1. Exploring the potential application of alternative nuclei in NMR based metabolomics.
- Author
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Sinclair, Georgia M., Oakes, Sophie M., Warden, Andrew C., Paten, Amy M., and Jones, Oliver A. H.
- Subjects
METABOLOMICS ,NUCLEAR magnetic resonance ,NUCLEAR magnetic resonance spectroscopy ,HONEYBEES ,CELL nuclei - Abstract
Introduction: Nuclear magnetic resonance (NMR) is widely used in metabolomics but it focusses on
1 H over other NMR-active nuclei. Objectives: To evaluate the potential of alternative NMR-sensitive nuclei to generate useful metabolomic data. Method: Proton, carbon, phosphorus, and nitrogen-based NMR metabolomics was undertaken on extracts from mint and European honey bee tissue. Results: Carbon NMR provided useful information but required larger sample sizes. Phosphorus produced overlapping peaks in one dimensional (1D) analysis but showed potential in 2D experiments.15 N NMR was found to not be sensitive enough for general metabolomic work. Conclusions: Alternative NMR active nuclei are useful for metabolomics. [ABSTRACT FROM AUTHOR]- Published
- 2023
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