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The chemical composition of the essential oils of two plants (Tagetes minuta L. and Lavandula coronopifolia L.) harvested from the Day region (in the north of Djibouti) is the subject of this study. The extraction of essential oils was carried out by hydrodistillation, and the average yield was obtained at a rate of approximately 0.25% for Tagetes minuta L. and 0.42% for Lavandula coronopifolia L. The analyses of these essential oils by gas chromatography coupled with mass spectrometry identified 13 compounds in the essential oil of Tagetes minuta L., including dihydrotagetone (20.8%), artemisia (17.9%), (Z)-tagetenone (12.4%), (-)-spathulenol (11.0%) and estragole (9.5%), were obtained as majority compounds, with a percentage of 71.6%. The essential oil of Lavandula coronopifolia L. is characterized by the presence of 42 compounds, including cis-caryophyllene (18.9%), dehydronerolidol (12.8%), isolongifolanone (11.2%), caryophyllene oxide (8.2%), 10-epi-β-eudesmol (7.7%) and humulene (5.1%), were obtained as the majority chemical constituents, with a percentage of 63.9%. The antimicrobial activities of the essential oils at concentrations of 5% were measured against 12 bacterial strains (Gram positive: Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Streptococcus agalactiae (ATCC 27956), Staphylococcus epidermidis and Corynebacterium sp.; Gram Negative: Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 700603), Acinetobacter baumannii (ATCC 19606), Shigella sonnei (ATCC 9290), Salmonella enterica sv. Typhimurium (ATCC 13311) and Enterobacter cloacae), and the results of in vitro experiments showed inhibitory effects against most strains tested except Staphylococcus aureus, Enterococcus faecalis and Streptococcus agalactiae. Additionally, both oils were tested for their ability to selectively kill 13 human cancer cells (K562, A549, HCT116, PC3, U87-MG, MIA-Paca2, HEK293, NCI-N87, RT4, U2OS, A2780, MRC-5 and JIMT-T1), and the results obtained, according to the values of IC50, show the significant activity of two essential oils, particularly on the HCT116 and A2780 lines, which present values between 0.25 μg/mL and 0.45 μg/mL, respectively. [ABSTRACT FROM AUTHOR]