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The article focuses on findings of a study on use of ADAM-rWBC for counting residual leucocytes in leucocyte-reduced whole blood and apheresis platelet concentrates. It states that ADAM-rWBC from NanoEnTek can count residual leucocytes in blood components for transfusion, and mentions that emission images of the cells are detected by charge coupled device (CCD) camera. It notes that regression analyses showed linearity between the expected values and observed values of ADAM-rWBC.

Hollow optical fiber probes are used for remote Raman and infrared spectroscopy. Preliminary experiments using these probes are performed, and the results of the experiments show that the hollow fiber probes are useful for endoscopic measurement of Raman and infrared spectroscopy. As regards to Raman spectroscopy, a hollow fiber probe with a ball lens at the end works as a confocal system. It can, thus, detect the molecular structure of biotissues with a high SNR. Owing to their small diameter, the probes are useful for in vivo noninvasive analysis by using a flexible endoscope. A hollow-fiber probe was also used for remote Fourier transform infrared spectroscopy in the form of an endoscopic measurement of infrared reflectometry spectra inside the body. This measurement was made possible by the high flexibility and durability, nontoxicity, and low transmission losses of the hollow-fiber-based probes. [ABSTRACT FROM PUBLISHER]

We investigated the activity and the internal motions of a stabilized mutant hen lysozyme (HEL) in which the residues M12 and L56 were mutated to L and F, respectively (LF mutant HEL). The result of the activity measurements against glycol chitin at various temperatures suggested that the temperature dependence of the activity of LF mutant HEL shifted to the high-temperature side compared with that of wild-type HEL. The detailed internal motions of LF mutant HEL in the absence and presence of a substrate analogue, (NAG)3, were examined by model-free analysis at 35°C. The results showed that the internal motions of LF mutant HEL in the presence of (NAG)3 were drastically restricted compared with those in wild-type HEL. Our findings thus suggested that the mutation to the stabilized lysozyme restricted internal motions required for the enzymatic reaction. [ABSTRACT FROM AUTHOR]

A 73-year-old man with a papillary adenoma located in the distal common bile duct is reported. He underwent pylorus-preserving pancreatoduodenectomy. The lesion in the common bile duct featured papillary proliferation of the epithelium and fibrous elements with diffuse infiltration by inflammatory cells. Positive staining for MIB-1 (Ki-67) and p53 was identified in the nuclei of the proliferative epithelium. These findings suggested the malignant potential of this lesion. Further progress in imaging diagnostic techniques should increase the frequency with which such lesions are discovered. Even now, if mural irregularities and defects are found in the extrahepatic biliary system, especially the distal common bile duct, the possibility of such borderline biliary adenoma should be taken into consideration when making a diagnosis. [ABSTRACT FROM AUTHOR]

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