Search

The walnut (Juglans regia L.) is a typical and an economically important tree species for nut production with heterodichogamy. The absence of female and male flowering periods seriously affects both the pollination and fruit setting rates of walnuts, thereby affecting the yield and quality. Therefore, studying the characteristics and processes of flower bud differentiation helps in gaining a deeper understanding of the regularity of the mechanism of heterodichogamy in walnuts. In this study, a total of 3540 proteins were detected in walnut and 885 unique differentially expressed proteins (DEPs) were identified using the isobaric tags for the relative and absolute quantitation (iTRAQ)-labeling method. Among all DEPs, 12 common proteins were detected in all four of the obtained contrasts. GO and KEGG analyses of 12 common DEPs showed that their functions are distributed in the cytoplasm metabolic pathways, photosynthesis, glyoxylate and dicarboxylate metabolism, and the biosynthesis of secondary metabolites, which are involved in energy production and conversion, synthesis, and the breakdown of proteomes. In addition, a function analysis was performed, whereby the DEPs were classified as involved in photosynthesis, morphogenesis, metabolism, or the stress response. A total of eight proteins were identified as associated with the morphogenesis of stamen development, such as stamen-specific protein FIL1-like (XP_018830780.1), putative leucine-rich repeat receptor-like serine/threonine-protein kinase At2g24130 (XP_018822513.1), cytochrome P450 704B1-like isoform X2 (XP_018845266.1), ervatamin-B-like (XP_018824181.1), probable glucan endo-1,3-beta-glucosidase A6 (XP_018844051.1), pathogenesis-related protein 5-like (XP_018835774.1), GDSL esterase/lipase At5g22810-like (XP_018833146.1), and fatty acyl-CoA reductase 2 (XP_018848853.1). Our results predict several crucial proteins and deepen the understanding of the biochemical mechanism that regulates the formation of male and female flower buds in walnuts. [ABSTRACT FROM AUTHOR]

Grape white rot is a devastating fungal disease caused by Coniella diplodiella. The pathogen delivers effectors into the host cell that target crucial immune components to facilitate its infection. Here, we examined a secreted effector of C. diplodiella , known as CdE1, which has been found to inhibit Bax-triggered cell death in Nicotiana benthamiana plants. The expression of CdE1 was induced at 12–48 h after inoculation with C. diplodiella , and the transient overexpression of CdE1 led to increased susceptibility of grapevine to the fungus. Subsequent experiments revealed an interaction between CdE1 and Vitis davidii cysteine-rich receptor-like kinase 10 (VdCRK10) and suppression of VdCRK10-mediated immunity against C. diplodiella , partially by decreasing the accumulation of VdCRK10 protein. Furthermore, our investigation revealed that CRK10 expression was significantly higher and was up-regulated in the resistant wild grapevine V. davidii during C. diplodiella infection. The activity of the VdCRK10 promoter is induced by C. diplodiella and is higher than that of Vitis vitifera VvCRK10 , indicating the involvement of transcriptional regulation in CRK10 gene expression. Taken together, our results highlight the potential of VdCRK10 as a resistant gene for enhancing white rot resistance in grapevine. [ABSTRACT FROM AUTHOR]

Fluorite and calcite have similar surface properties and natural floatability, so their flotation separation has always been a problem faced by the beneficiation industry. The key to flotation separation is the choice of depressants. Sodium hexametaphosphate (SHMP) has a good effect on fluorite calcite selective inhibition. In this paper, the effects of Zn2+ on the selective inhibition of SHMP in the flotation process of fluorite and calcite were studied through single mineral and artificial mixed mineral flotation experiments. Solution chemical calculation, X-ray photoelectron spectroscopy (XPS), Fourier Transform Infrared spectroscopy (FT-IR) and Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) analyses investigated the mechanism of action of Zn2+, which had the most significant negative effect on the selective inhibition of SHMP. The results show that the main components of SHMP inhibiting minerals are HPO42- and H2PO4-, which can react with Ca active sites on the mineral surface to form hydrophilic Ca(H2PO4)2 and CaHPO4, while Zn2+ The presence of HPO42- in solution resulted in the formation of stable ZnHPO4 complexes, thereby weakening the inhibitory effect of SHMP on minerals. [ABSTRACT FROM AUTHOR]

For industrial flotation foam image processing, accurate bubble size measurement and feature extraction are very important to optimize the flotation process and to improve the recovery of mineral resources. This paper presents an improved algorithm to investigate mineral flotation foam image segmentation for mineral processing. Several libraries implemented for the Python programming language are used for image enhancement and compensation, quantitative analysis of factors influencing the image segmentation accuracy, and suggestions for improvement of the flotation foam image processing. The bubble characteristics-size and morphology-and the influence of the flotation conditions on the flotation foam image are analyzed. A Python implementation of the Retinex image compensation method-region-adaptive and multiscale-is proposed to address known issues of uneven illumination and shadows affecting flotation foam images, thereby improving brightness uniformity. Finally, an improved version of the watershed segmentation algorithm included in the Python Open Source Computer Vision library is used for segmentation analysis. The accuracy of the flotation foam image segmentation is 3.3% higher than for the standard watershed algorithm and the segmentation time is 9.9% shorter. [ABSTRACT FROM AUTHOR]

This brief considers the Direction of Arrival (DOA) estimation for Distributed mmWave Radar System (DmmWRS) in the presence of coherent sources and a single snapshot. To this end, the large virtual aperture is firstly derived assuming that DmmWRS works in time-sharing mode. Then, a Fast Local Iterative Search Algorithm (FLISA) resorting to Iterative Adaptive Approach (IAA) and prior DOA information obtained by a single radar, is developed to accurately estimate DOA within a small azimuth region of interest. Finally, numerical simulations and actual measurements show the superiority of the proposed approach than some counterparts providing better angle measurement performance, more importantly, FLISA has lower computational complexity and faster convergence than IAA. [ABSTRACT FROM AUTHOR]

Grinding is an important link in the process of mineral processing. It plays a vital role in mineral processing by optimizing the grinding process, improving the quality of grinding products and ensuring the follow-up operation indicators. In this paper, the Python language, intelligent theoretical control technology and mineral processing were combined to solve the problem of ore feeding control in mineral processing. Using error factor analysis, an extended control algorithm was designed. The NumPy library and data collected from the Yuan YangMou concentrator in China were used to quantitatively analyze the factors affecting the error of electronic belt scales. This paper introduces the use of Kalman filtering for electronic belt scale weight data to reduce the effect of noise and hence reduce errors. The factors affecting the process of mill feeding are also analyzed. The core ideas and methods of fuzzy control theory are summarized, and a Python-based fuzzy controller suitable for the mill feeding process that improves the overall robustness and accuracy of feeding system is implemented. [ABSTRACT FROM AUTHOR]

Methyl jasmonate (MeJA) plays a vital role in plant disease resistance and also induces the expression of disease resistance genes in plants. In this study, a transcriptome analysis was performed on grapevine leaves after 12, 24 and 48 h of MeJA-100 μM treatment. A total of 1242 differentially expressed genes (DEGs) were identified from the transcriptome data, and the analysis of the DEGs showed that genes related to phytohormone signal transduction, jasmonic acid-mediated defense, Mitogen-activated protein kinase (MAPK), and flavonoid biosynthetic pathways were upregulated. As Pathogenesis-related gene 1 (PR1) is an important marker gene in plant defense also upregulated by MeJA treatment in RNA-seq data, the VvPR1 gene was selected for a promoter analysis with β-glucuronidase (GUS) through transient expression in tobacco leaves against abiotic stress. The results showed that the region from −1837 bp to −558 bp of the VvPR1 promoter is the key region in response to hormone and wound stress. In this study, we extended the available knowledge about induced defense by MeJA in a grapevine species that is susceptible to different diseases and identified the molecular mechanisms by which this defense might be mediated. [ABSTRACT FROM AUTHOR]

Video highlight detection (VHD) has emerged as a pressing task due to the unprecedentedly increasing amount of video data, such as those from e-commerce live-broadcasting platforms. Many approaches focus on exploiting text data, in the form of video description or time-sync comments, to facilitate the VHD task. Despite the promising results, they have largely overlooked the noises inherent in the text data and have mostly relied on isolating the feature of text and video. In this paper, we introduce a novel model to handle VHD, termed Coevolution Network (CoEvo-Net), that allows us to account for joint learning of the language and video features explicitly via a coevolution paradigm, in which features from the two data modalities progressively refine each other. This is achieved by a dedicated CoEvo-Cell that takes language and video together as inputs, extracts cross-modality, and filters the undesired parts of the input, such as words in a sentence. Furthermore, we release a large-scale dataset of e-commerce for VHD, in which each video is coupled with a sentence for description, to benchmark the sentence-based VHD approaches. Extensive experiments on the released dataset demonstrate that CoEvo-Net achieves state-of-the-art performance. Our dataset and code will be made publicly available. [ABSTRACT FROM AUTHOR]

Existing wideband beampattern synthesis methods usually require multiple beamformers, each corresponding to one frequency bin, which results in excessive resource consumption in engineering practice. To alleviate the resource consumption of wideband array beamforming, we propose a novel time-domain wideband digital beamformer that can control the beam shape over the whole bandwidth for interference mitigation by using only one set of amplitude–phase weights and integer time delay filters. To tackle the resultant optimization problem, two effective algorithms are introduced, and both can solve the problem within polynomial time. One is an alternating optimization algorithm that decomposes the original problem into multiple tractable subproblems iteratively. The other is the convex approximation algorithm that transforms the original problem into a series of second-order cone programming problems iteratively. The two algorithms exhibit their respective advantages in terms of convergence and computational complexity. Extensive numerical examples are presented to evaluate the proposed time-domain wideband beamforming strategy, highlighting that it can form a deep notch in any interested spatial–spectral region to suppress the strong interferences. [ABSTRACT FROM AUTHOR]

This letter considers a distributed cascade of two millimeter-wave (mmWave) radars to achieve a large virtual aperture for high-resolution angle measurement. In order to explore the high sidelobe problem caused by distributed radars, we derive the general Bartlett spatial spectrum expression of the virtual array. Furthermore, we introduce four cases to describe the relationship between the radar spacing, the number of transmitters/receivers, and the sidelobe position of the spatial spectrum. Finally, numerical simulation and experiment examples are provided to verify the effectiveness of the analysis. [ABSTRACT FROM AUTHOR]

Distributed phased Multiple-Input Multiple-Output (phased-MIMO) radar system consisting of separated subarrays possesses a high angle resolution but with sharing high sidelobes. This paper therefore focuses on the design of a virtual low-sidelobe beampattern in distributed phased-MIMO radar. An iterative framework with respect to subarray layout and receive weighting coefficients is proposed to minimize beampattern Peak Sidelobe Level (PSL) accounting for practical position constraints as well as the mainlobe level restriction. In each iteration, an efficient cyclic optimization algorithm based on the Coordinate Descent (CD) framework is developed to seek for the subarray layout through splitting high dimensional layout optimization problem into multiple one-dimensional optimization problems, and the SemiDefinite Relaxation (SDR) technique and rank one approximation are explored to design weighting coefficients. Numerical simulations are provided to assess the performance of the proposed algorithms in term of the achieved beampattern under different constraints and parameter settings. [ABSTRACT FROM AUTHOR]

This letter deals with a shaped beampattern synthesis problem through controlling the mainlobe ripple and the output filter energy based on the minimization of the peak sidelobe level in the spatial region of interest. To tackle the challenging optimization problem with nonconvex constraints, a novel first-order iterative convex approximation (FOICA) procedure that handles the nonconvex constraints with their convex upper bound functions resorting to the first-order Taylor expansion is developed. Hence, in each iteration, the original problem is approximated through a convex problem globally solved via a polynomial time method. Additionally, a nonnegative slack variable to ensure a decreasing trend in the objective function during the iteration is introduced. Finally, the numerical simulations are provided to verify that the proposed algorithm outperforms the state-of-the-art algorithms in terms of achieved beampattern performance. [ABSTRACT FROM AUTHOR]

Background: Colorectal cancer (CRC) is one of the third normal malignancy worldwide. Taurine-upregulated gene 1 (TUG1), a member of long noncoding RNAs (lncRNAs), has been reported to be involved in various cancers. However, the mechanism underlying TUG1 in the progression of CRC remains unclear. Methods: The expression of TUG1, microRNA-542-3p (miR-542-3p), and tribbles homolog 2 (TRIB2) in CRC tissues and cells (LoVo and HCT116) were detected by quantitative real-time PCR (qRT-PCR). Methyl thiazolyl tetrazolium (MTT), transwell and flow cytometry assays were employed to evaluate the effects of TUG1 in CRC cells. The interaction between miR-542-3p and TUG1 or TRIB2 were verified by dual-luciferase reporter assay. A xenograft tumor model in nude mice was established to investigate the biological role of TUG1 in CRC in vivo. Results: TUG1 was increased in CRC tissues and cells (LoVo and HCT116) in contrast with adjacent normal tissues and normal intestinal mucous cells (CCC-HIE-2). Downregulation of TUG1 or TRIB2 suppressed the proliferation, migration, invasion, and induced apoptosis in CRC cells. And knockdown of TUG1 repressed tumor growth in vivo. Besides, overexpression of TRIB2 reversed the effects of TUG1 depletion on the progression of CRC. Meanwhile, TUG1 interacted with miR-542-3p and TRIB2 was a target of miR-542-3p. Furthermore, miR-542-3p knockdown or TRIB2 overexpression partly reversed the suppression effect of TUG1 depletion on the Wnt/β-catenin pathway. Conclusions: TUG1 served as a tumor promoter, impeded the progression of CRC by miR-542-3p/TRIB2 axis to inactivate of Wnt/β-catenin pathway, which providing a novel target for CRC treatment. [ABSTRACT FROM AUTHOR]

Background: Circular RNAs (circRNAs) have been reported to play vital roles in colorectal cancer (CRC). However, only a few circRNAs have been experimentally validated and functionally described. In this research, we aimed to reveal the functional mechanism of circCSPP1 in CRC. Methods: 36 DOX sensitive and 36 resistant CRC cases participated in this study. The expression of circCSPP1, miR-944 and FZD7 were detected by quantitative real time polymerase chain reaction (qRT-PCR) and the protein levels of FZD7, MRP1, P-gp and LRP were detected by western blot. Cell proliferation, migration, invasion, and apoptosis were assessed by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay, transwell assay, or flow cytometry analysis, respectively. The interaction between miR-944 and circCSPP1 or frizzled-7 (FZD7) was predicted by Starbase 3.0 and verified by the dual luciferase reporter assay, RNA immunoprecipitation (RIP) assay and RNA pull down assay. Xenograft tumor assay was performed to examine the effect of circCSPP1 on tumor growth in vivo. Results: The expression of circCSPP1 and FZD7 was upregulated while miR-944 expression was downregulated in doxorubicin (DOX)-resistant CRC tissues and cells. CircCSPP1 knockdown significantly downregulated enhanced doxorubicin sensitivity, suppressed proliferation, migration, invasion, and induced apoptosis in DOX-resistant CRC cells. Interestingly, we found that circCSPP1 directly downregulated miR-944 expression and miR-944 decreased FZD7 level through targeting to 3′ untranslated region (UTR) of FZD7. Furthermore, circCSPP1 mediated DOX-resistant CRC cell progression and doxorubicin sensitivity by regulating miR-944/FZD7 axis. Besides, circCSPP1 downregulation dramatically repressed CRC tumor growth in vivo. Conclusion: Our data indicated that circCSPP1 knockdown inhibited DOX-resistant CRC cell growth and enhanced doxorubicin sensitivity by miR-944/FZD7 axis, providing a potential target for CRC therapy. [ABSTRACT FROM AUTHOR]

Grape white rot caused by Coniella diplodiella (Speg.) affects the production and quality of grapevine in China and other grapevine-growing countries. Despite the importance of C. diplodiella as a serious disease-causing agent in grape, the genome information and molecular mechanisms underlying its pathogenicity are poorly understood. To bridge this gap, 40.93 Mbp of C. diplodiella strain WR01 was de novo assembled. A total of 9,403 putative protein-coding genes were predicted. Among these, 608 and 248 genes are potentially secreted proteins and candidate effector proteins (CEPs), respectively. Additionally, the transcriptome of C. diplodiella was analyzed after feeding with crude grapevine leaf homogenates, which reveals the transcriptional expression of 9,115 genes. Gene ontology enrichment analysis indicated that the highly enriched genes are related with carbohydrate metabolism and secondary metabolite synthesis. Forty-three putative effectors were cloned from C. diplodiella , and applied for further functional analysis. Among them, one protein exhibited strong effect in the suppression of BCL2-associated X (BAX)-induced hypersensitive response after transiently expressed in Nicotiana benthamiana leaves. This work facilitates valuable genetic basis for understanding the molecular mechanism underlying C. diplodiella -grapevine interaction. [ABSTRACT FROM AUTHOR]

Background. CD147/basigin (Bsg), a transmembrane glycoprotein, activates matrix metalloproteinases and promotes inflammation. Objective. The aim of this study is to explore the clinical significance of CD147 in the pathogenesis of inflammatory bowel disease (IBD). Results. In addition to monocytes, the clinical analysis showed that there is no significance obtained in leucocyte, neutrophil, eosinophil, basophil, and erythrocyte between IBD and controls. Immunohistochemistry analysis showed that CD147 was increased in intestinal tissue of patients with active IBD compared to that in the control group. What is more, CD147 is involved in intestinal barrier function and intestinal inflammation, which was attributed to the fact that it has an influence on MCT4 expression, a regulator of intestinal barrier function and intestinal inflammation, in HT-29 and CaCO2 cells. Most importantly, serum level of CD147 content is higher in active IBD than that in inactive IBD or healthy control, which could be a biomarker of IBD. Conclusion. The data suggested that increased CD147 level could be a biomarker of IBD in children. [ABSTRACT FROM AUTHOR]

Background. Pyroptosis, a novel form of inflammatory programmed cell death, was recently found to be a cause of mucosal barrier defect. In our pervious study, CD147 expression was documented to increase in intestinal tissue of inflammatory bowel disease (IBD). Objective. The aim of this study was to determine the function of serum CD147 in pyroptosis. Methods. The study group consisted of 96 cases. The centration of CD147, IL-1β, and IL-18 levels in serum was assessed by ELISA. Real-time PCR and WB were performed to analyze the effect of CD147 on pyroptosis. Results. In this study, our results showed that CD147 induced cell pyroptosis in intestinal epithelial cells (IECs) by enhancement of IL-1β and IL-18 expression and secretion in IECs, which is attributed to activation of inflammasomes, including caspase-1 and GSDMD as well as GSDME, leading to aggregate inflammatory reaction. Mechanically, CD147 promoted phosphorylation of NF-κB p65 in IECs, while inhibition of NF-κB activity by the NF-κB inhibitor BAY11-7082 reversed the effect of CD147 on IL-1β and IL-18 secretion. Most importantly, serum CD147 level is slightly clinically correlated with IL-1β, but not IL-18 level. Conclusion. These findings revealed a critical role of CD147 in the patients with IBD, suggesting that blockade of CD147 may be a novel therapeutic strategy for the patients with IBD. [ABSTRACT FROM AUTHOR]

To explore the influence of spatially varying ground motion on the dynamic behavior of a train passing through a three-tower cable-stayed bridge, a 3D train–track–bridge coupled model is established for accurately simulating the train–bridge interaction under earthquake excitation, which is made up of a vehicle model built by multi-body dynamics, a track–bridge finite element model, and a 3D rolling wheel–rail contact model. A conditional simulation method, which takes into consideration the wave passage effect, incoherence effect, and site-response effect, is adopted to simulate the spatially varying ground motion under different soil conditions. The multi-time-step method previously proposed by the authors is also adopted to improve computational efficiency. The dynamic responses of the train running on a three-tower cable-stayed bridge are calculated with differing earthquake excitations and train speeds. The results indicate that (1) the earthquake excitation significantly increases the responses of the train–bridge system, but at a design speed, all the running safety indices meet the code requirements; (2) the incoherence and site-response effects should also be considered in the seismic analysis for long-span bridges though there is no fixed pattern for determining their influences; (3) different train speeds that vary the vibration characteristics of the train–bridge system affect the vibration frequencies of the car body and bridge. [ABSTRACT FROM AUTHOR]

Background: Mast cells (MCs) have been found to play a critical role during development of inflammatory bowel disease (IBD) that characterized by dysregulation of inflammation and impaired intestinal barrier function. However, the function of MCs in IBD remains to be fully elucidated. Results: In our study, we used exosomes isolated from human mast cells-1 (HMCs-1) to culture with NCM460, HT-29 or CaCO2 of intestinal epithelial cells (IECs) to investigate the communication between MCs and IECs. We found that MCs-derived exosomes significantly increased intestinal epithelial permeability and destroyed intestinal barrier function, which is attributed to exosome-mediated functional miRNAs were transferred from HMCs-1 into IECs, leading to inhibit tight junction-related proteins expression, including tight junction proteins 1 (TJP1, ZO-1), Occludin (OCLN), Claudin 8 (CLDN8). Microarray and bioinformatic analysis have further revealed that a panel of miRNAs target different tight junction-related proteins. Interestingly, miR-223 is enriched in mast cell-derived exosome, which inhibit CLDN8 expression in IECs, while treatment with miR-223 inhibitor in HT-29 cells significantly reversed the inhibitory effect of HMCs-1-derived exosomes on CLDN 8 expression. Most importantly, enrichment of MCs accumulation in intestinal mucosa of patients with IBD compared with those healthy control. Conclusions: These results indicated that enrichment of exosomal miR-223 from HMCs-1 inhibited CLDN8 expression, leading to destroy intestinal barrier function. These finding provided a novel insight of MCs as a new target for therapeutic treatment of IBD. [ABSTRACT FROM AUTHOR]

Background: The aim of the study is to investigate the effects of miR-34a targeted at PAI-1 on urinary microalbumin and renal function in hypertensive mice. Methods: Twenty specific-pathogen-free (SPF) BPN/3J mice were selected in normal group, and 120 SPF BPH/2J mice were evenly divided into model group, negative control group, miR-34a mimic group, miR-34a inhibitor group, Si-PAI-1 group, and miR-34a inhibitor + Si-PAI-1 group. qRT-PCR was used to detect the expression of miR-34a and PAI-1 mRNA. The protein expressions of PAI-1, angiotensin-converting enzyme (ACE) and ACE2 were detected by Western blot. Serum levels of AngII and Ang1-7 were detected by ELISA. Results: miR-34a negatively regulated the expression of PAI-1. Compared with the normal group, mice in the other groups had significantly lower body weight, increased systolic blood pressure and 24-h urinary microalbumin content, decreased miR-34a expression, superoxide dismutase (SOD) and nitric oxide (NO) content, and ACE2 protein expression, and increased PAI-1 expression, serum creatinine (Scr), blood urea nitrogen (BUN) malondialdehyde (MDA), AngII and Ang1-7 levels, and ACE protein expression (all P < 0.05). Compared with the model group, mice in the miR-34a mimic group and Si-PAI-1 group had no significant changes in body weight (all P > 0.05), while they had significantly lower systolic blood pressure and 24-h urinary microalbumin content, increased SOD and NO levels and ACE2 protein expression, and decreased PAI-1 expression, Scr, BUN, MDA, AngII and Ang1-7 levels, and ACE protein expression (all P < 0.05). Compared with the miR-34a inhibitor group, symptoms in miR-34a inhibitor + Si-PAI-1 group were significantly improved (all P < 0.05). Conclusions: miR-34a can inhibit the expression of PAI-1, thereby reducing urinary microalbumin content in hypertensive mice and protecting their renal function. [ABSTRACT FROM AUTHOR]

Cancer is always the greatest threat to human health. In this work, a split-ring resonator (SRR)-based sensor was simulated for label-free detection on biomarkers in microwave. The model of biosensor was simulated by Computer Simulation Technology 2017 (CST2017) and some influences of feature parameters on resonance frequency and Q-factor were also discussed. From the simulation results, the model of sensor in our work showed ultrahigh Q-factor and sensitivity under different permittivities and also exhibited a trend of better performance when it works in higher frequency. Therefore, the SRR-based biosensor will become an important application in cancer precaution and treatment in the future. [ABSTRACT FROM AUTHOR]

There are various proposals for the selection of the so-called 'objective' or 'default' priors in Bayesian analysis. The paper introduces a new criterion, the moment matching criterion, which requires the matching of the posterior mean with the maximum likelihood estimator up to a high order of approximation. A complete characterization of such priors in the one or multi-parameter case is provided. In the process, many new priors are derived. One interesting finding is that even in the absence of nuisance parameters, it is possible to find priors difierent from Jefireys' prior for a real valued parameter based on our criterion. [ABSTRACT FROM AUTHOR]

The paper revisits the problem of selection of priors for regular one-parameter family of distributions. The goal is to find some 'objective' or 'default' prior by approximate maximization of the distance between the prior and the posterior under a general divergence criterion as introduced by Amari (Ann Stat 10:357-387, 1982) and Cressie and Read (J R Stat Soc Ser B 46:440-464, 1984). The maximization is based on an asymptotic expansion of this distance. The Kullback-Leibler, Bhattacharyya-Hellinger and Chi-square divergence are special cases of this general divergence criterion. It is shown that with the exception of one particular case, namely the Chi-square divergence, the general divergence criterion yields Jeffreys' prior. For the Chi-square divergence, we obtain a prior different from that of Jeffreys and also from that of Clarke and Sun (Sankhya Ser A 59:215-231, 1997). [ABSTRACT FROM AUTHOR]

As a common depressant, acidified water glass (AWG) has strong inhibitory effects on fluorite and calcite. The inhibited fluorite is difficult to be recovered, thus resulting in the waste of resources and low flotation efficiency. In this study, the interaction of fluoride ions with fluorite and calcite surfaces was investigated, and its effects of AWG adsorption on mineral surfaces were evaluated. Micro-flotation experiments indicated that the flotation recovery of fluorite is 88.72% after fluoride ion treatment, that is, approximately fourfold with respect to that without fluoride ion modification. The results of solution chemical calculations showed that SiO(OH)3− is the main component to inhibit fluorite, and Si(OH)4 is the main component to inhibit calcite in AWG solution. XPS and ICP-MS results showed that fluoride ions can improve the floatability of fluorite by converting CaSiO3 on the surface of fluorite into CaF2, but the conversion ability of Si(OH)4 on the surface of calcite is weak, which increases the difference in floatability between fluorite and calcite. The above results were further verified by the analysis of flotation foam image and contact angle measurement. After fluorine ion treatment, the contact angle of fluorite increased, and it was more easily adsorbed on the foam. Therefore, the interaction of the fluoride ion with the fluorite surfaces eliminated the adsorption of AWG on fluorite, thereby resulting in the enhanced hydrophobicity of fluorite. Different from the traditional metal ions modification, the idea of anion modification in this paper is expected to be further studied. [ABSTRACT FROM AUTHOR]

Hub vane diffusers can obtain higher pressure recovery and efficiency compared with the vaneless diffuser and a wider operating range compared with the vane diffuser. To improve the performance of high pressure ratio centrifugal compressors it is necessary to understand the flow phenomena in the hub vane diffusers. Although some research work on hub vane diffuser flow has been published, many unknown flow physics and disputed questions still remain. A computational analysis of the flowfield in a high-speed centrifugal compressor with a backswept unshrouded impeller and wedge vane channel diffuser is presented. In order to obtain the optimum hub vane height, the pressure and velocity fields were numerically simulated for eight kinds of hub vane, which are varied as follows: h/b = 0 (vaneless), 0.2, 0.3, 0.4, 0.5, 0.6, 0.8 and 1 (vane). The advanced Navier-Stokes solver EURANUS/TURBO is applied with a Baldwin-Lomax model for closure. The calculations are performed with a central space discretization scheme, with second- and fourth-order artificial dissipation terms. The numerical procedure applied a four-stage explicit Runge-Kutta scheme, coupled to local time stepping. The good agreement of the computed stage performance and the experimental data of the stage with the vane diffuser shows that the solver is effective and reasonable. Numerical results show that the maximum flowrate attained by the stage with the vane diffuser is about 12.5 per cent less than with the hub vane diffusers. The stage efficiency reaches its maximum value for the hub vane diffuser with an h/b ratio of 0.5 at lower flowrate and for the hub vane diffuser with an h/b ratio of 0.4 at higher flowrate. The hub vane diffusers have a wider operating range than the vane diffuser. The hub vane diffuser with an h/b ratio of 0.3 shows a higher mass-averaged static pressure coefficient up to a flow coefficient φ = 0.16. The hub vane diffuser with an h/b ratio of 0.4 shows a slightly higher mass-averaged static pressure coefficient over an operating range of φ = 0.18-0.28, but it falls beyond φ = 0.3 below that of the vaneless diffuser. The performance of the centrifugal compressor with the shroud vane diffuser is better than that with the hub vane diffuser for the same height of the vane. [ABSTRACT FROM AUTHOR]

Objective: Inflammatory bowel disease (IBD) is a disorder intestinal inflammation and impaired barrier function, associated with increased epithelial expression of monocarboxylate transporter 4 (MCT4). However, the specific non‐metabolic function and clinical relevance of MCT4 in IBD remain to be fully elucidated. Methods: Lentivirus‐mediated overexpression of MCT4 was used to assess the role of MCT4 in transcriptionally regulating ZO‐1 and IL‐6 expression by luciferase assays, WB and ChIP. IP was used to analyse the effect of MCT4 on the interaction NF‐κB‐CBP or CREB‐CBP, and these MCT4‐mediated effects were confirmed in vivo assay. Results: We showed that ectopic expression of MCT4 inhibited ZO‐1 expression, while increased pro‐inflammatory factors expression, leading to destroy intestinal epithelial barrier function in vitro and in vivo. Mechanistically, MCT4 contributed NF‐κB p65 nuclear translocation and increased the binding of NF‐κB p65 to the promoter of IL‐6, which is attributed to MCT4 enhanced NF‐κB‐CBP interaction and dissolved CREB‐CBP complex, resulting in reduction of CREB activity and CREB‐mediated ZO‐1 expression. In addition, treatment of experimental colitis with MCT4 inhibitor α‐cyano‐4‐hydroxycinnamate (CHC) ameliorated mucosal intestinal barrier function, which was due to attenuation of pro‐inflammation factors expression and enhancement of ZO‐1 expression. Conclusion: These findings suggested a novel role of MCT4 in controlling development of IBD and provided evidence for potential targets of IBD. [ABSTRACT FROM AUTHOR]

Background: Although the genetic spectrum of human colorectal cancer (CRC) is mainly characterized by APC, KRAS and TP53 mutations, driver genes in tumor initiation have not been conclusively demonstrated. In this study, we aimed to identify novel markers for CRC. Methods: We performed exome analysis of sporadic colorectal cancer (sCRC) coding regions to screen loss of function (LoF) mutation genes, and carried out systems-level approaches to confirm top rank gene in this study. Results: We identified loss of BMP5 is an early event in CRC. Deep sequencing identified BMP5 was mutated in 7.7% (8/104) of sCRC samples, with 37.5% truncating mutation frequency. Notably, BMP5 negative expression and its prognostic value is uniquely significant in sCRC but not in other tumor types. Furthermore, BMP5 expression was positively correlated with E-cadherin in CRC patients and its dysregulation play a vital role in epithelial-mesenchymal transition (EMT), thus triggering tumor initiation and development. RNA sequencing identified, independent of BMP/Smads pathway, BMP5 signaled though Jak-Stat pathways to inhibit the activation of oncogene EPSTI1. Conclusions: Our result support a novel concept that the importance of BMP5 in sCRC. The tumor suppressor role of BMP5 highlights its crucial role in CRC initiation and development. [ABSTRACT FROM AUTHOR]