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Copyright of Veterinary Dermatology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: The aim of this study was to evaluate the histological tolerance of medical cyanoacrylate adhesives (CAs), butyl‐CA and octyl‐CA, applied by two different methods in surgical skin incisions of T. scripta. Methods: A two‐trial controlled blind study each one including 12 female adult T. scripta turtles. Two 1‐cm long full‐thickness surgical skin incisions were made on the dorsal aspect of each hind limb. In trial 1 CAs were applied superficially over the apposed wound edges; each animal's four wounds were closed using butyl‐CA, octyl‐CA and ethyl‐CA or absorbable suture as controls. In trial 2 adhesives were applied holding wound edges everted, and octyl‐CA was replaced by a non‐sutured control wound. At each time point (2, 7, 14 and 21 days) all wounds from three randomly chosen animals were biopsied. Results: Medical grade CAs did not induce any sign of tissue damage and/or inflammatory reaction attributable to histotoxicity independently of the method of application. Wound dehiscence rate was higher when CAs were applied superficially over the apposed wound edges. Conclusions: Octyl and butyl‐CA are well tolerated by turtles' soft skin but because of their high rate of dehiscence they are not recommended in high tension wounds; use in low tension wounds needs further investigation. [ABSTRACT FROM AUTHOR]

Background: The aim of this study was to evaluate the histological tolerance of medical cyanoacrylate adhesives (CAs), butyl‐CA and octyl‐CA, applied by two different methods in surgical skin incisions of T. scripta. Methods: A two‐trial controlled blind study each one including 12 female adult T. scripta turtles. Two 1‐cm long full‐thickness surgical skin incisions were made on the dorsal aspect of each hind limb. In trial 1 CAs were applied superficially over the apposed wound edges; each animal's four wounds were closed using butyl‐CA, octyl‐CA and ethyl‐CA or absorbable suture as controls. In trial 2 adhesives were applied holding wound edges everted, and octyl‐CA was replaced by a non‐sutured control wound. At each time point (2, 7, 14 and 21 days) all wounds from three randomly chosen animals were biopsied. Results: Medical grade CAs did not induce any sign of tissue damage and/or inflammatory reaction attributable to histotoxicity independently of the method of application. Wound dehiscence rate was higher when CAs were applied superficially over the apposed wound edges. Conclusions: Octyl and butyl‐CA are well tolerated by turtles' soft skin but because of their high rate of dehiscence they are not recommended in high tension wounds; use in low tension wounds needs further investigation. [ABSTRACT FROM AUTHOR]

Background: Compared with mammals, wound healing in reptiles is characterized by reduced wound contraction and longer healing times. The aim of this study is to describe the clinical and histopathological effects of topical insulin on second-intention healing of experimentally induced wounds in skin without dermal bony plates of Trachemys scripta elegans exposed to daily variations in ambient temperature and in an aquatic environment. Forty-four healthy adult females were assigned to two groups: Group 1 (n = 24) was used to assess clinical features such as wound contraction; Group 2 (n = 20) was used for histological evaluation and morphometric analysis. Topical porcine insulin (5 IU/ml diluted in glycerol) was applied daily 1 week. For each control time (2, 7, 14, 21 and 28 days post-wounding), re-epithelisation and wound remodelling were evaluated histologically and the number of main inflammatory cells (heterophils, macrophages, lymphocytes and fibroblasts) was scored. Results: Mean wound contraction was higher in the insulin-treated group at each time point and differences were significant at day 28 (P < 0.0001). Histologically, these clinical findings were associated with better re-epithelisation, inflammatory response, collagen synthesis and remodelling of the wounds. Morphometrically, insulin-treated wounds had significantly higher mean counts of heterophils (day 7), macrophages (days 2, 7 and 14) and fibroblasts (days 14 and 21), whereas lymphocyte counts were significantly lower at day 21. These results demonstrate that topical insulin modifies the inflammatory response of turtle skin up-regulating inflammatory cells at early stages and promoting wound healing. Conclusions: Topical insulin is a potentially useful therapy in skin wounds of Trachemys scripta and should be evaluated in non-experimental wounds of turtles and other reptiles. [ABSTRACT FROM AUTHOR]

Copyright of Veterinary Dermatology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Veterinary Dermatology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Veterinary Dermatology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract The aim of this study was to evaluate the utility of three monoclonal antibodies (mAbs), and two anticanine IgG and one anticanine IgM polyclonal antibodies (pAbs) for the immunohistochemical diagnosis of canine autoimmune skin diseases. Skin biopsies from 11 cases of pemphigus (7 foliaceus, 3 vulgaris and 1 erythematosus), 12 cases of discoid lupus erythematosus (DLE) and 12 cases of chronic hyperplasic dermatitis were used. The CA4E7 mAb (IgG1 + IgG2 ) showed similar sensitivity, but higher specificity and lower background than the two anti-IgG pAbs for the immunohistochemical diagnosis of pemphigus and DLE. The CA4F1 mAb (IgG2 ) and CA3H1 mAb (IgG2 ) showed moderate and low interepithelial reactivity, respectively, in autoimmune skin diseases, but strong staining of the cytoplasm of plasma cells of the inflammatory infiltrates. These results suggest that the CA4E7 mAb may be valuable in the immunohistochemical diagnosis of such disorders. [ABSTRACT FROM AUTHOR]