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Hedgehog signaling is a highly conserved pathway that plays pivotal roles in morphogenesis, tumorigenesis, osteogenesis, and wound healing. Previous investigations in patients with Gorlin syndrome found low harm avoidance traits, and increased volumes in the cerebrum, cerebellum, and cerebral ventricles, suggesting the association between brain morphology and the constitutive hyperactivation of hedgehog signaling, while the changes of regional brain volumes in upregulated hedgehog signaling pathway remains unclear so far. Herein, we investigated comprehensive brain regional volumes using quantitative structural brain MRI, and identified increased volumes of amygdala, striatum, and pallidum on the global segmentation, and increased volumes of the lateral and medial parts of the central nucleus of the amygdala on the detail segmentation in Ptch heterozygous deletion mice. Our data may enhance comprehension of the association between brain morphogenic changes and hyperactivity in hedgehog signaling. [ABSTRACT FROM AUTHOR]

Lissencephaly (LIS) is a rare neurodevelopmental disorder with severe symptoms caused by abnormal neuronal migration during cortical development. It is caused by both genetic and non-genetic factors. Despite frequent studies about the cortex, comprehensive elucidation of structural abnormalities and their effects on the white matter is limited. The main objective of this study is to analyze abnormal neuronal migration pathways and white matter fiber organization in LIS1-associated LIS using diffusion MRI (dMRI) tractography. For this purpose, slabs of brain specimens with LIS (n = 3) and age and sex-matched controls (n = 4) were scanned with 3T dMRI. Our high-resolution ex vivo dMRI successfully identified common abnormalities across the samples. The results revealed an abnormal increase in radially oriented subcortical fibers likely associated with radial migration pathways and u-fibers and a decrease in association fibers in all LIS specimens. [ABSTRACT FROM AUTHOR]

Purpose: Galen's anastomosis, which is placed over the dorsal surface of the posterior cricoarytenoid muscle, is formed by the internal branch of the superior laryngeal nerve and the dorsal branch of the recurrent laryngeal nerve (RLN). This study aims to contribute literature about the prevalence of Galen's anastomosis which is still a controversial issue and indicates the prevalence of this type of anastomosis in both sides of the larynx and indicate the importance of this anastomosis in different neck surgeries and procedures. Subjects and Methods: Twenty-two adult formalin-fixed hemilaryngeal (11 larynx) specimens' anatomical micro-dissection were performed on both sides (right-left) posteriorly. Every specimen consisted of larynx, trachea, epiglottis, and hyoid bone. Internal laryngeal nerve, recurrent laryngeal nerve, and inferior thyroid artery were freed up connective tissue to better analyze their relationship and to find their exact course with termination point. Results: In our current study, total Galen's anastomosis prevalence was found %54.53 with %27.27 right single trunk, %18.18 left single trunk, 4.54 left plexus formation, and 4.54 left double trunk. There has not been any anastomotic structure in %22.72 of the left side and in %22.72 of the right side microdissection of laryngeal specimens. Conclusion: During laryngeal or thyroid surgery, observation of an unknown intralaryngeal branching pattern may lead to confusion and may increase the risk of iatrogenic nerve injury, thus it is crucial to contribute to the literature about microanatomy and prevalence of Galen's anastomosis. [ABSTRACT FROM AUTHOR]

Introduction: Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder characterized by deficits in social interaction, communication and repetitive, restrictive behaviors, features supported by cortical activity. Given the importance of the subventricular zone (SVZ) of the lateral ventrical to cortical development, we compared molecular, cellular, and structural differences in the SVZ and linked cortical regions in specimens of ASD cases and sex and age-matched unaffected brain. Methods: We used magnetic resonance imaging (MRI) and diffusion tractography on ex vivo postmortem brain samples, which we further analyzed by Whole Genome Bisulfite Sequencing (WGBS), Flow Cytometry, and RT qPCR. Results: Through MRI, we observed decreased tractography pathways from the dorsal SVZ, increased pathways from the posterior ventral SVZ to the insular cortex, and variable cortical thickness within the insular cortex in ASD diagnosed case relative to unaffected controls. Long-range tractography pathways from and to the insula were also reduced in the ASD case. FACSbased cell sorting revealed an increased population of proliferating cells in the SVZ of ASD case relative to the unaffected control. Targeted qPCR assays of SVZ tissue demonstrated significantly reduced expression levels of genes involved in differentiation and migration of neurons in ASD relative to the control counterpart. Finally, using genome-wide DNA methylation analyses, we identified 19 genes relevant to neurological development, function, and disease, 7 of which have not previously been described in ASD, that were significantly differentially methylated in autistic SVZ and insula specimens. Conclusion: These findings suggest a hypothesis that epigenetic changes during neurodevelopment alter the trajectory of proliferation, migration, and differentiation in the SVZ, impacting cortical structure and function and resulting in ASD phenotypes. [ABSTRACT FROM AUTHOR]

Rett syndrome (RTT) is a severe progressive neurodevelopmental disorder characterized by various neurological symptoms. Almost all RTT cases are caused by mutations in the X-linked methyl-CpG-binding protein 2 (MeCP2) gene, and several mouse models have been established to understand the disease. However, the neuroanatomical abnormalities in each brain region of RTT mouse models have not been fully understood. Here, we investigated the global and local neuroanatomy of the Mecp2 gene-deleted RTT model (Mecp2 -KO) mouse brain using T2-weighted 3D magnetic resonance imaging with different morphometry to clarify the brain structural abnormalities that are involved in the pathophysiology of RTT. We found a significant reduction in global and almost all local volumes in the brain of Mecp2 -KO mice. In addition, a detailed comparative analysis identified specific volume reductions in several brain regions in the Mecp2 -deficient brain. Our analysis also revealed that the Mecp2 -deficient brain shows changes in hemispheric asymmetry in several brain regions. These findings suggest that MeCP2 affects not only the whole-brain volume but also the region-specific brain structure. Our study provides a framework for neuroanatomical studies of a mouse model of RTT. [ABSTRACT FROM AUTHOR]

Background: Accurate knowledge of location and relation with different para- meters of the greater palatine foramen (GPF) is a crucial necessity in performing a variety of anaesthesiological, dental and surgical procedures. The main aim of this study was to identify the GPF's locations, numbers and shapes via associating with gender and palatal indices and compare with literature results.Materials and Methods: This study was held on the cranium collections of the many anatomy departments in Turkey. Various metric assessments were observed on sexed, dry, total of 97 craniums.Results: Thirty-eight male cranium observed and mean values of palatal indices was 86.28 ± 10.75 and for 48 female craniums mean value for palatal indices was 81.06 ± 10.56. Location of GPF observed bilaterally and mostly found near the third upper molar in either of both males and females. It was 62.7% (right), 60.9% (left) for male craniums and 49.0% (right) and 47.1% (left) for female craniums observed near the lateral border of upper third molar. GPF found oval shape for male craniums 62.8% (right) and 61.0% (left) and for female craniums 66.0% (right) and 66.0% (left).Conclusions: These results were compared with already existing anatomical data in other races and populations. These results would have great clinical influence in localising the palatine foramina toward better palatal area surgical approaches to and anaesthetise this area. [ABSTRACT FROM AUTHOR]

Museums are used in every discipline to collect, classify, and present information for scientific purposes. They also serve as an effective educational medium. Since the establishment of a boutique anatomy museum at Bahçeşehir University, lectures, conferences, and seminars have been organized there over the past four years on the history of human anatomy and the human body. In order to raise awareness about the need to make anatomy accessible to kindergarteners and school‐aged children, rather than exclusively to undergraduate students, activities that are suited to a wide range of ages have been developed at the museum and at the anatomy laboratory. Four different sessions were conducted, including activities such as lectures using plastic models as props, shaping organs out of playdough, anatomy puzzles, watching cartoons, and examining specimens through a microscope. Healthy and pathologic anatomies were chosen to match daily themes. Among the kindergarteners and elementary school children, no grading was done, nor was any questionnaire administered; however, a survey was administered in the 10–12 age group (N = 64). According to the students' written feedback, 93.75% said they "are happy with microscope activities" while 84.37% said they "had so much fun" participating in the playdough activities. However, 18.75% criticized the activities, saying they "could have been longer." In conclusion, it is believed that these "getting to know our bodies" activities that were hosted in the anatomy museum, including conferences, workshops, material preparation, and instructional movies, may play an important role in the development of a healthy society. [ABSTRACT FROM AUTHOR]

Background: Medical education in Ottoman-Turkish medicine was shaped by the influence of Claudius Galenus (c.130-c.210) and Ibn-i Sina (c.980-c.1037). These teachings were performed in madrasahs until the beginning of the 19th century. Within the scope of master-apprentice relationship, medical training was also given in private clinics. As a result of religious and social pressures on scientific studies, human cadavers were never used because they were considered sacred. For centuries, all books were handwritten in the Ottoman Empire until the first printing press was established in Istanbul during 1726. However, the first illustrated book on anatomy was not printed until another 100 years later.Materials and Methods: A comparative study was conducted on the anatomical drawings in "Miratü'l Ebdan Fi Teşrih-i Azau'l İnsan" (Ataullah ŞM, 17th), and significance of this book.Results: Forty-six out of 56 figures were received from the book of physician and anatomist Jean Palfin (1650-1730). Remaining 9 figures were cited from author himself as collected from Bernard Siegfried Albinus (1697-1170), Raymond Vieussens (1614-1715), R. Drake (1667-1707), Clopton Havers (1657-1702), Albrecht von Haller (1708-1777), Joseph Guichard Duverney (1648-1730). The figures were drawn exactly the same way with minimal changes. Main text of the book is mostly translation from Italian edition of Bertin and Palfin's Works. The book is not only the first printed anatomical book but also the first printed work in Ottoman-Turkish medicine. Another very significant aspect of the illustrations are perspective drawing figures which differ from miniature style drawings of the past.Conclusions: Şânîzade Ataullah's work has a significant value not only for being the first printed illustrated anatomy book which makes it more approachable but also for providing anatomical drawings as illustrations not as miniature style painting. [ABSTRACT FROM AUTHOR]

Objective: This study was conducted to examine the influence of smoke exposure of variable duration on the ultrastructure of and histopathologic and morphologic alterations in the olfactory epithelium. Methods: A total of 24 Wistar albino rats were randomly assigned to three groups and fed a standard rat chow and tap water. Experimental rats in groups I and II were exposed to cigarette smoke in a glass cabin over a period of 2 months for 5 or 15 min, respectively, four times daily; control rats (group III) were not exposed to cigarette smoke. After dissection, all tissue specimens were processed using routine procedures for TEM. Results: Groups I and II exhibited the presence of intraepithelial inflammatory cells and especially deep invaginations in the nuclear membrane of supporting cells. Extended intercellular spaces, cytoplasmic protrusions on the apical surface of supporting cells, atrophy of microvilli and olfactory neuron cilia as well as numerous electron-dense granular structures and lysosome-like structures were observed to an increasing degree from group I to group II. Particularly in group II, both supporting cells and olfactory neurons exhibited a cytoplasmic edema, mitochondrial degeneration, and numerous vacuolar structures, as well as apoptotic and minimal necrotic changes. In this group, hyperplasia of basal cells was also observed. Conclusion: Our electron microscopic findings show that cigarette smoke leads to toxic degenerative changes in the rat olfactory mucosa. [ABSTRACT FROM AUTHOR]

The study aimed to discover some variables influencing the risk of nicotine dependence in the Anatolian population. We examined 10 candidate genes and analysed 10 single nucleotide polymorphism (SNP) loci in 50 cases and 50 controls in a preliminary study. The main purpose of this work was to provide predicative definition in this regard. Buccal swab samples were collected from 50 cases and 50 controls, who had volunteered to participate in the study. The Fagerström Test for Nicotine Dependence (FTND) score of 4 or more was required for the case group. The control group was strictly chosen from people who had an FTND score of 0. SNP loci were genotyped and variations were observed in five SNP loci (rs16969968, rs806380, rs3733829, rs6474412, rs1329650). Based on these results, polymorphism was identified in the Turkish population in five SNP loci (rs16969968, rs806380, rs6474412, rs37333829, rs1329650). Although our findings indicated a non-significant association between the 10 selected loci and nicotine dependence in the Anatolian population, we believe that with a larger number of cases with five SNP loci, the variance we find will provide significant results for both forensic geneticists and psychiatrists. Since, to our knowledge, this is the first work to explore the potential association of these SNPs with nicotine addiction in the Anatolian population, the results should be expanded with larger sample groups. [ABSTRACT FROM AUTHOR]

Papier-mâché means chewed paper, and it defines a method. Various decorative products and functional tools have been produced with this method, which includes various techniques and materials. Maybe, the most interesting one among these is anatomic models developed and spread around the world by the French physician Louis Thomas Jerôme Auzoux (1797–1880) at the beginning of the nineteenth century. The purpose of this paper is to investigate the role of Dr Auzoux' human anatomical models in Ottoman–Turkish medicine. Primary and secondary sources were analysed such as Museum collections, archives, and scientific databases accessible on the Internet. This revealed that, at the beginning of the 1820s, Dr. Auzoux developed the method for papier-mâché anatomical models after a period of suffering difficulties in finding and preserving cadavers for dissection at the medical faculty which he worked. In 1825, he completed his invention, which had significant advantages over previously used methods for anatomical models, and then founded a production workshop in St. Aubin. Many medical schools in Europe, Africa, and South America utilised these models. Sources mentioned that the Ottoman Empire also purchased various anatomical models. Although it is not exactly known how many and from which models, it is known that whole male and female body models and pregnancy developmental models were purchased in 1837. In addition to human anatomic models, Dr. Auzoux's company also began to manufacture veterinary and botanical models soon. In that period of the Ottoman Empire during which cadaver dissection was forbidden and only artificial models and drawings were used for the education, Auzoux's models can be considered as very important tools for the Turkish Ottoman medical education and influential on the transition from traditional to modern medicine. Today, unfortunately, the fate of most of the human anatomical models purchased in the name of the Ottoman Empire is not known. [ABSTRACT FROM AUTHOR]

In this study, the normal incudomalleal (IM) joint surface of dogs was studied by SEM. The animals under the Ketamine + Xylazine (5/2 mg/kg. iv.) anesthesia, were perfused with 2.5% glutaraldehyde solution in 0.1 M sodium potassium phosphate buffer pH 7.2 via left ventricle. Middle ear ossicles were processed with routine histological procedures for SEM and examined under a Philips 505 type SEM. The articular surfaces of malleus and incus showed similar configuration and micromorphology. Characteristic appearance of the collagen fibers on the articular surfaces were not resolved at lower magnifications. On the other hand, at higher magnification, chondrocytes and their relationships with collagen fibers were observed clearly. In conclusion, this research has revealed that the incudomalleal joint in dog resembles the larger joints in human body. However, different configurations were observed in some regions of the joint surfaces. These differences can depend on the sound exposure of the animal over its lifetime. [ABSTRACT FROM AUTHOR]

Purpose: Precise knowledge of the level of the vocal fold as projected on the external thyroid cartilage is of critical importance for the performance of many surgical approaches. This study aims to identify the level of the anterior commissure, as well as the lengths of the vocal muscle and arytenoid cartilage in Turkish population. Materials and Method: Specimens were collected after autopsy from the Council of Forensic Medicine. One hundred human larynges (52 men, 48 women; age range: 25-80 years) were dissected under a stereomicroscope. Projection of the vocal fold was analyzed in relation to the superior thyroid (A) and the inferior border of the thyroid cartilage (B). Then, the larynx was dissected parallel to the level of the vocal fold to measure the length of the vocal muscle (C) and the length of the interarytenoid space (D). Results: The mean value of the "a" was 9.15 ± 1.99 mm in male and 9.38 ± 3.43 mm in female. Mean value of the "b" was 10.54 ± 1.73 mm and 8.88 ± 1.81 mm in male and female, respectively. The mean value of the parameter corresponding the length of vocal muscle which was "c" was found 15.00 ± 3.18 mm in male and 12.88 ± 4.12 mm in female. The mean value of the interarytenoid space "d" was 8.31 ± 1.76 mm in male and 8.13 ± 1.90 mm in female. Comparing between genders, no statistical differences were observed in parameters of a, c, d, a + b, a + b/2 (P >.05). However, the difference with female and male for the parameters of b and c + d was statistically significant (P <.05). Conclusion: Our results indicate that the anterior commissure projects slightly above the midline height for male and at the level to slightly below in female subjects in Turkish population. [ABSTRACT FROM AUTHOR]