Purpose Various techniques have been described for laparoscopic nephroureterectomy. We reviewed our initial experience of laparoscopic nephroureterectomy with robot-assisted extravesical excision of the distal ureter and bladder cuff.Materials and Methods Nine consecutive patients aged 43 to 83 years underwent laparoscopic nephroureterectomy for transitional cell carcinoma (TCC) between August 2005 and March 2007. The first five patients were repositioned after laparoscopic nephrectomy from flank to lithotomy position to dock the robot for excision of the distal ureter and bladder cuff by a single surgeon. In contrast, the last four patients remained in flank position throughout the entire procedure, with the robot docked in flank position following laparoscopic nephrectomy. A two-layer closure re-approximated the cystotomy and a urethral catheter was left in place for a mean of 5 days.Results Eight men and one woman with a mean age of 64.2 years and mean body mass index (BMI) of 28.4 kgm2underwent flexible cystoscopy and laparoscopic nephroureterectomy for five right-sided and four left-sided tumors. Mean operative time was 303 minutes (range 210–430 minutes), estimated blood loss was 211 mL (range 50–700 mL), and mean length of hospital stay was 2.3 days. Pathologic staging revealed T3for five (55.6), Tafor two (22.2), carcinoma in situ(CIS) for two (22.2) patients, and high-grade disease for seven (77.8) patients. With a mean follow-up of 16.2 months (range 4.3–24.3 months), three patients with a history of bladder cancer have experienced recurrence in the bladder, and one of the three has also developed metastatic disease.Conclusions Laparoscopic nephroureterectomy with robot-assisted extravesical excision of the distal ureter and bladder cuff appears to be a feasible alternative for patients with TCC of the upper urinary tract. [ABSTRACT FROM AUTHOR]