1. Abundance and origins of plasma lipids in sexually maturing female coho salmon Oncorhynchus kisutch (Walbaum) in culture.
- Author
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Johnson, Ronald B, Kroeger, Eric L, Reichert, William L, Deavila, David M, and Rust, Michael B
- Subjects
COHO salmon ,BLOOD lipids ,LIPOPROTEINS ,LOW density lipoproteins ,HIGH density lipoproteins ,VITELLOGENESIS - Abstract
Coho salmon Oncorhynchus kisutch are semelparous spawners with highly synchronized oocyte growth. Plasma was collected from maturing female fish and plasma lipids were quantified by lipoprotein class. Stable
13 C isotopes were employed to investigate the origins of plasma lipids. Plasma lipoproteins were partitioned into the very low density ( VLDL), low density ( LDL), high density ( HDL) and very high density ( VHDL) lipoprotein classes. Lipids from all lipoprotein classes increased between the lipid droplet and early vitellogenesis stages of oocyte growth and VHDL lipids continued to increase through mid vitellogenesis ( P < 0.05). During vitellogenesis, total plasma lipid concentrations were similar to the sum of lipoprotein lipids ( P = 0.51). Plasma vitellogenin determined by an ELISA method was very well correlated with VHDL lipid during vitellogenesis ( R2 = 0.91, n = 15). In general, the δ13 C values of plasma lipids reflected that of feed (exogenous) and muscle (endogenous) lipids when feed and muscle δ13 C values were similar, and were intermediate when differences existed between the two lipid sources. With one exception, the δ13 C values of lipids from all lipoprotein classes within a plasma sample were similar. Results indicate that during sexual maturity, lipids from all plasma lipoprotein classes originate from a common pool of exogenous and endogenous lipids. [ABSTRACT FROM AUTHOR]- Published
- 2014
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