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The use of material with proper physical characteristics is considered a main criterion for treatment of paper works. In this study, Bacterial Cellulose Nanofiber (BCN) has been used in the form of nanocomposite into Hydroxypropyl Cellulose (Klucel-G), in order to evaluation of its physical characteristics. Klucel-G polymer is often used a common material for treatment of paper works. After preparing this Bio-Nanocomposite by using hydrosoluble casting method, were coated on paper samples by coater machine and film samples were also prepared. Paper samples color change behavior was evaluated by Reflective Spectrophotometry test (UV-Vis) and also Thermal behavior (Tg temperature) from nanocomposite dried films was studied by Differential Scanning Calorimetry technique (DSC). The results of this study showed that by adding this Nano-fiber improved Tg thermal behavior in aging conditions. As a result, the existence of this Nano-additive, as compared with pure Klucel-G, resulted in resistance against decrease of glass temperature of Klucel-G, and hence its permanence and durability. The results of Calorimetry also showed that color change behavior of paper samples was improved, because of the presence of this Nanofiber. [ABSTRACT FROM AUTHOR]