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Affinity labeling of biomacromolecules is vital for bioimaging and functional studies. However, affinity probes recognizing glycans with high specificity remain scarce. Here we report the development of glycan recombinant affinity binders (GRABs) based on mutant bacterial sialidases, which are enzymatically inactive but preserve stringent specificity for sialoglycan substrates. By mutating a key catalytic residue of Streptococcus pneumoniae neuraminidase A (SpNanA) and Ruminococcus gnavus neuraminidase H (RgNanH), we develop GRAB-Sia and GRAB-Sia3 recognizing total sialoglycans and α2,3-sialosides, respectively. The GRABs exhibit strict substrate and linkage specificity, and tetramerization with streptavidin substantially increases their avidity. The GRABs and tetrameric GRABs (tetra-GRABs) are effective tools for probing sialoglycans in immunoblotting, flow cytometry, immunoprecipitation, and fluorescence imaging. Furthermore, multiplex analysis with tetra-GRABs uncovers spatially distinct sialoglycans in the various mouse organs. This work provides a versatile toolkit for labeling and analyzing sialoglycans with high specificity, sensitivity, and convenience. Highly specific glycan labels remain scarce, limiting their use in many applications. Here, authors report recombinant glycan affinity binders based on sialidase mutants, which are enzymatically inactive but preserve specificity for sialoglycans. [ABSTRACT FROM AUTHOR]

Carbon dioxide (CO2) electroreduction has extraordinary significance in curbing CO2 emissions while simultaneously producing value-added chemicals with economic and environmental benefits. In recent years, breakthroughs in designing catalysts, optimizing intrinsic activity, developing reactors, and elucidating reaction mechanisms have continuously driven the advancement of CO2 electroreduction. However, the industrialization of CO2 electroreduction remains a challenging task, with high energy consumption, high costs, limited reaction products, and restricted application scenarios being the issues that urgently need to be addressed. To accelerate the progress of CO2 electroreduction towards practical application, this review shifts the research focus from catalysts to aspects such as reactions and systems, aiming to improve reaction efficiency, reduce technical costs, expand the range of products, and enhance selectivity, offering readers a new perspective. In particular, innovative and specific design strategies such as CO2 reduction coupled with alternative oxidation, co-reduction reaction of CO2 and C/N/O/S-containing species, cascade systems, and integrated CO2 capture and reduction systems are discussed in detail. Additionally, personal views on the opportunities and future challenges of the aforementioned innovative strategies are provided, offering new insights for the future research and development of CO2 electroreduction. [ABSTRACT FROM AUTHOR]

Background: Cerebrospinal fluid (CSF) β2-microglobulin (β2M) has been demonstrated as an important factor in β-amyloid (Aβ) neurotoxicity and a potential target for Alzheimer’s disease (AD). However, more investigation is required to ascertain the relationship between β2M and glial activities in AD pathogenesis. Methods: In this study, 211 participants from the Alzheimer’s disease Neuroimaging Initiative (ADNI) with CSF and Plasma β2M, CSF glial fibrillary acidic protein (GFAP), soluble triggering receptor expressed on myeloid cells 2 (sTREM2), Aβ42, phosphorylated-tau (P-tau) and total tau (T-tau) were divided into four groups, stage 0, 1, 2, and suspected non-AD pathology (SNAP) based on the National Institute on Aging- Alzheimer’s Association (NIA-AA) criteria. Multiple linear regression, linear mixed effects models, and causal mediation analyses bootstrapped 10,000 iterations were used to investigate the underlying associations among β2M and CSF biomarkers at baseline and during a longitudinal visit. Results: CSF β2M concentration decreased with amyloid in stage 1 compared with stage 0 and increased with tau pathology and neurodegeneration in stage 2 and SNAP compared with stage 1. Moreover, CSF β2M level was positively correlated with the Aβ42 (β = 0.230), P-tau (β = 0.564), T-tau (β = 0.603), GFAP (β = 0.552), and sTREM2 (β = 0.641) (all P < 0.001). CSF β2M was only longitudinally correlated with T-tau change. The correlation of CSF β2M with P-tau (proportion = 25.4%, P < 0.001) and T-tau (proportion = 26.7%, P < 0.001) was partially mediated by GFAP in total participants, reproduced in late-life individuals. Furthermore, the astrocyte cascade also partially mediated the pathological relationship between CSF β2M and tau pathology (β2M → GFAP → YKL-40 → P-tau/T-tau, IE: 0.424—0.435, all P < 0.001). Nevertheless, the mediation effects of sTREM2 were not significant. Additionally, there was no association between plasma β2M and CSF biomarkers. Conclusions: CSF β2M is dynamic in AD pathology and associated with neuroinflammation. CSF GFAP might mediate the association between β2M and tau pathology, complementing the existing research on the effect of β2M in AD pathology and providing a new perspective on treatment. [ABSTRACT FROM AUTHOR]

A Cu2O-derived catalyst selectively and durably electroreduces CO2 to formate with a maximum faradaic efficiency of 74% in S2−-containing electrolyte and exhibits a high formate partial current density of up to 110 mA cm−2 in a flow cell. [ABSTRACT FROM AUTHOR]

Background: Scientific research activity in hospitals is important for promoting the development of clinical medicine, and the scientific literacy of medical staff plays an important role in improving the quality and competitiveness of hospital research. To date, no index system applicable to the scientific literacy of medical staff in China has been developed that can effectively evaluate and guide scientific literacy. This study aimed to establish an index system for the scientific literacy of medical staff in China and provide a reference for improving the evaluation of this system. Methods: In this study, a preliminary indicator pool for the scientific literacy of medical staff was constructed through the nominal group technique (n = 16) with medical staff. Then, two rounds of Delphi expert consultation surveys (n = 20) were conducted with clinicians, and the indicators were screened, revised and supplemented using the boundary value method and expert opinions. Next, the hierarchical analysis method was utilized to determine the weights of the indicators and ultimately establish a scientific literacy indicator system for medical staff. Results: Following expert opinion, the index system for the scientific literacy of medical staff featuring 2 first-level indicators, 9 second-level indicators, and 38 third-level indicators was ultimately established, and the weights of the indicators were calculated. The two first-level indicators were research literacy and research ability, and the second-level indicators were research attitude (0.375), ability to identify problems (0.2038), basic literacy (0.1250), ability to implement projects (0.0843), research output capacity (0.0747), professional capacity (0.0735), data-processing capacity (0.0239), thesis-writing skills (0.0217), and ability to use literature (0.0181). Conclusions: This study constructed a comprehensive scientific literacy index system that can assess medical staff's scientific literacy and serve as a reference for evaluating and improving their scientific literacy. [ABSTRACT FROM AUTHOR]

The applications of silica-based glass have evolved alongside human civilization for thousands of years. High-precision manufacturing of three-dimensional (3D) fused silica glass objects is required in various industries, ranging from everyday life to cutting-edge fields. Advanced 3D printing technologies have emerged as a potent tool for fabricating arbitrary glass objects with ultimate freedom and precision. Stereolithography and femtosecond laser direct writing respectively achieved their resolutions of ~50 μm and ~100 nm. However, fabricating glass structures with centimeter dimensions and sub-micron features remains challenging. Presented here, our study effectively bridges the gap through engineering suitable materials and utilizing one-photon micro-stereolithography (OμSL)-based 3D printing, which flexibly creates transparent and high-performance fused silica glass components with complex, 3D sub-micron architectures. Comprehensive characterizations confirm that the final material is stoichiometrically pure silica with high quality, defect-free morphology, and excellent optical properties. Homogeneous volumetric shrinkage further facilitates the smallest voxel, reducing the size from 2.0 × 2.0 × 1.0 μm3 to 0.8 × 0.8 × 0.5 μm3. This approach can be used to produce fused silica glass components with various 3D geometries featuring sub-micron details and millimetric dimensions. This showcases promising prospects in diverse fields, including micro-optics, microfluidics, mechanical metamaterials, and engineered surfaces. 3D-printed glass holds great potential. However, it is challenging to control both the dimension and the resolution of the printed material. Here, authors present a one-photon 3D printing approach to produce high-performance fused silica glass with sub-micron resolution and millimetric dimensions. [ABSTRACT FROM AUTHOR]

The instability of the river channels has increased in response to the combined effects of global warming and human activity. This instability threatens the lives and property of people who live along river courses. This study takes the Pingluo section of the upper Yellow River, which is ~28 km long and ~400 m wide, as its research focus. We studied 11 periods of Landsat remote sensing images from 1973 to 2023 and analyzed the evolutionary characteristics of the Pingluo section over the past 50 years. The channel morphology indices included the channel braiding index (BIT), the bar braiding index (BIB), the average area of the bar (Ab), and the width of the wet channel area (BW). The results showed that there was an overall shrinking trend in this section of the Yellow River; more particularly, fluctuations in indicators such as the river width and the braiding index highlighted an increasing–decreasing–increasing pattern of change. During the 1973–1986 period, the river showed a widening trend, with narrow anabranches cutting through the floodplain and both the river width and the braiding index increasing dramatically over a short period. Over the 1986–2018 period, the area of the wet channel continued to shrink to its lowest level for the past 50 years, the river's branches were diverted and abandoned, and the channel tended to straighten out. Between 2018 and 2023, the river showed a slightly expanding trend. The evolution of the river channel appears to be related to regional human activity and climate change. For example, after the joint filling of the Longyangxia and Liujiaxia reservoirs in the upper reaches of the Pingluo section of the Yellow River in 1986, runoff and sediment load along this section decreased, flood events became less frequent, and the channel tended to contract. In addition, the increase in extreme precipitation events over the last five years has led to an increase in the magnitude and frequency of peak discharge values in the region, which is the main reason for the increase in the river braiding index and area. [ABSTRACT FROM AUTHOR]

Electrochemical CO2 reduction to value‐added chemicals or fuels offers a promising approach to reduce carbon emissions and alleviate energy shortage. Cu‐based electrocatalysts have been widely reported as capable of reducing CO2 to produce a variety of multicarbon products (e.g. ethylene and ethanol). In this work, we develop sulfur‐doped Cu2O electrocatalysts, which instead can electrochemically reduce CO2 to almost exclusively formate. We show that a dynamic equilibrium of S exists at the Cu2O‐electrolyte interface, and S‐doped Cu2O undergoes in situ surface reconstruction to generate active S‐adsorbed metallic Cu sites during the CO2 reduction reaction (CO2RR). Density functional theory (DFT) calculations together with in situ infrared absorption spectroscopy measurements show that the S‐adsorbed metallic Cu surface can not only promote the formation of the *OCHO intermediate but also greatly suppress *H and *COOH adsorption, thus facilitating CO2‐to‐formate conversion during the electrochemical CO2RR. [ABSTRACT FROM AUTHOR]

Electrochemical CO2 reduction to value‐added chemicals or fuels offers a promising approach to reduce carbon emissions and alleviate energy shortage. Cu‐based electrocatalysts have been widely reported as capable of reducing CO2 to produce a variety of multicarbon products (e.g. ethylene and ethanol). In this work, we develop sulfur‐doped Cu2O electrocatalysts, which instead can electrochemically reduce CO2 to almost exclusively formate. We show that a dynamic equilibrium of S exists at the Cu2O‐electrolyte interface, and S‐doped Cu2O undergoes in situ surface reconstruction to generate active S‐adsorbed metallic Cu sites during the CO2 reduction reaction (CO2RR). Density functional theory (DFT) calculations together with in situ infrared absorption spectroscopy measurements show that the S‐adsorbed metallic Cu surface can not only promote the formation of the *OCHO intermediate but also greatly suppress *H and *COOH adsorption, thus facilitating CO2‐to‐formate conversion during the electrochemical CO2RR. [ABSTRACT FROM AUTHOR]

Purpose: The emergence of genomic targeted therapy has improved the prospects of treatment for breast cancer (BC). However, genetic testing relies on invasive and sophisticated procedures.Patients and Methods: Here, we performed ultrasound (US) and target sequencing to unravel the possible association between US radiomics features and somatic mutations in TNBC (n=83) and non-TNBC (n=83) patients. Least absolute shrinkage and selection operator (Lasso) were utilized to perform radiomic feature selection. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was utilized to identify the signaling pathways associated with radiomic features.Results: Thirteen differently represented radiomic features were identified in TNBC and non-TNBC, including tumor shape, textual, and intensity features. The US radiomic–gene pairs were differently exhibited between TNBC and non-TNBC. Further investigation with KEGG verified radiomic–pathway (ie, JAK-STAT, MAPK, Ras, Wnt, microRNAs in cancer, PI3K-Akt) associations in TNBC and non-TNBC.Conclusion: The pivotal network provided the connections of US radiogenomic signature and target sequencing for non-invasive genetic assessment of precise BC treatment. [ABSTRACT FROM AUTHOR]

Queue wait is a prevalent daily experience, with most prior research documenting its negative consequences on consumer outcomes. Recent research has suggested otherwise, revealing potential positive effects of queue wait. However, studies on the positive queue effects are rather limited and have largely neglected the role of consumers' affective experience in driving potential favorable consumer outcomes. Expanding this stream of research, in this article, we build on the queue wait literature and the curiosity literature to propose that the presence of queue waits generates feelings of curiosity and more importantly such curious feelings influence consumers' decision‐making in fashions that enhance their purchase intentions. The boundary condition for this effect was also examined. Five studies provided empirical evidence: the presence versus absence of queue wait enhances participants' purchase intentions that carry real consequences (Study 1); this effect is mediated by participants' feelings of curiosity after accounting for quality (Study 2) or after controlling for quality (Studies 1, 3–5), and is stronger among participants with hedonic versus utilitarian consumption motivation (Studies 4 and 5). By documenting the curiosity account for the positive effect of queue wait on consumers' purchase intentions, this research adds to extant research on positive queue effects and expands the curiosity literature into the queue wait context. The observed moderating effect of hedonic versus utilitarian consumption motivation echoes with the curiosity account. Practical implications for marketers were discussed. [ABSTRACT FROM AUTHOR]

Self‐renewal and differentiation of embryonic stem cells (ESCs) are influenced by protein O‐linked β‐N‐acetylglucosamine (O‐GlcNAc) modification, but the underlying mechanism remains incompletely understood. Herein, we report the identification of 979 O‐GlcNAcylated proteins and 1340 modification sites in mouse ESCs (mESCs) by using a chemoproteomics method. In addition to OCT4 and SOX2, the third core pluripotency transcription factor (PTF) NANOG was found to be modified and functionally regulated by O‐GlcNAc. Upon differentiation along the neuronal lineage, the O‐GlcNAc stoichiometry at 123 sites of 83 proteins—several of which were PTFs—was found to decline. Transcriptomic profiling reveals 2456 differentially expressed genes responsive to OGT inhibition during differentiation, of which 901 are target genes of core PTFs. By acting on the core PTF network, suppression of O‐GlcNAcylation upregulates neuron‐related genes, thus contributing to mESC fate determination. [ABSTRACT FROM AUTHOR]

Self‐renewal and differentiation of embryonic stem cells (ESCs) are influenced by protein O‐linked β‐N‐acetylglucosamine (O‐GlcNAc) modification, but the underlying mechanism remains incompletely understood. Herein, we report the identification of 979 O‐GlcNAcylated proteins and 1340 modification sites in mouse ESCs (mESCs) by using a chemoproteomics method. In addition to OCT4 and SOX2, the third core pluripotency transcription factor (PTF) NANOG was found to be modified and functionally regulated by O‐GlcNAc. Upon differentiation along the neuronal lineage, the O‐GlcNAc stoichiometry at 123 sites of 83 proteins—several of which were PTFs—was found to decline. Transcriptomic profiling reveals 2456 differentially expressed genes responsive to OGT inhibition during differentiation, of which 901 are target genes of core PTFs. By acting on the core PTF network, suppression of O‐GlcNAcylation upregulates neuron‐related genes, thus contributing to mESC fate determination. [ABSTRACT FROM AUTHOR]

Logistics distribution vehicle planning is an important issue in logistics transportation activities, and it is also a research hotspot in theoretical circles at home and abroad. At present, many studies have focused on establishing vehicle planning models and optimizing vehicle planning in different environments and have achieved rich results. As an important part of transportation production process, the efficiency of logistics distribution is very important to the whole production process. Especially for emergency logistics, every minute is very critical for emergency situations such as disaster relief. In order to improve the efficiency of emergency logistics, this paper applies multiagent technology to emergency logistics and puts forward an integrated modeling method of enterprise macromodeling, business process mesomodeling, and micromodel design. Using the agent-oriented system development method, an emergency logistics distribution vehicle planning model system is established. The development process of multiagent automatic trading system is described. The results show that it is feasible and effective to use multi-intelligent fuselage technology for emergency logistics distribution vehicle planning and decision-making. The algorithm proposed in this paper has advantages over the container order sequence processing scheme, and the total cost of order acceptance decreases sharply in the initial stage, which shows the practical convergence of the algorithm. The adjacency search method and Tabu search method deal with the calculation of total labor cost, and the Tabu neighborhood search algorithm obtains better results with lower labor cost. [ABSTRACT FROM AUTHOR]

Electrochemical CO2 reduction to value‐added chemicals/fuels provides a promising way to mitigate CO2 emission and alleviate energy shortage. CO2‐to‐CO conversion involves only two‐electron/proton transfer and thus is kinetically fast. Among the various developed CO2‐to‐CO reduction electrocatalysts, transition metal/N‐doped carbon (M‐N‐C) catalysts are attractive due to their low cost and high activity. In this work, recent progress on the development of M‐N‐C catalysts for electrochemical CO2‐to‐CO conversion is reviewed in detail. The regulation of the active sites in M‐N‐C catalysts and their related adjustable electrocatalytic CO2 reduction performance is discussed. A visual performance comparison of M‐N‐C catalysts for CO2 reduction reaction (CO2RR) reported over the recent years is given, which suggests that Ni and Fe‐N‐C catalysts are the most promising candidates for large‐scale reduction of CO2 to produce CO. Finally, outlooks and challenges are proposed for future research of CO2‐to‐CO conversion. [ABSTRACT FROM AUTHOR]

It is widely accepted that single Ni atoms coordinated with N can highly efficiently promote CO2 electroreduction to CO. Very recently, probably due to limited access to high‐angle annular dark‐field scanning transmission electron microscopy (HAADF‐STEM) techniques, a misleading conclusion that nitrogen‐doped carbon‐encapsulated Ni nanoparticles (NPs) possess activity similar to that of single Ni atoms was reported and was quickly followed by several similar reports. The current contribution aims to end this misleading conclusion by performing well‐designed experiments and solid theoretical analyses. For this purpose, a series of Ni/nitrogen‐doped graphite (Ni‐NG) catalysts with different dominant Ni species (single Ni atom, nitrogen‐doped carbon‐encapsulated Ni NPs, or both) are fabricated and comparatively studied for CO2 electroreduction to CO. Two previous studies that reported nitrogen‐doped carbon‐encapsulated Ni NPs catalysts are reinvestigated, and the existence of single Ni atoms is confirmed by HAADF‐STEM. In addition, after leaching out most of the Ni NPs by a strong acid, the activity of those catalysts does not decrease but increases slightly. Density functional theory results suggest that nitrogen‐doped carbon‐encapsulated Ni NPs are highly selective for the hydrogen evolution reaction (HER) rather than for CO2‐to‐CO conversion. Overall, both systematic experimental and theoretical analyses clearly reveal that the nitrogen‐doped carbon‐encapsulated Ni NPs are not active for CO2 electroreduction. [ABSTRACT FROM AUTHOR]

In this study, for the first time, we report a straightforward one-pot method to obtain ZnS with multiple vacancies (V+V−–ZnS). It is found that two new defect energy levels are formed by Zn vacancies and S vacancies, which are close to the valence band (VB) top and conduction band (CB) bottom, respectively. The newly formed energy levels in V+V−–ZnS greatly enhance light absorption: 480 nm and 2.44 eV (V+V−–ZnS) vs. 399 nm and 3.24 eV (ZnS). Moreover, V+V−–ZnS exhibits an evidently improved charge separation efficiency. Thus, V+V−–ZnS shows a high visible-light activity, while ZnS has no response to visible light. This method is simple, and could be extended to mass production. [ABSTRACT FROM AUTHOR]

Dramatically increased CO2 concentration from several point sources is perceived to cause severe greenhouse effect towards the serious ongoing global warming with associated climate destabilization, inducing undesirable natural calamities, melting of glaciers, and extreme weather patterns. CO2 capture and utilization (CCU) has received tremendous attention due to its significant role in intensifying global warming. Considering the lack of a timely review on the state-of-the-art progress of promising CCU techniques, developing an appropriate and prompt summary of such advanced techniques with a comprehensive understanding is necessary. Thus, it is imperative to provide a timely review, given the fast growth of sophisticated CO2 capture and utilization materials and their implementation. In this work, we critically summarized and comprehensively reviewed the characteristics and performance of both liquid and solid CO2 adsorbents with possible schemes for the improvement of their CO2 capture ability and advances in CO2 utilization. Their industrial applications in pre- and post-combustion CO2 capture as well as utilization were systematically discussed and compared. With our great effort, this review would be of significant importance for academic researchers for obtaining an overall understanding of the current developments and future trends of CCU. This work is bound to benefit researchers in fields relating to CCU and facilitate the progress of significant breakthroughs in both fundamental research and commercial applications to deliver perspective views for future scientific and industrial advances in CCU. [ABSTRACT FROM AUTHOR]

Reactive species, such as.PO2 and HOPO, are considered of upmost importance in flame inhibition and catalytic combustion processes of fuels. However, the underlying chemistry of their formation remains speculative due to the unavailability of suitable analytical techniques that can be used to identify the transient species which lead to their formation. This study elucidates the reaction mechanisms of the formation of phosphoryl species from dimethyl methyl phosphonate (DMMP) and dimethyl methyl phosphoramidate (DMPR) under well‐defined oxidative conditions. Photoelectron photoion coincidence techniques that utilized vacuum ultraviolet synchrotron radiation were applied to isomer‐selectively detect the elusive key intermediates and stable products. With the help of in situ recorded spectral fingerprints, different transient species, such as PO2 and triplet O radicals, have been exclusively identified from their isomeric components, which has helped to piece together the formation mechanisms of phosphoryl species under various conditions. It was found that.PO2 formation required oxidative conditions above 1070 K. The combined presence of O2 and H2 led to significant changes in the decomposition chemistry of both model phosphorus compounds, leading to the formation of.PO2. The reaction.PO+O2→.PO2+O: was identified as the key step in the formation of.PO2. Interestingly, the presence of O2 in DMPR thermolysis suppresses the formation of PN‐containing species. In a previous study, PN species were identified as the major species formed during the pyrolysis of DMPR. Thus, the findings of this study has shed light onto the decomposition pathways of organophosphorus compounds, which are beneficial for their fuel additive and fire suppressant applications. [ABSTRACT FROM AUTHOR]

Flexible and foldable microcomponents are highly desirable for energy devices. In this work, flexible asymmetric micro‐capacitor (AMC) is fabricated by using uniform Ag/Bi2S3 (or Bi2S3) nanoflowers, active carbon and carbon fibres are used as positive material, negative material and current collector, respectively. On one hand, the areal capacitance of Ag/Bi2S3 electrode has increased by more than 6 times than Bi2S3, and 86 and 68% of capacitances are retained for Ag/Bi2S3 and Bi2S3 electrodes after 4000 cycles, respectively. On the other hand, Ag/Bi2S3‐based AMC has an energy density of (0.0134 mWhcm−3) 4.36 times higher than Bi2S3‐based one (0.0025 mWhcm−3); after 4000 cycles, 84 and 33% of capacitances are retained for Ag/Bi2S3‐ and Bi2S3‐based AMCs, respectively. The improved performances are mainly attributed to the enhanced electrical conductance by Ag loading (Bi2S3: 21.898 Ω; Ag/Bi2S3: 4.5643 Ω). Most importantly, the AMC is flexible and foldable, which is promising to conveniently apply in complicated energy devices, production for practical applications. [ABSTRACT FROM AUTHOR]

In this work, α-MoO3 and h-MoO3 are synthesized by a simple hydrothermal method. Under ambient conditions, α-MoO3 has a high catalytic wet air oxidation (CWAO) activity for methylene blue (MB), while h-MoO3 has no activity: 64% of MB can be degraded after 20 min. When adding an additional man-made light source, the performances of h-MoO3 and α-MoO3 remain almost unchanged. However, with elevating the reaction temperature from 25 to 40 °C, the activity has not been improved for h-MoO3, while the activity of α-MoO3 also has no improvement from 25 to 35 °C. When the temperature rises to 40 °C, the performance of α-MoO3 improved significantly: the degradation percentage reaches 90% after 20 min. Crystal structure analysis results show that the activity of α-MoO3 originates from Jahn–Teller distortion of the [MoO6] octahedron, resulting in the formation of Lewis acid sites. Furthermore, H2-temperature programmed reduction (H2-TPR) and O2-temperature programmed desorption (O2-TPD) results indicate that α-MoO3 has a higher lattice oxygen mobility and a higher oxidization ability than h-MoO3. This ambient CWAO process is energy-saving and low-cost, which is promising for indoor clearance. [ABSTRACT FROM AUTHOR]

Macrophage polarization serves an important role in immune regulation that is regulated by T-cell immunoglobulin-mucin-3 (Tim-3). The objective of the present study was to explore the role of 1,25-dihydroxy-vitamin D3 [1,25(OH)2D3] in macrophage polarization. Plasmid transfection techniques were applied to prepare RAW264.7 cells with silenced or overexpressed Tim-3 gene. ELISAs were used to examine the level of inflammatory factors secreted by macrophages. Proteins levels were determined by western blot analysis. mRNAs expression levels were assessed using reverse transcription quantitative polymerase chain reaction. It was identified that 1,25(OH)2D3 upregulated Tim-3 levels and promoted the secretion of interleukin (IL)-10. 1,25(OH)2D3 was also observed to increase the level of transforming growth factor-β and to inhibit tumor necrosis factor-α and IL-6. The results also suggested that Tim-3 gene silencing induced macrophages polarization to classically activated macrophages (M1), and that overexpression of the Tim-3 gene induced macrophage polarization to alternatively activated macrophages (M2). 1,25(OH)2D3 treatment upregulated the expression level of Tim-3 in macrophages, which promoted cell polarization to M2 and inhibited polarization to M1. The data from the present study indicated that Tim-3 may induce macrophage polarization to M2, and that 1,25(OH)2D3 produced immunosuppressive effects by upregulating Tim-3. [ABSTRACT FROM AUTHOR]

The thermal behavior of organophosphorus compounds is intricate and poorly understood but crucial for understanding gas-phase flame inhibition, syntheses of thermally active phosphorus-based reactive precursors, catalytic combustion, incineration of toxic nerve gases, and astrochemistry. In this work, the pyrolysis of dimethyl phosphoramidate was investigated using photoion photoelectron coincidence spectroscopy in combination with vacuum ultraviolet synchrotron radiation. This technique enables isomer-selective detection of reactive intermediates, which are crucial in the understanding of the decomposition process. Combined with quantum chemical calculations, the experimental results permit the formulation of a comprehensive pyrolysis reaction pathway for dimethyl phosphoramidate, consisting of several reactive phosphorus species on four possible decomposition pathways. Compared to the decomposition of dimethyl methyl phosphonate, which leads exclusively to the formation of PO radicals, substitution of the methyl with an amino group most notably yields phosphorus nitride (P≡N). This mostly favored reaction pathway involves the subsequent loss of methanol and formaldehyde to yield three PONH2 tautomers, which eliminate water to generate P≡N. The thermally induced production of PN species and its possible role in flame inhibition has not previously been reported. In addition, the adiabatic ionization energy of O=P(OCH3)2NH2 was determined to be 9.79±0.02 eV. [ABSTRACT FROM AUTHOR]

The production of phosphoryl species (PO, PO2, HOPO) is believed to be of great importance for efficient flame-retardant action in the gas phase. We present a detailed investigation of the thermal decomposition of dimethyl methylphosphonate (DMMP) probed by vacuum ultraviolet (VUV) synchrotron radiation and imaging photoelectron photoion coincidence (iPEPICO) spectroscopy. This technique provides a snapshot of the thermolysis process and direct evidence of how the reactive phosphoryl species are generated during heat exposure. One of the key findings of this work is that only PO is formed in high concentration upon DMMP decomposition, whereas PO2 is absent. It can be concluded that the formation of PO2 needs an oxidative environment, which is typically the case in a real flame. Based on the identification of products such as methanol, formaldehyde, and PO, as well as the intermediates OPCH3, H2CPOH, and H2CP(O)H, supported by quantum chemical calculations, we were able to describe the predominant pathways that lead to active phosphoryl species during the thermal decomposition of DMMP. [ABSTRACT FROM AUTHOR]