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Abstract—This study is devoted to the mycorrhizal colonization of three linden species (Tilia cordata, T. platyphyllos, and T. × europaea) in the urban environment of St. Petersburg. The study was conducted on three model territories: the Summer Garden, Botanical Garden, and Duderhof Heights. During the study, data was obtained on the morphology and anatomy of the mycorrhizal structures in linden trees, molecular identification of the mycorrhizal symbionts, and the influence of certain soil characteristics on the diversity of ectomycorrhizal fungi (EMF). Light microscopy confirmed the double mycorrhizal colonization characteristic of the genus Tilia: arbuscular mycorrhiza (AM) and ectomycorrhiza (EM). In all the studied trees, the root tips had intensive mycorrhizal colonization. EM colonization rates varied depending on the linden species, but only slightly on the season and location, while AM colonization rates varied with the season, place and linden species. In some of the studied trees, the presence of fine root endophytes was found. nrITS was used for molecular identification of EM symbionts of linden trees. As a result, 58 EMF taxa were identified. The main ectomycorrhizal symbionts identified for Tilia were basidiomycetes Inocybe, Tomentella, Sebacina and Entoloma, and ascomycetes Tuber and Peziza. Among the identified EMF taxa, 13 taxa were observed for the first time for T. cordata; 12, for T. platyphyllos; and 8, for T. × europaea. The taxonomic diversity of EMF varied depending on the species of linden, season, and location of trees. To study the influence of soil characteristics on the composition of EMF communities, soil was analyzed for the concentration of nitrate nitrogen (N ) and mobile phosphorus (P2O5) and pH. Among the studied soil parameters, EMF biodiversity was mainly affected by changes in the mobile phosphorus and nitrate nitrogen values, and an increase in soil pH led to a depletion in the diversity of EMF taxa. [ABSTRACT FROM AUTHOR]