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Simple Summary: This study represents the first investigation of the seasonal dynamics and species diversity of Drosophilidae in Moscow, Russia, from 2021 to 2023. Traps were placed on the ground under trees to collect the specimens. Among the collected species, Drosophila obscura Fll., D. phalerata Mg., and D. testacea Roser were the most abundant. Peaks in the abundance of drosophilids varied between years, but the lowest abundance was always observed in May. In 2022, the highest number of flies was collected (9604 specimens), and the highest species diversity (33 species) was recorded. The effects of temperature and precipitation on the species abundance and community diversity indices are evident during the preimaginal developmental stages of drosophila. The insect fauna of natural parks in large cities has not been sufficiently studied in Russia. This study represents the first investigation of the seasonal dynamics and species diversity of Drosophilidae in Moscow city. Traps with fermenting liquid were placed on the ground under trees to collect flies from four natural park sites between early May and late September from 2021 to 2023. A total of 26,420 individuals belonging to 11 genera and 33 drosophilid species were identified, with 21 species from 6 genera being new to the fauna of Moscow. Drosophila obscura Fll., D. phalerata Mg., and D. testacea Roser were the most abundant species in the traps. Peaks in the abundance of drosophilids varied between years, but the lowest abundance was always observed in May. In 2022, the highest number of flies was collected (9604 specimens), with slightly fewer in 2023 (8496 specimens), and even fewer in 2021 (8320 specimens). In 2022, the highest species diversity of drosophilids was also recorded—33 species—while 28 species were found in both 2021 and 2023. The high variability in the abundance of individual drosophila species obscures the differences between the studied years due to the effects of the "Month" and "Site" factors. The diversity metrics exhibit similar patterns among drosophila communities inhabiting comparable biotopes. Specific climatic factors, such as the temperature and precipitation, impact the species abundance and community diversity indices primarily through their effects on the preimaginal stages of drosophila development. For several species, the population dynamics in the spring, post-hibernation, are influenced by the conditions preceding winter. [ABSTRACT FROM AUTHOR]

Microtubules are an indispensable component of all eukaryotic cells due to their role in mitotic spindle formation, yet their organization and number can vary greatly in the interphase. The last common ancestor of all eukaryotes already had microtubules and microtubule motor proteins moving along them. Sponges are traditionally regarded as the oldest animal phylum. Their body does not have a clear differentiation into tissues, but it contains several distinguishable cell types. The choanocytes stand out among them and are responsible for creating a flow of water with their flagella and increasing the filtering and feeding efficiency of the sponge. Choanocyte flagella contain microtubules, but thus far, observing a developed system of cytoplasmic microtubules in non-flagellated interphase sponge cells has been mostly unsuccessful. In this work, we combine transcriptomic analysis, immunofluorescence, and electron microscopy with time-lapse recording to demonstrate that microtubules appear in the cytoplasm of sponge cells only when transdifferentiation processes are activated. We conclude that dynamic cytoplasmic microtubules in the cells of sponges are not a persistent but rather a transient structure, associated with cellular plasticity. [ABSTRACT FROM AUTHOR]

The ability to regulate oxygen consumption evolved in ancestral animals and is intrinsically linked to iron metabolism. The iron pathways have been intensively studied in mammals, whereas data on distant invertebrates are limited. Sea sponges represent the oldest animal phylum and have unique structural plasticity and capacity to reaggregate after complete dissociation. We studied iron metabolic factors and their expression during reaggregation in the White Sea cold-water sponges Halichondria panicea and Halisarca dujardini. De novo transcriptomes were assembled using RNA-Seq data, and evolutionary trends were analyzed with bioinformatic tools. Differential expression during reaggregation was studied for H. dujardini. Enzymes of the heme biosynthesis pathway and transport globins, neuroglobin (NGB) and androglobin (ADGB), were identified in sponges. The globins mutate at higher evolutionary rates than the heme synthesis enzymes. Highly conserved iron-regulatory protein 1 (IRP1) presumably interacts with the iron-responsive elements (IREs) found in mRNAs of ferritin (FTH1) and a putative transferrin receptor NAALAD2. The reaggregation process is accompanied by increased expression of IRP1, the antiapoptotic factor BCL2, the inflammation factor NFκB (p65), FTH1 and NGB, as well as by an increase in mitochondrial density. Our data indicate a complex mechanism of iron regulation in sponge structural plasticity and help to better understand general mechanisms of morphogenetic processes in multicellular species. [ABSTRACT FROM AUTHOR]

Influence of the chitosan concentration in the low-concentrated acidic hydrogels formed by (bio)polyelectrolyte chitosan–gelatin complexes (at a constant gelatin concentration of 1%) was studied by shearing in steady flow and linear oscillations. These complexes, including native gelatin, demonstrate clearly expressed viscoelastic properties. Viscoelastic properties correlated well with the non-Newtonian behavior of hydrogels (according to the Cox–Merz rule). Increasing the chitosan concentration (from 0.1% to 0.6%) results in exponential growth of the apparent viscosity, yield stress, and storage modulus. However, a further increase in chitosan concentration to 0.8% leads to a reduction in these rheological parameters due to the electrostatic repulsion of similarly charged polyelectrolyte complexes under the high concentration of these complexes. The macro-rheological properties of chitosan–gelatin gels are mainly determined by the colloidal structure of sol-precursors in solutions. The yield stress dependence on the radius of the dispersed particles is of square type. Electron photomicrographs showed that the introduction of even small quantities of chitosan leads to radical changes in the supramolecular structure of the gelatin gel. [ABSTRACT FROM PUBLISHER]

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We present angular-resolved photoemission investigations of the Shockley-type surface state on the reconstructed (111) surface of gold. The so-called herringbone reconstruction, with three different 22×3[sup 1/2]domain orientations, forms a super-lattice that has a clearly observable influence on the surface electronic structure. In the L gap of the projected bulk states, there appears a non-uniform photoemission intensity distribution due to the back-folding of the Shockley state at the Bragg planes of the reduced surface Brillouin zone. Furthermore, there is a clear indication of the existence of surface-state band gaps in the electronic density of states of the Shockley state. [ABSTRACT FROM AUTHOR]

Resolving the 3D wave vector k in photoemission mapping of the band structure E(k) requires knowledge of the unoccupied final states. Both dispersions and lifetimes of these states can be achieved by very-low-energy electron diffraction (VLEED). By incorporating the non-free-electron and excitedstate effects in the final states, combining VLEED with photoemission provides accurate mapping of the valence E(k) resolved in the 3D wave vector and under control of the intrinsic accuracy. We here concentrate on the most accurate combined method which uses angle-dependent VLEED and photoemission measurements. It provides access to many Brillouin-zone lines using one crystal surface, and benefits from an intensity gain and a better intrinsic accuracy near the Fermi level. [ABSTRACT FROM AUTHOR]

We report on self-energy effects in the electronic structure of Cu as a prototype weakly correlated system containing electron states of different localization. The unoccupied and occupied excited states were mapped fully resolved in the three-dimensional k using very-low-energy electron diffraction and photoemission, respectively. The self-energy corrections to the density-functional theory show distinct band- and k-dependence. These results are well described by quasiparticle GW calculations, especially for less localized states. We find correlation of the self-energy effects with spatial overlap of the oneelectron wave function with the electron density, and elucidate the essential physics of this effect based on the electron-gas exchange correlation behavior. [ABSTRACT FROM AUTHOR]

Ferritins comprise a conservative family of proteins found in all species and play an essential role in resistance to redox stress, immune response, and cell differentiation. Sponges (Porifera) are the oldest Metazoa that show unique plasticity and regenerative potential. Here, we characterize the ferritins of two cold-water sponges using proteomics, spectral microscopy, and bioinformatic analysis. The recently duplicated conservative HdF1a/b and atypical HdF2 genes were found in the Halisarca dujardini genome. Multiple related transcripts of HpF1 were identified in the Halichondria panicea transcriptome. Expression of HdF1a/b was much higher than that of HdF2 in all annual seasons and regulated differently during the sponge dissociation/reaggregation. The presence of the MRE and HRE motifs in the HdF1 and HdF2 promotor regions and the IRE motif in mRNAs of HdF1 and HpF indicates that sponge ferritins expression depends on the cellular iron and oxygen levels. The gel electrophoresis combined with specific staining and mass spectrometry confirmed the presence of ferric ions and ferritins in multi-subunit complexes. The 3D modeling predicts the iron-binding capacity of HdF1 and HpF1 at the ferroxidase center and the absence of iron-binding in atypical HdF2. Interestingly, atypical ferritins lacking iron-binding capacity were found in genomes of many invertebrate species. Their function deserves further research. [ABSTRACT FROM AUTHOR]