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It has been reported that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can result in long-term neurological symptoms such as cognitive dysfunction, however the specific mechanisms underlying this phenomenon remain unclear. Initially, we confirmed a reduction in the level of synaptic proteins in SH-SY5Y neurons following SARS-CoV-2 infection. SARS-CoV-2 Nsps are crucial for the efficient replication of the virus and play important roles in the interaction between virus and host cell. Nsps screening experiments implied that Nsp7 is able to reduce the level of synapsin-1. Furthermore, overexpression of Nsp7 in SH-SY5Y cells and mouse primary neurons demonstrated that Nsp7 could decrease the levels of synaptic proteins without affecting neuronal viability. Moreover, C57BL/6 mice receiving AAV-GFP-Nsp7 injections into the ventral hippocampus displayed impaired memory ability, along with reduced dendritic spine density and synaptic protein levels. Mechanistic investigations suggested that Nsp7-induced mitochondrial damage led to ROS production and ATP levels decreasing in neurons. Additional experiments employing the ROS inhibitor NAC demonstrated that Nsp7 suppressed the expression of synaptic proteins via ROS inducing, implicating mitochondrial dysfunction in synaptic plasticity impairment and subsequent cognitive dysfunction. Our findings underscore the crucial role of SARS-CoV-2 Nsp7 in cognitive dysfunction, which is potentially mediated through impaired synaptic plasticity via mitochondrial damage. This study enhances our understanding of the pathogenic mechanisms underlying central nervous system-related symptoms associated with SARS-CoV-2 infection. [ABSTRACT FROM AUTHOR]

Electrochemical CO2 reduction (ECR) to value-added products is regarded as a sustainable strategy to mitigate global warming and energy crisis, and designing highly efficient and robust catalysts is essential. In this work, transition metal sulfides (TMS)-decorated CuS microflower-like structures were prepared via the one-pot hydrothermal synthesis method for ECR to CO, and the influence of TMS doping on ECR performance was demonstrated. Characterization of the catalysts was performed using XRD, FESEM-EDS, N2 physisorption, and XPS, revealing the successful loading of TMS, the formation of microflower-like architectures and the generation of sulfur vacancies. Electrochemical tests demonstrated that doping ZnS, Bi2S3, CdS and MoS2 improved the intrinsic CO2 reduction activity of the CuS catalyst. Particularly, the MoS2-CuS composite catalyst with imperfect petal-like structure showed uniform distribution of edge Mo sites, which worked synergistically with the formed grain boundaries (GBs) and undercoordinated S vacancy sites in promoting CO2 activation, stabilizing *COOH adsorption, facilitating *CO desorption, and lowering the energy barrier of the potential-limiting step for improved CO selectivity. The MoS2-CuS catalyst achieved a maximum CO selectivity of 83.2% at –0.6 V versus the reversible hydrogen electrode (RHE) and a high CO cathodic energetic efficiency of 100%. At this potential, the catalyst maintained stable catalytic activity and CO selectivity during a 333-min electrolysis process. The findings will offer a promising avenue for the development of efficient and stable catalysts for CO production from ECR. [ABSTRACT FROM AUTHOR]

The Ordovician Wulalike Formation in the northwestern Ordos Basin is a new prospect for exploring marine shale gas in China, facing prominent problems such as unclear reservoir conditions and the distribution of enrichment areas. The types of reservoir space, fracture development, porosity composition, and physical properties of the lower Wulalike Formation are discussed through the multi-method identification and quantitative evaluation of reservoir space for appraisal wells. The Wulalike Formation in the study area contained fractured shale reservoirs with matrix pores (mainly inorganic pores) and permeable fractures. The fracture system of the lower Wulalike Formation is dominated by open bed-parallel fractures that are intermittent or continuous individually, with a width of 0.1–0.2 mm and spacing of 0.5–14.0 cm. The fracture-developed intervals generally exhibit bimodal or multimodal features on NMR T2 spectra and have a dual-track feature with a positive amplitude difference in deep and shallow resistivity logs. The length and fracture porosity of fracture-developed intervals varied greatly in different parts of the study area. In the Majiatan-Gufengzhuang area in the southern part of the study area, the fracture development degree generally decreased from west to east. In the Shanghaimiao area in the central part of the study area, fractures were extremely developed, the continuous thickness of the fracture-developed interval was generally more than 20 m, and the average fracture porosity was higher than 1.3%. In the Tiekesumiao area in the northern part of the study area, the fracture development degree was generally lower than that in the central and southern parts of the study area and also showed a decreasing trend from west to east. The lower Wulalike Formation had a total porosity of 2.46–7.08% (avg. 4.71%), roughly similar to the Longmaxi Formation in the Sichuan Basin, of which matrix porosity accounts for 34.0–90.0% (avg. 61.1%) and fracture porosity accounts for 10.0–66.0% (avg. 38.9%). From this, it could be inferred that the shale gas accumulation type of the lower Wulalike Formation in the northwest margin of the basin is mainly a fractured shale gas reservoir controlled by structure, and its "sweet spot area" is mainly controlled by tectonic setting and preservation conditions. This indicates that the Wulalike Formation in the northwestern Ordos Basin has good shale gas exploration prospects, and a large number of fault anticlines or fault noses formed by reverse dipping faults have the potential of favorable exploration targets. [ABSTRACT FROM AUTHOR]

A growing number of studies have shown the benefits of K-12 integrated science and engineering education. With this study, we add to the literature by documenting the relationship between STEM learning and engagement, and the demographic characteristics that impact achievement in STEM. This longitudinal study followed a diverse group of 245 middle school students from sixth grade to eighth grade. Students in two cohorts, cohort I and cohort II, participated in three different integrated STEM units during middle school, one in each grade level. Each curriculum unit focused on the same engineering concepts and practices but addressed different science content standards. The students completed science content tests and engagement surveys before and after the curriculum units. The results of the mixed-effects model analysis showed that behavioral engagement and career inspiration played a critical role in student achievement. While the achievement gap between students of color (SOC) and their White peers was narrowed in cohort II, the achievement gap between the SOC and their White peers in cohort I remained. Our analysis also revealed no gender differences in student achievement. These findings can inform the design of curriculum interventions that specifically target closing the achievement gaps between different groups of students. [ABSTRACT FROM AUTHOR]

This document is a correction notice for an article titled "Ergosterol Isolated from Antrodia camphorata Suppresses LPS-Induced Neuroinflammatory Responses in Microglia Cells and ICR Mice" published in the journal Molecules. The correction addresses errors in Figures 4B, 4C, 4H-J, 5C, and 5D of the original article. The authors apologize for the inconvenience caused but state that the scientific conclusions remain unaffected. The corrected figures have been provided, and the original publication has been updated. [Extracted from the article]

Inflammation caused by microglial activation is important in neurodegenerative diseases. In this research, we tried to identify safe and effective anti-neuroinflammatory agents by screening a natural compounds library and found that Ergosterol can inhibit the nuclear factor kappa-light-chain enhancer of the activated B cells (NF-κB) pathway induced by lipopolysaccharide (LPS) in microglia cells. Ergosterol has been reported to be an effective anti-inflammatory agent. Nevertheless, the potential regulatory role of Ergosterol in neuroinflammatory responses has not been fully investigated. We further investigated the mechanism of Ergosterol that regulates LPS-induced microglial activation and neuroinflammatory reactions both in vitro and in vivo. The results showed that Ergosterol can significantly decrease the pro-inflammatory cytokines induced by LPS in BV2 and HMC3 microglial cells, possibly by inhibiting the NF-κB, protein kinase B (AKT), and mitogen-activated protein kinase (MAPK) signaling pathways. In addition, we treated Institute of Cancer Research (ICR) mice with a safe concentration of Ergosterol following LPS injection. Ergosterol treatment significantly decreased microglial activation–associated ionized calcium-binding adapter molecule-1 (IBA-1), NF-κB phosphorylation, and pro-inflammatory cytokine levels. Moreover, Ergosterol pretreatment clearly reduced LPS-induced neuron damage by restoring the expression of synaptic proteins. Our data may provide insight into possible therapeutic strategies for neuroinflammatory disorders. [ABSTRACT FROM AUTHOR]

Background: Acute lymphoblastic leukemia (ALL) is a type of heterogeneous hematopoietic malignancy that accounts for approximately 20% of adult ALL. Although ALL complete remission (CR) rate has increased to 85–90% after induction chemotherapy, 40–50% of patients eventually relapsed. Therefore, it is necessary to improve the outcomes of ALL via accurate diagnosis and individualized treatments, which benefits in part from molecular biomarkers. Here, we identified a new fusion gene, Acyl-CoA Thioesterase 7–Nephrocystin 4 (ACOT7–NPHP4), in a 34-year-old patient with ALL. The fusion gene contributed to chemoresistance to doxorubicin and acted as a new molecular marker. Case presentation: A 34-year-old male patient was diagnosed with ALL (common B cell) based on clinical manifestations and laboratory results. Although the patient received two cycles of the hyper-CVAD-L regimen as chemotherapy, the induction treatment failed. Because of the refusal of further treatments, the patient died of rapid progression of ALL one month later. Finally, a new fusion transcript, ACOT7–NPHP4, was detected in the patient's lymphoblastic leukemia cells via RNA sequencing. Conclusion: This is the first report of a patient with ALL carrying an ACOT7–NPHP4 fusion gene. These findings may help understand the impact of ACOT7–NPHP4 in clinical molecular monitoring and drug resistance to doxorubicin; furthermore, its leukemogenesis will be essential to explore in future. [ABSTRACT FROM AUTHOR]

Objectives: The purpose of this study was to explore the clinical application value of phase angle (PA) of six parts in the nutritional evaluation and construct a prediction model for diagnosing malnutrition of tumor patients.Methods: A total of 1129 patients with malignant tumors were analyzed retrospectively. The age, sex, tumor location and body mass index (BMI) of the patients were collected, and PA of six parts was measured. The Patient Subjective Global Assessment (PG-SGA) was used to evaluate the nutritional status of each patient.Results: According to the PG-SGA, 66.5% (n = 750) of the patients were evaluated as malnourished. Patients under the age of 65 had higher PA values. The PA value of men was higher than that of women (except PA-RL). In different disease groups, the PA-RA and PA-TR values were significantly different. In our study, PA value increases with BMI and decreases with PG-SGA (except PG-SGA 0-1 group). Multivariate regression analysis indicates that the age (HR = 1.051, 95% CI 1.037-1.066, P < 0.001), BMI (HR = 0.885, 95% CI 0.849-0.924, P < 0.001), and PA-WB (HR = 0.615, 95% CI 0.546-0.692, P < 0.001) were independent significant predictors associated with malnutrition. The AUC of the prediction model is 0.7631 (p < 0.001), indicating that the model including age, BMI, and PA-WB has certain diagnostic value for the diagnosis of malnutrition.Conclusion: The PA-WB is an independent prognostic factor of malnutrition. The prediction model constructed by age, BMI, and PA-WB can be used as a useful tool for nutritional evaluation of tumor patients.Trial Registration: Clinical Trial No.: ChiCTR2100047858. [ABSTRACT FROM AUTHOR]

Recently, the global incidence of gastric/gastroesophageal junction (G/GEJ) cancer has remained high. China is also a large country with a high gastric cancer (GC) incidence rate, where the cases of GC account for 40% of all cases worldwide. More than 90% of GEJ cancers are the adenocarcinoma pathological type. Patients with early-stage G/GEJ adenocarcinoma may have a better prognosis after surgery. In contrast, patients with advanced metastatic G/GEJ adenocarcinoma usually choose comprehensive treatment based on systemic pharmacotherapy, but the subsequent long-term survival is not optimistic. The discovery of various biomarkers, especially microsatellite instability (MSI), programmed cell death-ligand 1 (PD-L1), human epidermal growth factor receptor 2 (HER2), tumor mutational burden (TMB) and Epstein–Barr virus (EBV), has led to the identification of an increasing number of targeted populations and has greatly improved the clinical efficacy of treatments for G/GEJ adenocarcinoma. The ToGA trial added trastuzumab to standard chemotherapy, showed improved survival of patients with HER2-positive advanced G/GEJ adenocarcinoma and brought these patients into a new era of HER2-targeted therapy. Moreover, many HER2-targeted agents have been developed and studied in patients with advanced HER2-positive G/GEJ adenocarcinoma who have demonstrated excellent clinical outcomes. However, many patients experience disease progression with HER2-targeted therapy; hence, new anti-HER2 drugs keep being developed, significantly reducing HER2 resistance. This paper reviews HER2-targeted drugs for advanced metastatic G/GEJ adenocarcinoma, potential resistance mechanisms and future directions. [ABSTRACT FROM AUTHOR]

The gas holdup reduced from 0.62 to 0.31 when the L/S ratio increased from 5 mL/g to 15 mL/g at I U i SB I g i sb = 0.082 m/s. It also coincided with the results that the increased L/S ratio promoted the bubble coalescence which can reduce the gas holdups. The gas holdup increased when the gas velocity became greater at L/S = 5-25 mL/g. The standard deviation of the gas holdup was 2.8% at I U i SB I g i sb = 0.0041 m/s. The L/S ratio exerted no significant effect on the gas holdup at the low gas velocity ( I U i SB I g i sb = 0.0041 m/s). Keywords: bubble column; carbide slag; direct aqueous carbonation; gas-liquid interfacial area EN bubble column carbide slag direct aqueous carbonation gas-liquid interfacial area 765 778 14 07/21/22 20220701 NES 220701 1 Introduction The direct aqueous carbonation is one of the routes of the accelerated carbonation for the CO SB 2 sb capture ([43]). The gas holdup, bubble diameter, bubble residence time, and gas-liquid interfacial area were the important parameters, which can describe the gas dispersion and the bubble characteristics in the bubble column systems ([1]; [27]; [31]; [51]; [56]; [69]). The bubble behaviors were characterized by the bubble formation, bubble shape, bubble rise velocity, bubble induced liquid-solid flow, particle entrainment and drift effect, bubble coalescence and breakup, and bubble size distribution ([63]). [Extracted from the article]

Hypertensive disorders complicating pregnancy (HDCP) are common pregnancy-related disorders. In this study, we aimed to study the clinical value of flow-mediated dilation (FMD) in HDCP and its association with endothelial dysfunction and HDCP-related factors. 160 HDCP patients and 120 healthy pregnancies were enrolled in the study. The expressions of endothelial function markers and FMD were determined. In addition, their correlations in HDCP patients were also analyzed using Pearson's correlation analysis. FMD value decreased gradually from normal pregnancy to severe PE. The levels of plasma nitric oxidase (NO) were significantly lower in the HDCP group than those in the control group, while the levels of plasma endothelin-1 (ET-1) were increased dramatically in the HDCP group. Moreover, the levels of placental growth factor (PLGF) in HDCP women were significantly lower, while the soluble FMS-like tyrosine kinase 1 (sFLt-1) levels were markedly higher than those in control. In addition, the FMD value was correlated with the levels of plasma NO, ET-1, PLGF and sFlt1. It was also found that lower levels of FMD correspond to endothelial dysfunction and abnormal concentrations of PLGF and sFlt-1. The FMD value was associated with endothelial function indicators and could be a strong and non-invasive measure to predict HDCP. The association between the FMD values and endothelial function indicators in HDCP could be helpful for the prediction of pregnant hypertension more accurately. [ABSTRACT FROM AUTHOR]

Background: Rearrangements involving the fibroblast growth factor receptor 1 (FGFR1) gene result in 8p11 myeloproliferative syndrome (EMS), which is a rare and aggressive hematological malignancy that is often initially diagnosed as myelodysplastic syndrome (MDS). Clinical outcomes are typically poor due to relative resistance to tyrosine kinase inhibitors (TKIs) and rapid transformation to acute leukemia. Deciphering the transcriptomic signature of FGFR1 fusions may open new treatment strategies for FGFR1 rearrangement patients. Methods: DNA sequencing (DNA-seq) was performed for 20 MDS patients and whole exome sequencing (WES) was performed for one HOOK3-FGFR1 fusion positive patient. RNA sequencing (RNA-seq) was performed for 20 MDS patients and 8 healthy donors. Fusion genes were detected using the STAR-Fusion tool. Fluorescence in situ hybridization (FISH), quantitative real-time PCR (qRT-PCR), and Sanger sequencing were used to confirm the HOOK3-FGFR1 fusion gene. The phosphorylation antibody array was performed to validate the activation of nuclear factor-kappaB (NF-kappaB) signaling. Results: We identified frequently recurrent mutations of ASXL1 and U2AF1 in the MDS cohort, which is consistent with previous reports. We also identified a novel in-frame HOOK3-FGFR1 fusion gene in one MDS case with abnormal monoclonal B-cell lymphocytosis and ring chromosome 8. FISH analysis detected the FGFR1 break-apart signal in myeloid blasts only. qRT-PCR and Sanger sequencing confirmed the HOOK3-FGFR1 fusion transcript with breakpoints located at the 11th exon of HOOK3 and 10th exon of FGFR1, and Western blot detected the chimeric HOOK3-FGFR1 fusion protein that is presumed to retain the entire tyrosine kinase domain of FGFR1. The transcriptional feature of HOOK3-FGFR1 fusion was characterized by the significant enrichment of the NF-kappaB pathway by comparing the expression profiling of FGFR1 fusion positive MDS with 8 healthy donors and FGFR1 fusion negative MDS patients. Further validation by phosphorylation antibody array also showed NF-kappaB activation, as evidenced by increased phosphorylation of p65 (Ser 536) and of IKBalpha (Ser 32). Conclusions: The HOOK3-FGFR1 fusion gene may contribute to the pathogenesis of MDS and activate the NF-kappaB pathway. These findings highlight a potential novel approach for combination therapy for FGFR1 rearrangement patients. [ABSTRACT FROM AUTHOR]

A non-negative latent factor analysis (NLFA)-based recommender can make precise recommendations by correctly representing the non-negative characteristic of industrial data. It commonly relies on a nonconvex and bilinear optimization process, where the effects of first-order solvers maybe significantly reduced. Higher order solvers like a Newton-type method are expected to make a breakthrough; however, its computation efficiency and scalability are greatly limited due to the numerous parameters involved in a Hessian matrix. To address this issue, this article proposes an approach for assimilating second-order information for building NLFA-based recommenders. The key idea is an inner second-order solver that employs a Hessian-free method for avoiding the highly expensive manipulations of a Hessian matrix. Empirical studies on eight data cases emerging from real industrial applications indicate that the proposed approach outperforms state-of-the-art models in prediction accuracy with affordable computational burden. [ABSTRACT FROM AUTHOR]

The impacts of the solid volume fraction Vs and particle size dp on the total and local gas holdups in gas‐liquid‐solid bubble columns under different flow regimes were investigated. The flow regimes discussed were the discrete bubble regime (DBR), transition regime (TR), bubble coalescence regime (BCR), and strong turbulent regime (STR). The results demonstrated that the bed average gas holdup εg decreased with higher solid volume fraction in DBR, TR, and BCR. The increment of the solid volume fraction promoted bubble coalescence and also can enhance the particle effect on the average gas holdup. The εg changed slightly when shifting the Vs or dp in STR. A new correlation for εg was developed considering the particle properties and the flow regimes studied. [ABSTRACT FROM AUTHOR]

The COVID-19 is an infectious disease caused by SARS-CoV-2 infection. A large number of clinical studies found high-level expression of pro-inflammatory cytokines in patients infected with SARS-CoV-2, which fuels the rapid development of the disease. However, the specific molecular mechanism is still unclear. In this study, we found that SARS-CoV-2 Nsp5 can induce the expression of cytokines IL-1β, IL-6, TNF-α, and IL-2 in Calu-3 and THP1 cells. Further research found that Nsp5 enhances cytokine expression through activating the NF-κB signaling pathway. Subsequently, we investigated the upstream effectors of the NF-κB signal pathway on Nsp5 overexpression and discovered that Nsp5 increases the protein level of MAVS. Moreover, Nsp5 can promote the SUMOylation of MAVS to increase its stability and lead to increasing levels of MAVS protein, finally triggering activation of NF-κB signaling. The knockdown of MAVS and the inhibitor of SUMOylation treatment can attenuate Nsp5-mediated NF-κB activation and cytokine induction. We identified a novel role of SARS-CoV-2 Nsp5 to enhance cytokine production by activating the NF-κB signaling pathway. [ABSTRACT FROM AUTHOR]

Academic journals and discipline construction in colleges are two major drivers of scientific progress. There is a complex interactive mechanism between the two drivers. The synergy between the two is worthy of in-depth research. The research aims include better developing academic journals with discipline advantages and back-feeding discipline construction with academic journals. The symbiotic theory provides a new research perspective for the cooperation mechanism between college academic journals and discipline construction. Drawing on the symbiotic theory, this paper explores the root causes and interactive logic of the collaboration between college academic journals and discipline construction, from the angles of the symbiotic unit, symbiotic environment, and symbiotic model. The results show that the symbiosis between academic journals and discipline construction in Chinese colleges mainly relies on two essential parameters, namely, academic talents and research results; although colleges provide a positive environment, the symbiosis between the two factors mostly takes place on unofficial information platforms and cooperation mechanisms, for the relevant policies are relatively new and mostly discipline-oriented; the symbiosis between college academic journals and discipline construction is mutualistic but remains in the intermittent stage. Therefore, it is important to find a more effective symbiotic path for college academic journals and discipline construction, following their symbiotic law. [ABSTRACT FROM AUTHOR]

Ovarian cancer is the most common and aggressive type of tumor of the female reproductive system. Two factors account for this detrimental clinical presentation: (i) the lack of early detection methods and (ii) the inherently aggressive nature of this malignancy. Currently, transcriptomic analyses have become important tools to identify new targets in different cancer types. In this study, by measuring expression levels in ovarian cancer samples and stem cell samples, we identified 24 tumor suppressor genes consistently associated with poor prognosis. Combined results further revealed a potential therapeutic candidate, BTG2, which belongs to the antiproliferative gene family. Our results showed that BTG2 expression regulated ovarian cancer cell proliferation via G1/S phase cell cycle arrest by regulating Cyclin D1, CDK4, p-AKT, and p-ERK expression. BTG2 also inhibited cell migration by modulating MMP-2 and MMP-9 expression. Furthermore, xenograft models confirmed a growth inhibitory effect of BTG2 in ovarian cancer in vivo. BTG2 was significantly associated with ovarian cancer FIGO stage and grade in the clinic. Our findings indicated that BTG2 exerts a suppressive impact on ovarian cancer and could be a potential biomarker. [ABSTRACT FROM AUTHOR]

Atrial fibrillation (AF) is a condition that heart beats quaveringly or irregularly, which causes blood clots, heart failure, stroke, and other heart-related complications. Therefore, early diagnosis and timely preventions are necessary for AF treatment. Compelling evidence indicated that microRNAs (miRNAs) become emerging biomarkers of AF; thus, we aimed to investigate the possibility of miR-455-5p as an AF marker to provide a new strategy for early diagnosis of AF. A minipump containing angiotensin II was implanted into mice to induce AF, and adeno-associated virus (AAV) carrying anti-miR-negative control (NC) or anti-miR-455-5p was injected into the pericardial space of mice respectively. Next, myocytes isolated from wild-type newborn mice were stimulated with angiotensin II and anti-miR-NC or anti-miR-455-5p mimic. The results showed that the expression of miR-455-5p was positively correlated with the severity of AF, and miR-455-5p mimic accelerated the progression of AF by directly binding to its target gene suppressor of cytokines signaling 3 (SOCS3), leading to the activation of signal transducer and activator of transcription 3 (STAT3) signaling pathway. On the contrary, inhibition of miR-455-5p expression effectively ameliorated AF. In conclusion, miR-455-5p might serve as a biomarker of AF. [ABSTRACT FROM AUTHOR]

A mathematical model for the formation of condensation aerosols from highly supersaturated vapor-air mixtures is presented. The model is suitable for systems with very high nucleation rates that result in aerosol mass densities >1 g/m3. It accounts for nucleation, non-isothermal condensation, coagulation and indirectly for coalescence of the newly formed particles. It applies kinetic theory to determine the nucleation rate and treats nucleation and coagulation in separate stages, but both are coupled with non-isothermal condensation. A statistical approach based on the Weibull function is used to estimate the size distribution of the droplets (particles). The model has been applied to study the formation of glycerol aerosols in Electronic Nicotine Delivery Systems. The model qualitatively reproduces the observed trend that the aerosol particle size decreases as the puff flow rate increases. Within the model framework, at lower puff flow rates higher particle concentrations occur leading to higher rates of coagulation which result in larger particle size aerosol. Copyright © 2021 American Association for Aerosol Research [ABSTRACT FROM AUTHOR]

Electrochemical CO2 reduction to value‐added chemicals and fuels using renewable energy represents a promising strategy for reducing CO2 emissions and achieving effective energy storage. In this work, nano‐sized CuO catalysts were prepared by using the homogeneous precipitation method for electrochemical CO2 reduction to CO. The CO2 reduction studies combined with the characterization results show that structure‐activity relationships of the CuO catalysts depend on the calcination temperature. The as‐synthesized CuO catalysts calcined at different temperatures are capable of reducing CO2 to generate syngas with tunable CO/H2 ratios of 1 : 2 to 2 : 1. The desired CuO‐400 catalyst exhibits good morphology, small particle size, and enriched oxygen‐vacancy defects and, therefore, shows good performance for electrochemical CO2 reduction to CO. Under the given potential of −0.93 V vs. RHE, CuO‐400 exhibits good reduction activity and selectivity with a great electrochemically active surface area normalized CO partial current density of 1.44 mA/cm2 and a high CO faradaic efficiency of 48.2 %. The deactivation of CuO‐400 in the long‐term test is associated with the increase in particle size, the reduction of CuO to Cu2O and metallic Cu, and the coverage of surface active sites by the formed carbonate species. Our findings indicate that CuO catalysts with tunable physicochemical properties are promising candidates for electrochemical CO2 reduction to produce syngas. [ABSTRACT FROM AUTHOR]

Measuring the aerosol particle size of electronic cigarettes can be particularly challenging due to the volatile and dynamic nature of these aerosols. In particular, electronic cigarette aerosol particle size measurements may be convoluted by evaporation due to the high dilution ratios and coagulation when there are long residence times between sampling and measurement. To address these measurement issues, we have developed a particle size measurement system using a low flow cascade impactor with a sampling system that is capable of generating a puff on an e-cigarette and directly introducing the aerosol into the inlet flow of the impactor. The system has been used to investigate factors that may affect the aerosol size distribution, including puffing conditions, carrier composition and device construction. It was found that decreasing the puff flow rate from 2 to 0.5 L/min at a constant puff duration of 5 s, the mass median aerodynamic diameter (MMAD) of the aerosol size distribution increased from 0.5 to 1.1 µm. Increasing the mass delivery from 0.67 to 6 mg/5 s puff also resulted in increasing MMAD from 0.48 to 0.87 µm. Furthermore, the addition of a small amount of glycerin (1% by weight) to a propylene glycol based flavor formulation caused a decrease in the MMAD, while the other factors studied did not have a significant effect on the MMAD. Copyright © 2020 American Association for Aerosol Research [ABSTRACT FROM AUTHOR]

This research was designed to investigate if antioxidant sulforaphane (SFN) alleviates methamphetamine (METH)-induced neurotoxicity by activation of the Nrf2-mediated pathway. Oxidative damage, apoptosis and mitochondrial membrane potential were evaluated in PC12 cells or the prefrontal cortex of SD rats post METH treatment with/without SFN. In addition, knockdown of Nrf2 expression in PC12 cells was used to further study the underlying mechanism. The results showed that METH exposure induced increased oxidative damage and apoptosis accompanied by decreased expressions of Nrf2, HO-1 and GCS in PC12 cells and the prefrontal cortex of SD rats. Meanwhile SFN effectively prevented METH-induced cell oxidative damage, apoptosis and mitochondrial membrane potential; however, the knockdown of Nrf2 expression partly reversed the protective effect of SFN in reducing METH-induced oxidative damage and apoptosis in PC12 cells. These results indicate that SFN might be a promising functional food-derived compound for preventing METH-induced neurotoxicity via the Nrf2-mediated pathway. [ABSTRACT FROM AUTHOR]

Dihydrotanshinone I (DHTS), extracted from Salvia miltiorrhiza, was found to be the most effective compound of tanshen extracts against cancer cells in our previous studies. However, the therapeutic benefits and underlying mechanisms of DHTS on ovarian cancer remain uncertain. In this study, we demonstrated the cytocidal effects of DHTS on chemosensitive ovarian cancer cells with or without platinum‐based chemotherapy. DHTS was able to inhibit proliferation and migration of ovarian cancer cells in vitro and in vivo through modulation of the PI3K/AKT signalling pathways. Combinatorial treatment of DHTS and cisplatin exhibited enhanced DNA damage in ovarian cancer cells. Overall, these findings suggest that DHTS induces ovarian cancer cells death via induction of DNA damage and inhibits ovarian cancer cell proliferation and migration. [ABSTRACT FROM AUTHOR]

Background: Although several long non-coding RNAs (lncRNAs) have been found to be involved in gastric cancer tumorigenesis, the more comprehensive contributions of lncRNAs to gastric cancer require further investigation. Here, we identify a cytoplasmic lncRNA, LINC01485, which promotes tumor growth and migration in gastric cancer. Materials and Methods: Microarray and computational analysis were utilized to identify differential expression in LINC01485 and EGFR. Real-time PCR and Western blotting assays were used to confirm the expression of LINC01485 and EGFR in gastric cancer cells. Cell proliferation, wound-healing and transwell assays were performed to measure cell growth, migration and invasion. Immunoprecipitation, RNA pull-down, and RNA fluorescence in situ hybridization (RNA-FISH) assays were used to test the interaction of c-Cbl with LINC01485 and EGFR. Furthermore, tumor xenograft in nude mice was performed to test tumor growth in vivo. Results: LINC01485 was upregulated and associated with tumor size, lymphatic metastasis and advanced pathological stage in gastric cancer. LINC01485 promoted gastric cancer cell proliferation, migration and invasion in vitro and in vivo. Furthermore, LINC01485 levels were positively correlated with EGFR expression in gastric cancer tissues and significantly increased the expression and phosphorylation (Tyr1045) of EGFR in gastric cancer cells. Mechanistically, LINC01485 competes with c-Cbl for binding to phosphorylated Tyr1045 site of EGFR, thus interfering with c-Cbl-mediated ubiquitination and subsequent degradation of EGFR. Conclusion: LINC01485 promoted EGFR stabilization and activation of EGFR/Akt signaling in gastric cancer. Our findings illustrate the diversity of cytoplasmic lncRNAs in signal transduction and highlight the important roles of lncRNAs in gastric cancer. [ABSTRACT FROM AUTHOR]

Background: Expanding opportunities to experience engaging STEM educational programs is an important pathway to increasing students' interest and competencies in STEM and, ultimately, motivation to pursue STEM careers. After-school programs offer one means to achieve this aim, but barriers such as a lack of transportation or available teachers may limit participation for some students in this context. Transitioning after-school STEM programs to in-school can provide opportunities to increase reach by removing these and other barriers. However, it is likely that this change in the learning context, from after-school to in-school, impacts student experiences and, ultimately, program efficacy by altering how students and teachers interact; as teachers and students adjust their behaviors and expectations to a more traditional learning context. To examine this potential effect, self-determination theory was used to frame how the learning context influences the social and motivational outcomes of a STEM program for underserved youth. In-school (N = 244; 39% girls, Mage = 13, 63% Caucasian, 18% African American, 6% Multiracial) and after-school (N = 70, 33% girls, Mage = 12, 55% Caucasian, 16% Multiracial, 13% Latino/a) program students completed surveys that assessed teacher-student interactions, and student psychological needs and motivation. In a structural equation model, student perceptions of teachers were entered as predictors of motivation for the program directly and mediated by psychological need satisfaction. Learning context (0 = in-school, 1 = after-school) was entered as a ubiquitous predictor. Results: Findings support the theorized model where perceptions of teachers positively predicted psychological need satisfaction (R2 =.20), and both variables positively predicted more self-determined motivation (R2 =.30–.35). Findings also demonstrate an effect of learning context where learning context negatively predicted the less self-determined motivations only (R2 =.06–.10) (i.e., in-school contexts are associated with less desirable motivational outcomes). Conclusion: Findings reinforce the instrumental role of students' positive perceptions of teachers in fostering a more desirable self-determined motivation for STEM program participation. Additionally, in-school programs must consider and integrate novel approaches that mitigate the negative impact of established in-school structures and processes (e.g., grades and mandatory participation) on student motivation for these programs and, potentially, interest in STEM careers. [ABSTRACT FROM AUTHOR]

Background: Epithelial ovarian cancer (EOC) is one of the most prevalent malignancies affecting females worldwide; however, its etiology mechanism remains unclear. In various malignancies, miR-145-5p is a widely accepted and versatile miRNA. Therefore, our research focused on exploring the activity and etiology of miR-145-5p in the modulation of metastasis, migration, and proliferation of EOC cells. The direct reactions between the 3′UTRs of SMAD4 mRNA and miR-145-5p were verified using dual luciferase reporter test. SKOV-3 cells were subsequently transfected using miR-145-5p mimics. Cell migration, death, and proliferation were evaluated using MTT, flow cytometry, and Transwell test. In addition, SMAD4 transcription and translation were evaluated using qRT-PCR and Western blot. Results: We found that miR-145-5p expression was repressed prevalently in EOC tissues, apart from SMAD4 upregulation. Excessive miR-145-5p expression remarkably reinforced EOC cell death and repressed EOC cell proliferation. Furthermore, upregulated miR-145-5p expression noticeably repressed migration via MMP-2 and MMP-9 downregulation. Moreover, SMAD4 was downregulated via miR-145-5p transfection. The dual luciferase test revealed that miR-145-5p directly targeted SMAD4. Conclusions: Our research suggests that miR-145-5p serves as a malignancy repressor and exerts an essential impact on inhibiting malignancy generation and reinforcing EOC death via targeting SMAD4. MiR-145-5p application could serve as a promising strategy to treat EOC. [ABSTRACT FROM AUTHOR]

As a highly potent and highly selective oral inhibitor of FLT3/AXL, gilteritinib showed activity against FLT3D835 and FLT3‐ITD mutations in pre‐clinical testing, although its role on colorectal cancer (CRC) cells is not yet fully elucidated. We examined the activity of gilteritinib in suppressing growth of CRC and its enhancing effect on other drugs used in chemotherapy. In this study, we observed that, regardless of p53 status, treatment using gilteritinib induces PUMA in CRC cells via the NF‐κB pathway after inhibition of AKT and activation of glycogen synthase kinase 3β (GSK‐3β). PUMA was observed to be vital for apoptosis in CRC cells through treatment of gilteritinib. Moreover, enhancing induction of PUMA through different pathways could mediate chemosensitization by using gilteritinib. Furthermore, PUMA deficiency revoked the antitumour role of gilteritinib in vivo. Thus, our results indicate that PUMA mediates the antitumour activity of gilteritinib in CRC cells. These observations are critical for the therapeutic role of gilteritinib in CRC. [ABSTRACT FROM AUTHOR]

This paper studies the global stabilization problem for a class of uncertain time-delay nonlinear systems by designing a sampled-data output feedback controller via network. Under a lower triangular linear growth condition, when only the output is measurable, a sampled-data output feedback network controller, whose observer and control law are both linear, is constructed to solve the stabilization problem. Using a feedback domination design approach which substantially differs from the separation principle, we explicitly construct a Lyapunov–Krasovskill functional to prove the global asymptotic stability with the help of inductive proof method. The control law is discrete-time and linear, hence simulation examples be easily implemented with computers to show the effectiveness of our proposed method. [ABSTRACT FROM AUTHOR]

The determination of the equipment parameters of the shore-based power supply system depends on the short-circuit current of the system. In this paper, the low-pressure ship is taken as an example. According to the ship industry standard and national standard, the initial parameters of the system equipment are determined. The system short-circuit current is respectively calculated and compared by the standard value method and the equivalent voltage source method. Then the Power System Computer Aided Design ( PSCAD) software is used to build the system model, and the simulation analysis is carried out to verify the correctness of the calculation results. Finally, according to the calculation results, parameters are selected for primary equipment such as cables, switch cabinets, circuit breakers, and transformers. [ABSTRACT FROM AUTHOR]

MicroRNA-10b (miR-10b) has been reported to be specifically upregulated in glioma tissues and cell lines. The aim of the present study was to investigate the effect of miR-10b-5p on the proliferation and invasion of glioma cells, and the possible underlying molecular mechanism. Cell viability was evaluated using an MTT assay, and flow cytometry was performed for cell cycle analysis. The effects of miR-10b-5p on glioma cell migration and invasion were assessed using wound healing and Transwell assays, respectively. The activity of matrix metalloproteinase 2 (MMP2) was also determined using zymography. Furthermore, homeobox B3 (HOXB3) mRNA expression in glioma cells was examined using quantitative polymerase chain reaction analysis. The protein expression levels of HOXB3, high mobility group box 1 (HMGB1) and Ras homolog family member C (RhoC) were further measured using western blotting. It was observed that glioma cells transfected with miR-10b-5p inhibitor exhibited significantly decreased proliferation. The wound healing and Transwell assays demonstrated that the miR-10b-5p inhibitor reduced the ability of glioma cells to migrate and invade, while transfection with miR-10b-5p mimic exhibited the opposite effect. HOXB3 was downregulated by miR-10b-5p at both the mRNA and protein levels. In addition, the expression of proteins associated with migration and invasion, including HMGB1, RhoC and MMP2, was upregulated in glioma cells transfected with miR-10b-5p mimic, while these proteins were downregulated in cells transfected with miR-10b-5p inhibitor. Taken together, the findings of the present study indicated that miR-10b-5p downregulation suppressed glioma cell proliferation and invasion, possibly by modulating HOXB3, which may provide a novel bio-target for glioma therapy. [ABSTRACT FROM AUTHOR]

Human Endogenous Retrovirus type K (HERV-K) is the only HERV known to be insertionally polymorphic; not all individuals have a retrovirus at a specific genomic location. It is possible that HERV-Ks contribute to human disease because people differ in both number and genomic location of these retroviruses. Indeed viral transcripts, proteins, and antibody against HERV-K are detected in cancers, auto-immune, and neurodegenerative diseases. However, attempts to link a polymorphic HERV-K with any disease have been frustrated in part because population prevalence of HERV-K provirus at each polymorphic site is lacking and it is challenging to identify closely related elements such as HERV-K from short read sequence data. We present an integrated and computationally robust approach that uses whole genome short read data to determine the occupation status at all sites reported to contain a HERV-K provirus. Our method estimates the proportion of fixed length genomic sequence (k-mers) from whole genome sequence data matching a reference set of k-mers unique to each HERV-K locus and applies mixture model-based clustering of these values to account for low depth sequence data. Our analysis of 1000 Genomes Project Data (KGP) reveals numerous differences among the five KGP super-populations in the prevalence of individual and co-occurring HERV-K proviruses; we provide a visualization tool to easily depict the proportion of the KGP populations with any combination of polymorphic HERV-K provirus. Further, because HERV-K is insertionally polymorphic, the genome burden of known polymorphic HERV-K is variable in humans; this burden is lowest in East Asian (EAS) individuals. Our study identifies population-specific sequence variation for HERV-K proviruses at several loci. We expect these resources will advance research on HERV-K contributions to human diseases. [ABSTRACT FROM AUTHOR]

Tissue inhibitor of metalloproteinases (TIMPs) are endogenous inhibitors of matrix metalloproteinases that are involved in normal cellular processes and in the development and progression of atherosclerosis. Our purpose was to evaluate the polymorphisms of the TIMP-3 genes for their associations with carotid plaques or with serum protein levels in the Han Chinese population. Two promoter variants, −915A/G (rs2234921) and −1296T/C (rs9619311), were genotyped in 548 subjects with no plaques, 462 subjects with echogenic plaques, and 427 subjects with mixture plaques. The serum TIMP-3 levels were measured using an enzyme-linked immunosorbent assay (ELISA). There was a strong linkage disequilibrium between −1296T/C and −915A/G (D′ = 1.0, r2 = 0.991). The individuals with the genotype (TC+CC) were 1.8 times more likely to have mixture plaques than the individuals with the TT genotype (P = 0.001, OR: 1.836, 95%CI: 1.269-2.665). The frequency of the C allele in the mixture plaque group was significantly higher than in the no plaque group (P = 0.009, CI: 1.119-2.187). We observed a significant elevation of the TIMP-3 levels in the serum of patients affected with mixture plaques compared to those with no plaques (P = 0.013). The current data suggest that genetic variation in the TIMP-3 genes may contribute to individual differences in mixture plaque susceptibility in the Han Chinese population. [ABSTRACT FROM AUTHOR]

The polarization ratio method is used for fast in-situ characterization of unimodal condensed aerosols of e-cigarettes. The method is based on 90°-light scattering of polarized 680 nm laser light by the droplet ensemble inside an optically defined measuring volume. Mass median droplet diameter (MMD) is derived from the ratio of scattered light from horizontally and vertically polarized incident light beams assuming a fixed value of the geometric standard deviation of the aerosol mass distribution. MMD is used to correct for the size dependence of the mass-based scattering signal of vertically polarized light to obtain the mass concentration if the sensor is calibrated once with an aerosol with a fixed MMD. The sensor uses commercially available aerosol photometers, and its application to e-cigarette aerosols was validated with an impactor for MMD and with a filter measurement for mass concentration. Good correlation (r2 > 0.97) for both parameters was observed. Application ranges are mass concentration range 0.5-50 mg/L, MMD 0.2-1.2 µm, 100 ms time resolution, and 0.2-3 L/min flow rate. The usefulness of this simple sensor for e-cigarette aerosol characterization is demonstrated by developing a scaling law between MMD and operating parameters of an e-cigarette, i.e., puff flow rate and mass concentration. Copyright © 2018 American Association for Aerosol Research [ABSTRACT FROM AUTHOR]

We reported high-performance organic light-emitting diodes (OLEDs) with a maximum efficiency of 42.3 lm W−1 and color rendering index of 92 by facilitating charge carrier transportation and controlling the recombination zone. In addition, we demonstrated that inserting a thin interlayer in emitting layer prevented energy transfer between blue and green emission layers, providing a relative chromatic-stability OLEDs. Furthermore, the device also exhibit a rather low correlated color temperature (2202 K), corresponding to an excellent warm WOLED, which could meet the special requirement of energy saving and environment protection. [ABSTRACT FROM AUTHOR]

This paper demonstrates the influence of temperature, exciton concentration, and electron transportation layers on the excimer emission of a novel deep-blue material: 4,4′-bis(4′′-triphenylsilyl) phenyl-1,1′-binaphthalene (SiBN), by studying the photoluminescence and electroluminescence spectra of SiBN-based film. We have further developed sunlight-like and warm-light white organic light-emitting diodes (WOLEDs) with high efficiency and wide-range spectra, using SiBN and bis(2-phenylbenzothiozolato- N,C2′)iridium(acetylacetonate) (btIr(acac)) as the blue excimer and yellow materials, respectively. The resulting device exhibited an excellent spectra overlap ratio of 82.9 % with sunlight, while the device peak current efficiency, external quantum efficiency, and power efficiency were 18.5 cd/A, 6.34 %, and 11.68 lm/W, respectively, for sunlight-like WOLEDs. [ABSTRACT FROM AUTHOR]

Two novel iridium(iii) complexes [Ir(ppy)2(PhOXD)] (1, ppy = 2-phenylpyridine, PhOXD = N-(5-phenyl-1,3,4-oxadiazol-2-yl)-benzamide) and [Ir(ppy)2(POXD)] (2, POXD = N-(5-phenyl-1,3,4-oxadiazol-2-yl)-diphenylphosphinic amide) have been designed and synthesized, and their photoluminescence and electrochemistry properties were investigated. At room temperature, complexes 1 and 2 show green emissions at about 502 and 506 nm with photoluminescence quantum yields (PLQYs) of 0.03 and 0.05 in CH2Cl2 solutions, respectively. In the 5 wt% doped poly(methyl methacrylate) (PMMA) film, the PLQYs (0.42 for complex 1, 0.52 for complex 2, respectively) increase significantly. The organic light emitting diodes (OLEDs) with the structure of ITO/HAT-CN (dipyrazino[2,3-f:2′,3′-h]quinoxaline-2,3,6,7,10,11-hexacarbonitrile), 10 nm)/TAPC (1,1-bis[4-(di-p-tolylamino)phenyl]cyclohexane, 40 nm)/Ir complexes (10 wt%): mCP (1,3-bis(9H-carbazol-9-yl)benzene, 20 nm)/TPBi (1,3,5-tris(1-phenyl-1H-benzimidazol-2-yl)benzene, 40 nm)/LiF (1 nm)/Al (100 nm) show good performances. In particular, device G2 based on complex 2 shows superior performances with a peak current efficiency (ηc) of 64.7 cd A−1 and a peak power efficiency (ηp) of 42.5 lm W−1 with low electroluminescence efficiency roll-off. The ηc value still remains over 60.6 cd A−1 even at a luminance of 10 000 cd m−2, which indicates that introducing electron transporting 1,3,4-oxadiazole and diphenyl phosphoryl groups into iridium complexes is an effective means of achieving efficient phosphors in OLEDs. [ABSTRACT FROM AUTHOR]

miR-25 was identified as an essential oncogene by promoting the growth and metastasis through TOB1 in gastric cancer (GC). The function of the single nucleotide polymorphism (SNP) located in the mature region of miR-25 (rs41274221) has not been investigated. In this study, we aimed to explore the involvement of rs41274221 in miR-25 in gastric cancer. We found that SNP rs41274221 in miR-25 was participated in the occurrence of GC by acting as a tumor protective factor associating with the tumor growth and metastasis. Besides, further investigation found that upregulation of miR-25 with AA genotype could attenuate the proliferation and invasion of tumor cells caused by wild-type miR-25. The dual-luciferase reporter assay also confirmed that miR-25 harbored the A allele which caused an incapacitation of binding at the TOB1. In conclusion, rs41274221 in miR-25 was a subgroup which may protect the patients from further growth and metastasis of gastric cancer and might serve as a novel biomarker for the disease. [ABSTRACT FROM AUTHOR]

Substantial individual differences characterize the changes induced by total sleep deprivation on cognitive functions. Despite some progress having been achieved, the mechanisms of individual differences in response to total sleep deprivation have not been clearly elucidated. Cerebral metabolism in the resting state is among the key physiological processes supporting the daily function of the brain, and may play an important role in these individual differences. Twenty-two right-handed participants (nine females and 13 males) between 20 and 26 years old completed a mathematical processing task both in resting wakefulness and after 24 h of total sleep deprivation. Fluorine-18 fluorodeoxyglucose positron emission tomography-computed tomography was used to investigate brain metabolism changes. The mathematical task was performed after the positron emission tomography scans were completed. Correlation analysis was used to investigate the correlations between cognitive performance changes and brain metabolism changes. Large inter-individual differences were found in the throughput changes, but these inter-individual differences were not associated with baseline or post-deprivation performance levels. Specifically, deterioration of throughput on the mathematical processing task was significantly correlated with metabolism changes in the superior frontal medial gyrus. These findings suggested that frontal metabolic activity contributes to individual differences in waking-induced impairment of cognitive performance. [ABSTRACT FROM AUTHOR]

Increasing evidence indicates that abnormal microRNA (miRNA) expression is related to hepatocellular carcinoma (HCC) development. Our study aimed to elucidate the essential role of miR-329 in HCC progression. Real-time PCR was used to analyze miR-329 and bromodomain containing 4 (BRD4) expression in HCC samples ( n = 135). Cell Counting Kit-8 (CCK-8) and flow cytometric analysis were used to investigate cell proliferation and apoptosis. The transwell assay was used to examine the cell invasive ability. The regulation mechanism was confirmed by luciferase reporter and western blot assays. Kaplan-Meier analysis was used to detect the function of miR-329 on the prognosis of HCC patients. miR-329 was decreased in HCC samples and was related to tumor development. Furthermore, miR-329 significantly regulated cell invasion by targeting BRD4 but had no effect on cell proliferation and apoptosis. Moreover, downregulation of miR-329 predicted poor prognosis of HCC patients. miR-329 could control cell invasion via regulating BRD4 expression and may be a prognostic marker in HCC. [ABSTRACT FROM AUTHOR]

Pinus massoniana bark proanthocyanidins (PMBPs), an active component isolated from Pinus massoniana bark, has been reported to possess a wide range of biochemical properties. Here, we investigated the anti-tumor effect of PMBPs on ovarian cancer. The results indicated that PMBPs significantly reduced the growth of ovarian cancer cells and induced dose-dependent apoptosis. The underlying mechanisms involved were elucidated to include the loss of mitochondrial membrane potential, down-regulation of the anti-apoptotic protein Bcl-2 and the activation of Caspase 3/9, suggesting that PMBPs triggered apoptosis through activation of mitochondria-associated apoptotic pathway. In addition, wound healing and transwell chamber assays revealed that PMBPs could suppress migration and invasion of ovarian cancer cells. PMBPs dramatically inhibited MMP-9 activity and expression, blocked the activity of NFκB and the activation of ERK1/2 and p38 MAPK. Our findings suggest that PMBPs has the potential to be developed as an anti-tumor drug for ovarian cancer treatment and/ or disease management. [ABSTRACT FROM AUTHOR]