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Purpose: The aim of the article is to analyze the recycling levels achieved in Krakow and the municipalities of the Krakow Metropolis and to identify the factors that influence these results. In order to increase recycling rates in municipalities, a calculation method has been proposed that can help estimate the amount of biodegradable waste composted in households. Design/methodology/approach: To achieve the goal of analyzing recycling levels in municipalities, data from the Local Data Bank of the Central Statistical Office and the Małopolska Marshal's Office were used. The proposed method for calculating the mass of composted biodegradable waste was based on exemplary declarations on composting of biodegradable waste posted on municipal websites and indicators such as the amount of kitchen waste generated/person/year and the amount of green waste generated in kg/m2 of green areas. Findings: The analysis of recycling levels shows the problems that municipalities may have in achieving the limits set by the European Commission. Actions must be taken to increase recycling rates in municipalities, and one of them may be a proposed method for calculating the amount of composted biodegradable waste in households. Failure to achieve recycling levels will result in administrative penalties imposed by the Provincial Inspectorates of Environmental Protection (WIOŚ) and failure to meet sustainable development goals. Research limitations/implications: Municipalities have household declarations regarding composting of biodegradable waste. The continuation of the research concerned checking the correctness of data in municipalities on the size of plots and the area of green areas using satellite maps, e.g. geoportal.gov.pl Practical implications: The work uses an original calculation method, helpful in estimating the amount of biodegradable waste subjected to composting. Municipalities could use this method, which would help estimate the neglected amounts of this type of waste in households. By using the proposed method, the management of the biodegradable waste fraction would be more transparent and reliable, which would help increase the achieved recycling levels. Social implications: The implementation of the proposed solution could change the approach of waste producers to the segregation of municipal waste and influence greater attention during segregating biodegradable waste. Consequently, this would also improve waste management and improve the results in achieving sustainable development goals in municipalities. Originality/value: The article is an attempt of response to the need to achieve higher and higher levels of recycling. The proposed solution has implementation value and is addressed to organizations managing waste management in local government units. [ABSTRACT FROM AUTHOR]

A 4‐month‐old intact female German shepherd dog was presented with a history of postprandial regurgitation, a palpably distended cervical oesophagus after eating, and poor weight gain despite a ravenous appetite. Computed tomography angiography, esophagoscopy and echocardiography identified a persistent right aortic arch with a concurrent patent ductus arteriosus (PDA) causing extraluminal oesophageal compression leading to marked segmental megaoesophagus. A heart murmur was not detectable. A left lateral thoracotomy was performed to ligate and transect the PDA without complication. The dog was discharged with mild aspiration pneumonia which resolved with antimicrobial therapy. Twelve months post‐surgery the owners reported no regurgitation. [ABSTRACT FROM AUTHOR]

Rational waste management is an essential element in the process of transitioning the linear economy into a circular model. Those actions that are taken to achieve better results in this area are important for regional and local development as well as for the implementation of sustainable development goals. This article presents the requirements and rules for the functioning of waste management in Poland. Taking the structure of the system into account, the functioning of waste management in the municipality of Krakow was analyzed. Particular attention was paid to the problem of municipal waste segregation by the inhabitants of Krakow. As part of our own research, surveys were conducted among the employees of the Municipal Cleaning Company LLC (Miejskie Przedsiębiorstwo Oczyszczania w Krakowie – MPO Sp. z o.o.), which showed the scale of the deficiencies. The results of the research were helpful in proposing actions to increase the effectiveness of the waste-segregation system in Krakow. [ABSTRACT FROM AUTHOR]

The paper presents the results of research on the influence of used plasticizing system on the structural and thermal properties of thermoplastic starch (TPS). The thermoplastic starch granulate was obtained by extrusion of native starch in the presence of a plasticizing system using a twin-screw extruder. Glycerol and urea were used as plasticizers in various proportions. In order to evaluate the effectiveness of the starch plasticization process, changes in its chemical structure were analyzed by infrared spectroscopy (FTIR), gel permeation chromatography (GPC) and X-ray diffraction (XRD). TPS thermal stability was determined by thermogravimetric analysis (TGA). An improvement in the efficiency of the plasticization process has been found for a urea-containing plasticizing system compared to the composition of starch plasticized only with glycerol. In addition, the XRD analysis confirms the beneficial effect of urea on the inhibition of starch retrogradation process. [ABSTRACT FROM AUTHOR]

This work investigates the potential application of various sterilization methods for microorganism inactivation on the thermoplastic starch blend surface. The influence of the e-beam and UV radiation, ethanol, isopropanol and microwave autoclave on structural and packaging properties were studied. All the applied methods were successful in the inactivation of yeast and molds, however only the e-beam radiation was able to remove the bacterial microflora. The FTIR analysis revealed no significant changes in the polymer structure, nevertheless, a deterioration of the mechanical properties of the blend was observed. The least invasive method was the UV radiation which did not affect the mechanical parameters and additionally improved the barrier properties of the tested material. Moreover, it was proved that during the e-beam radiation the chain scission and cross-linking occurred. The non-irradiated and irradiated samples were subjected to the enzymatic degradation studies performed in the presence of amylase. The results indicated that irradiation accelerated the decomposition of material, which was confirmed by the measurements of weight loss, and mass of glucose and starch released to the solution in the course of biodegradation, as well as the FTIR and thermal analysis. [ABSTRACT FROM AUTHOR]

Background: Computed tomography (CT) is commonly used to investigate equine paranasal sinus disease, however, only limited information is available in the literature about the detailed CT appearance of equine paranasal sinus cysts. Objectives: To investigate if paranasal sinus cysts have specific characteristics in CT images that allow differentiation from other sinus diseases. Study design: Retrospective observational study. Methods: Evaluation and comparison of CT studies of eight horses with surgically and/or histopathologically confirmed paranasal sinus cysts and 10 horses with other confirmed paranasal sinus diseases. Results: A discrete hyperattenuating wall‐like structure was detected in the periphery of the sinus lesion in precontrast acquisition in 7/8 horses with paranasal sinus cysts. A similar wall‐like structure was detected in 3/10 horses with other sinus diseases, however, in contrast to horses with paranasal sinus cysts, two of these also had hyperattenuating regions within the contents of the sinus lesion. Bone destruction and formation affecting cancellous and cortical bone and dental disease were frequent in horses with paranasal sinus cysts. No significant difference in attenuation values was found when the fluid/soft tissue attenuation contents of lesions in horses with paranasal sinus cysts (mean 28.9 ± SD 9.2 HU) were compared with other sinus diseases when ethmoid haematomas were excluded (30.4 ± 12.9 HU, P =.8). Main limitations: Low number of cases. Conclusions: Detection of a hyperattenuating cystic wall is a helpful feature for identifying paranasal sinus cysts in CT images of horses. In contrast, measurement of attenuation values of the soft tissue/fluid contents of the sinus lesions was not helpful in identifying paranasal sinus cysts. [ABSTRACT FROM AUTHOR]

Poly(butylene succinate) (PBS) and polylactide (PLA) were blended in a co‐rotating twin‐screw extruder with various contents of PBS from 0 to 100 wt%. The effect of PBS content on the thermal and mechanical properties of PBS/PLA blends was investigated by using DSC, softening point measurements, a Charpy impact test and tensile testing. The Fourier transform infrared spectra showed that the polymers are immiscible, but the addition of PBS could modify the PLA structure in PBS/PLA blends by changing the content of amorphous and crystalline phases. In addition, the cold crystallization temperature of PLA in blends decreases in comparison with pure PLA, which shows that PBS could have a plasticizing effect on PLA. This is confirmed by the results of DSC analysis. The mechanical properties of the blends depend on the percentage of PBS addition. Typically, the mechanical properties of PBS/PLA blends are intermediate between the properties of the polyesters from which they are obtained. However, in some cases unexpected changes in mechanical properties of the blends were observed. For example, the elongation at break for a PBS/PLA blend containing 10 wt% PLA is higher than for pure PBS. © 2019 Society of Chemical Industry [ABSTRACT FROM AUTHOR]

Four different compositions of polylactide/thermoplastic starch blends (PLA/TPS blends) for application as drug carriers were examined. Initially, using cyanocobalamin (1.355 kDa) as a model compound, the blend with the highest starch content (wt. 60%) was selected for further research of mass transfer phenomenon. In this case, different concentrations of acetaminophen (0.151 kDa), doxorubicin hydrochloride (0.580 kDa) and cyanocobalamin (1.355 kDa) were used for determination of particular releasing profiles. Besides from the comparative analysis of obtained results, the values of the overall mass transfer coefficient (K) were calculated for each of tested drug molecules. Depending on the size and properties of used compound, determined values of the coefficient range from 10-11 to 10-13 m/s. Based on these outcomes, it could be stated that PLA/TPS blend selected in preliminary research, seems to be preferred material for fabrication of long-term drug delivery systems, which could be successfully applied for example in anti-cancer therapy. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate the usefulness of new software Pixel Flux (PXFX) for clinical evaluation of tissue perfusion in the field of reproduction in dogs. The experiment was performed on six adult Beagle dogs. Different organs and tissues of the animals were examined with the MyLab25 Gold ultrasound system. Blood flow in the ovary, testicle, prostate, the ramification of the penile artery, and the network of blood vessels of the pampiniform plexus were examined with the use of colour coded Doppler technique, and obtained data was evaluated with the PXFX software. The more objective digital evaluation of data obtained with colour Doppler sonography through the application of dynamic tissue perfusion measurements provides new opportunities for diagnosis, as well as continuous monitoring of the function of the examined tissues and organs. The use of PXFX software is strongly indicated as a tool in small animal practice as an additional method for evaluation of tissue perfusion, especially in the cases when other methods like pulsed wave Doppler techniques are difficult to be performed [ABSTRACT FROM AUTHOR]

Methanol is oxidized into the formaldehyde and formate and these processes are accompanied by free radicals' generation. Formaldehyde and free radicals induce chemical modifications of proteins, leading to changes in their structure and function. The aim of this paper has been to evaluate the effect of N-acetylcysteine and vitamin E derivative U83836E on free radicals' generation and protein modifications induced during acute methanol intoxication. U83836E is an analog of α-tocopherol and similarly protects cells against oxidative damage. Moreover, this compound has hydrophilic properties and can be dissolved in an aqueous phase of blood and interstitial fluid, and next, membranes readily take it up. This compound belonging to the benzopyran family contains the reactive trolox ring and possesses antioxidant properties. The ESR determination indicates the increase in free radicals' signal 6 and 12 h after intoxication. Methanol ingestion causes a significant decrease in GSH level (by about 35%) and a significant increase in the lipid peroxidation product malondialdehyde (by about 25%). During methanol metabolism the aromatic amino acids of proteins are modified—the amount of carbonyl groups is increased (by about 42%) and fluorescence intensity of tryptophan is statistically decreased (by about 30%). The increase (by about 200%) in bityrosine fluorescence is also observed. Moreover, a significant decrease in free sulphydryl (by about 40%) and amino groups (by about 30%) in liver proteins is observed during intoxication. This is accompanied by the loss of lysosomal protease-cathepsin B activity (by about 25%). N-acetylcysteine (in dose 150 mg/kg body weight) and U83836E (in dose 10 mg/kg body weight) prevent free radicals' generation to a similar degree. U83836E protects membrane phospholipids against peroxidation a little stronger than N-acetylcysteine (concentration of MDA is decreased by 9 to 20% in the U83836 group and by 7 to 14% in the N-acetylcysteine group compared to methanol group). However after treating methanol-intoxicated rats with N-acetylcysteine, the changes in protein modification parameters are significantly smaller than in the group receiving methanol alone and they are a little smaller than after U83836E application. These findings suggest that N-acetylcysteine and to a smaller degree U83836E protect protein from modification in methanol intoxication, which can prevent liver pathologies. [ABSTRACT FROM AUTHOR]

Earlier studies have demonstrated that human platelets contain the 20S proteasome, and its protein activator. However, understanding the potential role of the proteasome in human platelets requires a detailed knowledge about its chymotryptic-like activity, a crucial one for protein degradation in all eukaryotic cells. In this communication we have shown that human platelet 20S proteasome exhibited chymotryptic-like activity towards succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin as substrate at a broad pH range, with optimum between pH 7.5-8.0 and 5.0-5.5. These two activities were markedly inhibited by a 10 kmol/l concentration of two structurally unrelated proteasome inhibitors: lactacystin/β-lactone or benzyloxycarbonyl-Ile-Glu(O-tert.-butyl)-Ala-leucinal, but not by ebelactone B, an inhibitor of lysosomal cathepsin A/deamidase. The chymotryptic-like activity of the 20S proteasome against succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin was also significantly inhibited in platelets, after exposure of platelet-rich plasma to 10 μmol/l lactacystin and benzyloxycarbonyl-Ile-Glu(O-tert.-butyl)-Ala-leucinal for up to 60 min. This indicates that these inhibitors can enter platelets and selectively inhibit 20S proteasome activity. We also demonstrated for the first time by Western blot analysis that human platelets contain a proteasome activator, PA28, which is known to play a key role in antigen processing by significant stimulation of the proteasomal chymotryptic-like activity. Since the platelet 20S proteasome was also present in a latent form, this suggests that its activity may be regulated in vivo in human platelets. All these results can therefore be beneficial in future studies on the role of the 20S proteasome in platelet biology. [ABSTRACT FROM AUTHOR]

Summary It was demonstrated recently that the SeqA protein, a main negative regulator of Escherichia coli chromosome replication initiation, is also a specific transcription factor. SeqA specifically activates the bacteriophage λp R promoter while revealing no significant effect on the activity of another λ promoter, p L . Here, we demonstrate that lysogenization by bacteriophage λ is impaired in E. coli seqA mutants. Genetic analysis demonstrated that CII-mediated activation of the phage p I and p aQ promoters, which are required for efficient lysogenization, is less efficient in the absence of seqA function. This was confirmed in in vitro transcription assays. Interestingly, SeqA stimulated CII-dependent transcription from p I and p aQ when it was added to the reaction mixture before CII, although having little effect if added after a preincubation of CII with the DNA template. This SeqA-mediated stimulation was absolutely dependent on DNA methylation, as no effects of this protein were observed when using unmethylated DNA templates. Also, no effects of SeqA on transcription from p I and p aQ were observed in the absence of CII. Binding of SeqA to templates containing the tested promoters occurs at GATC sequences located downstream of promoters, as revealed by electron microscopic studies. In contrast to p I and p aQ , the activity of the third CII-dependent promoter, p E , devoid of neighbouring downstream GATC sequences, was not affected by SeqA both in vivo and in vitro . We conclude that SeqA stimulates transcription from p I and p aQ promoters in co-operation with CII by facilitating functions of this transcription activator, most probably by allowing more efficient binding of CII to the promoter region. [ABSTRACT FROM AUTHOR]

The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms of its potential clinical importance for future therapies of cutaneous diseases. Mammalian skin is not only a prominent melatonin target, but also produces and rapidly metabolizes the multifunctional methoxyindole to biologically active metabolites. In our methodology, chitosan/collagen (CTS/Coll)-contained biomaterials are blended with melatonin at different doses to fabricate biomimetic hybrid scaffolds. We use rat tail tendon- and Salmo salar fish skin-derived collagens to assess biophysical and cellular properties by (i) Fourier transform infrared spectroscopy—attenuated total reflectance (FTIR–ATR), (ii) thermogravimetric analysis (TG), (iii) scanning electron microscope (SEM), and (iv) proliferation ratio of cutaneous cells in vitro. Our results indicate that melatonin itself does not negatively affect biophysical properties of melatonin-immobilized hybrid scaffolds, but it induces a pronounced elevation of cell viability within human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF), and reference melanoma cells. These results demonstrate that this indoleamine accelerates re-epithelialization. This delivery is a promising technique for additional explorations in future dermatotherapy and protective skin medicine. [ABSTRACT FROM AUTHOR]