results: Clients had a high level of depression and were functioning poorly. All found the delivery of IPT in the community useful and would recommend therapy to others. There was a significant reduction in self-report and clinician-rated depression, and improvement in social functioning.Conclusions: This study supports the notion that therapy can be delivered by appropriately trained non-mental-health clinicians in the community with good effect and adds to the range of options for delivery of psychiatric care. [ABSTRACT FROM AUTHOR]

Cytokinins are essential for legume plants to establish a nitrogen-fixing symbiosis with rhizobia. Recently, the expression level of cytokinin biosynthesis IPTs (ISOPENTENYLTRANSFERASES) genes was shown to be increased in response to rhizobial inoculation in Lotus japonicus, Medicago truncatula and Pisum sativum. In addition to its well-established positive role in nodule primordium initiation in root cortex, cytokinin negatively regulates infection processes in the epidermis. Moreover, it was reported that shoot-derived cytokinin inhibits the subsequent nodule formation through AON (autoregulation of nodulation) pathway. In L. japonicus, LjIPT3 gene was shown to be activated in the shoot phloem via the components of AON system, negatively affecting nodulation. However, in M. truncatula, the detailed analysis of MtIPTs expression, both in roots and shoots, in response to nodulation has not been performed yet, and the link between IPTs and AON has not been studied so far. In this study, we performed an extensive analysis of MtIPTs expression levels in different organs, focusing on the possible role of MtIPTs in nodule development. MtIPTs expression dynamics in inoculated roots suggest that besides its early established role in the nodule primordia development, cytokinin may be also important for later stages of nodulation. According to expression analysis, MtIPT3, MtIPT4, and MtIPT5 are activated in the shoots in response to inoculation. Among these genes, MtIPT3 is the only one the induction of which was not observed in leaves of the sunn-3 mutant defective in CLV1-like kinase, the key component of AON, suggesting that MtIPT3 is activated in the shoots in an AON-dependent manner. Taken together, our findings suggest that MtIPTs are involved in the nodule development at different stages, both locally in inoculated roots and systemically in shoots, where their expression can be activated in an AON-dependent manner. [ABSTRACT FROM AUTHOR]