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The effects of light‐driven enhancement of Evans Blue dye complexes with blood plasma proteins were observed for the first time, both in vitro and in vivo. The possible background of the effect concerns the photochemical cis‐trans isomerization of the azo dye molecules. The effect was induced in the solution with a red laser with a wavelength of 638 nm, which corresponds to the peak of the dye absorption. The lifetime of the enhanced fluorescence is approximately 1 second and enables its use as an optically tagged molecular flow tracer for blood flow velocity measurements. Utilizing the effect, we performed for the first time the intravital molecular tagging velocimetry of the blood velocity in blood vessels in a living animal. The results of the measurements of the blood flow velocities in the cerebral veins of a group of healthy mice are presented. [ABSTRACT FROM AUTHOR]

Background: The molecular rotor 9-(2-Carboxy-2-cyanovinyl)julolidine (CCVJ) is presumed to have a sensitivity towards velocity or shear which is supposed to result in a change in fluorescence quantum yield. Furthermore, a previously reported photoisomeric behavior may contribute to the measured fluorescence intensity changes. The goal of this research was to examine the hypothesized behavior theoretically and experimentally from the perspective of fluid dynamics. Results: A correlation between stirring rate and intensity could not be established in the present experiments with a completely illuminated sample in contrast to previously reported experiments in spectrofluorometers. Experiments and theoretical models of a Poiseuille flow were in good agreement with the photoisomeric behavior but excluded the influence of shear. Further experiments in a flow chamber supported the photoisomery hypothesis as well. Conclusion: No experimental evidence for the influence of velocity on the fluorescence intensity of CCVJ was found. The hypothesis of shear sensitivity was excluded as well. The results are consistent with the photoisomeric behavior of CCVJ. [ABSTRACT FROM AUTHOR]

In this paper, a new type of molecular tagging velocimetry based on NH fluorescence was developed and validated for the velocity measurements of a high-enthalpy rarefied gas wind tunnel where the maximum flow velocity exceeds 6 km/s near the nozzle exit at 0.2 Pa. The feasibility of this technique using the short-lived NH fluorescence was demonstrated in the hypersonic rarefied gas flow with yielding velocity profiles at multiple downstream locations from the nozzle exit. The total uncertainty of the measured flow velocities was estimated to be less than 6% when the flow velocity is above 2000 m/s. For the new tagging technique, only a single laser and a single time-gated camera are required for velocity measurement, due to the existence of NH radicals in the arc-discharged N mixed with a small amount of H. Therefore, the NH-MTV not only shows great promise for tagging in high-enthalpy rarefied gas of nitrogen or air flows without seeding, but also possesses high practicability and facility for velocity measurement. [ABSTRACT FROM AUTHOR]

The molecular tagging velocimetry (MTV) is a well-suited technique for velocity field measurement in gas flows. Typically, a line is tagged by a laser beam within the gas flow seeded with light emitting acetone molecules. Positions of the luminescent molecules are then observed at successive times and the velocity field is deduced from the analysis of the tagged line displacement and deformation. However, the displacement evolution is expected to be affected by molecular diffusion, when the gas is rarefied. Therefore, there is no direct and simple relationship between the velocity field and the measured displacement of the initial tagged line. This paper addresses the study of tracer molecules diffusion through a background gas flowing in a channel delimited by planar walls. Tracer and background species are supposed to be governed by a system of coupled Boltzmann equations, numerically solved by the direct simulation Monte Carlo (DSMC) method. Simulations confirm that the diffusion of tracer species becomes significant as the degree of rarefaction of the gas flow increases. It is shown that a simple advection-diffusion equation provides an accurate description of tracer molecules behavior, in spite of the non-equilibrium state of the background gas. A simple reconstruction algorithm based on the advection-diffusion equation has been developed to obtain the velocity profile from the displacement field. This reconstruction algorithm has been numerically tested on DSMC generated data. Results help estimating an upper bound on the flow rarefaction degree, above which MTV measurements might become problematic. [ABSTRACT FROM AUTHOR]

Particle image velocimetry (PIV) has become a powerful tool for flow velocity measurements in wind tunnel testing. However, it is generally difficult to apply the PIV technique to supersonic flows because of unreliable particle traceability. In the present study, the PIV and MTV (molecular tagging velocimetry) techniques are applied to transonic and supersonic flows, in which a normal shock wave appears, to evaluate particle traceability. Based on this work, it is found that the PIV data largely deviate from the MTV data behind a normal shock wave for both flows. The drag coefficient is also estimated for a particle from the velocity data measured by the two techniques. Its value is then compared to the drag coefficient value calculated from an empirical formula for particle Mach numbers ranging between 0.1 and 0.9. Based on the results, it is found that the experimental data can be reproduced reasonably well by the formula for particle Reynolds numbers higher than ~1. However, the data associated with particle Reynolds numbers lower than ~1 deviate largely from the formula. Graphical abstract: [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]

This review article reports the recent progress in the development of a new group of molecule-based flow diagnostic techniques, which include molecular tagging velocimetry (MTV) and molecular tagging thermometry (MTT), for both qualitative flow visualization of thermally induced flow structures and quantitative whole-field measurements of flow velocity and temperature distributions. The MTV and MTT techniques can also be easily combined to result in a so-called molecular tagging velocimetry and thermometry (MTV&T) technique, which is capble of achieving simultaneous measurements of flow velocity and temperature distribution in fluid flows. Instead of using tiny particles, the molecular tagging techniques (MTV, MTT, and MTV&T) use phosphorescent molecules, which can be turned into long-lasting glowing marks upon excitation by photons of appropriate wavelength, as the tracers for the flow velocity and temperature measurements. The unique attraction and implementation of the molecular tagging techniques are demonstrated by three application examples, which include: (1) to quantify the unsteady heat transfer process from a heated cylinder to the surrounding fluid flow in order to examine the thermal effects on the wake instabilities behind the heated cylinder operating in mixed and forced heat convection regimes, (2) to reveal the time evolution of unsteady heat transfer and phase changing process inside micro-sized, icing water droplets in order to elucidate the underlying physics pertinent to aircraft icing phenomena, and (3) to achieve simultaneous droplet size, velocity and temperature measurements of 'in-flight' droplets to characterize the dynamic and thermodynamic behaviors of flying droplets in spray flows. [ABSTRACT FROM AUTHOR]

Recent advances in ultra-high repetition rate (100 kHz and above) laser diagnostics for fluid dynamic measurements are reviewed. The development of the pulse burst laser system, which enabled several of these advances, is described. The pulse burst laser system produces high repetition rate output by slicing the output of a low power continuous wave laser and passing the resulting burst of pulses through a series of pulsed Nd:YAG amplifiers. Several systems have been built with output approaching 1.0 J/pulse over bursts of up to 100 pulses generated at between 50 and 1000 kHz. Combined with the capabilities of several types of commercially available high-speed cameras, these systems have been used to make a wide variety of high repetition rate and 3D flow measurements. Several examples of various high repetition rate laser diagnostics are described, including flow visualization, filtered Rayleigh scattering, planar Doppler velocimetry, particle image velocimetry, planar laser induced fluorescence, molecular tagging velocimetry and 3D flow visualization. [ABSTRACT FROM AUTHOR]

Direct measurements of the slip velocity in rarefied gas flows produced by local thermodynamic non-equilibrium at the wall represent crucial information for the validation of existing theoretical and numerical models. In this work, molecular tagging velocimetry (MTV) by direct phosphorescence is applied to argon and helium flows at low pressures in a 1-mm deep channel. MTV has provided accurate measurements of the molecular displacement of the gas at average pressures of the order of 1 kPa. To the best of our knowledge, this work reports the very first flow visualizations of a gas in a confined domain and in the slip flow regime, with Knudsen numbers up to 0.014. MTV is cross-validated with mass flowrate measurements by the constant volume technique. The two diagnostic methods are applied simultaneously, and the measurements in terms of average velocity at the test section are in good agreement. Moreover, preliminary results of the slip velocity at the wall are computed from the MTV data by means of a reconstruction method. [ABSTRACT FROM AUTHOR]